FastQCFastQC Report
Fri 27 May 2016
ERR765410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742887
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT492080.6355252246352039No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA471870.6094238492696588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198450.2562997496928471No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG195770.2528385084271539No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC147940.1910656838980086No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC138900.17939045216596858No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG131230.16948458630482402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130020.16792186170352222No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG129200.16686282519685486No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT115460.14911750617050204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100850.13024857524073383No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC95730.12363605461373775No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG79960.10326897447941576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6550.034.789382
AGTACTT333000.021.77875312-13
TAACGCA7100.021.6133584
ATCACGC11850.020.4057063
TAGTACT30800.019.3252834
TACTTTT384050.019.08928714-15
GAGTACT315650.018.75488912-13
CGTTAAC7500.018.6104411
CTAGTAC32750.018.174623
GTACTAG22250.017.9831241
GTATAAC11550.017.7242321
CATGGGG153950.017.3984054
GTATCAA814950.017.150061
GTACTTT366050.016.34379414-15
TCTAACG11500.016.1753642
ATTTAGG31800.016.0939351
TCACGCA13650.016.0114944
CTAACGC11200.015.7772823
GGCTATT30400.015.7550247
TAGGCAT35550.015.6966975
Produced by FastQC (version 0.11.5)