##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765410_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7742887 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33167021551522 33.0 33.0 33.0 27.0 33.0 2 31.287421603853964 33.0 33.0 33.0 27.0 33.0 3 31.28733391046518 33.0 33.0 33.0 27.0 33.0 4 34.80405435336974 37.0 37.0 37.0 33.0 37.0 5 34.97887493385865 37.0 37.0 37.0 33.0 37.0 6 34.95129271549488 37.0 37.0 37.0 33.0 37.0 7 34.86933827137087 37.0 37.0 37.0 33.0 37.0 8 34.78686115398559 37.0 37.0 37.0 33.0 37.0 9 34.6687363511827 37.0 37.0 37.0 27.0 37.0 10-11 34.708560773261965 37.0 37.0 37.0 30.0 37.0 12-13 34.62904069244456 37.0 37.0 37.0 27.0 37.0 14-15 36.07314261463457 37.0 37.0 40.0 27.0 40.0 16-17 36.13745990352177 37.0 37.0 40.0 27.0 40.0 18-19 36.14733767908533 37.0 37.0 40.0 27.0 40.0 20-21 36.15654361222113 37.0 37.0 40.0 27.0 40.0 22-23 36.18873212278572 37.0 37.0 40.0 27.0 40.0 24-25 36.27829819807522 37.0 37.0 40.0 33.0 40.0 26-27 36.23727538578311 37.0 37.0 40.0 27.0 40.0 28-29 36.24138728874644 37.0 37.0 40.0 30.0 40.0 30-31 36.151326565917856 37.0 37.0 40.0 27.0 40.0 32-33 36.04605433348052 37.0 37.0 40.0 27.0 40.0 34-35 35.91254631508893 37.0 37.0 40.0 27.0 40.0 36-37 35.714587465889664 37.0 37.0 40.0 27.0 40.0 38-39 35.41327078646505 37.0 33.0 40.0 27.0 40.0 40-41 35.51051274543978 37.0 33.0 40.0 27.0 40.0 42-43 35.417539995094856 37.0 33.0 40.0 27.0 40.0 44-45 35.32223381278844 37.0 33.0 40.0 27.0 40.0 46-47 35.21409843641009 37.0 33.0 40.0 27.0 40.0 48-49 35.076981944848214 37.0 33.0 40.0 27.0 40.0 50-51 34.90193812721276 37.0 33.0 40.0 27.0 40.0 52-53 34.76044013040614 37.0 33.0 40.0 27.0 40.0 54-55 34.55843104774743 37.0 33.0 40.0 27.0 40.0 56-57 34.33856002289585 37.0 33.0 40.0 27.0 40.0 58-59 34.12440308117631 37.0 33.0 37.0 27.0 40.0 60-61 33.89509868864159 37.0 33.0 37.0 27.0 40.0 62-63 33.63868218663142 37.0 33.0 37.0 27.0 40.0 64-65 33.422006998164896 37.0 33.0 37.0 22.0 40.0 66-67 33.20015789976013 37.0 33.0 37.0 22.0 40.0 68-69 32.98666550344852 37.0 33.0 37.0 22.0 40.0 70-71 32.698006764143656 37.0 33.0 37.0 22.0 37.0 72-73 32.337720930190514 33.0 33.0 37.0 22.0 37.0 74-75 32.100965376351226 33.0 33.0 37.0 22.0 37.0 76-77 31.896088952350716 33.0 33.0 37.0 22.0 37.0 78-79 31.640435925256305 33.0 33.0 37.0 22.0 37.0 80-81 31.439800619588016 33.0 27.0 37.0 22.0 37.0 82-83 31.330055249417953 33.0 27.0 37.0 22.0 37.0 84-85 31.172754813546938 33.0 27.0 37.0 22.0 37.0 86-87 30.901215657673937 33.0 27.0 37.0 18.5 37.0 88-89 30.74360467871997 33.0 27.0 37.0 15.0 37.0 90-91 30.596407128245573 33.0 27.0 37.0 15.0 37.0 92-93 30.324324957861325 33.0 27.0 37.0 15.0 37.0 94-95 30.187836784909816 33.0 27.0 37.0 15.0 37.0 96-97 30.00599666506821 33.0 27.0 37.0 15.0 37.0 98-99 28.935160877331672 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28745682601017464 1101 2 0.2724223939322883 1101 3 0.3144235675566236 1101 4 0.3552572033916519 1101 5 0.284526904287965 1101 6 0.31367575019439187 1101 7 0.3202607104215289 1101 8 0.43118979604996355 1101 9 0.4381035061657883 1101 10-11 0.31713615379138815 1101 12-13 0.44419911980986626 1101 14-15 0.5972538863371426 1101 16-17 0.4898307219039353 1101 18-19 0.4517137981258941 1101 20-21 0.45736361341970877 1101 22-23 0.4110859550853476 1101 24-25 0.380244508178599 1101 26-27 0.3989913108728018 1101 28-29 0.39012333517703723 1101 30-31 0.5924932439050181 1101 32-33 0.6673666166777892 1101 34-35 0.7206493111120835 1101 36-37 0.837474042788962 1101 38-39 0.8264031965286378 1101 40-41 0.6227350654427255 1101 42-43 0.5299411314095224 1101 44-45 0.4641786201989788 1101 46-47 0.4991021821015238 1101 48-49 0.4092324821009612 1101 50-51 0.3135372178369593 1101 52-53 0.46818610378161907 1101 54-55 0.33681934764202026 1101 56-57 0.4808033212953049 1101 58-59 0.35795084918903086 1101 60-61 0.21304769394722456 1101 62-63 0.3429407694149944 1101 64-65 0.3784519152046002 1101 66-67 0.5002158208678011 1101 68-69 0.5681681485596073 1101 70-71 0.5428373386887984 1101 72-73 0.29600074542040034 1101 74-75 0.31512405726682147 1101 76-77 0.3433572899068871 1101 78-79 0.3173272464761361 1101 80-81 0.31105068264565716 1101 82-83 0.4550322571110321 1101 84-85 0.42342643890599874 1101 86-87 0.42545777542607155 1101 88-89 0.36749766572540565 1101 90-91 0.23252547017216685 1101 92-93 0.32939812789036793 1101 94-95 0.3110204732606263 1101 96-97 0.2729207276277563 1101 98-99 0.20797862757025598 1102 1 0.2043900788169708 1102 2 0.2733016959493604 1102 3 0.22596351815075622 1102 4 0.2535712374800596 1102 5 0.18685293673100034 1102 6 0.2948881722838479 1102 7 0.3424274466406274 1102 8 0.3799314544238399 1102 9 0.34483164772726127 1102 10-11 0.2603000927335586 1102 12-13 0.3675929849119939 1102 14-15 0.5108425156714489 1102 16-17 0.3577041718116263 1102 18-19 0.3518480181469954 1102 20-21 0.36959926253801 1102 22-23 0.4104187071146228 1102 24-25 0.2909216925667053 1102 26-27 0.3890524299179461 1102 28-29 0.27750839262684934 1102 30-31 0.4933228787659445 1102 32-33 0.5859933381005078 1102 34-35 0.5972461142881471 1102 36-37 0.7142910074112834 1102 38-39 0.7237747192491426 1102 40-41 0.5221571108516301 1102 42-43 0.3855239198325151 1102 44-45 0.32444202111140186 1102 46-47 0.30573272878364577 1102 48-49 0.1983865951133339 1102 50-51 0.23984293848103988 1102 52-53 0.3572593592908859 1102 54-55 0.23275077665710597 1102 56-57 0.3203914265601142 1102 58-59 0.10016546087368994 1102 60-61 0.11903390390200741 1102 62-63 0.1870838374014383 1102 64-65 0.26940684149519 1102 66-67 0.24749398904334186 1102 68-69 0.3705539712106045 1102 70-71 0.40099962404985945 1102 72-73 0.22273521762194548 1102 74-75 0.27049410482874237 1102 76-77 0.27965422380171745 1102 78-79 0.25883614856547155 1102 80-81 0.24711142692885701 1102 82-83 0.15499996935357885 1102 84-85 0.24454705178362346 1102 86-87 0.23241553706702334 1102 88-89 0.08751444042852441 1102 90-91 0.15920322790126207 1102 92-93 0.17961382504091006 1102 94-95 0.19868849091176344 1102 96-97 0.08728164279344952 1102 98-99 0.1829458284344483 1103 1 0.17371403642579963 1103 2 0.22775140459417287 1103 3 0.15920190360041175 1103 4 0.1930558980241841 1103 5 0.18094020314674708 1103 6 0.2597159086912484 1103 7 0.10966888855576684 1103 8 0.25168638549806843 1103 9 0.13760240800918666 1103 10-11 0.2300053320241915 1103 12-13 0.21134660998136212 1103 14-15 0.4203413159736229 1103 16-17 0.17348526587540647 1103 18-19 0.1567686761338294 1103 20-21 0.21705742286822272 1103 22-23 0.17470534263981108 1103 24-25 0.12974850678381955 1103 26-27 0.2894964234973756 1103 28-29 0.257860485174227 1103 30-31 0.3613713776923362 1103 32-33 0.45167590496947696 1103 34-35 0.4770443010416656 1103 36-37 0.5991619322010067 1103 38-39 0.6351589882506445 1103 40-41 0.42017755951532365 1103 42-43 0.3175303492810784 1103 44-45 0.2200275065610242 1103 46-47 0.2075671849039935 1103 48-49 0.1937249675842878 1103 50-51 0.1260597877924141 1103 52-53 0.22089469700147646 1103 54-55 0.10910939331251512 1103 56-57 0.16597512974943385 1103 58-59 0.13322865605226752 1103 60-61 0.043732947804400624 1103 62-63 0.14590879170791027 1103 64-65 0.2701266380069214 1103 66-67 0.21590274859386938 1103 68-69 0.32898123372904564 1103 70-71 0.29088007949502526 1103 72-73 0.07546729324154455 1103 74-75 0.19380641710441182 1103 76-77 0.12770003084948556 1103 78-79 0.09517686496755573 1103 80-81 0.06711255101004099 1103 82-83 0.15375166610605717 1103 84-85 0.19815573693773203 1103 86-87 0.125205269916556 1103 88-89 0.08069580464155379 1103 90-91 0.045969065151314936 1103 92-93 0.02973402037959616 1103 94-95 0.07844713515153146 1103 96-97 -0.052010400523609945 1103 98-99 0.02169193805972469 1104 1 0.11088867328017571 1104 2 0.08814776854996609 1104 3 0.06062598808843944 1104 4 0.09793211798096735 1104 5 0.08504886054738847 1104 6 0.15872410391743585 1104 7 0.05005989033848124 1104 8 0.13574457549869834 1104 9 0.13852923741204393 1104 10-11 0.12208262085788846 1104 12-13 0.10627041340147514 1104 14-15 0.2114888305472178 1104 16-17 0.11359630606295212 1104 18-19 0.14863512745471752 1104 20-21 0.0991090886053243 1104 22-23 0.051552792025510996 1104 24-25 0.037758768861628766 1104 26-27 0.15928846147826192 1104 28-29 9.503787466016433E-4 1104 30-31 0.23413542877916171 1104 32-33 0.27556878730847245 1104 34-35 0.44733013830392565 1104 36-37 0.44585442190305 1104 38-39 0.4898329436063946 1104 40-41 0.25942758123601095 1104 42-43 0.17416685619884475 1104 44-45 0.14419943050023676 1104 46-47 0.12323283483795677 1104 48-49 0.027026206631887817 1104 50-51 -0.05043163883363633 1104 52-53 0.06891089493908709 1104 54-55 -0.060868883961866516 1104 56-57 0.07765523274577646 1104 58-59 0.05894181899423501 1104 60-61 -0.11005874622647838 1104 62-63 0.06231307967334487 1104 64-65 0.0551204890707524 1104 66-67 0.09394819298159973 1104 68-69 0.14294091097647765 1104 70-71 0.14608836340319442 1104 72-73 0.004810647794521117 1104 74-75 0.03812224834178579 1104 76-77 0.11383683879993711 1104 78-79 0.07739668889493245 1104 80-81 -0.015614868483684319 1104 82-83 0.10415320277710904 1104 84-85 0.19301915913885992 1104 86-87 0.08372302529862097 1104 88-89 0.020357870554693847 1104 90-91 -0.05860264812361393 1104 92-93 -0.06550747389045952 1104 94-95 0.032741440472335626 1104 96-97 -0.09400813708857214 1104 98-99 -0.13792620988578363 1105 1 0.09170640879408509 1105 2 0.1430236140607164 1105 3 0.12394735530520862 1105 4 0.06932929852093395 1105 5 0.006023466252720766 1105 6 0.1673849257385811 1105 7 0.14611236287102258 1105 8 0.13606835672466389 1105 9 0.13627675345108514 1105 10-11 0.11500971619253875 1105 12-13 0.13482251711830173 1105 14-15 0.20090936101941992 1105 16-17 0.0575223288803528 1105 18-19 0.2138979712686293 1105 20-21 0.18822392114120845 1105 22-23 0.07539427795337161 1105 24-25 -0.020838393561369628 1105 26-27 0.12429589897531201 1105 28-29 0.11785554003456156 1105 30-31 0.2888398874602771 1105 32-33 0.38558290489781655 1105 34-35 0.43245644092263547 1105 36-37 0.546135125195363 1105 38-39 0.4871813570511634 1105 40-41 0.36789818212421466 1105 42-43 0.22682171538460238 1105 44-45 0.20845951897130277 1105 46-47 0.2273229681836071 1105 48-49 0.052361829650749314 1105 50-51 0.11366694661050758 1105 52-53 0.2210888341367081 1105 54-55 0.12614615346017644 1105 56-57 0.2909864178541284 1105 58-59 0.17757000807525714 1105 60-61 0.1072257092141129 1105 62-63 0.13835147502292955 1105 64-65 0.1876784637778499 1105 66-67 0.2080772243494451 1105 68-69 0.33348890636705164 1105 70-71 0.2509538157328848 1105 72-73 0.06264463464141556 1105 74-75 0.21020231611332463 1105 76-77 0.20594735335489034 1105 78-79 0.1231524159410391 1105 80-81 0.08248674503206033 1105 82-83 0.14708061907027314 1105 84-85 0.15935702697594323 1105 86-87 0.023869181350480773 1105 88-89 -0.018990689986509324 1105 90-91 -0.019431148499997164 1105 92-93 -0.004633092924859028 1105 94-95 0.0706633440165092 1105 96-97 -0.14923402797483476 1105 98-99 -0.16891038746226883 1106 1 0.11470196351641704 1106 2 0.14487481405961944 1106 3 0.06732552542276693 1106 4 -0.01604677238064056 1106 5 0.014174426439090837 1106 6 0.1643319514192072 1106 7 0.08645320060274742 1106 8 0.12155098687126298 1106 9 0.1256498864093416 1106 10-11 0.13897362933848 1106 12-13 0.13453014426210075 1106 14-15 0.26266575548752513 1106 16-17 0.09884733252253142 1106 18-19 0.20474561335561248 1106 20-21 0.11683305182876325 1106 22-23 0.065341595464524 1106 24-25 0.08936869022504368 1106 26-27 0.2161159755225981 1106 28-29 0.10314353086587147 1106 30-31 0.2903996422724049 1106 32-33 0.25020328316315243 1106 34-35 0.29171354841171393 1106 36-37 0.4454028857545822 1106 38-39 0.430754580367271 1106 40-41 0.27956855452097074 1106 42-43 0.1455063104092389 1106 44-45 0.027344867366252856 1106 46-47 0.07016849050315699 1106 48-49 -0.013405579022048641 1106 50-51 -0.08083082058910662 1106 52-53 0.068225255659641 1106 54-55 -0.08630592741934606 1106 56-57 -0.0058141616509672644 1106 58-59 0.016049247347517337 1106 60-61 -0.03090238292212888 1106 62-63 0.05330319967740138 1106 64-65 0.13155881702979855 1106 66-67 0.06568320872487021 1106 68-69 0.023435184888462857 1106 70-71 0.17648506774521877 1106 72-73 -0.04426090223769563 1106 74-75 0.07485716086542027 1106 76-77 0.00804780813554018 1106 78-79 0.04474789487420949 1106 80-81 0.09052173226961102 1106 82-83 0.11971398596335447 1106 84-85 0.16669873117944434 1106 86-87 0.041002536141046875 1106 88-89 -0.03324657123934216 1106 90-91 -0.11035249891721222 1106 92-93 -0.14219516399906595 1106 94-95 -0.017378663252308968 1106 96-97 -0.14189696211840896 1106 98-99 -0.0968828372219761 1107 1 0.03169050692524067 1107 2 0.09894199100790857 1107 3 -0.017023395361903226 1107 4 -0.027036831964387886 1107 5 -0.09055685217822429 1107 6 0.05688012242769247 1107 7 0.03855590997481784 1107 8 0.05757935949882409 1107 9 -0.09034549180058349 1107 10-11 0.06488378587324206 1107 12-13 0.020846862479416473 1107 14-15 0.07467604889387047 1107 16-17 -0.07336113362405428 1107 18-19 0.17931117260140894 1107 20-21 0.10281569274000191 1107 22-23 -0.08396410555634048 1107 24-25 -0.023819625352381024 1107 26-27 0.01475702552550473 1107 28-29 0.0455104511915394 1107 30-31 0.1816644821144635 1107 32-33 0.16435904653125988 1107 34-35 0.20951301807124167 1107 36-37 0.34011772132424056 1107 38-39 0.3133010556389948 1107 40-41 0.14225472226363678 1107 42-43 0.04244094475090776 1107 44-45 0.010786917158910114 1107 46-47 -0.08613825826188304 1107 48-49 -0.16817746022323377 1107 50-51 -0.07571304439749582 1107 52-53 -2.1476963095068413E-4 1107 54-55 -0.10674263633050884 1107 56-57 -0.04146068612352849 1107 58-59 -0.03830954736881864 1107 60-61 -0.17572047287990955 1107 62-63 -0.09659399399500757 1107 64-65 0.005848050243628222 1107 66-67 0.015559268289543127 1107 68-69 0.07400857856755039 1107 70-71 0.04740027857154416 1107 72-73 -0.28151714992477395 1107 74-75 -0.05665087258552148 1107 76-77 -0.05722126747521372 1107 78-79 -0.07479292017616501 1107 80-81 -0.10757172164906947 1107 82-83 -0.11211029803380868 1107 84-85 0.027896933518402278 1107 86-87 -0.1329174262651982 1107 88-89 -0.2733063413883734 1107 90-91 -0.2561993133390956 1107 92-93 -0.21313983709115192 1107 94-95 -0.20014247179549471 1107 96-97 -0.2698063168991496 1107 98-99 -0.30069280843552804 1108 1 -0.002558916555013724 1108 2 -0.05504451268404864 1108 3 -0.04882779205892973 1108 4 -0.18833361425924977 1108 5 -0.12899037385173528 1108 6 -0.059347002976849694 1108 7 -0.0214267036622644 1108 8 -0.13610463636909742 1108 9 -0.15547995522011604 1108 10-11 -0.18013467692416185 1108 12-13 -0.1805732236575892 1108 14-15 -0.16604965698535068 1108 16-17 -0.18262377865928414 1108 18-19 0.11023393246824043 1108 20-21 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2215 90-91 0.056666510354730804 2215 92-93 0.06997144579242942 2215 94-95 0.1934246143605094 2215 96-97 0.27527427792232473 2215 98-99 0.38953630120052196 2216 1 -0.247135377836603 2216 2 -0.21516028465122972 2216 3 -0.15148706236503884 2216 4 -0.12063744531987197 2216 5 -0.2664810377326319 2216 6 -0.22539617006822965 2216 7 -0.3717530690262194 2216 8 -0.35849692434614155 2216 9 -0.2542616172415606 2216 10-11 -0.2692607670924616 2216 12-13 -0.3707645926251075 2216 14-15 -0.4031236824908504 2216 16-17 -0.44755236604004267 2216 18-19 -0.3358521782497448 2216 20-21 -0.1140227508944136 2216 22-23 -0.1615832296952746 2216 24-25 -0.25127243636246277 2216 26-27 -0.3194361618499997 2216 28-29 -0.3467569694994168 2216 30-31 -0.26588882810289505 2216 32-33 -0.152848317306443 2216 34-35 -0.2483505474464991 2216 36-37 -0.15922931870890267 2216 38-39 -8.622845710421046 2216 40-41 -1.963334240439103 2216 42-43 -0.5948177088961657 2216 44-45 -0.5765139393076453 2216 46-47 -0.37604364092147335 2216 48-49 -0.23698569764209765 2216 50-51 -0.20603786432059223 2216 52-53 -0.20164451237996417 2216 54-55 -0.23346454599490585 2216 56-57 -0.19778291764187372 2216 58-59 -0.2665721550609348 2216 60-61 -0.21173439490986823 2216 62-63 -0.34623805676157815 2216 64-65 -0.32980109846689487 2216 66-67 -0.19139722083662747 2216 68-69 -0.1837725240740511 2216 70-71 -0.041385549993051995 2216 72-73 -0.046329551128636126 2216 74-75 -0.13133089279897092 2216 76-77 -0.182609444334755 2216 78-79 -0.1083022898903323 2216 80-81 -0.12973764343753302 2216 82-83 -0.2742579212617109 2216 84-85 -0.19733973397778826 2216 86-87 -0.13052421255349245 2216 88-89 -0.04449457650466471 2216 90-91 -0.04477497068661407 2216 92-93 -0.010575255146914486 2216 94-95 0.09260324316016622 2216 96-97 0.1457795064557068 2216 98-99 0.19948367370627906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 7.0 7 252.0 8 1356.0 9 2491.0 10 3755.0 11 6833.0 12 13603.0 13 25439.0 14 37251.0 15 41143.0 16 43249.0 17 45355.0 18 46390.0 19 48749.0 20 51776.0 21 56164.0 22 62840.0 23 69552.0 24 77801.0 25 87854.0 26 97154.0 27 110361.0 28 132661.0 29 165469.0 30 209760.0 31 270751.0 32 362265.0 33 486902.0 34 661851.0 35 938685.0 36 1403288.0 37 1675746.0 38 505747.0 39 387.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44957029593742 18.258964647165843 12.23274987740361 25.05871517949313 2 15.932834577553615 20.913090905615512 37.44802546490684 25.706049051924033 3 18.392165660715946 26.615530246523473 28.37313157747564 26.619172515284934 4 12.969671390012536 17.7008653232315 35.671578314393585 33.65788497236238 5 14.397908362610238 37.709066547531265 32.88109927980171 15.01192581005679 6 31.981972615640654 36.13316325034835 17.690688240704016 14.194175893306978 7 27.94446471410629 31.903342772179403 21.79266611346634 18.359526400247972 8 27.27332841096609 33.20934426655071 19.87159311507452 19.645734207408683 9 27.454991917097587 15.71191727323413 18.782709860030245 38.05038094963803 10-11 25.378886551976425 26.052459774360592 26.231824442698038 22.336829230964945 12-13 26.51356472010415 23.879564193168434 25.794975512987417 23.811895573740006 14-15 24.781427355071937 24.631201544943593 23.20136671067599 27.38600438930848 16-17 24.294854856220056 26.88353187718152 24.628464831017528 24.1931484355809 18-19 24.9915309367165 26.322126359328244 25.607541476454454 23.0788012275008 20-21 26.084630965168422 25.665181475591726 24.495126946835207 23.755060612404648 22-23 25.08528961920835 25.67850538865855 23.996031193809284 25.240173798323816 24-25 24.651421815668755 26.356203192871426 23.969563508653383 25.02281148280644 26-27 25.082949271854567 25.843331588890884 23.973786092781264 25.09993304647329 28-29 24.60884100596334 25.767483099463334 24.794828437394962 24.828847457178366 30-31 25.264304444416602 25.911044249401467 24.438214618424805 24.386436687757122 32-33 24.22448757661295 26.277739261787957 24.21014524948029 25.287627912118793 34-35 24.426990142951865 26.40529642306499 24.81711599525035 24.350597438732795 36-37 25.036473852640405 26.090152166336654 24.28599039207075 24.587383588952193 38-39 25.234134307218326 25.706486622004427 24.250964063436925 24.808415007340322 40-41 24.667569503322092 26.293114610164615 24.562095054609003 24.477220831904287 42-43 25.386231612980758 25.961613282326347 24.325060486366766 24.327094618326132 44-45 24.938931039408306 26.181529729354146 24.21014593266645 24.6693932985711 46-47 24.279280675516155 26.222668226821753 25.200547087495256 24.297504010166836 48-49 25.0539253447086 26.2261312182204 24.713231723000245 24.006711714070754 50-51 24.045626555353152 26.612212314758114 24.57535634163051 24.766804788258227 52-53 23.93958124781008 26.420950860411935 24.115937248518346 25.523530643259644 54-55 24.369282413652684 26.2530500574269 24.169989824208983 25.207677704711433 56-57 24.697551241046487 25.554607511287365 24.109214183089524 25.638627064576625 58-59 24.425960239378412 25.581937331643868 24.969491353806404 25.022611075171312 60-61 24.57615845287342 26.04079624222026 24.500165959134016 24.882879345772306 62-63 23.801848251891673 26.25156853699474 24.512319957266566 25.434263253847018 64-65 23.733479120095268 26.056471318475815 24.75767374283941 25.452375818589505 66-67 24.779200638117388 25.83156222006164 24.417967147699997 24.97126999412097 68-69 25.00220851022941 25.466673709790904 24.3167243892888 25.21439339069088 70-71 24.06141537479197 25.86352859310009 24.563431290829435 25.511624741278503 72-73 25.008976138968713 25.785586516418586 24.47076297181234 24.73467437280036 74-75 25.338794443523156 25.45749906880606 24.465021979276607 24.738684508394176 76-77 24.65961941536718 25.781844397114924 24.979713612911585 24.578822574606313 78-79 24.95345076513549 25.688881535894215 24.892188007119316 24.465479691850973 80-81 24.382126460065866 25.592172532028428 25.20120079241761 24.8245002154881 82-83 24.540890107444753 25.769434381055174 24.49201929570496 25.19765621579511 84-85 24.725192799151475 25.823388389592317 24.190866720372213 25.26055209088399 86-87 24.736593727895034 25.187110440847192 24.511496809910827 25.564799021346946 88-89 24.832428427360835 24.79626619817638 24.920244526410727 25.45106084805206 90-91 25.23104915216433 25.107922384481235 24.602190180260894 25.058838283093536 92-93 24.56591449675037 25.355342264455103 24.67832088986963 25.400422348924888 94-95 23.957510938749333 25.545981750734576 25.115941896091215 25.380565414424876 96-97 24.94194959344858 25.346547543407276 24.513309408198733 25.19819345494541 98-99 24.848645629514003 25.39703379079274 24.257500007910874 25.496820571782386 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 837.0 1 798.5 2 769.0 3 800.5 4 772.5 5 677.5 6 615.0 7 601.5 8 626.5 9 587.0 10 546.5 11 561.5 12 552.0 13 566.5 14 631.0 15 773.5 16 888.0 17 1068.5 18 1379.0 19 1763.5 20 2407.5 21 3443.5 22 5132.5 23 7236.0 24 9726.0 25 13409.0 26 18323.0 27 23885.0 28 31058.0 29 39825.0 30 48556.0 31 59029.0 32 69585.0 33 79087.0 34 88236.5 35 98747.0 36 107677.0 37 119209.0 38 132479.5 39 134539.0 40 137489.5 41 148663.0 42 165017.5 43 189626.5 44 216269.0 45 244536.5 46 284060.0 47 321417.0 48 352554.5 49 403104.5 50 456082.0 51 418251.5 52 354615.0 53 346505.5 54 331873.0 55 315384.0 56 289016.5 57 265056.5 58 251811.0 59 224327.0 60 191986.5 61 152511.0 62 123528.5 63 99558.5 64 74223.5 65 57349.0 66 41065.0 67 30485.0 68 24829.0 69 21760.0 70 19237.0 71 16856.0 72 16368.5 73 14407.5 74 10521.5 75 7872.5 76 5739.0 77 3903.5 78 2468.5 79 1630.0 80 1181.5 81 812.5 82 536.5 83 390.0 84 290.0 85 211.0 86 144.5 87 99.5 88 71.5 89 52.0 90 41.0 91 33.5 92 20.0 93 11.0 94 5.5 95 6.0 96 5.5 97 6.5 98 9.5 99 8.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.001976007140489071 3 0.005940936500816814 4 0.0 5 0.005630974596426372 6 0.0 7 8.007349196753096E-4 8 0.0 9 0.0 10-11 2.583015869920354E-5 12-13 9.040555544721238E-5 14-15 0.002705709123741571 16-17 1.67896031544823E-4 18-19 0.0 20-21 0.0 22-23 5.811785707320797E-5 24-25 9.815460305697345E-4 26-27 0.002621761107969159 28-29 0.002059955156261482 30-31 0.0026605063460179647 32-33 9.169706338217257E-4 34-35 1.291507934960177E-5 36-37 1.4852341252042034E-4 38-39 0.06208278643353571 40-41 0.003273972615124049 42-43 3.422496027644469E-4 44-45 1.291507934960177E-5 46-47 8.653103164233186E-4 48-49 8.39480157724115E-5 50-51 9.234281734965266E-4 52-53 3.35792063089646E-4 54-55 0.0 56-57 6.005511897564823E-4 58-59 0.0 60-61 2.195563489432301E-4 62-63 3.874523804880531E-5 64-65 9.234281734965266E-4 66-67 0.0014335738078057965 68-69 0.0014335738078057965 70-71 6.457539674800886E-5 72-73 0.0017693658708954425 74-75 0.0017951960295946462 76-77 1.2915079349601772E-4 78-79 1.2915079349601772E-4 80-81 0.0 82-83 3.0996190439044245E-4 84-85 6.392964278052876E-4 86-87 0.0 88-89 1.9372619024402653E-5 90-91 6.974142848784956E-4 92-93 0.0 94-95 0.0 96-97 0.0033902083292704647 98-99 0.00488835753382427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7742887.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.32311370727089 #Duplication Level Percentage of deduplicated Percentage of total 1 73.38997353554988 28.859222743120295 2 14.033842029001601 11.037087317126142 3 4.929459177124077 5.815250512122003 4 2.3812370088922563 3.745506146585273 5 1.2797951997577364 2.5162766081046466 6 0.8113110867732608 1.9141966870292675 7 0.5505580385437745 1.5154759446476211 8 0.37322021862422405 1.1740944875850292 9 0.27983652645734036 0.9903639194396743 >10 1.6773528840004492 12.172679953730391 >50 0.14247208647213266 3.9115622105518693 >100 0.12132035308228878 9.973692687852067 >500 0.017813070582390525 4.8304502614568126 >1k 0.011211711733290757 8.335699275775632 >5k 3.3170744738833324E-4 0.8435666721758467 >10k+ 2.653659579106666E-4 2.3648745726975746 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 50562 0.6530122420745647 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 44621 0.5762837556585806 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 20492 0.2646558060320395 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 14676 0.18954170453475558 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 13835 0.17868012280174048 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 13171 0.17010451011360492 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 12808 0.16541633630969946 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 11138 0.14384815379586452 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 8942 0.11548663954413901 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 8047 0.10392764352624544 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.291507934960177E-5 2 0.0 0.0 0.0 0.0 2.583015869920354E-5 3 0.0 0.0 0.0 0.0 2.583015869920354E-5 4 0.0 0.0 0.0 1.291507934960177E-5 2.583015869920354E-5 5 0.0 0.0 0.0 3.874523804880531E-5 2.583015869920354E-5 6 1.291507934960177E-5 0.0 0.0 3.874523804880531E-5 2.583015869920354E-5 7 1.291507934960177E-5 1.291507934960177E-5 0.0 3.874523804880531E-5 2.583015869920354E-5 8 1.291507934960177E-5 1.291507934960177E-5 0.0 3.874523804880531E-5 2.583015869920354E-5 9 1.291507934960177E-5 1.291507934960177E-5 0.0 6.457539674800885E-5 2.583015869920354E-5 10-11 1.291507934960177E-5 1.291507934960177E-5 0.0 7.103293642280973E-5 5.166031739840708E-5 12-13 1.291507934960177E-5 1.291507934960177E-5 0.0 9.04055554472124E-5 7.103293642280973E-5 14-15 1.291507934960177E-5 1.291507934960177E-5 0.0 1.0332063479681416E-4 9.04055554472124E-5 16-17 1.291507934960177E-5 1.291507934960177E-5 0.0 1.291507934960177E-4 1.0332063479681416E-4 18-19 2.583015869920354E-5 1.291507934960177E-5 0.0 2.2601388861803097E-4 1.1623571414641593E-4 20-21 7.103293642280973E-5 1.291507934960177E-5 0.0 4.261976185368584E-4 1.3560833317081858E-4 22-23 7.749047609761061E-5 1.291507934960177E-5 0.0 0.0015562670616270135 1.4852341252042034E-4 24-25 9.686309512201328E-5 1.291507934960177E-5 0.0 0.004320094042441792 1.5498095219522123E-4 26-27 1.291507934960177E-4 1.291507934960177E-5 0.0 0.007264732134150995 1.743535712196239E-4 28-29 1.6789603154482302E-4 1.291507934960177E-5 0.0 0.01875269521562177 1.808111108944248E-4 30-31 2.3247142829283185E-4 1.291507934960177E-5 0.0 0.05123411977987022 1.808111108944248E-4 32-33 2.7767420601643807E-4 1.291507934960177E-5 0.0 0.10822190740998802 1.8726865056922567E-4 34-35 2.841317456912389E-4 1.291507934960177E-5 0.0 0.17934524938824498 1.9372619024402655E-4 36-37 2.841317456912389E-4 3.2287698374004423E-5 0.0 0.2921649250466912 1.9372619024402655E-4 38-39 2.841317456912389E-4 5.166031739840708E-5 0.0 0.46688011848810396 1.9372619024402655E-4 40-41 3.0996190439044245E-4 5.166031739840708E-5 0.0 0.6887934694126364 1.9372619024402655E-4 42-43 3.2933452341484513E-4 5.166031739840708E-5 0.0 0.8822419854506465 1.9372619024402655E-4 44-45 3.3579206308964604E-4 5.166031739840708E-5 0.0 1.0994413324125745 1.9372619024402655E-4 46-47 3.4224960276444695E-4 5.166031739840708E-5 0.0 1.3415409523605342 1.9372619024402655E-4 48-49 3.487071424392478E-4 5.166031739840708E-5 0.0 1.5959098976906159 2.195563489432301E-4 50-51 3.487071424392478E-4 5.166031739840708E-5 0.0 1.8803645203655948 2.3247142829283185E-4 52-53 3.487071424392478E-4 5.166031739840708E-5 0.0 2.1715931021594397 2.3247142829283185E-4 54-55 3.487071424392478E-4 6.457539674800885E-5 0.0 2.4446178796100213 2.3247142829283185E-4 56-57 3.487071424392478E-4 6.457539674800885E-5 0.0 2.7481803621827363 2.3892896796763274E-4 58-59 3.487071424392478E-4 7.749047609761061E-5 0.0 3.0845342312240898 2.5184404731723453E-4 60-61 3.487071424392478E-4 7.749047609761061E-5 0.0 3.4213142978839803 2.6475912666683624E-4 62-63 3.487071424392478E-4 7.749047609761061E-5 0.0 3.76818104151591 2.841317456912389E-4 64-65 3.487071424392478E-4 7.749047609761061E-5 0.0 4.132689783539396 3.035043647156416E-4 66-67 3.487071424392478E-4 7.749047609761061E-5 0.0 4.524746389815582 3.0996190439044245E-4 68-69 3.487071424392478E-4 7.749047609761061E-5 0.0 4.952351752001547 3.0996190439044245E-4 70-71 3.487071424392478E-4 7.749047609761061E-5 0.0 5.447716077995198 3.228769837400443E-4 72-73 3.487071424392478E-4 7.749047609761061E-5 0.0 5.913059302040699 3.3579206308964604E-4 74-75 3.487071424392478E-4 7.749047609761061E-5 0.0 6.368567693161479 3.487071424392478E-4 76-77 3.487071424392478E-4 7.749047609761061E-5 0.0 6.862659883839193 3.616222217888496E-4 78-79 3.487071424392478E-4 9.04055554472124E-5 0.0 7.36984150743773 3.616222217888496E-4 80-81 3.487071424392478E-4 1.1623571414641593E-4 0.0 7.924331066693857 3.7453730113845134E-4 82-83 3.616222217888496E-4 1.291507934960177E-4 0.0 8.525476350100421 4.1328253918725664E-4 84-85 3.7453730113845134E-4 1.291507934960177E-4 0.0 9.107023000593966 4.1328253918725664E-4 86-87 3.7453730113845134E-4 1.291507934960177E-4 0.0 9.694510587588326 4.261976185368584E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATA 2255 0.0 21.85874 1 GTACTAG 2295 0.0 21.477758 1 GTATAAG 2930 0.0 21.26684 1 ATACGGG 1675 0.0 21.099133 3 TACGGGC 1705 0.0 20.452906 4 CGGGCGT 1880 0.0 17.806164 6 GTACAAG 4270 0.0 17.424404 1 TGATCGC 2085 0.0 17.394962 9 TAGTACT 3040 0.0 16.977257 4 GTGATCG 2140 0.0 16.946144 8 GGCGTCG 2025 0.0 16.760536 8 CATGGGG 18575 0.0 16.470818 4 ATAAGGT 2860 0.0 16.259224 3 TACATGA 2875 0.0 16.174393 2 GTATTAT 1840 0.0 16.17439 1 GTACAAA 4245 0.0 15.993406 1 CCGTCGT 3305 0.0 15.89798 9 CGTTAAC 650 0.0 15.738928 1 TAAGGTG 2940 0.0 15.024304 4 CTAGTAC 3545 0.0 14.822723 3 >>END_MODULE