FastQCFastQC Report
Fri 27 May 2016
ERR765410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742887
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA505620.6530122420745647No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT446210.5762837556585806No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG204920.2646558060320395No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG146760.18954170453475558No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC138350.17868012280174048No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC131710.17010451011360492No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG128080.16541633630969946No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT111380.14384815379586452No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC89420.11548663954413901No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT80470.10392764352624544No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA22550.021.858741
GTACTAG22950.021.4777581
GTATAAG29300.021.266841
ATACGGG16750.021.0991333
TACGGGC17050.020.4529064
CGGGCGT18800.017.8061646
GTACAAG42700.017.4244041
TGATCGC20850.017.3949629
TAGTACT30400.016.9772574
GTGATCG21400.016.9461448
GGCGTCG20250.016.7605368
CATGGGG185750.016.4708184
ATAAGGT28600.016.2592243
TACATGA28750.016.1743932
GTATTAT18400.016.174391
GTACAAA42450.015.9934061
CCGTCGT33050.015.897989
CGTTAAC6500.015.7389281
TAAGGTG29400.015.0243044
CTAGTAC35450.014.8227233
Produced by FastQC (version 0.11.5)