FastQCFastQC Report
Fri 27 May 2016
ERR765421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7089925
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228360.3220908542756094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139150.19626441746562903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118210.16672954932527495No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94540.13334414679986037No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89640.12643293123693128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA348750.037.2905461
GGTATCA210100.028.2589341
ATCAACG457050.028.0912043
CAACGCA456700.028.0821885
TATCAAC470550.027.601512
TATCACG7450.027.4640182
AACGCAG478500.027.1899836
TCAACGC476100.026.9965064
ACGCAGA520200.024.7064787
CGCAGAG530000.024.2320968
AGTACTT316450.023.23438512-13
GCAGAGT561400.022.8356659
AGAGTAC534550.020.82300610-11
TACTTTT360300.020.5034714-15
GTACTAG20300.020.1705511
GAGTACT305300.019.71876312-13
GTACTTT335900.017.93625814-15
CAGAGTA547150.017.421959
TAGTACT30750.017.0883854
CATGGGG145750.016.8458544
Produced by FastQC (version 0.11.5)