FastQCFastQC Report
Fri 27 May 2016
ERR765419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10275234
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA634170.6171830247369549No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT532030.517778962503433No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG314160.3057448618688392No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG249430.24274872961530608No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG230130.22396570238692373No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT213930.20819963808123496No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA201820.19641401840580952No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC194710.189494467960535No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC185250.18028786497708957No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC162570.15821537494912524No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT161430.15710591116465086No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC159860.15557796542638347No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157380.15316439508822863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC72800.019.6253133
GATCTAC56300.019.367381
GTATAAG39850.019.3422411
CTACACT89750.019.1143844
CATGGGG211250.018.3774474
TACACTC64350.018.3362085
GTACTAG29050.017.958421
TAGTACT39100.017.7904474
TATTCGC7400.017.1531499
CGTTAAC11450.016.829441
CTAGTAC39950.016.4696983
CTAACGC16300.016.4346943
TCTAACG16650.016.0896132
GTATAAA70800.016.064731
GTCCTAC34550.015.6437571
ATCTACA81300.014.6834512
GTGATCG29900.014.6219248
CTGTGCG51900.014.312049
TACACTA37200.014.0269855
CTAGGCA56750.013.9966054
Produced by FastQC (version 0.11.5)