##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765415_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6214482 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34274184075197 33.0 33.0 33.0 33.0 33.0 2 32.30473094941783 33.0 33.0 33.0 33.0 33.0 3 32.33684480862605 33.0 33.0 33.0 33.0 33.0 4 36.20800028063481 37.0 37.0 37.0 33.0 37.0 5 36.18492048090251 37.0 37.0 37.0 33.0 37.0 6 36.209610229782626 37.0 37.0 37.0 33.0 37.0 7 36.104715887824604 37.0 37.0 37.0 33.0 37.0 8 36.106110211599294 37.0 37.0 37.0 33.0 37.0 9 36.12554722984152 37.0 37.0 37.0 33.0 37.0 10-11 36.08433285348642 37.0 37.0 37.0 33.0 37.0 12-13 36.06856307573182 37.0 37.0 37.0 33.0 37.0 14-15 37.56884097500001 40.0 37.0 40.0 33.0 40.0 16-17 37.566773546049376 40.0 37.0 40.0 33.0 40.0 18-19 37.605907298468324 40.0 37.0 40.0 33.0 40.0 20-21 37.58749554669238 40.0 37.0 40.0 33.0 40.0 22-23 37.56169178702264 40.0 37.0 40.0 33.0 40.0 24-25 37.5450003717124 40.0 37.0 40.0 33.0 40.0 26-27 37.42928461294119 40.0 37.0 40.0 33.0 40.0 28-29 37.32895557505839 38.5 37.0 40.0 33.0 40.0 30-31 37.18273284885208 37.0 37.0 40.0 33.0 40.0 32-33 37.07888372675309 37.0 37.0 40.0 33.0 40.0 34-35 36.98887719040783 37.0 37.0 40.0 33.0 40.0 36-37 36.84975738927235 37.0 37.0 40.0 33.0 40.0 38-39 36.73860790006311 37.0 37.0 40.0 33.0 40.0 40-41 36.58074172553722 37.0 37.0 40.0 33.0 40.0 42-43 36.433420516786434 37.0 37.0 40.0 33.0 40.0 44-45 36.16489443528842 37.0 37.0 40.0 33.0 40.0 46-47 35.94486668398106 37.0 37.0 40.0 33.0 40.0 48-49 35.68714753699504 37.0 37.0 40.0 27.0 40.0 50-51 35.49598478199792 37.0 37.0 40.0 27.0 40.0 52-53 35.339431508531206 37.0 35.0 40.0 27.0 40.0 54-55 35.14819336511072 37.0 33.0 40.0 27.0 40.0 56-57 34.953572719335256 37.0 33.0 40.0 27.0 40.0 58-59 34.74323925952316 37.0 33.0 40.0 27.0 40.0 60-61 34.58063729205427 37.0 33.0 37.0 27.0 40.0 62-63 34.36123694621692 37.0 33.0 37.0 27.0 40.0 64-65 34.128559548486905 37.0 33.0 37.0 27.0 40.0 66-67 33.897835813186035 37.0 33.0 37.0 27.0 40.0 68-69 33.68548585384912 37.0 33.0 37.0 27.0 40.0 70-71 33.49254523546774 37.0 33.0 37.0 27.0 37.0 72-73 33.28181286871536 37.0 33.0 37.0 27.0 37.0 74-75 33.050606711870756 37.0 33.0 37.0 27.0 37.0 76-77 32.88312670307839 37.0 33.0 37.0 27.0 37.0 78-79 32.719062988677095 35.0 33.0 37.0 27.0 37.0 80-81 32.549674293046465 33.0 33.0 37.0 24.5 37.0 82-83 32.45489068919984 33.0 33.0 37.0 22.0 37.0 84-85 32.32904657218413 33.0 33.0 37.0 22.0 37.0 86-87 32.225165991308685 33.0 33.0 37.0 22.0 37.0 88-89 32.1116051184958 33.0 33.0 37.0 22.0 37.0 90-91 32.01496641232528 33.0 33.0 37.0 22.0 37.0 92-93 31.943285538521152 33.0 33.0 37.0 22.0 37.0 94-95 31.842185398557756 33.0 33.0 37.0 22.0 37.0 96-97 31.786748034671273 33.0 33.0 37.0 22.0 37.0 98-99 30.98034075889189 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.015403166409647895 1101 2 0.07616663705472604 1101 3 0.10019345013736825 1101 4 0.11385395928802922 1101 5 0.11112110981411405 1101 6 0.14726855828845942 1101 7 0.0803798638349491 1101 8 0.14346532185295757 1101 9 0.09617192258699703 1101 10-11 0.08476631640223786 1101 12-13 0.09549473519515317 1101 14-15 0.1684645111905283 1101 16-17 0.1892953189644686 1101 18-19 0.16402068175788997 1101 20-21 0.23155621269119564 1101 22-23 0.1712017852357235 1101 24-25 0.22147102898824045 1101 26-27 0.2243456239605237 1101 28-29 0.2085487400120627 1101 30-31 0.1601269458323955 1101 32-33 0.20863155780354958 1101 34-35 0.2511503940375519 1101 36-37 0.25951693054213365 1101 38-39 0.22007156702771624 1101 40-41 0.14126350787694975 1101 42-43 0.18257468145814215 1101 44-45 0.25693659131878377 1101 46-47 0.25458308247926453 1101 48-49 0.20209294723764515 1101 50-51 0.219035173261787 1101 52-53 0.26355075973180675 1101 54-55 0.19416228789685874 1101 56-57 0.28176183311128966 1101 58-59 0.2750488136981062 1101 60-61 0.14195789518422686 1101 62-63 0.184381361596337 1101 64-65 0.1311051274716064 1101 66-67 0.22612535965367897 1101 68-69 0.32601126166412087 1101 70-71 0.19726124391927158 1101 72-73 0.14519241372822478 1101 74-75 0.23736416046955355 1101 76-77 0.25164609485106837 1101 78-79 0.19293539332664977 1101 80-81 0.21559790031335524 1101 82-83 0.2285467691034384 1101 84-85 0.16929433203691957 1101 86-87 0.20304863583623955 1101 88-89 0.19109798631221508 1101 90-91 0.16245539773189677 1101 92-93 0.22573948585419146 1101 94-95 0.23636701047913533 1101 96-97 0.21612587857663712 1101 98-99 0.31481092388516885 1102 1 -0.007317685733781332 1102 2 0.14245002143391616 1102 3 0.06207521876113731 1102 4 0.13007780050105566 1102 5 0.11236838571537078 1102 6 0.1516290203154398 1102 7 0.09620550708003606 1102 8 0.18297367605097037 1102 9 0.13733604572830416 1102 10-11 0.0836667471392829 1102 12-13 0.059869622060034544 1102 14-15 0.16666989712164337 1102 16-17 0.13506798110147855 1102 18-19 0.16777145953413708 1102 20-21 0.2124547875695555 1102 22-23 0.1711531050332269 1102 24-25 0.2178043608603346 1102 26-27 0.2210151619565579 1102 28-29 0.1442761219778177 1102 30-31 0.18150276315056857 1102 32-33 0.197854253584417 1102 34-35 0.3008857862794301 1102 36-37 0.24893759616981725 1102 38-39 0.2651193242159309 1102 40-41 0.19538141353032046 1102 42-43 0.22702143878876058 1102 44-45 0.3117280660321313 1102 46-47 0.3091138704186207 1102 48-49 0.28537088089539253 1102 50-51 0.3155442697235742 1102 52-53 0.3217696694050858 1102 54-55 0.21458623705539992 1102 56-57 0.2286463023654619 1102 58-59 0.3596620018607126 1102 60-61 0.25886304447476505 1102 62-63 0.34269806451052176 1102 64-65 0.2052221942424879 1102 66-67 0.3492185956970104 1102 68-69 0.3053912688339224 1102 70-71 0.27342567436300413 1102 72-73 0.20531493241610832 1102 74-75 0.4169389294082322 1102 76-77 0.4461226680446657 1102 78-79 0.31047303654552394 1102 80-81 0.30812417577783435 1102 82-83 0.353681977743193 1102 84-85 0.27778340770133525 1102 86-87 0.30121041271909377 1102 88-89 0.4398091703404958 1102 90-91 0.3320744757838838 1102 92-93 0.33445432806546904 1102 94-95 0.32979835579602224 1102 96-97 0.4321660933057565 1102 98-99 0.4157491881560418 1103 1 -0.005870569989085084 1103 2 0.07559461617020702 1103 3 0.06152709790851674 1103 4 0.08932680063587384 1103 5 0.029773619466624268 1103 6 0.0689190935639914 1103 7 0.049791510465347244 1103 8 0.18313772883786328 1103 9 0.05659403341535807 1103 10-11 0.07963792705510286 1103 12-13 0.0939030857128813 1103 14-15 0.1658924738528711 1103 16-17 0.16083751030353 1103 18-19 0.2529837005802875 1103 20-21 0.15702825331948844 1103 22-23 0.14840692848773074 1103 24-25 0.17234933606967928 1103 26-27 0.2199657154399901 1103 28-29 0.2068878447417859 1103 30-31 0.23843465248384632 1103 32-33 0.17659108307557148 1103 34-35 0.24477188403403716 1103 36-37 0.1898458781992005 1103 38-39 0.08625247480236453 1103 40-41 0.14583291819636202 1103 42-43 0.1682793366002997 1103 44-45 0.16485360715767428 1103 46-47 0.1169066773809817 1103 48-49 0.09604845245877414 1103 50-51 0.14637552843026924 1103 52-53 0.06054538503893525 1103 54-55 0.05366411419555561 1103 56-57 0.0038963210895275324 1103 58-59 0.15326861669916525 1103 60-61 0.08343231603365098 1103 62-63 0.11180443219128477 1103 64-65 0.10540560022520395 1103 66-67 0.17859584820229202 1103 68-69 0.1684306193334777 1103 70-71 -0.04696786059108149 1103 72-73 0.07634355776998802 1103 74-75 0.10926266103992077 1103 76-77 0.16659056001428496 1103 78-79 0.14301546528172082 1103 80-81 0.14934372669981144 1103 82-83 0.1004299517991214 1103 84-85 0.0865619094763801 1103 86-87 0.1048473755256083 1103 88-89 0.18702791238276006 1103 90-91 0.12648343936931639 1103 92-93 0.11358277516623616 1103 94-95 0.0788794984134924 1103 96-97 0.10875238129688469 1103 98-99 0.13407834227758642 1104 1 -0.04737395651562082 1104 2 0.03472123376545255 1104 3 0.005302557391949847 1104 4 0.0048620214640635595 1104 5 -0.03552641494957243 1104 6 0.011307893200516617 1104 7 0.07672134359440719 1104 8 0.100027331745693 1104 9 0.037867127975559356 1104 10-11 0.0035704604272410734 1104 12-13 -0.005522503885543983 1104 14-15 0.08314791140787037 1104 16-17 0.06388345524577943 1104 18-19 -0.02807697667060438 1104 20-21 0.07109457455455015 1104 22-23 0.08333718059657969 1104 24-25 0.09538603080888919 1104 26-27 0.07410560299560842 1104 28-29 0.05138684966480156 1104 30-31 0.01980834028133671 1104 32-33 0.08762498098869997 1104 34-35 0.008080855778750617 1104 36-37 0.08301020125438185 1104 38-39 0.047446311446506684 1104 40-41 0.06630107097161897 1104 42-43 0.09497028595679069 1104 44-45 0.08387130633290951 1104 46-47 0.07101677384155636 1104 48-49 0.05353903017476114 1104 50-51 -0.05993599936319072 1104 52-53 0.059318307518154256 1104 54-55 -0.019217283238049276 1104 56-57 0.1219859691601215 1104 58-59 0.04128097388215224 1104 60-61 0.08682874572734534 1104 62-63 0.03185254412962024 1104 64-65 0.063986314368087 1104 66-67 0.11035079400018333 1104 68-69 0.06023874291619791 1104 70-71 -0.013492012463089509 1104 72-73 0.10057772938030496 1104 74-75 0.0189916436944344 1104 76-77 0.008310259817989163 1104 78-79 0.12703750797140856 1104 80-81 0.11287409933335368 1104 82-83 0.12159990115426211 1104 84-85 0.2033477577540026 1104 86-87 0.208580240544741 1104 88-89 0.1312231841686895 1104 90-91 0.14105404902571905 1104 92-93 0.16666496185981572 1104 94-95 0.1510749824099662 1104 96-97 0.1806989858602286 1104 98-99 0.21008389239940328 1105 1 -0.0569874531059682 1105 2 0.08878942136501422 1105 3 0.02828034466176632 1105 4 0.030937190246262958 1105 5 0.023332061025065798 1105 6 0.012191820836719103 1105 7 -0.009533164859327314 1105 8 0.06999487169500185 1105 9 0.0021524749738048854 1105 10-11 0.02280675822392908 1105 12-13 -0.04620081039101365 1105 14-15 0.033217149177538374 1105 16-17 0.05205828952431801 1105 18-19 0.03610058369717706 1105 20-21 0.053911370202605724 1105 22-23 -0.00504761696680589 1105 24-25 0.09593375165408702 1105 26-27 0.015030650504918697 1105 28-29 0.01177096162490443 1105 30-31 -0.005980931931738098 1105 32-33 0.013578096062580869 1105 34-35 0.06170694896910334 1105 36-37 0.13041730677063157 1105 38-39 0.09565507220496272 1105 40-41 -0.02658266621922678 1105 42-43 -0.04626611794515867 1105 44-45 0.01768477598884033 1105 46-47 -0.0181582576839574 1105 48-49 0.03449001090033477 1105 50-51 0.038011892066627695 1105 52-53 -0.07545461496106753 1105 54-55 -0.08810211957067793 1105 56-57 -0.01610367891046849 1105 58-59 0.05856731799786985 1105 60-61 0.02680893941027307 1105 62-63 -0.06289686651001603 1105 64-65 -2.0478938518664336E-4 1105 66-67 0.10051792612436827 1105 68-69 0.028742307645167386 1105 70-71 -0.005149678772902178 1105 72-73 -0.04542267599624239 1105 74-75 -0.033594481817218025 1105 76-77 -0.00821463479090312 1105 78-79 -0.015322197055937181 1105 80-81 -0.03216276680668528 1105 82-83 -0.024089528720359965 1105 84-85 -0.12793159701713108 1105 86-87 0.0023019209801589113 1105 88-89 0.027287652642499438 1105 90-91 0.04804188092776229 1105 92-93 0.03997238555584204 1105 94-95 0.022360017894015982 1105 96-97 -0.035507358962853175 1105 98-99 0.12659782279706633 1106 1 -0.01656147054818291 1106 2 0.054790599821124886 1106 3 0.014428302226676237 1106 4 -0.005460935955191815 1106 5 0.05575690245339615 1106 6 0.036279101242620015 1106 7 0.004610985385831157 1106 8 0.07126977991140393 1106 9 0.014475233307642554 1106 10-11 0.0052511641567392076 1106 12-13 0.012279615772840202 1106 14-15 -0.02429501264954581 1106 16-17 0.033597779802043704 1106 18-19 0.020657630838080365 1106 20-21 -0.015357987005572227 1106 22-23 0.0231586555125034 1106 24-25 0.012721232290878959 1106 26-27 -0.01694254361773062 1106 28-29 -0.020288094490403807 1106 30-31 -0.02572177290148403 1106 32-33 -0.07355887946444994 1106 34-35 -0.0065077433391991235 1106 36-37 -0.00755298120458292 1106 38-39 -0.1326991098478416 1106 40-41 -0.11461813721507497 1106 42-43 -0.14448269791620305 1106 44-45 -0.14391696983257418 1106 46-47 -0.06915200013530409 1106 48-49 -0.06197801214621279 1106 50-51 -0.09164189778958587 1106 52-53 -0.11969764887233936 1106 54-55 -0.1819191282651218 1106 56-57 -0.1521396884767654 1106 58-59 -0.03427305452224516 1106 60-61 -0.12262276212571521 1106 62-63 -0.18028641024486092 1106 64-65 -0.1508923998654481 1106 66-67 -0.10363025540053172 1106 68-69 -0.06618632110649258 1106 70-71 -0.16282095883752845 1106 72-73 -0.10930060713921108 1106 74-75 -0.10199004847597593 1106 76-77 -0.013695516873312386 1106 78-79 -0.07726144524359313 1106 80-81 -0.04194006526269334 1106 82-83 -0.17950889645998558 1106 84-85 -0.11267148615693401 1106 86-87 -0.06590878270745293 1106 88-89 -0.01463482520811965 1106 90-91 -0.1746773505695458 1106 92-93 -0.05788237106097682 1106 94-95 -0.10962136676526057 1106 96-97 -0.12462488729778798 1106 98-99 -0.05743135598965665 1107 1 -0.05384767869900031 1107 2 0.015444115423235871 1107 3 0.030154342443246662 1107 4 -0.004864871761519396 1107 5 0.003649746180244051 1107 6 -0.055109689176902066 1107 7 2.741404342856413E-4 1107 8 0.003553018153944265 1107 9 0.015565379780497324 1107 10-11 -0.07350548866895679 1107 12-13 -0.0521902184266807 1107 14-15 0.02941698950763083 1107 16-17 -0.014441782933154457 1107 18-19 -0.04882999677936084 1107 20-21 -0.05782489515508615 1107 22-23 0.021746121654075523 1107 24-25 0.059956695640515534 1107 26-27 0.04251927468252603 1107 28-29 -0.025081317360061917 1107 30-31 -0.035697747070337016 1107 32-33 0.05510018768571712 1107 34-35 -0.015189315650836477 1107 36-37 0.0570027472988599 1107 38-39 -0.020535830825863854 1107 40-41 -0.04035037548297993 1107 42-43 0.05230804556631341 1107 44-45 -0.038356775075229166 1107 46-47 0.0750018100835419 1107 48-49 -0.020171581108790804 1107 50-51 -0.041936470789828206 1107 52-53 -0.09059624427919744 1107 54-55 -0.10644976259512617 1107 56-57 -3.3899441663010066E-4 1107 58-59 0.02061899729847738 1107 60-61 -0.03866257426574293 1107 62-63 -0.07379250931314374 1107 64-65 -0.035492366486515436 1107 66-67 -0.043388016178411704 1107 68-69 -0.10626164194550825 1107 70-71 -0.05430523573369328 1107 72-73 -0.11545267602258491 1107 74-75 0.05277197380060983 1107 76-77 -0.03239949355153726 1107 78-79 0.025089224348789685 1107 80-81 -0.07978763210167017 1107 82-83 -0.09836929885891976 1107 84-85 0.023928415155729965 1107 86-87 0.016018472637057357 1107 88-89 -0.00919774388428607 1107 90-91 -0.008803152310456142 1107 92-93 0.0505112319802663 1107 94-95 -0.005805485500946617 1107 96-97 0.0768269940446622 1107 98-99 0.017497091108385376 1108 1 -0.05355029826891666 1108 2 0.006396809415377902 1108 3 -0.030523059739209657 1108 4 -0.04150551352741161 1108 5 0.013248820393421568 1108 6 0.04036878123061172 1108 7 -0.08190925506410451 1108 8 -0.11441618405107334 1108 9 -0.06796107088226933 1108 10-11 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68-69 -0.050989234293552954 2215 70-71 -0.17324155650842954 2215 72-73 -0.19134765985216973 2215 74-75 -0.2982538417942564 2215 76-77 -0.16357288979557438 2215 78-79 -0.11681717445423345 2215 80-81 -0.1970843424685924 2215 82-83 -0.1599759102945839 2215 84-85 -0.16416758754593985 2215 86-87 -0.22536626442560248 2215 88-89 -0.16596900230475597 2215 90-91 -0.25242174108558757 2215 92-93 -0.08438113385292567 2215 94-95 -0.09015483465675089 2215 96-97 -0.13622013789662546 2215 98-99 -0.26117425871258604 2216 1 -0.16272284084826083 2216 2 -0.13769129239763345 2216 3 -0.183224385360802 2216 4 -0.24397357447808332 2216 5 -0.1444046717473384 2216 6 -0.25898095575377766 2216 7 -0.13953397187739824 2216 8 -0.2100962316780297 2216 9 -0.14708549074287447 2216 10-11 -0.1645490022411451 2216 12-13 -0.14892386869060203 2216 14-15 -0.3867086649392988 2216 16-17 -0.4139033621726682 2216 18-19 -0.32025898768656447 2216 20-21 -0.361794098092048 2216 22-23 -0.3969746266659655 2216 24-25 -0.46683850485809586 2216 26-27 -0.43531920677244074 2216 28-29 -0.3761852800620602 2216 30-31 -0.38473142884564027 2216 32-33 -0.3589811375691454 2216 34-35 -0.3808172242109933 2216 36-37 -0.3967303028119602 2216 38-39 -0.4103460337086773 2216 40-41 -0.366521093729574 2216 42-43 -0.48395493775596066 2216 44-45 -0.5119110872963191 2216 46-47 -0.4209048091761929 2216 48-49 -0.30277192624192395 2216 50-51 -0.3531514394235131 2216 52-53 -0.37574795108409376 2216 54-55 -0.32824971420848925 2216 56-57 -0.4433308544966792 2216 58-59 -0.5349278174765573 2216 60-61 -0.4927328536643216 2216 62-63 -0.40264274548719214 2216 64-65 -0.43144834444922253 2216 66-67 -0.4450000449724314 2216 68-69 -0.30047090951741495 2216 70-71 -0.41006077228239235 2216 72-73 -0.42051803863130743 2216 74-75 -0.374707270064647 2216 76-77 -0.33594126489268206 2216 78-79 -0.2729634924693869 2216 80-81 -0.44203959193782794 2216 82-83 -0.4174774204603793 2216 84-85 -0.43212366731737006 2216 86-87 -0.5160035801930078 2216 88-89 -0.5316580230856829 2216 90-91 -0.5054167295066847 2216 92-93 -0.3032009888258145 2216 94-95 -0.4128419309051736 2216 96-97 -0.40489471269082955 2216 98-99 -0.43938947414074647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 19.0 10 48.0 11 114.0 12 255.0 13 459.0 14 931.0 15 2490.0 16 5602.0 17 10784.0 18 17774.0 19 25543.0 20 33668.0 21 43348.0 22 52106.0 23 51151.0 24 43880.0 25 42185.0 26 49243.0 27 63769.0 28 85359.0 29 112834.0 30 150005.0 31 199957.0 32 266054.0 33 354140.0 34 474378.0 35 675363.0 36 1123008.0 37 1714227.0 38 615155.0 39 630.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.760294611324134 18.85522134893602 13.029297709911372 25.35518632982847 2 16.620548583132113 21.044601947515496 36.67700702970899 25.657842439643403 3 18.243515710561233 25.61106460683288 29.522267503550577 26.62315217905531 4 13.693208862782127 17.211587385722574 35.90598540634601 33.18921834514928 5 14.552585449313431 36.69947817741389 33.178225738838314 15.569710634434369 6 32.12250031458777 36.48806127365081 17.01049258811917 14.378945823642262 7 27.63367888103948 31.6680778864594 22.100924904119122 18.597318328381995 8 27.27429253154165 32.39433310773126 20.037325717573886 20.294048643153204 9 27.248771498573817 14.16227450654777 19.665854692313857 38.92309930256456 10-11 25.733271091621152 24.717136520791275 27.169633768349478 22.379958619238096 12-13 26.911478704097945 22.66530018109313 27.10558981424357 23.317631300565356 14-15 24.347990361743577 23.431538545462622 25.178569072293683 27.041902020500125 16-17 23.768670680009144 25.84619053638299 25.810135949368128 24.57500283423974 18-19 24.068266810846993 24.62064964823862 28.18977252978547 23.12131101112892 20-21 25.111000991797933 24.075860674556154 27.487808094380995 23.325330239264915 22-23 24.498566413097663 23.745832717835533 27.499613000729582 24.25598786833722 24-25 24.121222010136968 24.268635744700845 27.501584202834607 24.108558042327584 26-27 24.290512065205114 24.240588354749438 27.42330736495817 24.045592215087275 28-29 23.97613348948472 24.136589340833233 27.86496927660262 24.02230789307942 30-31 24.35629048566261 24.061212958115405 27.577338271165104 24.00515828505688 32-33 24.109901678048146 24.134545727222317 27.225487176565967 24.530065418163574 34-35 24.477768219458998 24.113618802017612 27.514497588053192 23.894115390470194 36-37 24.557549607513547 24.08310137514277 27.082715824102475 24.276633193241206 38-39 24.505831700856163 23.801050513944684 27.260840082890255 24.432277702308898 40-41 24.389908925635314 24.00679573937136 27.328029914641316 24.27526542035201 42-43 24.808133646537232 24.002539551969093 26.91204190469938 24.277284896794292 44-45 24.480495719514515 24.30960456559372 26.482754314840722 24.727145400051043 46-47 24.407770430423646 24.356543312861795 26.462261858671404 24.77342439804315 48-49 25.070842589937502 24.528094216058555 25.779791461299588 24.62127173270435 50-51 24.418262053056072 24.925778206453895 25.586943529645755 25.069016210844282 52-53 24.494961929248486 24.779989707911295 25.462178504982397 25.262869857857822 54-55 24.582772948734906 24.752425061332545 25.5408254461112 25.123976543821353 56-57 24.58764865679875 24.491687320037293 25.421668290293542 25.498995732870416 58-59 24.286024505825626 24.464768299656214 25.907414802023304 25.341792392494856 60-61 24.34091047331057 24.61466619422182 25.788609573573467 25.255813758894146 62-63 24.089940239588753 24.6392700147816 25.63505695245396 25.63573279317568 64-65 24.07038913299612 24.311720590710536 25.73232974204447 25.885560534248874 66-67 24.441562466509676 24.373061181929565 25.715956695988503 25.469419655572256 68-69 24.374702509396602 24.09587798307244 25.729787293615143 25.799632213915817 70-71 23.867902425334886 24.194599002780922 25.797765606208205 26.139732965675982 72-73 24.280881334920593 24.247821459616425 25.732852714031516 25.738444491431466 74-75 24.37608204967786 24.15352920172391 25.67253827714278 25.79785047145545 76-77 24.127176943573115 24.3082783092475 25.662648440725206 25.90189630645418 78-79 24.1160824490611 24.36719760681486 25.637424662837816 25.87929528128623 80-81 23.835954777562698 24.230597516462154 25.764796306819804 26.168651399155348 82-83 23.879311260375363 24.324070775327694 25.47772284158197 26.318895122714974 84-85 23.871389910431446 24.314252345095905 25.293565424370072 26.520792320102576 86-87 23.690825435184653 24.121234912737787 25.46468051540036 26.723259136677196 88-89 23.68775488923904 23.862926875109423 25.62505792937251 26.82426030627903 90-91 23.86711608402816 23.968605902098773 25.412393906974213 26.751884106898853 92-93 23.56197475163055 23.983207339298367 25.439659602488195 27.015158306582883 94-95 23.1282646383283 23.982544772728755 25.812814033053964 27.07637655588898 96-97 23.619355676519056 23.86521353837875 25.48722210144467 27.028208683657525 98-99 23.272950886676266 23.857894608109753 25.520392492071696 27.348762013142284 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 77.0 1 96.5 2 291.0 3 480.0 4 363.0 5 325.5 6 641.5 7 1692.5 8 3079.5 9 3106.0 10 2003.0 11 1367.0 12 1345.5 13 1511.5 14 1809.0 15 2217.5 16 2661.5 17 3121.0 18 3713.0 19 4413.5 20 5396.0 21 6666.5 22 8185.5 23 10189.5 24 12716.0 25 15918.5 26 19959.5 27 23906.0 28 28451.0 29 34328.0 30 39414.0 31 45683.5 32 52842.5 33 59834.5 34 66809.0 35 73499.0 36 80162.0 37 89117.5 38 101189.5 39 107344.0 40 112188.5 41 121215.5 42 135522.0 43 156277.5 44 175957.0 45 196734.5 46 226347.5 47 251842.0 48 267760.0 49 280776.5 50 294414.0 51 294658.5 52 290699.0 53 291764.5 54 280842.5 55 270655.0 56 250694.0 57 230957.0 58 217002.5 59 190685.0 60 159785.0 61 126419.0 62 103580.0 63 83113.5 64 61863.0 65 47854.0 66 34893.0 67 26374.0 68 20706.0 69 17621.5 70 15819.0 71 13594.0 72 13291.5 73 11299.0 74 7499.5 75 5754.5 76 4405.0 77 2958.5 78 1695.0 79 1058.5 80 691.5 81 500.0 82 333.5 83 205.5 84 124.5 85 79.5 86 45.5 87 19.5 88 8.5 89 4.0 90 3.0 91 2.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05772001592409472 2 0.0 3 0.0 4 0.0 5 0.004843525172331338 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012873156604202892 16-17 0.004722839329166936 18-19 6.758407217206518E-4 20-21 1.6896018043016295E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.172345176959238E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.045722877626808E-6 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.827433726576085E-5 76-77 1.045943974091485E-4 78-79 4.022861438813404E-5 80-81 8.045722877626808E-6 82-83 0.0 84-85 3.861946981260868E-4 86-87 8.206637335179344E-4 88-89 2.8964602359456506E-4 90-91 0.001053989696969112 92-93 0.0016171902984029884 94-95 0.0011022640342348727 96-97 0.00191488204487518 98-99 6.114749386996373E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6214482.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.45220251200227 #Duplication Level Percentage of deduplicated Percentage of total 1 72.95249471470844 30.2404158466987 2 14.323038971222873 11.874430240448625 3 4.976867701959704 6.18906383571231 4 2.285713774890791 3.7899148112498504 5 1.3043360327099136 2.7033800685796483 6 0.8057842429283081 2.00409189713068 7 0.5710619181724315 1.6570241995283272 8 0.40161315013981663 1.3318199704863083 9 0.31819991191987107 1.1871078469383405 >10 1.7641743937382475 13.46319802257773 >50 0.14904966433577116 4.295618439556502 >100 0.12391037326482798 10.490199792029237 >500 0.015603892619653191 4.440048581312486 >1k 0.007957179832495524 5.52177479195729 >5k 1.1644653412967304E-4 0.41433061271779115 >10k+ 7.763102275311535E-5 0.3975810430762541 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14632 0.2354500342908709 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10078 0.16216959032144596 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9281 0.1493447080545088 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 8925 0.14361615336563852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7545 0.12140995822338854 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.6091445755253617E-5 1.6091445755253617E-5 2 3.2182891510507234E-5 0.0 0.0 1.6091445755253617E-5 1.6091445755253617E-5 3 3.2182891510507234E-5 0.0 0.0 1.6091445755253617E-5 1.6091445755253617E-5 4 3.2182891510507234E-5 1.6091445755253617E-5 0.0 1.6091445755253617E-5 6.436578302101447E-5 5 3.2182891510507234E-5 1.6091445755253617E-5 0.0 3.2182891510507234E-5 6.436578302101447E-5 6 3.2182891510507234E-5 1.6091445755253617E-5 0.0 3.2182891510507234E-5 6.436578302101447E-5 7 4.827433726576085E-5 1.6091445755253617E-5 0.0 4.827433726576085E-5 6.436578302101447E-5 8 4.827433726576085E-5 1.6091445755253617E-5 0.0 4.827433726576085E-5 6.436578302101447E-5 9 4.827433726576085E-5 3.2182891510507234E-5 0.0 4.827433726576085E-5 6.436578302101447E-5 10-11 4.827433726576085E-5 3.2182891510507234E-5 0.0 8.045722877626809E-5 6.436578302101447E-5 12-13 4.827433726576085E-5 3.2182891510507234E-5 0.0 9.65486745315217E-5 1.1264012028677531E-4 14-15 4.827433726576085E-5 4.0228614388134045E-5 0.0 9.65486745315217E-5 1.6091445755253615E-4 16-17 4.827433726576085E-5 4.827433726576085E-5 0.0 1.2873156604202893E-4 1.7700590330778978E-4 18-19 4.827433726576085E-5 8.045722877626808E-5 0.0 1.2873156604202893E-4 2.2528024057355062E-4 20-21 4.827433726576085E-5 8.045722877626808E-5 0.0 2.655088549616847E-4 2.4137168632880424E-4 22-23 4.827433726576085E-5 8.045722877626808E-5 0.0 0.0013034071061755429 2.5746313208405787E-4 24-25 8.850295165389489E-5 8.850295165389489E-5 0.0 0.003491843728890035 2.7355457783931146E-4 26-27 1.1264012028677531E-4 9.65486745315217E-5 0.0 0.0060342921582201055 2.8964602359456506E-4 28-29 1.1264012028677531E-4 9.65486745315217E-5 0.0 0.017555767318981695 2.8964602359456506E-4 30-31 1.1264012028677531E-4 9.65486745315217E-5 0.0 0.05172595238026275 2.8964602359456506E-4 32-33 1.1264012028677531E-4 9.65486745315217E-5 0.0 0.11270448606979633 3.057374693498187E-4 34-35 1.1264012028677531E-4 1.2873156604202893E-4 0.0 0.19569611755251684 3.1378319222744554E-4 36-37 1.1264012028677531E-4 1.5286873467490936E-4 0.0 0.3213864003468029 3.379203608603259E-4 38-39 1.1264012028677531E-4 1.930973490630434E-4 0.0 0.5082644056254407 3.4596608373795273E-4 40-41 1.1264012028677531E-4 2.09188794818297E-4 0.0 0.7259092551881234 3.5401180661557956E-4 42-43 1.1264012028677531E-4 2.172345176959238E-4 0.0 0.9285729687526652 3.5401180661557956E-4 44-45 1.1264012028677531E-4 2.3332596345117745E-4 0.0 1.1729618011605796 3.5401180661557956E-4 46-47 1.1264012028677531E-4 2.4137168632880424E-4 0.0 1.4415441222615175 3.5401180661557956E-4 48-49 1.1264012028677531E-4 2.4941740920643104E-4 0.0 1.7178744744936103 3.7010325237083315E-4 50-51 1.1264012028677531E-4 2.5746313208405787E-4 0.0 2.0204741119211547 3.7010325237083315E-4 52-53 1.1264012028677531E-4 2.655088549616847E-4 0.0 2.3199278717035465 4.1837758963659405E-4 54-55 1.1264012028677531E-4 3.057374693498187E-4 0.0 2.6247642200910715 4.5860620402472807E-4 56-57 1.1264012028677531E-4 3.057374693498187E-4 0.0 2.9745359307501413 4.6665192690235484E-4 58-59 1.1264012028677531E-4 3.218289151050723E-4 0.0 3.347945975223679 4.6665192690235484E-4 60-61 1.1264012028677531E-4 3.298746379826991E-4 0.0 3.727840872336584 4.827433726576085E-4 62-63 1.1264012028677531E-4 3.379203608603259E-4 0.0 4.111911499622977 4.988348184128621E-4 64-65 1.1264012028677531E-4 3.620575294932064E-4 0.0 4.518928528556362 5.068805412904889E-4 66-67 1.1264012028677531E-4 3.7010325237083315E-4 0.0 4.960711126043973 5.149262641681157E-4 68-69 1.1264012028677531E-4 3.7010325237083315E-4 0.0 5.431715789023124 5.310177099233693E-4 70-71 1.1264012028677531E-4 3.942404210037136E-4 0.0 5.947856957345761 5.310177099233693E-4 72-73 1.1264012028677531E-4 4.264233125142208E-4 0.0 6.462525758381792 5.390634328009961E-4 74-75 1.1264012028677531E-4 4.3446903539184765E-4 0.0 6.971844153704202 5.471091556786229E-4 76-77 1.2873156604202893E-4 4.425147582694744E-4 0.0 7.513329349091364 5.953834929443838E-4 78-79 1.2873156604202893E-4 4.5056048114710124E-4 0.0 8.084205570150498 6.27566384454891E-4 80-81 1.2873156604202893E-4 4.6665192690235484E-4 0.0 8.69592992625934 6.27566384454891E-4 82-83 1.3677728891965573E-4 4.6665192690235484E-4 0.0 9.337383228401016 6.27566384454891E-4 84-85 1.4482301179728253E-4 4.6665192690235484E-4 0.0 9.964305955025697 6.27566384454891E-4 86-87 1.4482301179728253E-4 4.827433726576085E-4 0.0 10.608229294090803 6.27566384454891E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 29530 0.0 33.308163 1 TATCACG 540 0.0 31.001322 2 ATCAACG 38055 0.0 25.478027 3 CAACGCA 38085 0.0 25.384697 5 TATCAAC 39485 0.0 24.814404 2 TCAACGC 39595 0.0 24.53407 4 AACGCAG 40065 0.0 24.407183 6 GGTATCA 19330 0.0 24.310768 1 ACGCAGA 43270 0.0 22.362932 7 CGCAGAG 43990 0.0 21.965199 8 AGTACTT 24875 0.0 21.537006 12-13 GCAGAGT 46600 0.0 20.61555 9 AGAGTAC 44615 0.0 19.293499 10-11 GAGTACT 23620 0.0 19.186594 12-13 TACTTTT 28330 0.0 18.968056 14-15 TCACGCA 965 0.0 18.311663 4 ATCACGC 920 0.0 18.196428 3 GTACTAG 1715 0.0 17.634308 1 GTACTTT 26720 0.0 16.99555 14-15 GTATAAC 1000 0.0 16.749878 1 >>END_MODULE