FastQCFastQC Report
Fri 27 May 2016
ERR765414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12621198
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA245460.19448233044121485No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT244880.19402278610952778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG267650.020.1880724
GTATCAA507250.019.813991
GTACATG797700.018.6253931
TACATGG790800.018.3477782
TATACCG18200.017.81325
ACATGGG760500.017.7194843
GTATAAG38750.017.5824551
CAACGCA563150.017.037115
AACGCAG572950.016.8677716
ATCAACG574950.016.6631953
GTATTAT29050.016.6599731
GTACTAG31800.016.4013291
GTATAAA78100.016.3644371
TCAACGC588850.016.2935354
TATCAAC625150.016.0617622
ATGGGGG124950.015.75585655
ACGCAGA605300.015.6868527
CGCAGAG616150.015.34198958
TAATACG22350.014.9260564
GTATTAG54300.014.7106641
Produced by FastQC (version 0.11.5)