FastQCFastQC Report
Fri 27 May 2016
ERR765417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5754329
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT224340.389863005747499No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA211890.3682271208337236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209200.363552379434683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143290.24901252604778074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105390.1831490691616694No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG85690.14891397415754296No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG75740.13162264444733696No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT72570.126113748449211No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG71750.12468873434244028No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC63540.11042121505391853No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC58880.10232296415446528No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC58510.10167996998433702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4600.042.4583852
GTATCAA569750.027.1457351
TCACGCA7600.025.6980514
AGTACTT347900.023.27228512-13
GTACTAG23550.022.5236571
ATCACGC9200.022.239723
CAACGCA693650.022.1897325
ATCAACG698300.021.9820383
AACGCAG722100.021.6692896
TATCAAC722200.021.4674342
TCAACGC725000.021.2558754
TACTTTT400150.020.48326114-15
GAGTACT339050.020.29298212-13
ACGCAGA759000.020.0705417
CGCAGAG763700.019.9532838
TAGGCAT34950.019.8245625
TAACGCA5050.019.3371474
GTATAAG24550.019.331861
GCAGAGT787300.019.3144939
CTAGGCA37250.018.7253284
Produced by FastQC (version 0.11.5)