FastQCFastQC Report
Fri 27 May 2016
ERR765415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6214482
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146320.2354500342908709No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100780.16216959032144596No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT92810.1493447080545088No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89250.14361615336563852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75450.12140995822338854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA295300.033.3081631
TATCACG5400.031.0013222
ATCAACG380550.025.4780273
CAACGCA380850.025.3846975
TATCAAC394850.024.8144042
TCAACGC395950.024.534074
AACGCAG400650.024.4071836
GGTATCA193300.024.3107681
ACGCAGA432700.022.3629327
CGCAGAG439900.021.9651998
AGTACTT248750.021.53700612-13
GCAGAGT466000.020.615559
AGAGTAC446150.019.29349910-11
GAGTACT236200.019.18659412-13
TACTTTT283300.018.96805614-15
TCACGCA9650.018.3116634
ATCACGC9200.018.1964283
GTACTAG17150.017.6343081
GTACTTT267200.016.9955514-15
GTATAAC10000.016.7498781
Produced by FastQC (version 0.11.5)