FastQCFastQC Report
Fri 27 May 2016
ERR765415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6214482
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89040.14327823300477818No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT86550.13927146301172003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19200.021.0701661
GTATCAA216300.020.5733641
GTACAAG35250.020.1828371
GTACATG329100.018.6648651
TACATGG327150.017.9089452
TAGGCAT31350.017.7987755
TAGTACT20400.017.3232694
CATGGGG139400.017.0452964
GTATTAG23550.016.9807721
CTAGGCA33800.016.9213434
CAACGCA243600.016.6832475
ACATGGG324750.016.5664943
AACGCAG245000.016.5499556
ATCAACG247250.016.4933823
GTATAAG24300.016.4566751
TATCAAC269000.016.4216862
GTACATA16450.016.3950211
TCTAACG8650.016.1269262
GTATATA16300.015.6900741
TCAACGC260400.015.6783354
Produced by FastQC (version 0.11.5)