FastQCFastQC Report
Fri 27 May 2016
ERR765409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21093212
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA619460.2936774162228114No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT605390.28700702387099697No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG254440.12062648400822026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG72000.019.5128231
GTGATCG48300.019.259548
AATACGG36750.018.7943345
CATGGGG427750.018.464894
GTATAAG82100.018.1997471
TGATCGC50600.018.0127099
TAATACG39800.017.7083154
TAGTACT88400.017.3274384
GTACATG1663900.016.9746511
TACATGG1632050.016.8537882
ACATGGG1502550.016.6489323
GTATAAT69450.015.8316061
TATACCG40600.015.7390995
GTATAAA172700.015.725951
CTAGTAC95150.015.703473
ACGCCTA35950.015.6823166
CTGTGCG93550.015.6186859
GTATTAG80150.015.4768231
TAGGCAT115450.014.8547695
TTAATAC92250.014.82193
Produced by FastQC (version 0.11.5)