FastQCFastQC Report
Fri 27 May 2016
ERR765417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5754329
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA211820.3681054732880237No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT196440.341377769675665No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG81510.141649877857175No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG76030.13212661285095098No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT67220.11681640031357263No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG61700.1072236224240915No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC60080.10440835065217856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA16350.031.2840041
GTACTAG25000.026.5976621
GTACAAA36450.024.4935171
TAGGCAT37350.021.785175
TAGTACT30250.021.5187054
TATTCGC4800.021.3116749
GTATAAG27850.021.0374341
TCTAACG13050.020.6664032
CTAGGCA40550.020.5246454
TACATAG16650.020.1078552
CTAACGC13450.020.051793
GTACAAG36850.019.9373321
CTGTGCG35300.019.2315489
TACATGA23500.018.7976342
GTGATCG21100.018.73028
CTAGTAC37650.018.5256613
ATAAGGT24700.018.2609023
CATGGGG158600.017.9709384
ATACGGG18000.017.8247743
TACTAGG39050.017.7424132
Produced by FastQC (version 0.11.5)