##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765414_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12621198
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9365442171179	33.0	33.0	33.0	27.0	33.0
2	31.8370068356427	33.0	33.0	33.0	27.0	33.0
3	31.856069051448205	33.0	33.0	33.0	27.0	33.0
4	35.64760128158991	37.0	37.0	37.0	33.0	37.0
5	35.68134609725638	37.0	37.0	37.0	33.0	37.0
6	35.66854374679804	37.0	37.0	37.0	33.0	37.0
7	35.61058260871908	37.0	37.0	37.0	33.0	37.0
8	35.613459910857905	37.0	37.0	37.0	33.0	37.0
9	35.61699333137789	37.0	37.0	37.0	33.0	37.0
10-11	35.5766829741519	37.0	37.0	37.0	33.0	37.0
12-13	35.529876007016135	37.0	37.0	37.0	33.0	37.0
14-15	37.10636415021776	40.0	37.0	40.0	33.0	40.0
16-17	37.12781159918416	40.0	37.0	40.0	33.0	40.0
18-19	37.082814008622634	40.0	37.0	40.0	33.0	40.0
20-21	37.169711702486566	40.0	37.0	40.0	33.0	40.0
22-23	37.142730072058136	40.0	37.0	40.0	33.0	40.0
24-25	37.11555107526243	40.0	37.0	40.0	33.0	40.0
26-27	37.06504279546205	40.0	37.0	40.0	33.0	40.0
28-29	37.00730497215874	40.0	37.0	40.0	33.0	40.0
30-31	36.93699674943694	37.0	37.0	40.0	33.0	40.0
32-33	36.833679180058816	37.0	37.0	40.0	33.0	40.0
34-35	36.72804229836185	37.0	37.0	40.0	33.0	40.0
36-37	36.65314033580647	37.0	37.0	40.0	33.0	40.0
38-39	36.55879818223278	37.0	37.0	40.0	33.0	40.0
40-41	36.468964554711846	37.0	37.0	40.0	33.0	40.0
42-43	36.33801969511927	37.0	37.0	40.0	33.0	40.0
44-45	36.20358657712208	37.0	37.0	40.0	33.0	40.0
46-47	36.04061024951831	37.0	37.0	40.0	27.0	40.0
48-49	35.81629984728866	37.0	37.0	40.0	27.0	40.0
50-51	35.72641947301675	37.0	37.0	40.0	27.0	40.0
52-53	35.575811147246085	37.0	35.0	40.0	27.0	40.0
54-55	35.413928852078854	37.0	33.0	40.0	27.0	40.0
56-57	35.250559851766845	37.0	33.0	40.0	27.0	40.0
58-59	35.08179302788848	37.0	33.0	40.0	27.0	40.0
60-61	34.8862587767025	37.0	33.0	40.0	27.0	40.0
62-63	34.661471557612835	37.0	33.0	37.0	27.0	40.0
64-65	34.46086080734967	37.0	33.0	37.0	27.0	40.0
66-67	34.255783840804966	37.0	33.0	37.0	27.0	40.0
68-69	34.048497654501574	37.0	33.0	37.0	27.0	40.0
70-71	33.82857918875847	37.0	33.0	37.0	27.0	38.5
72-73	33.606154978315054	37.0	33.0	37.0	27.0	37.0
74-75	33.382747065690594	37.0	33.0	37.0	27.0	37.0
76-77	33.18710727777189	37.0	33.0	37.0	27.0	37.0
78-79	33.01705614633413	37.0	33.0	37.0	27.0	37.0
80-81	32.81564028232502	37.0	33.0	37.0	27.0	37.0
82-83	32.65996484644326	35.0	33.0	37.0	24.5	37.0
84-85	32.493296198982065	33.0	33.0	37.0	22.0	37.0
86-87	32.35080144531446	33.0	33.0	37.0	22.0	37.0
88-89	32.196750855188235	33.0	33.0	37.0	22.0	37.0
90-91	32.04142772342213	33.0	33.0	37.0	22.0	37.0
92-93	31.908009564543715	33.0	33.0	37.0	22.0	37.0
94-95	31.76219602925174	33.0	33.0	37.0	22.0	37.0
96-97	31.647248264388217	33.0	33.0	37.0	22.0	37.0
98-99	31.4994331758364	33.0	33.0	37.0	22.0	37.0
100	31.41002573606721	33.0	33.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	14.0
7	247.0
8	1432.0
9	2872.0
10	4173.0
11	5784.0
12	9095.0
13	15843.0
14	22761.0
15	25502.0
16	29169.0
17	33704.0
18	40766.0
19	47955.0
20	56034.0
21	67220.0
22	78762.0
23	84209.0
24	89801.0
25	101639.0
26	121516.0
27	149793.0
28	187604.0
29	241362.0
30	312770.0
31	405875.0
32	530981.0
33	692954.0
34	900227.0
35	1226990.0
36	2014541.0
37	3475270.0
38	1642604.0
39	1727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.261004317140134	18.541537595445345	12.63530982878044	25.562148258634082
2	16.50620246412415	20.881792973129045	36.88936099675543	25.722643565991373
3	18.565469649222518	25.31517296806588	28.968711181557616	27.150646201153982
4	13.517820034069397	17.007464951855724	35.87803412977288	33.596680884301996
5	14.813023665202413	36.63852489079529	33.17873284247261	15.369718601529685
6	32.392368775135296	36.27372773963295	17.20674218089281	14.127161304338939
7	27.92755489613585	31.26072501199965	22.099772145243264	18.711947946621233
8	26.876030310276413	32.54842369163371	20.388096280559104	20.187449717530775
9	27.089338111960526	14.57197644787761	19.518733483144786	38.819951957017075
10-11	25.751208403512884	24.974099130684742	27.14628595478813	22.128406511014248
12-13	26.56545361224822	22.896998367349912	26.696550517629152	23.840997502772716
14-15	24.37478993673976	23.884250132198225	24.15568633025169	27.585273600810318
16-17	24.26503096708191	26.17044065102181	25.041596863168664	24.52293151872762
18-19	24.66508733832571	25.608607796369487	26.400372966371776	23.32593189893302
20-21	25.798480079362214	24.950548166507733	25.353444280438037	23.897527473692012
22-23	24.99229729505407	24.688669878646095	25.293844773132808	25.025188053167025
24-25	24.634248741401894	25.266742055839202	25.37542021117341	24.723588991585498
26-27	24.847571278735714	25.177443599847837	25.235230584171504	24.739754537244952
28-29	24.4765532442709	25.051782304667096	25.78871107916847	24.682953371893532
30-31	24.960383083389384	24.960010619192012	25.52478917634892	24.554817121069686
32-33	24.42654815861514	25.061922699471918	25.24520160739303	25.26632753451991
34-35	24.89636785184027	24.964460798831777	25.694152045694622	24.445019303633327
36-37	24.94409054803387	24.889952162967948	25.310503290460044	24.855453998538138
38-39	24.93057259943019	24.51436085067278	25.511977301402528	25.0430892484945
40-41	24.722688189168153	24.824781083597014	25.728396126295287	24.72413460093955
42-43	25.16116304370351	24.625097338527997	25.576719758271977	24.63701985949652
44-45	24.62352880670584	24.78662293716355	25.403065814635013	25.1867824414956
46-47	24.303437510413435	24.70425645565631	25.922158359976212	25.070147673954047
48-49	24.78780139951152	24.99015020033209	25.42039151012247	24.80165689003392
50-51	24.10124069675533	25.21097391624752	25.291655853739226	25.396129533257923
52-53	24.129252254914018	24.957032840018716	25.330632751762277	25.583082153304986
54-55	24.284745941095732	24.882931120853634	25.467794061483122	25.364528876567512
56-57	24.230719079559908	24.48939752384568	25.255048158098774	26.024835238495637
58-59	24.041126478251996	24.507543860156566	25.582396686247854	25.868932975343583
60-61	24.081322820014613	24.546698913174147	25.478497137777744	25.893481129033496
62-63	23.652205894793717	24.615655948462468	25.40765287966158	26.324485277082232
64-65	23.693673919279355	24.319851630213428	25.511294931306466	26.475179519200747
66-67	24.036285033523182	24.45871496746737	25.302556550001675	26.202443449007777
68-69	24.122574603914785	24.19733947056839	25.16181060848126	26.51827531703556
70-71	23.554385498098547	24.133351579383433	25.467098750459883	26.84516417205814
72-73	23.935236232717227	24.152988165879748	25.3766858837238	26.535089717679227
74-75	23.943261614507136	24.037411986338707	25.5051475472808	26.514178851873353
76-77	23.624357397173345	24.281443883490006	25.573248055615988	26.52095066372066
78-79	23.73752190539074	24.29101174667355	25.450239894064136	26.521226453871577
80-81	23.44213344202093	24.09746006657855	25.797590849831252	26.662815641569264
82-83	23.52863459229764	24.23500344817532	25.3731429610952	26.863218998431844
84-85	23.477810787436855	24.19421062786009	25.23498550220527	27.09299308249778
86-87	23.364758596982394	23.946931979039682	25.509982591173298	27.17832683280463
88-89	23.404769573359417	23.742697217227256	25.632584306608525	27.2199489028048
90-91	23.682217412635108	23.761192378977153	25.42270523745655	27.133884970931188
92-93	23.482192536789253	23.80963379213722	25.457817767430864	27.250355903642664
94-95	23.10449082159824	23.76087564989455	25.790537404629145	27.344096123878064
96-97	23.485241498236256	23.63875135701265	25.52806872443326	27.34793842031783
98-99	23.394925598748177	23.615396201730185	25.560660732779617	27.429017466742025
100	23.36881413463847	23.664917981349998	25.3946506234495	27.571617260562036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	485.0
1	458.5
2	652.0
3	863.0
4	709.5
5	602.0
6	807.5
7	1537.0
8	2343.0
9	2256.5
10	1543.0
11	1170.5
12	1235.0
13	1400.5
14	1634.5
15	1899.0
16	2180.0
17	2559.5
18	3124.5
19	3834.0
20	4684.0
21	6064.5
22	7928.0
23	10119.0
24	13091.5
25	17271.0
26	23407.0
27	31038.5
28	39417.5
29	51344.0
30	65424.5
31	79240.0
32	94783.0
33	111479.5
34	129021.5
35	144057.0
36	159244.0
37	179565.0
38	197559.5
39	213568.5
40	230144.0
41	247644.0
42	267612.0
43	302051.5
44	343945.5
45	378902.0
46	424240.0
47	476223.0
48	530875.5
49	576232.5
50	616094.0
51	622897.5
52	592935.0
53	580875.0
54	563940.0
55	538833.5
56	518079.0
57	484796.0
58	454450.5
59	418329.0
60	362048.5
61	300251.0
62	245982.5
63	191805.0
64	143332.5
65	116808.5
66	95522.5
67	71593.5
68	54018.0
69	43432.5
70	37164.5
71	32183.0
72	28218.5
73	28409.5
74	22923.5
75	16905.0
76	13308.0
77	9856.5
78	7567.5
79	5252.0
80	3878.5
81	3089.0
82	2546.5
83	2214.0
84	1652.0
85	1023.0
86	706.5
87	403.0
88	192.0
89	125.0
90	97.5
91	71.0
92	47.5
93	39.5
94	22.5
95	11.5
96	11.0
97	10.5
98	7.5
99	1.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01739929917904782
2	0.005783920036750869
3	0.005918614065003972
4	0.003858587750544758
5	0.04493234318960847
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.2785161915691365E-4
18-19	0.0017510223672903315
20-21	6.219694834040319E-4
22-23	0.005003486990696129
24-25	0.007182360977143374
26-27	0.0056175332959676255
28-29	0.02176100874100858
30-31	0.020089218155043602
32-33	0.012942511479496639
34-35	0.013330747207990873
36-37	0.013358478331454749
38-39	0.020766649885375382
40-41	0.02985057361432726
42-43	0.01760926339956001
44-45	0.016678289968987098
46-47	0.01809257726564467
48-49	0.013089090274948543
50-51	0.010086205762717612
52-53	0.027113115569536266
54-55	0.025346246845980865
56-57	0.013227745892267913
58-59	0.016072166841848135
60-61	0.017090295231878938
62-63	0.020509146596067982
64-65	0.010918139466633833
66-67	0.015414543056847693
68-69	0.007649828486962965
70-71	0.008834343617777013
72-73	0.010561596450669739
74-75	0.003406966596990238
76-77	0.007459672211782115
78-79	0.005795804803949672
80-81	0.002503724289881198
82-83	2.3769534397606313E-4
84-85	0.002479954755483592
86-87	0.0023888382069594347
88-89	0.001212246254277922
90-91	8.71549594578898E-4
92-93	0.0012320541996092605
94-95	0.003173232842080443
96-97	0.001461826365452788
98-99	0.0019094859299410407
100	0.002281875302170206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2621198E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.063787901589215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.81587265181854	32.64918659451811
2	13.060131485886062	11.24837464550129
3	4.44118372385864	5.737625817487159
4	2.0412490471798117	3.5161566408828997
5	1.1074480653941927	2.384545430008041
6	0.696506378452444	1.7996521772268046
7	0.49150650145375885	1.4816292211599775
8	0.3538158974924261	1.2189322212659413
9	0.25299256049237956	0.9805336169151432
>10	1.4852525442183946	11.878034756100659
>50	0.13323974817345466	3.9999220141798673
>100	0.09688695849599027	8.582318879573208
>500	0.013196450709205935	3.8980502387786733
>1k	0.009943195263070456	8.13627779243039
>5k	7.19448889474098E-4	2.0116182721789153
>10k+	5.534222226723831E-5	0.47714168179294697
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	24546	0.19448233044121485	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	24488	0.19402278610952778	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.923178132535437E-6
2	0.0	0.0	0.0	0.0	7.923178132535437E-6
3	0.0	0.0	0.0	0.0	7.923178132535437E-6
4	0.0	0.0	0.0	0.0	7.923178132535437E-6
5	0.0	0.0	0.0	0.0	7.923178132535437E-6
6	0.0	0.0	0.0	0.0	1.5846356265070874E-5
7	0.0	0.0	0.0	0.0	2.376953439760631E-5
8	0.0	0.0	0.0	0.0	2.376953439760631E-5
9	0.0	0.0	0.0	7.923178132535437E-6	4.753906879521262E-5
10-11	0.0	0.0	0.0	1.5846356265070874E-5	4.753906879521262E-5
12-13	3.9615890662677186E-6	0.0	0.0	2.376953439760631E-5	8.319337039162209E-5
14-15	1.1884767198803156E-5	7.923178132535437E-6	0.0	3.961589066267719E-5	1.2280926105429928E-4
16-17	1.5846356265070874E-5	7.923178132535437E-6	0.0	3.961589066267719E-5	1.465787954519056E-4
18-19	1.5846356265070874E-5	1.1884767198803156E-5	0.0	3.961589066267719E-5	1.5450197358444102E-4
20-21	2.376953439760631E-5	1.5846356265070874E-5	0.0	9.507813759042526E-5	1.8619468611458278E-4
22-23	2.376953439760631E-5	2.376953439760631E-5	0.0	4.951986332834648E-4	2.2581057677725997E-4
24-25	3.565430159640947E-5	4.35774797289449E-5	0.0	0.0017549839563565995	2.2977216584352768E-4
26-27	5.5462246927748063E-5	4.753906879521262E-5	0.0	0.0039497042990689155	2.4165693304233085E-4
28-29	5.5462246927748063E-5	4.753906879521262E-5	0.0	0.01976436785160965	2.53541700241134E-4
30-31	5.5462246927748063E-5	4.753906879521262E-5	0.0	0.07659732459628635	2.53541700241134E-4
32-33	5.5462246927748063E-5	4.753906879521262E-5	0.0	0.1998740531604052	2.693880565062049E-4
34-35	5.5462246927748063E-5	5.546224692774806E-5	0.0	0.367853352748289	2.891960018375435E-4
36-37	5.5462246927748063E-5	6.33854250602835E-5	0.0	0.6347733392662092	2.9711917997007886E-4
38-39	6.33854250602835E-5	6.33854250602835E-5	0.0	1.0600657718863138	3.288118925002207E-4
40-41	6.33854250602835E-5	7.130860319281894E-5	0.0	1.5641264799110195	3.3277348156648837E-4
42-43	6.33854250602835E-5	7.527019225908666E-5	0.0	2.005657466113756	3.406966596990238E-4
44-45	6.33854250602835E-5	7.923178132535438E-5	0.0	2.520307501712595	3.6842778316289787E-4
46-47	6.33854250602835E-5	7.923178132535438E-5	0.0	3.1110319321509734	3.961589066267719E-4
48-49	6.33854250602835E-5	7.923178132535438E-5	0.0	3.7145839879859266	4.040820847593073E-4
50-51	6.33854250602835E-5	9.111654852415753E-5	0.0	4.3388789241718575	4.357747972894491E-4
52-53	6.33854250602835E-5	9.507813759042525E-5	0.0	4.9492369900226585	4.5162115355451993E-4
54-55	6.33854250602835E-5	9.903972665669297E-5	0.0	5.563540798583462	4.555827426207876E-4
56-57	6.33854250602835E-5	1.0300131572296069E-4	0.0	6.253146492115883	4.674675098195908E-4
58-59	6.734701412655121E-5	1.0300131572296069E-4	0.0	7.005654296842502	4.833138660846617E-4
60-61	7.527019225908666E-5	1.0300131572296069E-4	0.0	7.762733775351595	4.991602223497326E-4
62-63	7.923178132535438E-5	1.0300131572296069E-4	0.0	8.50715993838303	5.150065786148035E-4
64-65	7.923178132535438E-5	1.0300131572296069E-4	0.0	9.245976491296627	5.268913458136065E-4
66-67	7.923178132535438E-5	1.1092449385549613E-4	0.0	10.034962608145438	5.308529348798743E-4
68-69	7.923178132535438E-5	1.1092449385549613E-4	0.0	10.914110530553438	5.308529348798743E-4
70-71	7.923178132535438E-5	1.1092449385549613E-4	0.0	11.876594440559446	5.427377020786775E-4
72-73	7.923178132535438E-5	1.1488608292176385E-4	0.0	12.788873132328643	5.744304146088192E-4
74-75	8.715495945788982E-5	1.1884767198803157E-4	0.0	13.662439175742271	5.902767708738902E-4
76-77	8.715495945788982E-5	1.1884767198803157E-4	0.0	14.57818821953352	6.100847162052287E-4
78-79	8.715495945788982E-5	1.2280926105429928E-4	0.0	15.533259996396538	6.180078943377641E-4
80-81	8.715495945788982E-5	1.3073243918683473E-4	0.0	16.55536978343894	6.33854250602835E-4
82-83	8.715495945788982E-5	1.3865561731937016E-4	0.0	17.600571673148618	6.536621959341736E-4
84-85	8.715495945788982E-5	1.4261720638563788E-4	0.0	18.59590111810305	6.655469631329767E-4
86-87	8.715495945788982E-5	1.505403845181733E-4	0.0	19.58601315025721	6.972396756631184E-4
88	8.715495945788982E-5	1.505403845181733E-4	0.0	20.344344490911244	7.210092100607248E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	26765	0.0	20.188072	4
GTATCAA	50725	0.0	19.81399	1
GTACATG	79770	0.0	18.625393	1
TACATGG	79080	0.0	18.347778	2
TATACCG	1820	0.0	17.8132	5
ACATGGG	76050	0.0	17.719484	3
GTATAAG	3875	0.0	17.582455	1
CAACGCA	56315	0.0	17.03711	5
AACGCAG	57295	0.0	16.867771	6
ATCAACG	57495	0.0	16.663195	3
GTATTAT	2905	0.0	16.659973	1
GTACTAG	3180	0.0	16.401329	1
GTATAAA	7810	0.0	16.364437	1
TCAACGC	58885	0.0	16.293535	4
TATCAAC	62515	0.0	16.061762	2
ATGGGGG	12495	0.0	15.7558565	5
ACGCAGA	60530	0.0	15.686852	7
CGCAGAG	61615	0.0	15.3419895	8
TAATACG	2235	0.0	14.926056	4
GTATTAG	5430	0.0	14.710664	1
>>END_MODULE