##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765421_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7089925 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.383586850354554 33.0 33.0 33.0 33.0 33.0 2 32.27836331131853 33.0 33.0 33.0 33.0 33.0 3 32.32620754098245 33.0 33.0 33.0 33.0 33.0 4 36.24496902294453 37.0 37.0 37.0 33.0 37.0 5 36.181628719626794 37.0 37.0 37.0 33.0 37.0 6 36.20469285641244 37.0 37.0 37.0 33.0 37.0 7 36.10513242382677 37.0 37.0 37.0 33.0 37.0 8 36.11404789190295 37.0 37.0 37.0 33.0 37.0 9 36.13141084003004 37.0 37.0 37.0 33.0 37.0 10-11 36.09044707807205 37.0 37.0 37.0 33.0 37.0 12-13 36.066165015849954 37.0 37.0 37.0 33.0 37.0 14-15 37.55537942926053 40.0 37.0 40.0 33.0 40.0 16-17 37.55897199194632 40.0 37.0 40.0 33.0 40.0 18-19 37.6177480015656 40.0 37.0 40.0 33.0 40.0 20-21 37.606135043741645 40.0 37.0 40.0 33.0 40.0 22-23 37.57400614251914 40.0 37.0 40.0 33.0 40.0 24-25 37.547965740117135 40.0 37.0 40.0 33.0 40.0 26-27 37.42853683219498 40.0 37.0 40.0 33.0 40.0 28-29 37.33345818185665 38.5 37.0 40.0 33.0 40.0 30-31 37.18789662796151 37.0 37.0 40.0 33.0 40.0 32-33 37.08061333511991 37.0 37.0 40.0 33.0 40.0 34-35 36.98498333903391 37.0 37.0 40.0 33.0 40.0 36-37 36.83629904406605 37.0 37.0 40.0 33.0 40.0 38-39 36.72595330698138 37.0 37.0 40.0 33.0 40.0 40-41 36.56669908355872 37.0 37.0 40.0 33.0 40.0 42-43 36.414923641646425 37.0 37.0 40.0 33.0 40.0 44-45 36.131114362986914 37.0 37.0 40.0 33.0 40.0 46-47 35.8875849180351 37.0 37.0 40.0 30.0 40.0 48-49 35.609271466200276 37.0 37.0 40.0 27.0 40.0 50-51 35.40645761414966 37.0 37.0 40.0 27.0 40.0 52-53 35.252871715850304 37.0 33.0 40.0 27.0 40.0 54-55 35.06492536945031 37.0 33.0 40.0 27.0 40.0 56-57 34.86850213507195 37.0 33.0 40.0 27.0 40.0 58-59 34.65670144606607 37.0 33.0 40.0 27.0 40.0 60-61 34.4957052437085 37.0 33.0 37.0 27.0 40.0 62-63 34.27676343543831 37.0 33.0 37.0 27.0 40.0 64-65 34.050963726696686 37.0 33.0 37.0 27.0 40.0 66-67 33.82223852861631 37.0 33.0 37.0 27.0 40.0 68-69 33.61006258881441 37.0 33.0 37.0 27.0 40.0 70-71 33.42278190530929 37.0 33.0 37.0 27.0 38.5 72-73 33.213866648800945 37.0 33.0 37.0 27.0 37.0 74-75 32.98596169917171 37.0 33.0 37.0 27.0 37.0 76-77 32.81140371724666 37.0 33.0 37.0 27.0 37.0 78-79 32.64525245330522 35.0 33.0 37.0 24.5 37.0 80-81 32.47487053812276 33.0 33.0 37.0 22.0 37.0 82-83 32.376679725102875 33.0 33.0 37.0 22.0 37.0 84-85 32.250073801909046 33.0 33.0 37.0 22.0 37.0 86-87 32.143027465029604 33.0 33.0 37.0 22.0 37.0 88-89 32.0315355240006 33.0 33.0 37.0 22.0 37.0 90-91 31.92943881634855 33.0 33.0 37.0 22.0 37.0 92-93 31.854139923906107 33.0 33.0 37.0 22.0 37.0 94-95 31.754013265302525 33.0 33.0 37.0 22.0 37.0 96-97 31.69728607848461 33.0 33.0 37.0 22.0 37.0 98-99 30.911045180308676 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.021802566036257076 1101 2 0.09477325111094359 1101 3 0.06837759561216927 1101 4 0.08945678255936684 1101 5 0.02470577803506302 1101 6 0.038556364980593116 1101 7 0.05595583880585764 1101 8 0.10701610209430612 1101 9 0.07059507826094347 1101 10-11 0.04801424857856773 1101 12-13 0.04266200385258401 1101 14-15 0.07841136548947958 1101 16-17 0.09673230201931204 1101 18-19 0.09230993731488013 1101 20-21 0.13643694743672796 1101 22-23 0.046291092016502944 1101 24-25 0.10164570624548475 1101 26-27 0.07783361209704509 1101 28-29 0.053401400428214174 1101 30-31 0.05198155518763059 1101 32-33 0.14376851090715803 1101 34-35 0.1766988862229013 1101 36-37 0.1522992882751808 1101 38-39 0.11705261438002879 1101 40-41 0.0728836771984831 1101 42-43 0.10417925972178921 1101 44-45 0.19236110360543535 1101 46-47 0.22313321412739384 1101 48-49 0.14212836128832862 1101 50-51 0.1973320647095207 1101 52-53 0.23217190983962865 1101 54-55 0.0995341178634277 1101 56-57 0.1366413385115237 1101 58-59 0.1774794735401528 1101 60-61 0.04908966250990687 1101 62-63 0.1277167794624532 1101 64-65 0.08212725906107465 1101 66-67 0.2373741530023068 1101 68-69 0.2365728990473812 1101 70-71 0.09460702529376164 1101 72-73 0.11131748586896606 1101 74-75 0.10771925308598185 1101 76-77 0.18082030233134105 1101 78-79 0.11034106406287947 1101 80-81 0.20255497532097166 1101 82-83 0.18690950158806885 1101 84-85 0.14650771616368274 1101 86-87 0.1267923757732916 1101 88-89 0.1059408553006449 1101 90-91 0.1674299326641986 1101 92-93 0.13595655002502482 1101 94-95 0.19531457636541916 1101 96-97 0.11322111355243791 1101 98-99 0.1868617125702947 1102 1 0.01335451421041256 1102 2 0.13360814231833018 1102 3 0.0904675385978635 1102 4 0.08548517335950123 1102 5 0.06776942462389712 1102 6 0.09036511493211208 1102 7 0.05080942450185688 1102 8 0.161438201927524 1102 9 0.08521459499677775 1102 10-11 0.02740929570264683 1102 12-13 0.04458284316531547 1102 14-15 0.09577949565276356 1102 16-17 0.10751876984694775 1102 18-19 0.14434335080439098 1102 20-21 0.17753631523721936 1102 22-23 0.12230552012805163 1102 24-25 0.11973931443693431 1102 26-27 0.11564922703712455 1102 28-29 0.10336746333788227 1102 30-31 0.1757108535625278 1102 32-33 0.1463692555838776 1102 34-35 0.19012216900613055 1102 36-37 0.2032619380831946 1102 38-39 0.1709572220672655 1102 40-41 0.17004634252059958 1102 42-43 0.19262746202563363 1102 44-45 0.22349586294298263 1102 46-47 0.28536540200313 1102 48-49 0.2508023906875394 1102 50-51 0.30366860428986797 1102 52-53 0.28209589075728303 1102 54-55 0.21266030566158634 1102 56-57 0.2601702141272426 1102 58-59 0.3116200894498533 1102 60-61 0.2744672368806178 1102 62-63 0.31984405324750753 1102 64-65 0.23938572394103375 1102 66-67 0.29713329662406807 1102 68-69 0.23208612481629842 1102 70-71 0.17442890405108358 1102 72-73 0.13333732452234415 1102 74-75 0.37568215499809554 1102 76-77 0.42065245917887495 1102 78-79 0.34003340009824967 1102 80-81 0.30718661244620904 1102 82-83 0.31183367643196647 1102 84-85 0.21857332140745456 1102 86-87 0.21867161790958534 1102 88-89 0.3287332960561855 1102 90-91 0.25765023286199806 1102 92-93 0.23729037463813896 1102 94-95 0.23030546183258593 1102 96-97 0.2771222305675245 1102 98-99 0.2527358350541 1103 1 0.00903675546715732 1103 2 0.14645106623854787 1103 3 0.08668857933228935 1103 4 0.1074997269854947 1103 5 0.059995368981006436 1103 6 0.05910275655916308 1103 7 0.0652495533405073 1103 8 0.09995674047491576 1103 9 0.010131579094114329 1103 10-11 0.06738433664589394 1103 12-13 0.10856284763298163 1103 14-15 0.18787899326855495 1103 16-17 0.12830753011812135 1103 18-19 0.16854034099173276 1103 20-21 0.11889935428524723 1103 22-23 0.10818209676046564 1103 24-25 0.11469696931724371 1103 26-27 0.15469881527508988 1103 28-29 0.14916708287865532 1103 30-31 0.1473771451500383 1103 32-33 0.14362863883727073 1103 34-35 0.19228012073281775 1103 36-37 0.14498833107934672 1103 38-39 0.09660382179097127 1103 40-41 0.09404756218651045 1103 42-43 0.052270127370022124 1103 44-45 0.09521012167771659 1103 46-47 0.08687004625961237 1103 48-49 0.13508156688891404 1103 50-51 0.16552440583541284 1103 52-53 0.03069832501408598 1103 54-55 0.010410567003710014 1103 56-57 0.09846610956876134 1103 58-59 0.13219190975961936 1103 60-61 0.03300760628297894 1103 62-63 0.15000140762077052 1103 64-65 0.09439790604064768 1103 66-67 0.15182227973801332 1103 68-69 0.17936705605534797 1103 70-71 0.061425822023558396 1103 72-73 0.08041696928840736 1103 74-75 0.12491932302704356 1103 76-77 0.16435001864071097 1103 78-79 0.19229686207749808 1103 80-81 0.10904522188564414 1103 82-83 0.06320907437292078 1103 84-85 0.005526423755810583 1103 86-87 0.046529610860311266 1103 88-89 0.17646569087351338 1103 90-91 0.1098565926792503 1103 92-93 0.10948049026929141 1103 94-95 0.023072127514758733 1103 96-97 0.06543003563895766 1103 98-99 0.028708674608548534 1104 1 0.007940158836433397 1104 2 0.04649922502167669 1104 3 -0.04739628782456151 1104 4 -0.04276591189974255 1104 5 -0.04193588805456727 1104 6 0.05503170971407911 1104 7 0.07083202824544799 1104 8 0.11216571499656425 1104 9 0.03831653947783309 1104 10-11 0.0033700147093540522 1104 12-13 -0.0433213584952199 1104 14-15 0.004251187059750805 1104 16-17 0.05282553779117194 1104 18-19 0.01972292197223169 1104 20-21 0.025466714876202445 1104 22-23 0.07151954083883538 1104 24-25 0.015041090388308476 1104 26-27 0.024431540206798275 1104 28-29 0.010259608337300108 1104 30-31 -0.07422664668707313 1104 32-33 -0.023929425331928655 1104 34-35 -0.05438627944255359 1104 36-37 -0.01440228849737224 1104 38-39 -0.11361919735597326 1104 40-41 -0.03729284542193767 1104 42-43 0.016848930789734595 1104 44-45 -0.04808779686668885 1104 46-47 -0.06828270643094925 1104 48-49 -0.04391632049971861 1104 50-51 -0.13659760870449134 1104 52-53 -0.05824891617240269 1104 54-55 -0.1655260434344612 1104 56-57 -0.1092414096282539 1104 58-59 -0.07934648017246815 1104 60-61 -0.13458672057162602 1104 62-63 -0.13064719878423858 1104 64-65 -0.11156670835755733 1104 66-67 -0.04978999841069509 1104 68-69 -0.07788611970348569 1104 70-71 -0.21210277912909703 1104 72-73 -0.12502665964704818 1104 74-75 -0.0828679306645057 1104 76-77 -0.0919958928644462 1104 78-79 0.005478665891921253 1104 80-81 -0.060262638954547754 1104 82-83 -0.06528238939844755 1104 84-85 0.012730054008358138 1104 86-87 -0.07268272971682421 1104 88-89 -0.055560456764293775 1104 90-91 -0.13929826175263926 1104 92-93 -0.05240966649587975 1104 94-95 -0.06943271812675889 1104 96-97 -0.054440343064367624 1104 98-99 -0.06213167745583448 1105 1 -0.021587109720165643 1105 2 0.09891294115471538 1105 3 0.05580330873721806 1105 4 0.02199391959014463 1105 5 0.09594834135999974 1105 6 0.058704679800186454 1105 7 0.03671863087875238 1105 8 0.10818079761787658 1105 9 0.03189024604213131 1105 10-11 0.0389816949472106 1105 12-13 0.017734377871505558 1105 14-15 0.08862811762200806 1105 16-17 0.07177817145839782 1105 18-19 0.08134904341420679 1105 20-21 0.06192353341521084 1105 22-23 0.0984889477939177 1105 24-25 0.09331426968211787 1105 26-27 0.06381559733642206 1105 28-29 -0.0010658314742357788 1105 30-31 0.028883810224407114 1105 32-33 0.07781911727336421 1105 34-35 0.028841791695299435 1105 36-37 0.060377311767545905 1105 38-39 0.02206844157885257 1105 40-41 0.0013316205895321787 1105 42-43 0.00668963241037801 1105 44-45 0.07446679799563327 1105 46-47 -0.006907497672713703 1105 48-49 -0.026805344862509628 1105 50-51 -0.06065918883737709 1105 52-53 -0.10146487895318046 1105 54-55 -0.050238595067980896 1105 56-57 -0.012527743118553758 1105 58-59 0.028281790593730705 1105 60-61 -0.052543864959041287 1105 62-63 -0.05847543938864419 1105 64-65 0.058330888984549745 1105 66-67 0.052668790741819294 1105 68-69 -0.03295078382434724 1105 70-71 -0.12145345460462664 1105 72-73 -0.0753323873250693 1105 74-75 -5.60949887429274E-4 1105 76-77 0.021617001129548896 1105 78-79 0.033389249497190576 1105 80-81 0.013168860995051546 1105 82-83 0.0314442780263704 1105 84-85 0.011898426868832246 1105 86-87 0.06363004001016037 1105 88-89 0.06786133730109611 1105 90-91 0.032323338241525335 1105 92-93 0.015170932146400418 1105 94-95 0.025118930215462854 1105 96-97 -0.01456117673879831 1105 98-99 0.06728579932672574 1106 1 -0.03306645155644361 1106 2 0.07333562999928489 1106 3 -0.027120726349409097 1106 4 0.07601119685538293 1106 5 0.027587848548002114 1106 6 0.04401968441760573 1106 7 -0.029270227561724482 1106 8 -0.0068993453051646725 1106 9 -0.012342979122806241 1106 10-11 -0.02590306796658126 1106 12-13 -0.03153220030100101 1106 14-15 0.04932146211763211 1106 16-17 0.015082060158917443 1106 18-19 0.0282304768975834 1106 20-21 0.019070366720825405 1106 22-23 0.010102151300259266 1106 24-25 0.05496444740479234 1106 26-27 0.0019795406208089616 1106 28-29 -0.02459511237799461 1106 30-31 0.033731420739727014 1106 32-33 -0.00742788319114851 1106 34-35 0.10362824511074109 1106 36-37 0.0918522040065497 1106 38-39 0.0011822160927437153 1106 40-41 0.006740106724883788 1106 42-43 -0.07850880874856614 1106 44-45 -0.008992051643069487 1106 46-47 0.0864335992453178 1106 48-49 0.02905026347971784 1106 50-51 -0.020029037333074484 1106 52-53 0.057087514075639945 1106 54-55 -0.059939178625469935 1106 56-57 0.10204727008849801 1106 58-59 0.10026049401712811 1106 60-61 -0.010340469954662979 1106 62-63 0.045964471210879765 1106 64-65 0.0032165402902890605 1106 66-67 -0.04999000125459929 1106 68-69 -0.03020249287992982 1106 70-71 -0.057769475201510545 1106 72-73 0.005141696123011741 1106 74-75 -0.03275092695588455 1106 76-77 0.08243384868088555 1106 78-79 -0.03889053543331045 1106 80-81 0.014888046475647343 1106 82-83 -0.05436881839681007 1106 84-85 -0.030762676051651283 1106 86-87 0.0604600553924044 1106 88-89 0.09749372625472574 1106 90-91 -0.022548831427975813 1106 92-93 0.06868694315248192 1106 94-95 -0.033678771603781854 1106 96-97 -0.04480394718526881 1106 98-99 0.0686006340332952 1107 1 -0.024008112254215064 1107 2 0.026601780565563615 1107 3 0.05198212260053481 1107 4 -0.033193890064595166 1107 5 -0.02102303077334966 1107 6 0.030782218719984655 1107 7 1.4344738161753412E-5 1107 8 -0.038561363683271566 1107 9 -0.00520886890465988 1107 10-11 -0.05748456580424488 1107 12-13 -0.08607554743089452 1107 14-15 -0.06750565872751224 1107 16-17 -0.08628639758139656 1107 18-19 -0.059058629682525066 1107 20-21 -0.051897728909835905 1107 22-23 -0.0406650668055164 1107 24-25 -0.06718115299862859 1107 26-27 -0.04469581322174321 1107 28-29 -0.059611942516042404 1107 30-31 -0.07165496525291815 1107 32-33 0.008270605796653285 1107 34-35 -0.0646680087164242 1107 36-37 0.010439248378055765 1107 38-39 -0.08073915381544339 1107 40-41 -0.14776530304403934 1107 42-43 -0.04595525715240001 1107 44-45 -0.05610184348204683 1107 46-47 -0.052492362580878194 1107 48-49 -0.09848755918558538 1107 50-51 -0.0020007484866226832 1107 52-53 -0.08279972060044116 1107 54-55 -0.1553849265481233 1107 56-57 -0.13433665577893095 1107 58-59 -0.08750289924618926 1107 60-61 -0.025225928620024263 1107 62-63 -0.09608449874478708 1107 64-65 -0.12464532177310161 1107 66-67 -0.07984297752960856 1107 68-69 -0.1254314023780978 1107 70-71 -0.055199414518483536 1107 72-73 -0.07366974208837007 1107 74-75 -0.009930751770511392 1107 76-77 -0.013530417854560994 1107 78-79 -0.001665942744651261 1107 80-81 -0.10209469881905875 1107 82-83 -0.08276882056829749 1107 84-85 -0.0651395342179697 1107 86-87 -0.054876932050092364 1107 88-89 -0.06118154726864944 1107 90-91 -0.08688439287482197 1107 92-93 0.08035900239446292 1107 94-95 -0.048724510202582394 1107 96-97 -0.046918302042808335 1107 98-99 -0.0019476263557471896 1108 1 -0.06408528195566987 1108 2 0.014780425355411353 1108 3 -0.008412651314408492 1108 4 -0.02416531411358136 1108 5 0.03249704651166496 1108 6 0.01841145693958879 1108 7 -0.06573630812579268 1108 8 -0.03046084479679223 1108 9 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-0.1508410588155371 2215 60-61 -0.15511056850845506 2215 62-63 -0.17498562136785267 2215 64-65 -0.060230564641990725 2215 66-67 -0.14174002194644686 2215 68-69 -0.0740479333864954 2215 70-71 -0.1768266414427302 2215 72-73 -0.1448666844847537 2215 74-75 -0.2359092532166045 2215 76-77 -0.0834791815561644 2215 78-79 -0.06653537316401525 2215 80-81 -0.11549148408005294 2215 82-83 -0.14019809316317833 2215 84-85 -0.050366640439150956 2215 86-87 -0.15296968931901844 2215 88-89 -0.08815306587338867 2215 90-91 -0.14237716431118486 2215 92-93 -0.047843744358967655 2215 94-95 0.04899769681013311 2215 96-97 -0.0611515210332918 2215 98-99 -0.18145566621960185 2216 1 -0.025517205167979284 2216 2 -0.04375322457402575 2216 3 -0.04201594030732991 2216 4 -0.14565469132869424 2216 5 -0.08929420602333238 2216 6 -0.11967653624034824 2216 7 -0.07873394483303287 2216 8 -0.13716207273487413 2216 9 -0.06590908303571297 2216 10-11 -0.06613130147102453 2216 12-13 -0.05216265683635868 2216 14-15 -0.21106334233158464 2216 16-17 -0.17502787433912914 2216 18-19 -0.11524376375332679 2216 20-21 -0.1775603970840649 2216 22-23 -0.18945235264679638 2216 24-25 -0.29284616790085494 2216 26-27 -0.2290111311554952 2216 28-29 -0.15693358488558573 2216 30-31 -0.17864672577025686 2216 32-33 -0.1119944188891111 2216 34-35 -0.13137016993222517 2216 36-37 -0.1405955541284527 2216 38-39 -0.17570944852820247 2216 40-41 -0.11373347338761874 2216 42-43 -0.1688121249160588 2216 44-45 -0.21588347310161993 2216 46-47 -0.2382149448038291 2216 48-49 -0.10358698835187852 2216 50-51 -0.09014640384373251 2216 52-53 -0.11242238042145658 2216 54-55 -0.08024736970088497 2216 56-57 -0.10530080478541493 2216 58-59 -0.22497893310400485 2216 60-61 -0.2363080391028447 2216 62-63 -0.16189203236733363 2216 64-65 -0.18584605462866222 2216 66-67 -0.16841009078330416 2216 68-69 -0.04733811741149907 2216 70-71 -0.14546664880276694 2216 72-73 -0.11903018013770605 2216 74-75 -0.14957869276227598 2216 76-77 -0.11395478360308431 2216 78-79 -0.04363334494797044 2216 80-81 -0.16230460206960373 2216 82-83 -0.18870685179125957 2216 84-85 -0.23019044829965196 2216 86-87 -0.24228563026465366 2216 88-89 -0.22598428314302055 2216 90-91 -0.14657994531103924 2216 92-93 -0.04534019264324485 2216 94-95 -0.11708219462450131 2216 96-97 -0.13323479323294762 2216 98-99 -0.1307781582508305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 15.0 10 52.0 11 122.0 12 282.0 13 486.0 14 1057.0 15 2787.0 16 6237.0 17 12471.0 18 20745.0 19 30799.0 20 40719.0 21 53364.0 22 65985.0 23 65333.0 24 55513.0 25 51389.0 26 58511.0 27 74285.0 28 98233.0 29 129228.0 30 171983.0 31 226474.0 32 298833.0 33 396389.0 34 527702.0 35 752288.0 36 1257849.0 37 1959040.0 38 731038.0 39 713.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.966374235168665 18.587708845032004 13.398667998141656 25.047248921657676 2 16.159183630292283 20.825974886899367 37.18638772624534 25.828453756563007 3 18.139895697063086 25.605418957182195 29.77457448421528 26.480110861539437 4 13.44134105790964 17.071111471559995 36.207971170357936 33.27957630017242 5 14.360813190421675 36.85456028824567 33.14005710974584 15.644569411586811 6 31.84786863048622 36.80394362422734 16.966089204046586 14.382098541239857 7 27.36823027041894 31.96465406897816 22.020500922083098 18.6466147385198 8 27.380063964005263 32.350046016001585 19.898433340268056 20.371456679725103 9 27.33241889018572 14.131813806210927 19.748093244992013 38.78767405861134 10-11 25.651195181895435 24.866200982379926 27.164899487653255 22.31770434807138 12-13 26.815241345994494 22.851095039792384 27.116020268197477 23.21764334601565 14-15 24.208336965300397 23.386990533753877 25.48025183142821 26.924420669517513 16-17 23.462009303498608 25.93407715621395 25.995427662893388 24.608485877394056 18-19 23.73900915967419 24.69695894754792 28.675636445802123 22.888395446975768 20-21 24.820434956156 24.085051855383213 28.03613868159116 23.058374506869622 22-23 24.370913655645158 23.76033596970349 27.990817956466397 23.87793241818496 24-25 23.888390920919473 24.256138111475085 28.030472818823892 23.824998148781546 26-27 24.009852008307565 24.27805653797466 27.913877791372972 23.798213662344807 28-29 23.71242996223514 24.137025426926236 28.329791923045732 23.82075268779289 30-31 24.1477378311052 24.010740333040875 28.10176517743066 23.73975665842326 32-33 23.890689957933265 24.15321741767367 27.74903824793633 24.207054376456732 34-35 24.196567664679105 24.12061481609467 27.988074627023558 23.694742892202665 36-37 24.272026855009045 24.036960898740116 27.594163548979715 24.096848697271128 38-39 24.292464306745135 23.85663459063389 27.70403777190873 24.146863330712243 40-41 24.208521246698663 23.992686805572696 27.761795787684633 24.036996160044005 42-43 24.48528722095086 24.015162360673774 27.37840668272231 24.121143735653057 44-45 24.229642767730265 24.29876902788111 26.964981999104364 24.50660620528426 46-47 24.21007274406993 24.34995433661146 26.797878679957826 24.642094239360784 48-49 24.87950154620818 24.563235859335606 26.075191204420356 24.482071390035863 50-51 24.27979844638695 24.981667648106292 25.808672165079322 24.929861740427437 52-53 24.363692140607977 24.904410131277835 25.67678078399983 25.05511694411436 54-55 24.438022969213353 24.85570016608074 25.757056668441486 24.949220196264417 56-57 24.409129856803848 24.6574399588148 25.61013691964302 25.323293264738346 58-59 24.12931195529656 24.67440238609029 26.069939108660467 25.126346549952682 60-61 24.328416732193922 24.73067063473873 25.938349136274365 25.002563496792984 62-63 24.04083258990751 24.750684950828113 25.756386703667527 25.452095755596847 64-65 23.914836898838846 24.545111549134862 25.91871564226702 25.621335909759267 66-67 24.368170326202325 24.563334590986504 25.815823157508717 25.252671925302455 68-69 24.19828136404828 24.332838499702042 25.87298878337923 25.59589135287045 70-71 23.903052571077975 24.404390737560693 25.895414972654855 25.797141718706474 72-73 24.33148446563257 24.571324802448544 25.721767155505876 25.37542357641301 74-75 24.391292625736405 24.54207003561323 25.625340139520585 25.44129719912978 76-77 24.173390831919175 24.708615979588465 25.578873423490993 25.539119765001367 78-79 24.183250830069134 24.84007756644414 25.527659227256606 25.44901237623012 80-81 23.921227629780155 24.80343050294273 25.66703655787493 25.608305309402184 82-83 24.024979107677442 24.795572590683257 25.45364020070734 25.725808100931957 84-85 24.032144920694485 24.864872755810154 25.18547004562764 25.917512277867722 86-87 23.84896376325546 24.71205537654015 25.401116791914763 26.03786406828963 88-89 23.845974718123706 24.521668215113298 25.4796435098318 26.15271355693119 90-91 23.98510179835988 24.676216658493008 25.265721456126922 26.072960087020192 92-93 23.869837634851766 24.705500363760898 25.218101385338027 26.20656061604932 94-95 23.410810092997078 24.71389190160887 25.608579819041218 26.266718186352833 96-97 23.82875836528125 24.700871090128214 25.258785709515273 26.211584835075264 98-99 23.559981867101765 24.798952760245736 25.259108904020323 26.381956468632172 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 105.0 1 134.0 2 469.0 3 809.0 4 593.0 5 496.5 6 994.5 7 2757.0 8 5203.0 9 5176.5 10 3042.5 11 1881.5 12 1925.5 13 2186.0 14 2600.5 15 3148.5 16 3623.0 17 4244.5 18 5109.0 19 6161.0 20 7312.5 21 8939.0 22 11489.0 23 14232.5 24 17007.5 25 20679.5 26 25897.5 27 31212.0 28 36942.0 29 44597.0 30 50779.5 31 57749.5 32 66778.5 33 75216.5 34 82845.0 35 90532.0 36 98117.0 37 108422.5 38 123281.5 39 130516.0 40 136279.0 41 147518.5 42 161118.0 43 182237.5 44 204852.5 45 229493.5 46 260157.0 47 285215.5 48 299969.5 49 311358.0 50 324210.5 51 322132.5 52 313888.0 53 313367.5 54 303759.5 55 293409.5 56 272694.0 57 250593.5 58 236220.5 59 209665.0 60 176644.5 61 141772.5 62 116451.5 63 93353.5 64 70792.5 65 55704.5 66 42350.5 67 32998.5 68 26481.5 69 22698.5 70 19719.0 71 16835.0 72 15938.5 73 13367.0 74 9369.0 75 7361.0 76 5559.5 77 3783.5 78 2406.5 79 1686.0 80 1213.0 81 813.0 82 520.0 83 332.5 84 210.0 85 128.0 86 66.0 87 30.5 88 21.0 89 11.5 90 6.0 91 4.0 92 1.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05710920778428545 2 0.0 3 0.0 4 0.0 5 0.005881585489268222 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014527657203708079 16-17 0.004710910200037377 18-19 6.205989485079179E-4 20-21 2.1156782335497203E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.0451556257647296E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.052260778499068E-6 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.936582544949347E-5 76-77 1.6220199790547855E-4 78-79 4.936582544949347E-5 80-81 3.5261303892495336E-5 82-83 0.0 84-85 4.725014721594375E-4 86-87 8.956371188693816E-4 88-89 3.3850851736795523E-4 90-91 0.0010719436383318582 92-93 0.0014527657203708079 94-95 0.0011072049422243535 96-97 0.0018406400631882564 98-99 6.911215562929086E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7089925.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.51914458988138 #Duplication Level Percentage of deduplicated Percentage of total 1 71.98701993889534 29.16852469498774 2 14.55317948945388 11.793647679513555 3 5.298302275691671 6.44048027968945 4 2.424465292862267 3.929490390185412 5 1.3570061078344393 2.74923633463479 6 0.864559940234118 2.1018737534979257 7 0.5770297536398874 1.636652641427876 8 0.43282804704021527 1.403025777646277 9 0.3258168637531854 1.1881638551013298 >10 1.8712666823245363 14.094450991060514 >50 0.1590253437236572 4.497392311521779 >100 0.12642455054266397 10.55444841040371 >500 0.015186970565305507 4.256916248655374 >1k 0.007714982570301177 5.242391448923746 >5k 6.950434743245351E-5 0.2593490798014576 >10k+ 1.0425652114868027E-4 0.6839561029490933 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22836 0.3220908542756094 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13915 0.19626441746562903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11821 0.16672954932527495 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9454 0.13334414679986037 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 8964 0.12643293123693128 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.4104521556998135E-5 0.0 2 0.0 0.0 0.0 1.4104521556998135E-5 0.0 3 0.0 0.0 0.0 1.4104521556998135E-5 0.0 4 0.0 0.0 0.0 1.4104521556998135E-5 0.0 5 0.0 0.0 0.0 2.820904311399627E-5 0.0 6 0.0 0.0 0.0 2.820904311399627E-5 0.0 7 0.0 0.0 0.0 2.820904311399627E-5 0.0 8 0.0 0.0 0.0 2.820904311399627E-5 1.4104521556998135E-5 9 0.0 0.0 0.0 4.2313564670994404E-5 1.4104521556998135E-5 10-11 0.0 0.0 0.0 4.936582544949347E-5 1.4104521556998135E-5 12-13 0.0 0.0 0.0 5.641808622799254E-5 1.4104521556998135E-5 14-15 0.0 0.0 0.0 7.052260778499067E-5 3.5261303892495336E-5 16-17 0.0 0.0 0.0 8.462712934198881E-5 4.2313564670994404E-5 18-19 0.0 0.0 0.0 9.167939012048788E-5 4.2313564670994404E-5 20-21 1.4104521556998135E-5 0.0 0.0 2.538813880259664E-4 4.2313564670994404E-5 22-23 1.4104521556998135E-5 0.0 0.0 0.001043734595217862 4.936582544949347E-5 24-25 1.4104521556998135E-5 0.0 0.0 0.0031029947425395897 5.641808622799254E-5 26-27 4.2313564670994404E-5 0.0 0.0 0.005035314195848334 5.641808622799254E-5 28-29 4.2313564670994404E-5 0.0 0.0 0.015211726499222488 5.641808622799254E-5 30-31 4.2313564670994404E-5 0.0 0.0 0.04650260757342285 5.641808622799254E-5 32-33 5.641808622799254E-5 0.0 0.0 0.10002221462145228 7.052260778499067E-5 34-35 5.641808622799254E-5 0.0 0.0 0.16964918528757356 9.167939012048788E-5 36-37 6.34703470064916E-5 7.052260778499068E-6 0.0 0.2788393389210746 9.873165089898694E-5 38-39 7.052260778499067E-5 2.1156782335497202E-5 0.0 0.4423177960274615 1.1283617245598508E-4 40-41 7.757486856348974E-5 2.820904311399627E-5 0.0 0.634759888151144 1.1283617245598508E-4 42-43 9.873165089898694E-5 4.2313564670994404E-5 0.0 0.8144091792226293 1.1283617245598508E-4 44-45 9.873165089898694E-5 4.2313564670994404E-5 0.0 1.0238331153009375 1.1283617245598508E-4 46-47 9.873165089898694E-5 5.641808622799254E-5 0.0 1.2521994238302945 1.1283617245598508E-4 48-49 1.0578391167748601E-4 7.757486856348974E-5 0.0 1.493887452970236 1.4104521556998134E-4 50-51 1.1283617245598508E-4 8.462712934198881E-5 0.0 1.7640736679160922 1.4104521556998134E-4 52-53 1.1283617245598508E-4 9.873165089898694E-5 0.0 2.0209029009474713 2.0451556257647293E-4 54-55 1.1283617245598508E-4 9.873165089898694E-5 0.0 2.2782681057980163 2.750381703614636E-4 56-57 1.1283617245598508E-4 9.873165089898694E-5 0.0 2.57139532505633 2.820904311399627E-4 58-59 1.1283617245598508E-4 9.873165089898694E-5 0.0 2.8877809003621335 2.820904311399627E-4 60-61 1.1283617245598508E-4 9.873165089898694E-5 0.0 3.20974481394373 2.961949526969608E-4 62-63 1.1283617245598508E-4 9.873165089898694E-5 0.0 3.531595891352871 2.961949526969608E-4 64-65 1.1283617245598508E-4 9.873165089898694E-5 0.0 3.870619223757656 2.961949526969608E-4 66-67 1.269406940129832E-4 9.873165089898694E-5 0.0 4.235644241652768 2.961949526969608E-4 68-69 1.269406940129832E-4 9.873165089898694E-5 0.0 4.621367644932774 2.961949526969608E-4 70-71 1.269406940129832E-4 9.873165089898694E-5 0.0 5.046449715617584 2.961949526969608E-4 72-73 1.269406940129832E-4 9.873165089898694E-5 0.0 5.468287746344284 3.1029947425395896E-4 74-75 1.3399295479148227E-4 9.873165089898694E-5 0.0 5.890689957933264 3.1029947425395896E-4 76-77 1.4104521556998134E-4 9.873165089898694E-5 0.0 6.3406806136877325 3.1029947425395896E-4 78-79 1.4104521556998134E-4 9.873165089898694E-5 0.0 6.80829486912767 3.1029947425395896E-4 80-81 1.4104521556998134E-4 9.873165089898694E-5 0.0 7.313011068523291 3.244039958109571E-4 82-83 1.4104521556998134E-4 9.873165089898694E-5 0.0 7.845611907037098 3.737698212604506E-4 84-85 1.4104521556998134E-4 9.873165089898694E-5 0.0 8.371442575203545 3.8082208203894964E-4 86-87 1.480974763484804E-4 9.873165089898694E-5 0.0 8.911455339795555 3.878743428174487E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 34875 0.0 37.290546 1 GGTATCA 21010 0.0 28.258934 1 ATCAACG 45705 0.0 28.091204 3 CAACGCA 45670 0.0 28.082188 5 TATCAAC 47055 0.0 27.60151 2 TATCACG 745 0.0 27.464018 2 AACGCAG 47850 0.0 27.189983 6 TCAACGC 47610 0.0 26.996506 4 ACGCAGA 52020 0.0 24.706478 7 CGCAGAG 53000 0.0 24.232096 8 AGTACTT 31645 0.0 23.234385 12-13 GCAGAGT 56140 0.0 22.835665 9 AGAGTAC 53455 0.0 20.823006 10-11 TACTTTT 36030 0.0 20.50347 14-15 GTACTAG 2030 0.0 20.170551 1 GAGTACT 30530 0.0 19.718763 12-13 GTACTTT 33590 0.0 17.936258 14-15 CAGAGTA 54715 0.0 17.42195 9 TAGTACT 3075 0.0 17.088385 4 CATGGGG 14575 0.0 16.845854 4 >>END_MODULE