##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765419_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10275234
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08247997077244	33.0	33.0	33.0	33.0	33.0
2	32.06146254187496	33.0	33.0	33.0	33.0	33.0
3	32.097622399645594	33.0	33.0	33.0	33.0	33.0
4	35.89895879743469	37.0	37.0	37.0	33.0	37.0
5	35.883870576572754	37.0	37.0	37.0	33.0	37.0
6	35.843231307433	37.0	37.0	37.0	33.0	37.0
7	35.797604317332336	37.0	37.0	37.0	33.0	37.0
8	35.76683947051717	37.0	37.0	37.0	33.0	37.0
9	35.76754612109077	37.0	37.0	37.0	33.0	37.0
10-11	35.74518487851469	37.0	37.0	37.0	33.0	37.0
12-13	35.719409309802586	37.0	37.0	37.0	33.0	37.0
14-15	37.46893311626772	40.0	37.0	40.0	33.0	40.0
16-17	37.490169226316404	40.0	37.0	40.0	33.0	40.0
18-19	37.52683569055459	40.0	37.0	40.0	33.0	40.0
20-21	37.53592803823251	40.0	37.0	40.0	33.0	40.0
22-23	37.49493885005441	40.0	37.0	40.0	33.0	40.0
24-25	37.44859761831215	40.0	37.0	40.0	33.0	40.0
26-27	37.38495726715323	40.0	37.0	40.0	33.0	40.0
28-29	37.32963006000642	40.0	37.0	40.0	33.0	40.0
30-31	37.274067724394406	40.0	37.0	40.0	33.0	40.0
32-33	37.181037872227535	40.0	37.0	40.0	33.0	40.0
34-35	37.104007607028706	40.0	37.0	40.0	33.0	40.0
36-37	37.010530709081664	40.0	37.0	40.0	33.0	40.0
38-39	36.91749083281218	40.0	37.0	40.0	33.0	40.0
40-41	36.80877462255361	40.0	37.0	40.0	33.0	40.0
42-43	36.68577216830293	38.5	37.0	40.0	33.0	40.0
44-45	36.48922691200998	37.0	37.0	40.0	33.0	40.0
46-47	36.27977090351422	37.0	37.0	40.0	33.0	40.0
48-49	36.05246045978126	37.0	37.0	40.0	33.0	40.0
50-51	35.84656383494527	37.0	37.0	40.0	33.0	40.0
52-53	35.66242724983198	37.0	37.0	40.0	33.0	40.0
54-55	35.494031620107144	37.0	37.0	40.0	33.0	40.0
56-57	35.30995586085923	37.0	37.0	40.0	27.0	40.0
58-59	35.07682034297224	37.0	33.0	40.0	27.0	40.0
60-61	34.84032417169283	37.0	33.0	40.0	27.0	40.0
62-63	34.55953338872867	37.0	33.0	37.0	27.0	40.0
64-65	34.30314273134802	37.0	33.0	37.0	27.0	40.0
66-67	34.08133688244959	37.0	33.0	37.0	27.0	40.0
68-69	33.80947431464821	37.0	33.0	37.0	27.0	40.0
70-71	33.52134321223244	37.0	33.0	37.0	27.0	38.5
72-73	33.24575678763131	37.0	33.0	37.0	27.0	37.0
74-75	33.01194118790872	37.0	33.0	37.0	27.0	37.0
76-77	32.74508784909424	37.0	33.0	37.0	27.0	37.0
78-79	32.53498713508617	37.0	33.0	37.0	27.0	37.0
80-81	32.35652234294615	37.0	33.0	37.0	27.0	37.0
82-83	32.1871597766046	33.0	33.0	37.0	27.0	37.0
84-85	32.015266367656444	33.0	33.0	37.0	22.0	37.0
86-87	31.836282025304726	33.0	33.0	37.0	22.0	37.0
88-89	31.653516503857723	33.0	33.0	37.0	22.0	37.0
90-91	31.483917689855044	33.0	33.0	37.0	22.0	37.0
92-93	31.303862228344386	33.0	33.0	37.0	22.0	37.0
94-95	31.085363895362384	33.0	33.0	37.0	22.0	37.0
96-97	30.88250442763639	33.0	33.0	37.0	15.0	37.0
98-99	30.59715379717873	33.0	33.0	37.0	15.0	37.0
100	30.334641722027936	33.0	33.0	37.0	2.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56959.0
3	14591.0
4	8834.0
5	7526.0
6	8904.0
7	12845.0
8	14116.0
9	12970.0
10	13295.0
11	15188.0
12	18268.0
13	21790.0
14	23979.0
15	27935.0
16	32897.0
17	38875.0
18	37073.0
19	28632.0
20	24544.0
21	24803.0
22	29206.0
23	37115.0
24	47104.0
25	57263.0
26	67352.0
27	80148.0
28	100282.0
29	130106.0
30	172151.0
31	233306.0
32	325287.0
33	468560.0
34	691075.0
35	1099889.0
36	1989823.0
37	3162409.0
38	1139347.0
39	787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90677133117894	18.257392905275292	12.733764894550859	25.102070868994915
2	15.604356755943352	21.325312146217495	36.8685522218624	26.201778875976743
3	18.22719504340939	25.845385495786076	29.451753666650475	26.47566579415405
4	13.276354657410067	17.57949258258068	35.81127692253631	33.33287583747294
5	14.06574346470833	37.37592618277647	33.599704108175715	14.958626244339488
6	32.07253467460712	36.064569563816754	17.425022944463205	14.43787281711292
7	27.233710654999634	32.085029541962356	21.926434927292554	18.75482487574546
8	27.12157065678664	32.89601550650652	20.372235589172973	19.610178247533863
9	27.303552063139136	14.792745866879342	19.109082772202754	38.794619297778766
10-11	24.812454875798487	25.827456490353672	27.407077908188633	21.95301072565921
12-13	26.372580752618674	23.503430773600964	26.774186122917047	23.349802350863317
14-15	24.35482509915267	23.91394378229111	24.722149143489677	27.00908197506654
16-17	23.669929712999902	26.761249572407408	25.794190736396573	23.774629978196124
18-19	24.295885192332822	26.205000412446793	27.024978702415623	22.47413569280476
20-21	24.881164984628505	25.70914373871178	26.293422994452598	23.116268282207113
22-23	24.459239467244867	25.698243142950254	25.428919484323835	24.41359790548104
24-25	24.21201956776362	26.04321117943231	25.365588863511302	24.379180389292774
26-27	24.485413960390705	25.683930717972668	25.28601172707822	24.54464359455841
28-29	24.263347416532206	25.33605465984992	26.358221221032906	24.04237670258497
30-31	24.922606961709263	25.321209453994292	26.033199129277833	23.722984455018608
32-33	23.82993075393838	25.787362396676155	26.015681409139006	24.36702544024645
34-35	23.77729663718802	25.89037150998027	26.25182710743747	24.080504745394236
36-37	24.544376037698758	25.619509807165645	25.689153627103867	24.146960528031734
38-39	24.768457827821734	25.17030755698605	25.73848926457539	24.322745350616835
40-41	24.216761246073464	25.45812325960461	25.85557963998706	24.469535854334868
42-43	24.96486211408908	25.29224151975517	25.598502184962406	24.144394181193345
44-45	24.87831420676162	25.25700144639042	25.742970914336354	24.12171343251161
46-47	24.379106877386597	25.329986900035262	26.311255570866937	23.9796506517112
48-49	24.803737920260666	25.48522493426867	26.049052757869035	23.661984387601628
50-51	24.04479037223175	25.831114209346378	25.624076000377265	24.500019418044612
52-53	23.945557631003247	26.046093043943923	24.922343489821245	25.086005835231585
54-55	24.21623775236883	25.70270596758975	24.89878185853961	25.182274421501806
56-57	24.629202476588368	25.10707320328619	25.059924291094248	25.203800029031193
58-59	24.31147191765391	25.061029953113493	26.030932552482255	24.596565576750336
60-61	24.563203250797805	25.28614847828161	25.543422177310227	24.60722609361036
62-63	23.660523945748125	25.512397970129776	25.74489641361818	25.08218167050392
64-65	23.527311210461317	25.493602333606113	25.753730653908292	25.22535580202428
66-67	24.50839583916072	25.410276701234224	25.276595860663736	24.804731598941316
68-69	24.471140880867218	24.85964067565207	25.526540015201448	25.142678428279265
70-71	23.85916081660242	25.30966590997648	25.359177997145792	25.471995276275305
72-73	24.619910561350398	25.161520520315463	25.32218321572736	24.89638570260678
74-75	24.827849826002833	24.93508262030558	25.45062546217466	24.786442091516932
76-77	24.228856774599198	25.31246950809269	25.771724776790112	24.686948940518
78-79	24.375455873179614	25.28391953183482	25.58483294022852	24.755791654757044
80-81	24.02996759416994	25.329456514582137	25.599213916875435	25.04136197437249
82-83	24.03860880854699	25.4543030836441	25.157510667848154	25.349577439960765
84-85	24.22667910543109	25.44152722094319	24.911112918486232	25.420680755139497
86-87	24.16035631838982	24.91003026845257	25.34585529914005	25.583758114017563
88-89	24.306168981172764	24.53590951649547	25.71114917189245	25.446772330439316
90-91	24.418599330808043	24.926090990246387	25.45249798239696	25.20281169654861
92-93	23.939266975748804	24.964366254741023	25.590504845624746	25.505861923885426
94-95	23.418062510086653	25.141982101874362	25.92670174599099	25.513253642048
96-97	24.10968210474744	24.959189691918983	25.435582110816846	25.495546092516737
98-99	24.13544054972047	24.81002493287397	25.27421051171524	25.780324005690318
100	23.839170626269166	25.026005042452436	25.586723105744234	25.548101225534165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4228.0
1	3471.5
2	2761.0
3	2615.0
4	1938.0
5	1605.0
6	2167.5
7	4116.5
8	6736.0
9	6370.5
10	3533.5
11	2058.5
12	1991.5
13	2112.0
14	2304.0
15	2677.0
16	3159.5
17	3643.0
18	4364.5
19	5346.0
20	6704.0
21	8541.5
22	10713.5
23	13175.0
24	16133.5
25	20487.0
26	26979.0
27	33727.0
28	39719.5
29	48538.0
30	58039.5
31	67289.0
32	77908.5
33	90575.0
34	104496.0
35	115553.5
36	128464.5
37	146032.5
38	162479.0
39	182495.5
40	201354.0
41	217106.0
42	233790.0
43	257217.5
44	286791.0
45	318855.5
46	354679.5
47	394783.0
48	461576.5
49	522195.0
50	596559.0
51	597427.0
52	509433.5
53	471629.0
54	451754.0
55	416388.5
56	383969.5
57	351186.0
58	323023.5
59	294717.0
60	249810.0
61	205405.5
62	164268.5
63	128613.5
64	97969.0
65	72480.0
66	55955.0
67	44329.0
68	35456.0
69	29572.5
70	24416.5
71	19846.5
72	16310.0
73	14636.0
74	12045.0
75	9186.0
76	7168.5
77	5332.5
78	3997.0
79	2943.0
80	2139.0
81	1554.5
82	1174.0
83	953.5
84	710.5
85	455.0
86	312.5
87	199.5
88	131.5
89	88.5
90	59.0
91	43.0
92	23.0
93	16.5
94	12.5
95	10.0
96	6.5
97	5.5
98	4.5
99	10.5
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007347764537527807
2	0.014043475798215398
3	0.010462048844824361
4	0.01865650942839842
5	0.011766155398504794
6	0.004126426707167934
7	0.0034841055687880196
8	0.023211150227819628
9	0.010082495444872594
10-11	0.01466146659044456
12-13	0.010642093406339944
14-15	0.009021692352699706
16-17	0.01112870032935503
18-19	0.011498521590846496
20-21	0.006763836229909703
22-23	0.005912274114633302
24-25	0.012607985375320895
26-27	0.016160215913331024
28-29	0.0033381234918834937
30-31	8.272317691256472E-4
32-33	0.0013333029690613372
34-35	0.0010997316460140956
36-37	9.148210152683627E-4
38-39	0.0
40-41	0.0026276773842814675
42-43	0.0
44-45	0.0
46-47	1.5084814613467685E-4
48-49	0.006262631099204164
50-51	0.012505797921487724
52-53	0.018963071789897923
54-55	0.02068079422814118
56-57	0.034286323795643
58-59	0.03939569648730141
60-61	0.032057664088233906
62-63	0.022014097197202515
64-65	0.019804901766714025
66-67	0.02198003471259146
68-69	0.018194232851534087
70-71	0.019318294843698937
72-73	0.021006820866561287
74-75	0.0174302599824004
76-77	0.009649415283389166
78-79	0.016354858682537058
80-81	0.009756468806452485
82-83	0.016773340636330035
84-85	0.024422801466127197
86-87	0.014607939828912899
88-89	0.027016416365797605
90-91	0.030597843319188644
92-93	0.02596534541208502
94-95	0.01708963513628984
96-97	0.023361998373954307
98-99	0.016048296321037554
100	0.012311155152281691
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0275234E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.729517503323294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.63841093820042	27.745210903413003
2	14.483229779535217	11.218570025023247
3	5.503156144665649	6.394037466850482
4	2.5503023807159013	3.9508792277081426
5	1.4745849722185755	2.855498224583841
6	0.8708029893031504	2.023546776969756
7	0.5907402453169852	1.6015359269645173
8	0.4271226897094298	1.323380454973431
9	0.3368961009420642	1.1743041094413342
>10	1.8160339920954813	12.900592219135257
>50	0.1548709150646041	4.206632949411296
>100	0.12780684098009643	10.217651368669703
>500	0.0167437893228442	4.419907913334704
>1k	0.00846892129661068	5.371104994633589
>5k	5.026064442657191E-4	1.2910239580521612
>10k+	3.266941887727174E-4	3.306123480835189
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	63417	0.6171830247369549	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	53203	0.517778962503433	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	31416	0.3057448618688392	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	24943	0.24274872961530608	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	23013	0.22396570238692373	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	21393	0.20819963808123496	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	20182	0.19641401840580952	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	19471	0.189494467960535	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	18525	0.18028786497708957	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	16257	0.15821537494912524	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	16143	0.15710591116465086	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	15986	0.15557796542638347	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15738	0.15316439508822863	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.211605691899571E-4	0.0	0.0	1.946427692060346E-5	9.73213846030173E-6
2	4.8660692301508656E-4	0.0	0.0	2.9196415380905195E-5	9.73213846030173E-6
3	5.158033383959918E-4	0.0	0.0	4.866069230150866E-5	9.73213846030173E-6
4	5.741961691578021E-4	0.0	0.0	4.866069230150866E-5	2.9196415380905195E-5
5	0.001917231276679441	0.0	0.0	1.557142153648277E-4	2.9196415380905195E-5
6	0.004768747845547848	0.0	0.0	3.698212614914658E-4	3.892855384120692E-5
7	0.005518122506991081	0.0	0.0	4.963390614753883E-4	3.892855384120692E-5
8	0.006588657737624272	0.0	0.0	5.449997537768969E-4	4.866069230150866E-5
9	0.007415889506749919	0.0	0.0	5.839283076181039E-4	4.866069230150866E-5
10-11	0.013878029444390268	0.0	0.0	0.001089999507553794	4.866069230150866E-5
12-13	0.024753694173777457	1.946427692060346E-5	0.0	0.0018150438228462728	5.8392830761810384E-5
14-15	0.03832029518743807	1.946427692060346E-5	0.0	0.0027152666304241834	7.299103845226297E-5
16-17	0.04428122999437288	1.946427692060346E-5	0.0	0.0031045521688362523	8.758924614271558E-5
18-19	0.04784319267084331	3.4062484611056064E-5	0.0	0.003367319907264399	8.758924614271558E-5
20-21	0.05902541976173	4.866069230150866E-5	0.0	0.004165355261009141	8.758924614271558E-5
22-23	0.06231001649208183	4.866069230150866E-5	0.0	0.005138569107039314	1.0218745383316818E-4
24-25	0.07661626002872537	5.839283076181039E-5	0.0	0.0074645501990514285	1.1678566152362078E-4
26-27	0.11507767122383783	5.839283076181039E-5	0.0	0.012072717760004298	1.4598207690452597E-4
28-29	0.12343757816123702	5.839283076181039E-5	0.0	0.02511864936603877	1.557142153648277E-4
30-31	0.1268146302069617	5.839283076181039E-5	0.0	0.055901403315973146	1.6058028459497856E-4
32-33	0.12999703948348038	6.325889999196126E-5	0.0	0.10434798857135516	1.7517849228543115E-4
34-35	0.13204078856014373	6.812496922211211E-5	0.0	0.16935380741694056	1.849106307457329E-4
36-37	0.1376124378286665	6.812496922211211E-5	0.0	0.2687919321350735	1.849106307457329E-4
38-39	0.14330087275871284	7.785710768241385E-5	0.0	0.42572753087666904	2.0437490766633636E-4
40-41	0.14811828129656218	7.785710768241385E-5	0.0	0.6187888275829047	2.0437490766633636E-4
42-43	0.15938323156436146	7.785710768241385E-5	0.0	0.7886389740613207	2.1897311535678897E-4
44-45	0.1722442525396502	7.785710768241385E-5	0.0	0.9703234009074635	2.3357132304724154E-4
46-47	0.17684755403137292	8.272317691256472E-5	0.0	1.1681485793900168	2.53035599967845E-4
48-49	0.17991317764636794	8.758924614271558E-5	0.0	1.3771511188942265	2.7249987688844846E-4
50-51	0.18527071986876406	1.1678566152362078E-4	0.0	1.6134717710565034	2.822320153487502E-4
52-53	0.18689598699163446	1.265177999839225E-4	0.0	1.8900932085828897	3.3575877688040974E-4
54-55	0.19238004701401448	1.3138386921407338E-4	0.0	2.1271583693373795	3.503569845708623E-4
56-57	0.20326544388186196	1.5084814613467682E-4	0.0	2.402008557663991	3.552230538010132E-4
58-59	0.20673008517372937	1.6544635382512943E-4	0.0	2.7048435101332	3.6008912303116405E-4
60-61	0.20970325347335156	1.6544635382512943E-4	0.0	2.996364851642308	3.6008912303116405E-4
62-63	0.2124720468653074	1.7517849228543115E-4	0.0	3.297910295765527	3.9901767687237097E-4
64-65	0.21445740311120895	1.849106307457329E-4	0.0	3.595348777458499	3.9901767687237097E-4
66-67	0.21741597320314066	1.849106307457329E-4	0.0	3.955184864889695	4.3794623071357795E-4
68-69	0.22032101653354075	1.849106307457329E-4	0.0	4.34465044786328	4.4767836917387964E-4
70-71	0.2240776219792172	1.849106307457329E-4	0.0	4.764636017048371	4.6714264609448307E-4
72-73	0.2347537778701682	1.8977669997588377E-4	0.0	5.170076905304541	5.40133684546746E-4
74-75	0.24583381750722172	1.9464276920603464E-4	0.0	5.57387792823015	5.595979614673495E-4
76-77	0.24906488747604189	1.9464276920603464E-4	0.0	5.977070692501991	5.839283076181039E-4
78-79	0.25164390416802185	1.9464276920603464E-4	0.0	6.3896938989418635	5.936604460784056E-4
80-81	0.25642238415203	1.9464276920603464E-4	0.0	6.884320104048239	5.985265153085565E-4
82-83	0.2579454638210672	1.9950883843618548E-4	0.0	7.409222991904612	6.228568614593108E-4
84-85	0.2635706398511216	2.1897311535678897E-4	0.0	7.9269143651619025	6.228568614593108E-4
86-87	0.2738964387575018	2.2383918458693982E-4	0.0	8.436323688589475	6.277229306894616E-4
88	0.2762856787495058	2.3357132304724156E-4	0.0	8.831497170770028	6.325889999196125E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACAC	7280	0.0	19.625313	3
GATCTAC	5630	0.0	19.36738	1
GTATAAG	3985	0.0	19.342241	1
CTACACT	8975	0.0	19.114384	4
CATGGGG	21125	0.0	18.377447	4
TACACTC	6435	0.0	18.336208	5
GTACTAG	2905	0.0	17.95842	1
TAGTACT	3910	0.0	17.790447	4
TATTCGC	740	0.0	17.153149	9
CGTTAAC	1145	0.0	16.82944	1
CTAGTAC	3995	0.0	16.469698	3
CTAACGC	1630	0.0	16.434694	3
TCTAACG	1665	0.0	16.089613	2
GTATAAA	7080	0.0	16.06473	1
GTCCTAC	3455	0.0	15.643757	1
ATCTACA	8130	0.0	14.683451	2
GTGATCG	2990	0.0	14.621924	8
CTGTGCG	5190	0.0	14.31204	9
TACACTA	3720	0.0	14.026985	5
CTAGGCA	5675	0.0	13.996605	4
>>END_MODULE