##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765421_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7089925 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.130021544656678 33.0 33.0 33.0 27.0 33.0 2 31.025865153721654 33.0 33.0 33.0 27.0 33.0 3 31.074646769888258 33.0 33.0 33.0 27.0 33.0 4 34.64535689728735 37.0 37.0 37.0 27.0 37.0 5 34.784571204914016 37.0 37.0 37.0 27.0 37.0 6 34.70895249244526 37.0 37.0 37.0 27.0 37.0 7 34.56725818679323 37.0 37.0 37.0 27.0 37.0 8 34.49523034446768 37.0 37.0 37.0 27.0 37.0 9 34.4360132159367 37.0 37.0 37.0 27.0 37.0 10-11 34.40621551003713 37.0 37.0 37.0 27.0 37.0 12-13 34.346587023134944 37.0 37.0 37.0 27.0 37.0 14-15 35.68761150505823 37.0 37.0 40.0 27.0 40.0 16-17 35.75007415452209 37.0 37.0 40.0 27.0 40.0 18-19 35.77292488989658 37.0 37.0 40.0 27.0 40.0 20-21 35.78880326660719 37.0 37.0 40.0 27.0 40.0 22-23 35.83390303846656 37.0 37.0 40.0 27.0 40.0 24-25 35.798672623476264 37.0 37.0 40.0 27.0 40.0 26-27 35.814337951388765 37.0 37.0 40.0 27.0 40.0 28-29 35.73722119768545 37.0 37.0 40.0 27.0 40.0 30-31 35.70325927284139 37.0 37.0 40.0 27.0 40.0 32-33 35.57042345299844 37.0 33.0 40.0 27.0 40.0 34-35 35.51242241631611 37.0 33.0 40.0 27.0 40.0 36-37 35.39138947167989 37.0 33.0 40.0 27.0 40.0 38-39 35.17612139761704 37.0 33.0 40.0 27.0 40.0 40-41 35.097642852357396 37.0 33.0 40.0 27.0 40.0 42-43 34.964100254939225 37.0 33.0 40.0 27.0 40.0 44-45 34.83453816507227 37.0 33.0 40.0 27.0 40.0 46-47 34.70895926261561 37.0 33.0 40.0 27.0 40.0 48-49 34.53442321322158 37.0 33.0 40.0 27.0 40.0 50-51 34.367720744577696 37.0 33.0 40.0 24.5 40.0 52-53 34.17753093297884 37.0 33.0 40.0 22.0 40.0 54-55 34.04584089394457 37.0 33.0 40.0 22.0 40.0 56-57 33.85105470086073 37.0 33.0 37.0 22.0 40.0 58-59 33.610216398621986 37.0 33.0 37.0 22.0 40.0 60-61 33.37673219392307 37.0 33.0 37.0 22.0 40.0 62-63 33.19947933158672 37.0 33.0 37.0 22.0 40.0 64-65 32.88681897199194 37.0 33.0 37.0 22.0 40.0 66-67 32.66653420170171 37.0 33.0 37.0 22.0 40.0 68-69 32.36751566483426 35.0 33.0 37.0 22.0 38.5 70-71 32.23064327196691 33.0 33.0 37.0 22.0 37.0 72-73 31.9523090864854 33.0 33.0 37.0 22.0 37.0 74-75 31.670817110195102 33.0 27.0 37.0 22.0 37.0 76-77 31.481779708530063 33.0 27.0 37.0 22.0 37.0 78-79 31.239016632757046 33.0 27.0 37.0 22.0 37.0 80-81 30.95442441210591 33.0 27.0 37.0 22.0 37.0 82-83 30.762110459560574 33.0 27.0 37.0 15.0 37.0 84-85 30.571022330983755 33.0 27.0 37.0 15.0 37.0 86-87 30.352285390889183 33.0 27.0 37.0 15.0 37.0 88-89 30.1607482448686 33.0 27.0 37.0 15.0 37.0 90-91 29.941515389796084 33.0 27.0 37.0 15.0 37.0 92-93 29.781046484976923 33.0 27.0 37.0 15.0 37.0 94-95 29.52253973067416 33.0 27.0 37.0 15.0 37.0 96-97 29.424180298099063 33.0 27.0 37.0 15.0 37.0 98-99 28.28913980049154 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23688359126211722 1101 2 0.27001392966866433 1101 3 0.31191813936032986 1101 4 0.35050534104440345 1101 5 0.28178611487201266 1101 6 0.25746405999501576 1101 7 0.2377167480467932 1101 8 0.2888899271496115 1101 9 0.168792959507293 1101 10-11 0.25917702352102623 1101 12-13 0.20434564025131863 1101 14-15 0.32356493141464426 1101 16-17 0.3013096476827428 1101 18-19 0.23541356174700212 1101 20-21 0.31162210225777187 1101 22-23 0.2283884866106547 1101 24-25 0.2981594911211829 1101 26-27 0.35872734138781937 1101 28-29 0.2983119819858686 1101 30-31 0.29828877643406315 1101 32-33 0.4230033582730215 1101 34-35 0.3224116103561343 1101 36-37 0.35460432115429086 1101 38-39 0.3401150929868706 1101 40-41 0.2952070935066544 1101 42-43 0.2858957549080543 1101 44-45 0.2899157394293326 1101 46-47 0.3111345758095325 1101 48-49 0.3443860465387658 1101 50-51 0.37821218775916066 1101 52-53 0.22447627661444614 1101 54-55 0.37897958417480027 1101 56-57 0.409472454264062 1101 58-59 0.35893064678248976 1101 60-61 0.40439814841781185 1101 62-63 0.4162582468571614 1101 64-65 0.2675372877300859 1101 66-67 0.3706942676287639 1101 68-69 0.29245805018167204 1101 70-71 0.36095551999389386 1101 72-73 0.3486904652442604 1101 74-75 0.28280864541686057 1101 76-77 0.18791497644843247 1101 78-79 0.27940109148077497 1101 80-81 0.18491081039921298 1101 82-83 0.1976400978981978 1101 84-85 0.13800898570668707 1101 86-87 0.11987141370792997 1101 88-89 0.19718224117533723 1101 90-91 0.1280147006041652 1101 92-93 0.2086715637457459 1101 94-95 0.32820367747596535 1101 96-97 0.24913419912116197 1101 98-99 0.16342623164411307 1102 1 0.23357424013928352 1102 2 0.30387306165485484 1102 3 0.2728918429634781 1102 4 0.3304281584046791 1102 5 0.29284223712310364 1102 6 0.30493681335963174 1102 7 0.23222805394603085 1102 8 0.26052647060848244 1102 9 0.24116661656814387 1102 10-11 0.24910226495918408 1102 12-13 0.25124136611720616 1102 14-15 0.3959344190615468 1102 16-17 0.35236353978218915 1102 18-19 0.35321036584272747 1102 20-21 0.30813724789636865 1102 22-23 0.16562251678557516 1102 24-25 0.33013317533171005 1102 26-27 0.39879453682667076 1102 28-29 0.215034919580404 1102 30-31 0.3233127263235289 1102 32-33 0.4172063214658266 1102 34-35 0.3212811173956638 1102 36-37 0.436320665257 1102 38-39 0.39860605602452637 1102 40-41 0.3129888683195148 1102 42-43 0.2900827191868416 1102 44-45 0.2819670910951686 1102 46-47 0.3897257987083407 1102 48-49 0.3745928688635516 1102 50-51 0.35156429961580926 1102 52-53 0.25029726331110425 1102 54-55 0.26122011087844754 1102 56-57 0.30900935842571187 1102 58-59 0.4028767546830423 1102 60-61 0.42535265332414696 1102 62-63 0.34165773489191054 1102 64-65 0.26003428187350863 1102 66-67 0.2684197068716827 1102 68-69 0.19787217964682213 1102 70-71 0.2570129997286017 1102 72-73 0.24668439535324183 1102 74-75 0.24238317126722464 1102 76-77 0.24190646308691655 1102 78-79 0.3171495109819986 1102 80-81 0.3011333213560441 1102 82-83 0.256066289574882 1102 84-85 0.24255791329462895 1102 86-87 0.3378337338413502 1102 88-89 0.33039181725259326 1102 90-91 0.3828902969273251 1102 92-93 0.4354081731395354 1102 94-95 0.35021158966616284 1102 96-97 0.29539345909866555 1102 98-99 0.28017709461583706 1103 1 0.10214974773370145 1103 2 0.18179150960087043 1103 3 0.19994785982249041 1103 4 0.2799067013115817 1103 5 0.2431542294922906 1103 6 0.18288396422091324 1103 7 0.11061108145465681 1103 8 0.1500875863947968 1103 9 0.22355191202306202 1103 10-11 0.09783976515156922 1103 12-13 0.1335173159445091 1103 14-15 0.13856358945993463 1103 16-17 0.08212553437688541 1103 18-19 0.1019540226024489 1103 20-21 0.13941887050128798 1103 22-23 0.16126055971265885 1103 24-25 0.2509165985245758 1103 26-27 0.3142797970477176 1103 28-29 0.23627902040334448 1103 30-31 0.24163563994397208 1103 32-33 0.31422660943509584 1103 34-35 0.2350214483610742 1103 36-37 0.28502099725405117 1103 38-39 0.2425689427391191 1103 40-41 0.21265994123032783 1103 42-43 0.24293705355584194 1103 44-45 0.15154828644263318 1103 46-47 0.1307581884922655 1103 48-49 0.22526131816151462 1103 50-51 0.29542722114828024 1103 52-53 0.18566088972892913 1103 54-55 0.16842465492431558 1103 56-57 0.11872800329868483 1103 58-59 0.2325362881899551 1103 60-61 0.28419534346119946 1103 62-63 0.21906610707470975 1103 64-65 0.22166266481365682 1103 66-67 0.24157342512636149 1103 68-69 0.17874461099589922 1103 70-71 0.23130581605448697 1103 72-73 0.2178673714462036 1103 74-75 0.11015604135492296 1103 76-77 0.1064121676873313 1103 78-79 0.09903548802715179 1103 80-81 0.09732030129770308 1103 82-83 0.11678549311474384 1103 84-85 0.2027406510286056 1103 86-87 0.2921290252736988 1103 88-89 0.19588331307798512 1103 90-91 0.18346024530783822 1103 92-93 0.2527784702812639 1103 94-95 0.22123066379331746 1103 96-97 0.170999304842546 1103 98-99 0.1839547310514007 1104 1 0.11235427696022171 1104 2 0.020039211151065217 1104 3 0.030913895591062612 1104 4 0.14354711559734312 1104 5 0.10291759624706742 1104 6 0.02284816355392394 1104 7 -0.11344915980311754 1104 8 -0.1018193666685292 1104 9 -0.09280457364275918 1104 10-11 0.0012160344728826544 1104 12-13 -0.029616537804898257 1104 14-15 0.010336997326007236 1104 16-17 0.03246577579562171 1104 18-19 -0.03175878364150009 1104 20-21 0.0527696575113481 1104 22-23 0.07960145456937084 1104 24-25 0.10440053049401854 1104 26-27 0.11938359198220638 1104 28-29 0.008104995179145646 1104 30-31 0.1280604709411577 1104 32-33 0.1765742153640133 1104 34-35 0.0922549553595644 1104 36-37 0.1553323305458818 1104 38-39 0.07816116962231234 1104 40-41 0.0749021194646744 1104 42-43 -0.009612889057528662 1104 44-45 -0.002581772527591397 1104 46-47 -0.032518361658425476 1104 48-49 0.05407410096726295 1104 50-51 0.09407331515487982 1104 52-53 -0.0796054127964183 1104 54-55 0.08675139869279036 1104 56-57 -0.0025178319609011623 1104 58-59 0.05156625672177029 1104 60-61 0.038455476395938604 1104 62-63 0.01676126882348683 1104 64-65 0.03244693198274007 1104 66-67 0.025832975988016926 1104 68-69 -0.030755341808763603 1104 70-71 -0.02780654971409291 1104 72-73 -0.020889935420196792 1104 74-75 -0.08055531540015792 1104 76-77 -0.2289542762871264 1104 78-79 -0.21331666127769822 1104 80-81 -0.123244696303626 1104 82-83 -5.080851280929721E-4 1104 84-85 -0.11850342431983663 1104 86-87 -0.02229070655625165 1104 88-89 0.0927627343016475 1104 90-91 0.059077931960732855 1104 92-93 0.10683160994689445 1104 94-95 -0.07712480276038391 1104 96-97 -0.3370643984977484 1104 98-99 -0.18176994233689214 1105 1 -0.05094012904468315 1105 2 0.04856039804218781 1105 3 0.05220724570926549 1105 4 -0.062471757201414846 1105 5 0.008166760942422968 1105 6 -0.05270578764280032 1105 7 -0.13048671387151245 1105 8 -0.14458257901029725 1105 9 -0.1347803221322792 1105 10-11 -0.10493635863675621 1105 12-13 -0.08356127140424974 1105 14-15 -0.0784536858415521 1105 16-17 -0.04357336590271643 1105 18-19 -0.05940097758301022 1105 20-21 7.9634219245861E-4 1105 22-23 0.027750814126619616 1105 24-25 0.04493450653551889 1105 26-27 0.04531592664115891 1105 28-29 -0.04445638131559804 1105 30-31 0.07022345666876362 1105 32-33 0.08296231249273944 1105 34-35 0.011390275529748806 1105 36-37 -0.02402495083816092 1105 38-39 -0.059685193693589156 1105 40-41 -0.11500136819632445 1105 42-43 -0.043061796129002516 1105 44-45 -0.04859547914266216 1105 46-47 -0.08114181323346514 1105 48-49 -0.11723446901612533 1105 50-51 -0.03222303766031587 1105 52-53 -0.10033201593847707 1105 54-55 0.02304537973287779 1105 56-57 0.09018514764723307 1105 58-59 -0.055348054727907936 1105 60-61 -0.03422919274657232 1105 62-63 0.040951723949845587 1105 64-65 -0.1275203349155234 1105 66-67 -0.04303864588244721 1105 68-69 -0.09101461656163679 1105 70-71 -0.16230146461812467 1105 72-73 -0.12224549280157504 1105 74-75 -0.008327860316516222 1105 76-77 -0.1289341709153362 1105 78-79 -0.0631588295874117 1105 80-81 -0.10042383407115452 1105 82-83 -0.0011455779901403673 1105 84-85 -0.041886704314986645 1105 86-87 0.031028182735497012 1105 88-89 -0.0041524339473220095 1105 90-91 0.03615558874979641 1105 92-93 0.05925973494355219 1105 94-95 0.006328448873443193 1105 96-97 -0.07457257457580369 1105 98-99 0.07457768394698405 1106 1 -0.005082123246701542 1106 2 0.08671165651308144 1106 3 0.006035182714235532 1106 4 0.006500819299439797 1106 5 -0.021867248771570758 1106 6 0.03916801224298894 1106 7 -0.022757287118402303 1106 8 -0.043443852122756255 1106 9 0.03259869257929893 1106 10-11 -0.011637381929567425 1106 12-13 -0.005455584498974986 1106 14-15 0.01096647769975334 1106 16-17 0.011975834968254162 1106 18-19 0.056503930093491306 1106 20-21 -0.07495101509540802 1106 22-23 -0.04711196185836286 1106 24-25 -0.030353489390449795 1106 26-27 0.02344908593395445 1106 28-29 -0.12144962827741779 1106 30-31 -0.06172921883683813 1106 32-33 0.06331553758585073 1106 34-35 -0.17918213090425894 1106 36-37 -0.12256013844335456 1106 38-39 -0.09408596182843354 1106 40-41 -0.06769722297200786 1106 42-43 -0.08750180357902337 1106 44-45 -0.12033136166309077 1106 46-47 -0.16989920095716116 1106 48-49 -0.239547961896605 1106 50-51 -0.10858105675492169 1106 52-53 -0.14932568618241504 1106 54-55 -0.1400959225163092 1106 56-57 -0.11969032822320003 1106 58-59 -0.09293634538078521 1106 60-61 0.02629032706644807 1106 62-63 -0.05627861056124317 1106 64-65 -0.12303539371558969 1106 66-67 -0.06558981407764009 1106 68-69 -0.08037871488480164 1106 70-71 -0.10574947529006806 1106 72-73 0.01406695765243171 1106 74-75 -0.05681575490373447 1106 76-77 -0.06569951032383514 1106 78-79 -0.06246690924891851 1106 80-81 -0.1312223694870731 1106 82-83 -0.029254339004555874 1106 84-85 -0.23034523110520055 1106 86-87 -0.2222104889448282 1106 88-89 -0.05547283146298909 1106 90-91 -0.11719188697516003 1106 92-93 -0.06183667153214856 1106 94-95 -0.237058805994387 1106 96-97 -0.20524522711160387 1106 98-99 0.10292068582142022 1107 1 0.042107037343456 1107 2 0.05911145116919414 1107 3 0.05468988640895489 1107 4 0.023181563032480312 1107 5 0.039167320886456025 1107 6 0.0036509229765187 1107 7 -0.09690489701552707 1107 8 0.015240994944115016 1107 9 -0.08347692201544987 1107 10-11 -0.01554205224656613 1107 12-13 -0.04248177141860765 1107 14-15 -0.04952467295028384 1107 16-17 -0.04809535962310463 1107 18-19 -0.0912428447032525 1107 20-21 -0.039335959305638823 1107 22-23 -0.06146604697620006 1107 24-25 0.037346376009679716 1107 26-27 0.008823685981170115 1107 28-29 -0.004914232295163856 1107 30-31 -0.03174107646705693 1107 32-33 -0.13777430313666628 1107 34-35 -0.13298294124196985 1107 36-37 -0.09675277766262269 1107 38-39 -0.07871381179001702 1107 40-41 -0.22385687597769532 1107 42-43 -0.21907874983546805 1107 44-45 -0.1387634613823323 1107 46-47 -0.2978867449764522 1107 48-49 -0.15449861022347733 1107 50-51 -0.05460568671322363 1107 52-53 -0.1767757280076765 1107 54-55 -0.19643703146351754 1107 56-57 -0.15275179472187972 1107 58-59 -0.05517344021837545 1107 60-61 0.03926916371998601 1107 62-63 -0.22418611940248212 1107 64-65 -0.35469632715653887 1107 66-67 -0.09327832460760277 1107 68-69 -0.10748692498687973 1107 70-71 -0.10606785239548344 1107 72-73 -0.12958129183407863 1107 74-75 -0.16533482244478392 1107 76-77 -0.1424850518986922 1107 78-79 -0.02797069188595458 1107 80-81 -0.3117468880359411 1107 82-83 -0.15079394119232958 1107 84-85 -0.1687993193675048 1107 86-87 -0.22402540275684046 1107 88-89 -0.1512133287021733 1107 90-91 -0.09040184456225475 1107 92-93 -0.0569353893764486 1107 94-95 -0.04619721237038377 1107 96-97 -0.15235152411587904 1107 98-99 0.16061279927707872 1108 1 -0.04935267922249764 1108 2 0.005628399807822149 1108 3 -0.09414263506817022 1108 4 -0.13433614324439702 1108 5 -0.08726442846003835 1108 6 -0.25145793179359544 1108 7 -0.07088932354749033 1108 8 -0.2162300075023751 1108 9 -0.15054377379406958 1108 10-11 -0.1981442637099846 1108 12-13 -0.16940252232329556 1108 14-15 -0.21919614869905502 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2215 92-93 0.2028747352770779 2215 94-95 0.2319742391536046 2215 96-97 0.20730761753657845 2215 98-99 0.02892485108734988 2216 1 0.006083601978417619 2216 2 -0.0249788153194892 2216 3 -0.015961338372441958 2216 4 0.024473531722193798 2216 5 -0.08252830469572814 2216 6 0.078036312378714 2216 7 0.044294113573819516 2216 8 -0.031362125126449314 2216 9 0.02351456562654164 2216 10-11 -0.016527332105063408 2216 12-13 -0.018258161739261425 2216 14-15 -0.07558251612877598 2216 16-17 -0.051354609682327634 2216 18-19 -0.06461585272858628 2216 20-21 -0.07482908641346597 2216 22-23 -0.21014458238303035 2216 24-25 -0.18624694234763695 2216 26-27 -0.14129113458461262 2216 28-29 -0.050125480552154045 2216 30-31 -0.07672616486699013 2216 32-33 -0.10387183945507417 2216 34-35 -0.1563942509735341 2216 36-37 -0.19093471243523652 2216 38-39 -0.11450137524160908 2216 40-41 -0.10274477318888131 2216 42-43 -0.09171845878523754 2216 44-45 -0.17669744694769918 2216 46-47 -0.006423285891088426 2216 48-49 0.08035524757904255 2216 50-51 -0.019598466047192176 2216 52-53 0.1100704419047176 2216 54-55 -0.0280543478164077 2216 56-57 0.10397003212061406 2216 58-59 -0.0808051712461193 2216 60-61 -0.009215289672106053 2216 62-63 0.02920269264061659 2216 64-65 0.15696304074793233 2216 66-67 0.07182776794408596 2216 68-69 -0.026041085808635955 2216 70-71 -0.008950117090535059 2216 72-73 0.015131154122766333 2216 74-75 0.08506870638843722 2216 76-77 0.037596785647281195 2216 78-79 0.12472074454463566 2216 80-81 0.2578978917525774 2216 82-83 0.1688179090753259 2216 84-85 0.19074940614627778 2216 86-87 0.06741870523870475 2216 88-89 0.05180930076587842 2216 90-91 0.20123688504942194 2216 92-93 0.3273398128065068 2216 94-95 0.32437875185272347 2216 96-97 0.27606660396353533 2216 98-99 0.30339659686438836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 6.0 7 209.0 8 1286.0 9 2446.0 10 4207.0 11 8141.0 12 15949.0 13 27501.0 14 36714.0 15 39873.0 16 42749.0 17 45599.0 18 48092.0 19 52230.0 20 56878.0 21 62890.0 22 70002.0 23 78331.0 24 88960.0 25 100035.0 26 110717.0 27 123453.0 28 144851.0 29 175823.0 30 217190.0 31 270182.0 32 348347.0 33 450788.0 34 598708.0 35 841555.0 36 1236457.0 37 1405749.0 38 383762.0 39 245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.868723249251914 18.5046450829401 12.731469564180992 24.895162103626994 2 16.006998100402768 20.52855573628152 37.80772544247301 25.656720720842706 3 18.058995886835362 25.83407875803359 28.894904003121052 27.212021352009998 4 13.146288571458795 17.213002958423395 36.104838908733164 33.535869561384644 5 14.64568384009704 37.33699580743097 32.985807325183266 15.031513027288723 6 32.04559991819377 36.57298208373149 17.28596564843775 14.095452349636986 7 28.125854645853092 32.030860693166716 21.508506789564063 18.33477787141613 8 26.98737997933688 33.54715317863024 19.667119186733288 19.798347655299597 9 26.95658980877795 15.817346445836874 19.05684474800509 38.169218997380085 10-11 25.460332796186137 25.787924413868975 26.649781203609347 22.101961586335538 12-13 26.11759644848147 23.781640849515334 26.179832649851726 23.92093005215147 14-15 24.16971265563458 24.653638790255187 23.51594692468538 27.660701629424857 16-17 24.171117760139758 27.04304538080765 24.25001845929389 24.5358183997587 18-19 24.542234226737236 26.626917774165452 25.412222273155216 23.418625725942093 20-21 25.974677623950875 25.87252520153506 24.424471418513853 23.728325756000213 22-23 25.46316075275832 25.576067447822087 24.38226074323776 24.578511056181835 24-25 24.70639271006907 26.050379749138685 24.455765542682187 24.787461998110054 26-27 24.820609866861066 26.078514658371894 24.373270829611794 24.72760464515525 28-29 24.558035952949513 26.002186951331446 24.758252588647064 24.681524507071977 30-31 24.843125055801345 25.987593871436243 24.61847395789183 24.550807114870583 32-33 24.576776080642908 26.229466808031628 24.244501668071713 24.949255443253758 34-35 24.85772233162243 26.220114002672666 24.594757501898997 24.327406163805904 36-37 24.83319640193655 26.037193623345807 24.4254135269414 24.704196447776248 38-39 24.84164146188309 25.945849349019824 24.498979114585715 24.71353007451138 40-41 24.74364679457117 26.052532290539038 24.760734422437473 24.443086492452316 42-43 24.87604593842671 26.003596652997036 24.66221433936184 24.458143069214415 44-45 24.5054778435597 26.22753414175749 24.52629611737783 24.740691897304977 46-47 24.248606296963647 26.24137067740491 24.852251610560057 24.657771415071387 48-49 24.761654238206127 26.12750373417998 24.668472519397284 24.442369508216608 50-51 24.156623659629687 26.369848764267605 24.618751256183952 24.85477631991876 52-53 24.289925492864874 26.152850700113188 24.521056287619402 25.03616751940253 54-55 24.452416633462274 25.916790375074488 24.669435854399026 24.961357137064212 56-57 24.483044602023295 25.71276847075251 24.51947658120502 25.284710346019175 58-59 24.27267566300067 25.698388911025148 24.95532040183782 25.073615024136366 60-61 24.414722299601195 25.809962728801782 24.72749006512763 25.04782490646939 62-63 24.10406316004753 25.84937781429282 24.56884240665451 25.477716619005136 64-65 23.98980243091429 25.6420554519265 24.749020617284387 25.619121499874826 66-67 24.452996489030735 25.54757121816212 24.665890212481166 25.333542080325984 68-69 24.28028505238067 25.341121379986387 24.78330870918945 25.595284858443495 70-71 23.941155936064202 25.329435783876413 24.87474127018269 25.854667009876692 72-73 24.35001075469769 25.450889819003724 24.859388498467897 25.339710927830687 74-75 24.4321695927672 25.30624089817593 24.90213225104638 25.359457258010487 76-77 24.196899120935694 25.397102226046115 24.954720959671647 25.451277693346547 78-79 24.278564300750713 25.391178326992176 24.94408615041767 25.386171221839444 80-81 24.04938698223183 25.27277792078195 25.239293786605643 25.438541310380575 82-83 24.24522826405075 25.26467487314746 25.0403777190873 25.449719143714493 84-85 24.292584195178367 25.35567724623321 24.787779842523015 25.563958716065404 86-87 24.112702179501195 25.135597344118594 25.06742313917284 25.684277337207373 88-89 24.26964319086591 24.798717898990468 25.14898253507618 25.782656375067436 90-91 24.41389718509011 24.94761228080692 24.970948211722973 25.66754232238 92-93 24.30127963271826 24.924163513718412 25.047895429077176 25.726661424486153 94-95 23.938525442793825 24.93610299121641 25.35769419281586 25.767677373173903 96-97 24.375208482459264 24.875742691213233 25.00250002644598 25.746548799881523 98-99 24.206892174458826 24.92778132349778 24.90977690173027 25.95554960031312 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1124.0 1 1101.5 2 1114.0 3 1121.5 4 1007.0 5 861.5 6 750.5 7 683.0 8 700.0 9 674.0 10 622.5 11 644.5 12 639.5 13 667.5 14 710.5 15 841.5 16 1017.5 17 1156.0 18 1422.0 19 1826.5 20 2538.5 21 3602.5 22 5246.0 23 7283.0 24 9681.0 25 13120.5 26 18218.0 27 23470.0 28 29630.5 29 37921.5 30 45331.0 31 54280.0 32 64541.5 33 74183.0 34 83797.5 35 93446.5 36 102202.5 37 113652.5 38 129482.5 39 137616.0 40 144089.5 41 155703.0 42 169470.0 43 189896.5 44 212110.0 45 235855.5 46 264720.0 47 288309.0 48 302417.5 49 311442.0 50 322975.5 51 323274.0 52 316753.5 53 316417.0 54 306035.5 55 294838.5 56 273288.5 57 250509.0 58 235452.0 59 208589.0 60 177199.0 61 143522.5 62 117476.0 63 95037.0 64 73267.5 65 57555.5 66 44110.0 67 34624.0 68 27863.0 69 23818.0 70 20537.5 71 17403.5 72 15538.5 73 13430.5 74 10403.5 75 8036.0 76 6027.0 77 4322.0 78 3006.0 79 2091.5 80 1490.0 81 1092.5 82 795.0 83 539.0 84 368.5 85 259.5 86 172.5 87 114.5 88 86.0 89 58.0 90 45.5 91 35.0 92 21.0 93 15.5 94 11.0 95 8.0 96 7.5 97 4.5 98 5.5 99 9.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002397768664689683 2 1.4104521556998135E-5 3 9.873165089898694E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.052260778499068E-6 18-19 0.0 20-21 4.1608338593144504E-4 22-23 0.0 24-25 0.004379453943447921 26-27 7.052260778499068E-6 28-29 0.008519131020426873 30-31 5.923899053939216E-4 32-33 2.820904311399627E-5 34-35 4.936582544949347E-5 36-37 0.0 38-39 1.4104521556998135E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.1156782335497203E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.5261303892495336E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7089925.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.78969562853706 #Duplication Level Percentage of deduplicated Percentage of total 1 75.01792134747436 33.60029863838909 2 13.253531443054825 11.872432786753425 3 4.616649663985543 6.20334999820501 4 2.120795680558007 3.79959172090037 5 1.2072618567661693 2.703644555424961 6 0.7291266898529858 1.9594417507894781 7 0.516321085511938 1.6188104986672895 8 0.3775666479662302 1.3528876193515562 9 0.2906099411777528 1.1714697730780745 >10 1.610110342677367 13.374297324078643 >50 0.13603814548899965 4.224586874435821 >100 0.10553383373041042 9.676849285681504 >500 0.012524325799301295 3.849871266478427 >1k 0.005945743367167487 4.341472584478978 >5k 6.325258892159317E-5 0.2509953232876132 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9184 0.12953592597947086 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8535 0.12038209148897909 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.4104521556998135E-5 0.0 2 0.0 0.0 0.0 1.4104521556998135E-5 0.0 3 0.0 0.0 0.0 1.4104521556998135E-5 0.0 4 0.0 0.0 0.0 1.4104521556998135E-5 1.4104521556998135E-5 5 0.0 0.0 0.0 2.820904311399627E-5 1.4104521556998135E-5 6 0.0 0.0 0.0 2.820904311399627E-5 1.4104521556998135E-5 7 0.0 0.0 0.0 2.820904311399627E-5 1.4104521556998135E-5 8 0.0 0.0 0.0 2.820904311399627E-5 1.4104521556998135E-5 9 1.4104521556998135E-5 0.0 0.0 2.820904311399627E-5 1.4104521556998135E-5 10-11 2.1156782335497202E-5 0.0 0.0 3.5261303892495336E-5 1.4104521556998135E-5 12-13 4.2313564670994404E-5 0.0 0.0 4.2313564670994404E-5 2.1156782335497202E-5 14-15 4.936582544949347E-5 0.0 0.0 5.6418086227992534E-5 5.641808622799254E-5 16-17 9.167939012048788E-5 0.0 0.0 7.052260778499067E-5 9.873165089898694E-5 18-19 9.873165089898694E-5 0.0 0.0 7.757486856348974E-5 1.4104521556998134E-4 20-21 1.269406940129832E-4 0.0 0.0 2.3272460569046923E-4 1.480974763484804E-4 22-23 1.4104521556998134E-4 0.0 0.0 9.873165089898694E-4 1.6925425868397762E-4 24-25 1.974633017979739E-4 0.0 0.0 0.0030254198739761 1.8335878024097575E-4 26-27 2.4682912724746734E-4 0.0 0.0 0.004929530284170848 1.8335878024097575E-4 28-29 2.538813880259664E-4 0.0 0.0 0.014993106415089018 1.9041104101947482E-4 30-31 2.6798590958296455E-4 0.0 0.0 0.046016001579706416 1.974633017979739E-4 32-33 2.6798590958296455E-4 0.0 0.0 0.09935224984749486 2.1156782335497203E-4 34-35 2.961949526969608E-4 0.0 0.0 0.1687182868648117 2.1156782335497203E-4 36-37 3.244039958109571E-4 0.0 0.0 0.27706922146567137 2.1156782335497203E-4 38-39 3.244039958109571E-4 0.0 0.0 0.43940521232594143 2.2567234491197016E-4 40-41 3.3850851736795523E-4 0.0 0.0 0.6307259949858426 2.2567234491197016E-4 42-43 3.455607781464543E-4 0.0 0.0 0.8091552449426476 2.3272460569046923E-4 44-45 3.8082208203894964E-4 0.0 0.0 1.0174860806002883 2.397768664689683E-4 46-47 3.8082208203894964E-4 0.0 0.0 1.2436943973314245 2.397768664689683E-4 48-49 3.949266035959478E-4 0.0 0.0 1.4833372708456014 2.538813880259664E-4 50-51 3.949266035959478E-4 0.0 0.0 1.7511116126052109 2.538813880259664E-4 52-53 3.949266035959478E-4 0.0 0.0 2.005881585489268 2.8914269191846175E-4 54-55 3.949266035959478E-4 1.4104521556998135E-5 0.0 2.26063040159099 3.244039958109571E-4 56-57 3.949266035959478E-4 1.4104521556998135E-5 0.0 2.550386640197181 3.244039958109571E-4 58-59 3.949266035959478E-4 3.5261303892495336E-5 0.0 2.862999255986488 3.244039958109571E-4 60-61 3.949266035959478E-4 5.641808622799254E-5 0.0 3.180626029189307 3.244039958109571E-4 62-63 3.949266035959478E-4 5.641808622799254E-5 0.0 3.4979566074394297 3.5261303892495337E-4 64-65 3.949266035959478E-4 6.34703470064916E-5 0.0 3.8324171271205265 3.5261303892495337E-4 66-67 3.949266035959478E-4 8.462712934198881E-5 0.0 4.193210788548539 3.5261303892495337E-4 68-69 3.949266035959478E-4 8.462712934198881E-5 0.0 4.573990557022817 3.5261303892495337E-4 70-71 3.949266035959478E-4 8.462712934198881E-5 0.0 4.994672016981844 3.5261303892495337E-4 72-73 3.949266035959478E-4 8.462712934198881E-5 0.0 5.411185590820777 3.8082208203894964E-4 74-75 3.949266035959478E-4 8.462712934198881E-5 0.0 5.827360656142343 3.8082208203894964E-4 76-77 3.949266035959478E-4 8.462712934198881E-5 0.0 6.270884388762928 3.8082208203894964E-4 78-79 3.949266035959478E-4 8.462712934198881E-5 0.0 6.732236236631558 3.8082208203894964E-4 80-81 3.949266035959478E-4 8.462712934198881E-5 0.0 7.2295193531666415 3.949266035959478E-4 82-83 4.090311251529459E-4 8.462712934198881E-5 0.0 7.7536363219639135 4.2313564670994405E-4 84-85 4.090311251529459E-4 8.462712934198881E-5 0.0 8.270729239025801 4.2313564670994405E-4 86-87 4.090311251529459E-4 9.873165089898694E-5 0.0 8.802575485636307 4.2313564670994405E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 24025 0.0 26.10949 1 CAACGCA 27010 0.0 20.865454 5 ATCAACG 27470 0.0 20.65147 3 AACGCAG 27735 0.0 20.521215 6 TATCAAC 30305 0.0 20.484097 2 GTACATG 38495 0.0 20.063889 1 TCAACGC 29065 0.0 19.454187 4 TACATGG 38105 0.0 19.3052 2 ACGCAGA 29295 0.0 18.888752 7 CGCAGAG 29555 0.0 18.565252 8 GTACTAG 2180 0.0 18.343904 1 CATGGGG 17445 0.0 17.778893 4 ACATGGG 38685 0.0 17.705572 3 GCAGAGT 30095 0.0 17.598644 9 GTACATA 1985 0.0 17.569143 1 GTACAAA 4445 0.0 17.365454 1 GTACAAG 4535 0.0 16.815754 1 TAGGCAT 3280 0.0 16.303236 5 GTATAAA 10225 0.0 16.189615 1 GTATATA 2020 0.0 15.653352 1 >>END_MODULE