##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765417_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5754329 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28085568273903 33.0 33.0 33.0 33.0 33.0 2 32.1342384142443 33.0 33.0 33.0 27.0 33.0 3 32.183971754135015 33.0 33.0 33.0 33.0 33.0 4 36.10010585074298 37.0 37.0 37.0 33.0 37.0 5 36.11109670649697 37.0 37.0 37.0 33.0 37.0 6 36.136019334313346 37.0 37.0 37.0 33.0 37.0 7 36.04838930829294 37.0 37.0 37.0 33.0 37.0 8 36.00569362648538 37.0 37.0 37.0 33.0 37.0 9 35.73869481567703 37.0 37.0 37.0 33.0 37.0 10-11 35.91190606237495 37.0 37.0 37.0 33.0 37.0 12-13 35.84647949396011 37.0 37.0 37.0 33.0 37.0 14-15 37.20364998247406 37.0 37.0 40.0 33.0 40.0 16-17 37.35447373620799 37.0 37.0 40.0 33.0 40.0 18-19 37.44008545566303 37.0 37.0 40.0 33.0 40.0 20-21 37.467890608965874 37.0 37.0 40.0 33.0 40.0 22-23 37.38944532021023 37.0 37.0 40.0 33.0 40.0 24-25 37.33519555451209 37.0 37.0 40.0 33.0 40.0 26-27 37.200320489148254 37.0 37.0 40.0 33.0 40.0 28-29 37.11957380261018 37.0 37.0 40.0 33.0 40.0 30-31 36.97966079103229 37.0 37.0 40.0 33.0 40.0 32-33 36.77025488115122 37.0 37.0 40.0 33.0 40.0 34-35 36.657715174088935 37.0 37.0 40.0 33.0 40.0 36-37 36.48437167913062 37.0 37.0 40.0 33.0 40.0 38-39 36.2988398821131 37.0 37.0 40.0 33.0 40.0 40-41 36.125381430224095 37.0 37.0 40.0 33.0 40.0 42-43 35.8998127322925 37.0 37.0 40.0 33.0 40.0 44-45 35.62563280618818 37.0 37.0 40.0 27.0 40.0 46-47 35.28206225261017 37.0 35.0 40.0 27.0 40.0 48-49 34.9558392472867 37.0 33.0 40.0 27.0 40.0 50-51 34.61502401061878 37.0 33.0 40.0 22.0 40.0 52-53 34.45092946545114 37.0 33.0 40.0 22.0 40.0 54-55 34.32197194494788 37.0 33.0 40.0 24.5 40.0 56-57 34.147403893659884 37.0 33.0 40.0 22.0 40.0 58-59 33.9622419051813 37.0 33.0 37.0 22.0 40.0 60-61 33.77652737617193 37.0 33.0 37.0 22.0 40.0 62-63 33.44965268756792 37.0 33.0 37.0 22.0 40.0 64-65 33.31811076495626 37.0 33.0 37.0 22.0 40.0 66-67 33.14218556151377 37.0 33.0 37.0 22.0 40.0 68-69 32.912219826151755 37.0 33.0 37.0 22.0 40.0 70-71 32.71703234208542 37.0 33.0 37.0 22.0 37.0 72-73 32.522954804982476 37.0 33.0 37.0 22.0 37.0 74-75 32.325649350254395 35.0 33.0 37.0 22.0 37.0 76-77 32.17286802683684 33.0 33.0 37.0 22.0 37.0 78-79 32.00151807447923 33.0 33.0 37.0 22.0 37.0 80-81 31.870031588391974 33.0 33.0 37.0 22.0 37.0 82-83 31.73455210850822 33.0 33.0 37.0 22.0 37.0 84-85 31.58897475274702 33.0 33.0 37.0 22.0 37.0 86-87 31.49465984652598 33.0 33.0 37.0 22.0 37.0 88-89 31.39679847989227 33.0 33.0 37.0 22.0 37.0 90-91 31.344095984084333 33.0 33.0 37.0 18.5 37.0 92-93 31.24235614612929 33.0 33.0 37.0 15.0 37.0 94-95 31.17942856586754 33.0 33.0 37.0 15.0 37.0 96-97 31.137373619061407 33.0 33.0 37.0 15.0 37.0 98-99 30.328344886084892 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18273257671749832 1101 2 0.3513617189040943 1101 3 0.2797971203710503 1101 4 0.27257232399207254 1101 5 0.10452743753933191 1101 6 0.20202135774841423 1101 7 0.20258227711956778 1101 8 0.17663711726009268 1101 9 0.41392963854006126 1101 10-11 0.4099529515112721 1101 12-13 0.3444207126443217 1101 14-15 0.6392809735706066 1101 16-17 0.5582645336969279 1101 18-19 0.33778180902321964 1101 20-21 0.4123345432550565 1101 22-23 0.26513018122978593 1101 24-25 0.432960719865747 1101 26-27 0.30203241752887067 1101 28-29 0.3391785114388881 1101 30-31 0.35906702365078047 1101 32-33 0.4700023531302122 1101 34-35 0.5041132958826822 1101 36-37 0.4679461808315182 1101 38-39 0.26985938861940184 1101 40-41 0.23981900074691964 1101 42-43 0.43919121461264155 1101 44-45 0.44078134749620546 1101 46-47 0.570268501957429 1101 48-49 0.5893739847808419 1101 50-51 0.5718183570057462 1101 52-53 0.5373579518476745 1101 54-55 0.6091747372182752 1101 56-57 0.6910079999641638 1101 58-59 0.5837105413749413 1101 60-61 0.4782626723746404 1101 62-63 0.5008983062396553 1101 64-65 0.5029654128185328 1101 66-67 0.48459639766298324 1101 68-69 0.43520033706536054 1101 70-71 0.44206564302062645 1101 72-73 0.5253320496806069 1101 74-75 0.4023769828856132 1101 76-77 0.36124877805364264 1101 78-79 0.6416057852184274 1101 80-81 0.5882817376901244 1101 82-83 0.5956532801797856 1101 84-85 0.6015205277698001 1101 86-87 0.4231629446288636 1101 88-89 0.5546749895966485 1101 90-91 0.46649572130672823 1101 92-93 0.6326447982763064 1101 94-95 0.6331300357279659 1101 96-97 0.505651384558174 1101 98-99 0.6256107669408131 1102 1 0.12897094256049257 1102 2 0.3254345439789077 1102 3 0.2377088178704696 1102 4 0.14044685646558008 1102 5 0.07756557859203639 1102 6 0.18346840754342963 1102 7 0.20101106425607185 1102 8 0.09119452649923687 1102 9 0.3684900240176532 1102 10-11 0.27937768622160775 1102 12-13 0.32070755252924954 1102 14-15 0.5704700313545388 1102 16-17 0.3464740095708905 1102 18-19 0.2904053967332558 1102 20-21 0.366410827874347 1102 22-23 0.27615997156271277 1102 24-25 0.4027418132888343 1102 26-27 0.28299521242873027 1102 28-29 0.2605073314185091 1102 30-31 0.2846317461653456 1102 32-33 0.36691229007467285 1102 34-35 0.39133169618359176 1102 36-37 0.40114802584304243 1102 38-39 0.25748525089922225 1102 40-41 0.2763800974557924 1102 42-43 0.33031429508548626 1102 44-45 0.34636737318422917 1102 46-47 0.4393927107687077 1102 48-49 0.4396082846917295 1102 50-51 0.4154846241131409 1102 52-53 0.4556714265035353 1102 54-55 0.41629475060789645 1102 56-57 0.49600242801991357 1102 58-59 0.43162706109045246 1102 60-61 0.3418446598818292 1102 62-63 0.4694283554777243 1102 64-65 0.33853405501645994 1102 66-67 0.434476638323261 1102 68-69 0.34090302410393036 1102 70-71 0.36878009044015414 1102 72-73 0.40472638219899437 1102 74-75 0.3544720516705766 1102 76-77 0.36476296864321966 1102 78-79 0.5300686800573935 1102 80-81 0.42289482767776576 1102 82-83 0.4258762336128932 1102 84-85 0.4487547011979416 1102 86-87 0.38155864440300036 1102 88-89 0.4264156868675073 1102 90-91 0.3945059328902687 1102 92-93 0.413388520732358 1102 94-95 0.47036615766068124 1102 96-97 0.3533300467276561 1102 98-99 0.2893596367416187 1103 1 0.10560138965980315 1103 2 0.23616659837713172 1103 3 0.20235069239395642 1103 4 0.18142474938903774 1103 5 0.12057054467978645 1103 6 0.15888885651425966 1103 7 0.18722321337762793 1103 8 0.10283626342405938 1103 9 0.38648962798519904 1103 10-11 0.2986203668107663 1103 12-13 0.27451699789356354 1103 14-15 0.5234489687300297 1103 16-17 0.45198210653217785 1103 18-19 0.24439369209618889 1103 20-21 0.290318968450336 1103 22-23 0.2415717644516988 1103 24-25 0.2943229502667606 1103 26-27 0.157144960579906 1103 28-29 0.0834603042354729 1103 30-31 0.12390815956641177 1103 32-33 0.1865717437003127 1103 34-35 0.3301127396396524 1103 36-37 0.24067573870101455 1103 38-39 0.17746836372320018 1103 40-41 0.09341747061024819 1103 42-43 0.13409582620812444 1103 44-45 0.1752732580564711 1103 46-47 0.23777687400290404 1103 48-49 0.23843331424790648 1103 50-51 0.23694044946007153 1103 52-53 0.21844699437824033 1103 54-55 0.19648356729230443 1103 56-57 0.2799360582694348 1103 58-59 0.31191557044168405 1103 60-61 0.24255304598221272 1103 62-63 0.2340468679697807 1103 64-65 0.04314784299269547 1103 66-67 0.11620753834782249 1103 68-69 0.06544219516020888 1103 70-71 0.0658957589069189 1103 72-73 0.18959327085227784 1103 74-75 0.1175300065178746 1103 76-77 0.17200196120467837 1103 78-79 0.3284780561946796 1103 80-81 0.171084844798699 1103 82-83 0.28289375573868014 1103 84-85 0.3036213715811016 1103 86-87 0.2155077919868873 1103 88-89 0.25091323420447154 1103 90-91 0.07348237087621357 1103 92-93 0.014575963052390506 1103 94-95 0.2597171270233716 1103 96-97 0.2102819034665515 1103 98-99 0.10052116218825446 1104 1 0.11836224004093765 1104 2 0.30788839098057963 1104 3 0.2204837087986533 1104 4 0.24284754971323252 1104 5 0.07529005708658332 1104 6 0.14405385964324324 1104 7 0.153749601541314 1104 8 0.09220635178542835 1104 9 0.42994090104377136 1104 10-11 0.27594253809806446 1104 12-13 0.25451152125192067 1104 14-15 0.43536556171019214 1104 16-17 0.32936510699325794 1104 18-19 0.24021644367246608 1104 20-21 0.31955245472610727 1104 22-23 0.2738221055862837 1104 24-25 0.3288440964317658 1104 26-27 0.16095441770448815 1104 28-29 0.16893823144707198 1104 30-31 0.22253898194625066 1104 32-33 0.27721857721753196 1104 34-35 0.3306806744584705 1104 36-37 0.23640194344785925 1104 38-39 0.06051763294458823 1104 40-41 0.05175041467489194 1104 42-43 0.18279400887080044 1104 44-45 0.15588607326024118 1104 46-47 0.15136078057330593 1104 48-49 0.23052631453639805 1104 50-51 0.12933956121940327 1104 52-53 0.16613527556981467 1104 54-55 0.09914681539316916 1104 56-57 0.15352381810813398 1104 58-59 0.18714059437678543 1104 60-61 0.1430147706837559 1104 62-63 0.11995531979281537 1104 64-65 0.09653501898736039 1104 66-67 0.05360352677911351 1104 68-69 0.0658920586399887 1104 70-71 0.15714942747745653 1104 72-73 0.18114869927951105 1104 74-75 0.13988432836649878 1104 76-77 0.1405479285835014 1104 78-79 0.2352100819753069 1104 80-81 0.20540853339797138 1104 82-83 0.15624923280564573 1104 84-85 0.21759210468942314 1104 86-87 0.16083682835119006 1104 88-89 0.19564542333043988 1104 90-91 0.15169673493009128 1104 92-93 0.13791658439700427 1104 94-95 0.1423391943488923 1104 96-97 0.13770745263411044 1104 98-99 0.13387393682907245 1105 1 0.10466005326212979 1105 2 0.27985462798644534 1105 3 0.22465199184639317 1105 4 0.1837931522574081 1105 5 -0.0033240305618349453 1105 6 0.08476532848970209 1105 7 0.08451471953799938 1105 8 0.042915258958117874 1105 9 0.3201006367028896 1105 10-11 0.21056434081926056 1105 12-13 0.23599725675615701 1105 14-15 0.42932447674553487 1105 16-17 0.3087185943096813 1105 18-19 0.1506782621712759 1105 20-21 0.23742795543417117 1105 22-23 0.13414178495305862 1105 24-25 0.2946269542170228 1105 26-27 0.14310687580110226 1105 28-29 0.16320848322413894 1105 30-31 0.19919432902810286 1105 32-33 0.2248818665830683 1105 34-35 0.2531247874176401 1105 36-37 0.1889576829688906 1105 38-39 0.13742890407193187 1105 40-41 0.029889786639600402 1105 42-43 0.21777895363847222 1105 44-45 0.2569919851178568 1105 46-47 0.3463788087098578 1105 48-49 0.3017161228829295 1105 50-51 0.18719399305798845 1105 52-53 0.13328327856838484 1105 54-55 0.16740775036699063 1105 56-57 0.2396309870777955 1105 58-59 0.205315137495802 1105 60-61 0.11552571771769493 1105 62-63 0.20347755248707955 1105 64-65 0.10881419629041034 1105 66-67 0.09843107795464334 1105 68-69 0.1113333773611771 1105 70-71 0.18806189494478076 1105 72-73 0.22570181495553499 1105 74-75 0.11858305284032866 1105 76-77 0.12434206004500226 1105 78-79 0.22565987502479956 1105 80-81 0.25230503612016264 1105 82-83 0.1664811941602018 1105 84-85 0.16514714189452917 1105 86-87 0.15491006058793744 1105 88-89 0.18622948069197776 1105 90-91 0.06415822237621427 1105 92-93 0.057800203743351375 1105 94-95 0.2686627982899168 1105 96-97 0.16644641503517477 1105 98-99 0.0651370573467922 1106 1 0.08851941744565295 1106 2 0.23096753481929255 1106 3 0.25562118191848526 1106 4 0.2535399021304343 1106 5 0.012815586520112277 1106 6 0.13603195508614618 1106 7 0.15049608106213697 1106 8 0.04487175074687855 1106 9 0.3883488765964316 1106 10-11 0.2438925926077431 1106 12-13 0.2232957353003897 1106 14-15 0.4010158816823193 1106 16-17 0.31117900798381726 1106 18-19 0.17415690343167967 1106 20-21 0.2677878876834683 1106 22-23 0.1769312244113621 1106 24-25 0.2222408329711243 1106 26-27 0.1030911290759775 1106 28-29 0.1874499322818366 1106 30-31 0.10220911620233153 1106 32-33 0.15018670469888207 1106 34-35 0.23944713030331144 1106 36-37 0.1323388616544463 1106 38-39 0.13982065018776524 1106 40-41 0.0255483039151585 1106 42-43 0.10572622054351655 1106 44-45 0.12184761466911453 1106 46-47 0.23693057352190294 1106 48-49 0.354496588987935 1106 50-51 0.21497817633123617 1106 52-53 0.2504194241911506 1106 54-55 0.23658217760775813 1106 56-57 0.2674594428557455 1106 58-59 0.2813954993988119 1106 60-61 0.26985402359613886 1106 62-63 0.20225841226021402 1106 64-65 0.08864630277630425 1106 66-67 0.1763228104873562 1106 68-69 0.07343157754833385 1106 70-71 0.12037919824776111 1106 72-73 0.2354667613676753 1106 74-75 0.14333561556404817 1106 76-77 0.18324690344852712 1106 78-79 0.21886257505938644 1106 80-81 0.1853234895259135 1106 82-83 0.10035492977724658 1106 84-85 0.09676943782138281 1106 86-87 0.08630177686939788 1106 88-89 0.017699627404304863 1106 90-91 0.08166935326371316 1106 92-93 -0.012842280591176092 1106 94-95 0.21269536068376738 1106 96-97 0.21730056329791836 1106 98-99 0.06372741982708874 1107 1 0.09571915154264587 1107 2 0.225956557420659 1107 3 0.18387805846356997 1107 4 0.17637168210575283 1107 5 -0.013748789519056004 1107 6 0.12185100948211414 1107 7 0.12836218040198588 1107 8 0.019262770174584887 1107 9 0.3304330868254226 1107 10-11 0.24148004130475442 1107 12-13 0.1483629945995446 1107 14-15 0.29596063870725686 1107 16-17 0.2431088342762564 1107 18-19 0.21544688706811854 1107 20-21 0.31743326489080204 1107 22-23 0.16974999073284636 1107 24-25 0.24668174790653552 1107 26-27 0.047733959271440085 1107 28-29 0.09046287899560213 1107 30-31 0.07727292209004588 1107 32-33 0.057756800245201134 1107 34-35 0.2360976839519111 1107 36-37 0.18545361145526584 1107 38-39 0.07011305643586496 1107 40-41 0.15935406780396022 1107 42-43 0.16852525018327214 1107 44-45 0.08779724380948295 1107 46-47 0.10632335061028186 1107 48-49 0.24221844407156112 1107 50-51 0.07207709886686331 1107 52-53 0.030306185185288825 1107 54-55 0.17830242535568885 1107 56-57 0.2079102475102843 1107 58-59 0.13944449701953943 1107 60-61 0.13546454127509122 1107 62-63 0.21799612575746607 1107 64-65 -0.01418146563114675 1107 66-67 -0.04445211472742727 1107 68-69 0.0024770314078566003 1107 70-71 0.05975402823737852 1107 72-73 0.19273306795090406 1107 74-75 0.10559052975886374 1107 76-77 0.11520449246044961 1107 78-79 0.14784292679273037 1107 80-81 0.06979921039894776 1107 82-83 0.012616465429029944 1107 84-85 0.07754259376738304 1107 86-87 0.1359882661177778 1107 88-89 -0.011933327195229992 1107 90-91 -0.018292518945749237 1107 92-93 0.014202042251909575 1107 94-95 0.18476183275988944 1107 96-97 0.0948143953232119 1107 98-99 0.08088753739398413 1108 1 0.11491389056633494 1108 2 0.24938339676407395 1108 3 0.1444238927553272 1108 4 0.05270311998902599 1108 5 -0.013138265783325664 1108 6 0.10111011795312663 1108 7 0.04344621301716245 1108 8 0.052939887864489776 1108 9 0.2911263412622276 1108 10-11 0.22054261568650446 1108 12-13 0.1575990486128589 1108 14-15 0.2532136821332571 1108 16-17 0.2423873177253668 1108 18-19 0.07076437603702601 1108 20-21 0.22077013119923805 1108 22-23 0.10285763004090853 1108 24-25 0.105821883645838 1108 26-27 0.026813437090879688 1108 28-29 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5 -0.338509511545638 2216 6 -0.36182499163003 2216 7 -0.3487253956348937 2216 8 -0.2609983647034042 2216 9 -0.05314845503360033 2216 10-11 -0.3231575817682142 2216 12-13 -0.24599993574523893 2216 14-15 -0.6977456444422359 2216 16-17 -0.7934332990186803 2216 18-19 -0.7658341224083287 2216 20-21 -1.070309859026338 2216 22-23 -0.9712784704671975 2216 24-25 -0.785578724728154 2216 26-27 -0.8776913672660456 2216 28-29 -1.0308497429769403 2216 30-31 -0.6117382075346853 2216 32-33 -0.7724775761545288 2216 34-35 -0.741027024381232 2216 36-37 -0.7784200650811286 2216 38-39 -0.7671314717069322 2216 40-41 -0.7182084200720524 2216 42-43 -0.7987356216996346 2216 44-45 -0.5996207084696294 2216 46-47 -0.9146591893029239 2216 48-49 -1.1133735304420256 2216 50-51 -0.7159079407162139 2216 52-53 -0.6068363798285787 2216 54-55 -0.5807455788787763 2216 56-57 -0.7239389939291598 2216 58-59 -0.7533928848676865 2216 60-61 -0.6601000211433288 2216 62-63 -0.8853009180140745 2216 64-65 -0.9359100135598482 2216 66-67 -0.7695547303672967 2216 68-69 -0.6546779058770014 2216 70-71 -0.7288104742385144 2216 72-73 -0.5784976749582427 2216 74-75 -0.6714986277875745 2216 76-77 -0.614623408332232 2216 78-79 -0.5469158288534253 2216 80-81 -0.4984777371416058 2216 82-83 -0.4654021828912427 2216 84-85 -0.5262173456884476 2216 86-87 -0.7075488176272486 2216 88-89 -0.5494829539700348 2216 90-91 -0.5092309115171219 2216 92-93 -0.5710008731669696 2216 94-95 -0.596871786992569 2216 96-97 -0.5778990570413889 2216 98-99 -0.4913706392869557 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 10.0 9 31.0 10 78.0 11 191.0 12 320.0 13 582.0 14 1450.0 15 3776.0 16 8738.0 17 16652.0 18 27027.0 19 38373.0 20 49429.0 21 62448.0 22 73318.0 23 67927.0 24 52146.0 25 44371.0 26 49518.0 27 63099.0 28 84190.0 29 111774.0 30 149305.0 31 198840.0 32 263851.0 33 350198.0 34 465664.0 35 654257.0 36 1040982.0 37 1406750.0 38 468343.0 39 690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63667483499178 19.816218770849357 13.549357798547993 23.997748595610872 2 15.81567373537211 21.727901011938673 37.70991392629633 24.746511326392888 3 17.61435607870179 27.604417474218106 30.26537064530026 24.515855801779843 4 12.900322522400092 17.877636124038094 37.01269079331404 32.20935056024777 5 13.352128860176366 37.83804403248684 33.20967306349477 15.600154043842018 6 29.93968540901989 38.175050470697805 17.436420475784406 14.448843644497908 7 25.658838762955682 33.1031645913885 22.855401559417267 18.382595086238553 8 27.304330357197166 31.93359295236682 20.244132721643133 20.51794396879289 9 27.33518059072845 13.581773464420946 20.54842133874256 38.534624606108046 10-11 25.390301805823057 25.334726255659 26.796451853899907 22.478520084618033 12-13 26.899921780628116 23.49562390332565 27.187261972681785 22.417192343364448 14-15 24.401785864021335 23.643764252115783 26.200709934909167 25.753739948953715 16-17 22.588546991242296 26.14531173763301 27.003207725304364 24.262933545820335 18-19 23.110913540049587 24.360346792823282 30.557481158967448 21.971258508159682 20-21 24.627936636921525 23.66999697097611 29.546416271992793 22.155650120109573 22-23 23.65531237438805 23.541710944925118 29.398901244610798 23.404075436076038 24-25 23.08800904501637 24.293284238700984 29.455389151367605 23.16331756491504 26-27 23.473544873781112 23.930600770307013 29.364918133808477 23.230936222103395 28-29 22.7533218903542 23.791270884928547 30.237834854420036 23.217572370297216 30-31 23.44806840206738 23.77623003481379 29.772029023714147 23.003672539404683 32-33 22.964093641500163 23.991033533188666 29.185270776140886 23.859602049170288 34-35 23.44121269395615 24.04011831787859 29.708607206852438 22.810061781312818 36-37 23.65605963788306 23.929358227518797 28.892169703887284 23.52241243071086 38-39 23.78795164475302 23.396985121983814 29.064787571235502 23.75027566202767 40-41 23.482685817929426 23.93099178027534 29.197930810004085 23.388391591791155 42-43 24.253044968405526 23.798230862364665 28.50825873876867 23.440465430461135 44-45 23.839015582845448 24.1881105262257 27.85624893069254 24.116624960236315 46-47 23.60513276178682 24.335495937058866 27.841386893241594 24.21798440791272 48-49 24.65747092319539 24.736454936796278 26.686621498353674 23.919452641654658 50-51 23.794398964675118 25.310301166304534 26.18153219949711 24.71376766952324 52-53 23.732375213426423 25.1891229640314 25.930643472518494 25.14785835002368 54-55 23.991407164936174 25.260712413210996 25.97712087716917 24.770759544683663 56-57 24.346786785847417 24.584348880877467 25.65558535943214 25.413278973842974 58-59 23.658058133276704 24.69630255760489 26.67063353520454 24.975005773913868 60-61 23.96591331500163 24.918196370071993 26.320723059109064 24.795167255817315 62-63 23.47950560352041 24.96132911413303 26.061335735235158 25.4978295471114 64-65 23.275241996069393 24.722960748333993 26.33764944618217 25.664147809414445 66-67 24.226152171695432 24.597629019821422 26.18540754273869 24.99081126574445 68-69 24.121455040709513 24.158323477565126 26.165798133881868 25.554423347843493 70-71 23.367411779129824 24.429795936845032 26.352959249843792 25.84983303418135 72-73 24.198095034190782 24.458611942417612 26.265599342686173 25.077693680705433 74-75 24.438766014247708 24.369322643873854 26.11120253986173 25.08070880201671 76-77 23.76344835340489 24.839168910919067 26.19689454669693 25.200488188979115 78-79 23.94746512201592 25.00071902619784 26.043936975594207 25.00787887619203 80-81 23.403718809023914 24.875690539325582 26.375676158746646 25.344914492903857 82-83 23.66802454291369 24.93757308627991 25.782849746686367 25.611552624120034 84-85 23.700061292984813 25.169763494579474 25.255664040064445 25.87451117237127 86-87 23.39756729238109 24.759915534895555 25.674036017057766 26.168481155665585 88-89 23.449788845927998 24.36165884849476 25.941556348272755 26.24699595730449 90-91 23.732088630670667 24.653294419865748 25.618276122263918 25.996340827199667 92-93 23.32730766416806 24.85042849042042 25.554844392973497 26.267419452438023 94-95 22.597608067634273 24.976298165815606 26.128957615446463 26.297136151103658 96-97 23.497502698628672 24.81850348212814 25.5114186988313 26.172575120411885 98-99 23.068762665464558 24.884917077212652 25.603497818772613 26.442822438550177 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 93.0 1 117.0 2 356.0 3 566.5 4 411.5 5 407.0 6 796.0 7 2033.5 8 3764.0 9 3833.0 10 2381.0 11 1472.5 12 1408.5 13 1614.5 14 1955.0 15 2470.5 16 3080.5 17 3710.0 18 4593.0 19 5685.5 20 6971.0 21 8727.5 22 10858.5 23 13473.0 24 16460.5 25 20115.0 26 24554.5 27 28597.5 28 33923.5 29 41851.0 30 48429.5 31 55658.5 32 63776.0 33 70345.0 34 76708.5 35 83060.0 36 87684.5 37 95394.5 38 106384.5 39 106092.0 40 104627.0 41 111812.5 42 123118.5 43 142245.5 44 164765.5 45 191435.0 46 225371.0 47 253666.5 48 268569.5 49 286760.0 50 312018.0 51 293221.5 52 258047.5 53 247780.0 54 231194.5 55 221499.5 56 202916.5 57 181883.5 58 174804.0 59 153470.5 60 123167.0 61 92583.0 62 75163.5 63 60010.0 64 42848.0 65 33970.0 66 24461.5 67 18009.0 68 14461.0 69 12378.5 70 11614.0 71 11002.5 72 12014.5 73 10529.0 74 6554.5 75 4978.0 76 3978.0 77 2630.5 78 1308.0 79 853.0 80 713.5 81 560.0 82 503.0 83 401.5 84 270.5 85 200.5 86 94.5 87 34.5 88 22.5 89 17.0 90 11.0 91 6.5 92 2.5 93 2.0 94 1.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05651397408803008 2 0.007350987404439336 3 0.0 4 0.0 5 0.002137521160156119 6 0.0 7 0.0 8 0.0 9 0.0037015610334410845 10-11 0.0 12-13 0.0 14-15 0.002424261803591696 16-17 0.004370622534790764 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.562535266926865E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 6.951288325710955E-5 54-55 0.0 56-57 7.646417158282051E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0014684596588064394 70-71 1.3033665610708044E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.214970850641317E-4 80-81 7.038179429782343E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.9969907872838E-4 92-93 0.0010079368072280887 94-95 4.7790107239262827E-4 96-97 3.0411886424985433E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 5754329.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.43264377854436 #Duplication Level Percentage of deduplicated Percentage of total 1 71.01958244288639 23.03352818671097 2 15.151222050392008 9.827883751399808 3 5.512969671268127 5.364005445304739 4 2.6043698081764597 3.3786639302473205 5 1.3855285382105091 2.2468176762394365 6 0.8852224425272284 1.7226062485955134 7 0.577759613930666 1.3116790223769823 8 0.3944294990977891 1.0233913151990621 9 0.29232060393158726 0.8532657014797912 >10 1.7477654145729062 10.581425578648092 >50 0.180894197721148 4.123990930031702 >100 0.1910271932688458 13.44436246079091 >500 0.03464469365070882 7.713714858638716 >1k 0.021449299986563083 12.669067932807923 >5k 5.430202528243794E-4 1.1309277653820882 >10k+ 2.715101264121897E-4 1.574669196146973 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 22434 0.389863005747499 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 21189 0.3682271208337236 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20920 0.363552379434683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14329 0.24901252604778074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10539 0.1831490691616694 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 8569 0.14891397415754296 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7574 0.13162264444733696 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 7257 0.126113748449211 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 7175 0.12468873434244028 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 6354 0.11042121505391853 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 5888 0.10232296415446528 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 5851 0.10167996998433702 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.737822081427739E-5 0.0 4 0.0 1.737822081427739E-5 0.0 3.475644162855478E-5 0.0 5 0.0 1.737822081427739E-5 0.0 5.213466244283217E-5 0.0 6 0.0 1.737822081427739E-5 0.0 5.213466244283217E-5 0.0 7 0.0 1.737822081427739E-5 0.0 6.951288325710956E-5 0.0 8 0.0 1.737822081427739E-5 0.0 6.951288325710956E-5 0.0 9 0.0 1.737822081427739E-5 0.0 6.951288325710956E-5 0.0 10-11 0.0 1.737822081427739E-5 0.0 1.2164754569994173E-4 0.0 12-13 0.0 1.737822081427739E-5 0.0 1.2164754569994173E-4 0.0 14-15 1.737822081427739E-5 1.737822081427739E-5 0.0 1.477148769213578E-4 8.689110407138695E-6 16-17 3.475644162855478E-5 1.737822081427739E-5 0.0 1.9116042895705128E-4 1.737822081427739E-5 18-19 3.475644162855478E-5 1.737822081427739E-5 0.0 2.2591687058560607E-4 1.737822081427739E-5 20-21 4.344555203569347E-5 1.737822081427739E-5 0.0 6.169268389068474E-4 1.737822081427739E-5 22-23 5.213466244283217E-5 1.737822081427739E-5 0.0 0.0020766973873061483 1.737822081427739E-5 24-25 6.0823772849970866E-5 5.213466244283217E-5 0.0 0.0052569117963189105 1.737822081427739E-5 26-27 1.3033665610708044E-4 5.213466244283217E-5 0.0 0.008141696451488958 1.737822081427739E-5 28-29 1.5640398732849652E-4 5.213466244283217E-5 0.0 0.01903784090204088 1.737822081427739E-5 30-31 1.824713185499126E-4 5.213466244283217E-5 0.0 0.051830543578582314 1.737822081427739E-5 32-33 1.9116042895705128E-4 5.213466244283217E-5 0.0 0.1059028776422064 1.737822081427739E-5 34-35 1.9984953936418997E-4 5.213466244283217E-5 0.0 0.17691028788934382 1.737822081427739E-5 36-37 2.0853864977132868E-4 5.213466244283217E-5 0.0 0.2886174912835189 1.737822081427739E-5 38-39 2.172277601784674E-4 6.0823772849970866E-5 0.0 0.45724705695485957 1.737822081427739E-5 40-41 2.2591687058560607E-4 6.951288325710956E-5 0.0 0.6642477341841246 1.737822081427739E-5 42-43 2.519842018070222E-4 9.558021447852564E-5 0.0 0.8471187518127656 1.737822081427739E-5 44-45 2.7805153302843825E-4 1.0426932488566434E-4 0.0 1.0599150656835923 1.737822081427739E-5 46-47 2.7805153302843825E-4 1.0426932488566434E-4 0.0 1.286752981972355 1.737822081427739E-5 48-49 2.867406434355769E-4 1.1295843529280304E-4 0.0 1.5302566120220098 1.737822081427739E-5 50-51 2.954297538427156E-4 1.3033665610708044E-4 0.0 1.813017642891117 1.737822081427739E-5 52-53 2.954297538427156E-4 1.477148769213578E-4 0.0 2.0870895633530857 1.737822081427739E-5 54-55 2.954297538427156E-4 1.737822081427739E-4 0.0 2.342627611316628 1.737822081427739E-5 56-57 2.954297538427156E-4 1.737822081427739E-4 0.0 2.635268160718652 1.737822081427739E-5 58-59 3.0411886424985433E-4 1.824713185499126E-4 0.0 2.954132445329421 1.737822081427739E-5 60-61 3.1280797465699304E-4 1.9116042895705128E-4 0.0 3.2769589642858445 1.737822081427739E-5 62-63 3.1280797465699304E-4 2.172277601784674E-4 0.0 3.600680461614204 1.737822081427739E-5 64-65 3.1280797465699304E-4 2.2591687058560607E-4 0.0 3.941171942028341 1.737822081427739E-5 66-67 3.301861954712704E-4 2.2591687058560607E-4 0.0 4.308260789398729 1.737822081427739E-5 68-69 3.301861954712704E-4 2.4329509139988346E-4 0.0 4.6959428284340365 1.737822081427739E-5 70-71 3.301861954712704E-4 2.7805153302843825E-4 0.0 5.141138089254195 1.737822081427739E-5 72-73 3.649426370998252E-4 3.1280797465699304E-4 0.0 5.568981196591297 1.737822081427739E-5 74-75 3.649426370998252E-4 3.1280797465699304E-4 0.0 5.9820788835674845 1.737822081427739E-5 76-77 3.649426370998252E-4 3.1280797465699304E-4 0.0 6.426935269081764 1.737822081427739E-5 78-79 3.649426370998252E-4 3.649426370998252E-4 0.0 6.881775442453847 1.737822081427739E-5 80-81 3.649426370998252E-4 3.910099683212413E-4 0.0 7.381764233501421 1.737822081427739E-5 82-83 3.649426370998252E-4 4.1707729954265735E-4 0.0 7.918056127830022 1.737822081427739E-5 84-85 3.649426370998252E-4 4.1707729954265735E-4 0.0 8.438290546126229 1.737822081427739E-5 86-87 3.649426370998252E-4 4.1707729954265735E-4 0.0 8.972974955029509 1.737822081427739E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 460 0.0 42.458385 2 GTATCAA 56975 0.0 27.145735 1 TCACGCA 760 0.0 25.698051 4 AGTACTT 34790 0.0 23.272285 12-13 GTACTAG 2355 0.0 22.523657 1 ATCACGC 920 0.0 22.23972 3 CAACGCA 69365 0.0 22.189732 5 ATCAACG 69830 0.0 21.982038 3 AACGCAG 72210 0.0 21.669289 6 TATCAAC 72220 0.0 21.467434 2 TCAACGC 72500 0.0 21.255875 4 TACTTTT 40015 0.0 20.483261 14-15 GAGTACT 33905 0.0 20.292982 12-13 ACGCAGA 75900 0.0 20.070541 7 CGCAGAG 76370 0.0 19.953283 8 TAGGCAT 3495 0.0 19.824562 5 TAACGCA 505 0.0 19.337147 4 GTATAAG 2455 0.0 19.33186 1 GCAGAGT 78730 0.0 19.314493 9 CTAGGCA 3725 0.0 18.725328 4 >>END_MODULE