##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765409_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21093212
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.947585697237574	33.0	33.0	33.0	27.0	33.0
2	31.826558183741763	33.0	33.0	33.0	27.0	33.0
3	31.884884388399453	33.0	33.0	33.0	27.0	33.0
4	35.66569984694602	37.0	37.0	37.0	33.0	37.0
5	35.700836079398435	37.0	37.0	37.0	33.0	37.0
6	35.70180781381233	37.0	37.0	37.0	33.0	37.0
7	35.647353091601225	37.0	37.0	37.0	33.0	37.0
8	35.64273781536923	37.0	37.0	37.0	33.0	37.0
9	35.64185582546651	37.0	37.0	37.0	33.0	37.0
10-11	35.60688746692538	37.0	37.0	37.0	33.0	37.0
12-13	35.56052304883676	37.0	37.0	37.0	33.0	37.0
14-15	37.12781678295369	40.0	37.0	40.0	33.0	40.0
16-17	37.150125831950106	40.0	37.0	40.0	33.0	40.0
18-19	37.11421740795095	40.0	37.0	40.0	33.0	40.0
20-21	37.20664169117534	40.0	37.0	40.0	33.0	40.0
22-23	37.18515532390231	40.0	37.0	40.0	33.0	40.0
24-25	37.15937053588614	40.0	37.0	40.0	33.0	40.0
26-27	37.10812338585512	40.0	37.0	40.0	33.0	40.0
28-29	37.059985458829125	40.0	37.0	40.0	33.0	40.0
30-31	36.99028331958168	37.0	37.0	40.0	33.0	40.0
32-33	36.88852527533503	37.0	37.0	40.0	33.0	40.0
34-35	36.79242973995615	37.0	37.0	40.0	33.0	40.0
36-37	36.727048848700704	37.0	37.0	40.0	33.0	40.0
38-39	36.6396050776904	37.0	37.0	40.0	33.0	40.0
40-41	36.55961088334958	37.0	37.0	40.0	33.0	40.0
42-43	36.4370398638197	37.0	37.0	40.0	33.0	40.0
44-45	36.30873427432484	37.0	37.0	40.0	33.0	40.0
46-47	36.15810181019373	37.0	37.0	40.0	33.0	40.0
48-49	35.943202415070786	37.0	37.0	40.0	27.0	40.0
50-51	35.86445281543655	37.0	37.0	40.0	27.0	40.0
52-53	35.72783542876258	37.0	37.0	40.0	27.0	40.0
54-55	35.57625128406238	37.0	33.0	40.0	27.0	40.0
56-57	35.423115455341744	37.0	33.0	40.0	27.0	40.0
58-59	35.261204149467616	37.0	33.0	40.0	27.0	40.0
60-61	35.07491703966186	37.0	33.0	40.0	27.0	40.0
62-63	34.85531665352816	37.0	33.0	38.5	27.0	40.0
64-65	34.6579741387893	37.0	33.0	37.0	27.0	40.0
66-67	34.45607508709437	37.0	33.0	37.0	27.0	40.0
68-69	34.24385010684954	37.0	33.0	37.0	27.0	40.0
70-71	34.01675697850095	37.0	33.0	37.0	27.0	40.0
72-73	33.77205624254855	37.0	33.0	37.0	27.0	38.5
74-75	33.53230958850648	37.0	33.0	37.0	27.0	37.0
76-77	33.30888619523665	37.0	33.0	37.0	27.0	37.0
78-79	33.10838043537419	37.0	33.0	37.0	27.0	37.0
80-81	32.88296360459469	37.0	33.0	37.0	27.0	37.0
82-83	32.69092481031338	37.0	33.0	37.0	27.0	37.0
84-85	32.49645179690983	33.0	33.0	37.0	22.0	37.0
86-87	32.328266861395974	33.0	33.0	37.0	22.0	37.0
88-89	32.1425821017681	33.0	33.0	37.0	22.0	37.0
90-91	31.95253356861914	33.0	33.0	37.0	22.0	37.0
92-93	31.78532645952641	33.0	33.0	37.0	22.0	37.0
94-95	31.618404489558063	33.0	33.0	37.0	22.0	37.0
96-97	31.483166693626366	33.0	33.0	37.0	18.5	37.0
98-99	31.315027270384427	33.0	33.0	37.0	15.0	37.0
100	31.19963773179732	33.0	33.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	17.0
7	243.0
8	1379.0
9	2751.0
10	4267.0
11	6818.0
12	13229.0
13	25441.0
14	37845.0
15	42563.0
16	47166.0
17	54268.0
18	64942.0
19	76457.0
20	90217.0
21	109666.0
22	127931.0
23	135432.0
24	142686.0
25	164772.0
26	197733.0
27	245989.0
28	312189.0
29	400688.0
30	523286.0
31	684364.0
32	893600.0
33	1164482.0
34	1509817.0
35	2041399.0
36	3279122.0
37	5693884.0
38	2994712.0
39	3852.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.66152988196899	19.362428345120502	12.726083938529095	25.249957834381416
2	15.843404759858165	21.44588373629707	37.76042127200208	24.95029023184269
3	18.170526027626654	26.3155953355882	29.60896273005954	25.904915906725606
4	12.921295497121207	17.649189509505128	37.39595932193618	32.03355567143748
5	13.834823233044272	37.60171094753524	34.11276351266169	14.450702306758803
6	30.880474723337535	37.548245378655466	17.93376940410972	13.63751049389728
7	26.70288906213051	32.024567903645966	23.02006446434047	18.252478569883053
8	26.054339187412516	33.58120138364892	20.92879453352102	19.43566489541754
9	26.722539933699995	14.83301357801742	20.212445596242052	38.23200089204053
10-11	24.917558786210463	25.75712508839337	27.93690927678535	21.38840684861082
12-13	25.909695498248443	23.665091878847093	27.400331443120184	23.024881179784284
14-15	23.780569787095487	24.503096067113912	24.99200453681497	26.724329608975626
16-17	23.427691006356977	26.953330033838274	25.965285421295892	23.65369353850886
18-19	23.719550077859324	26.517057356333005	27.36171800780165	22.40167455800602
20-21	24.797147233597272	25.86941412201766	26.27718075835288	23.056257886032185
22-23	24.11879964728138	25.71295543032634	25.970899528720782	24.197345393671498
24-25	23.736075734076934	26.217741279169758	26.155839980496538	23.890343006256767
26-27	24.00480419002366	25.96638868613961	26.077359644092628	23.9514474797441
28-29	23.581069764565836	25.95419414764274	26.628503895577726	23.8362321922137
30-31	24.151950247229397	25.890844745173247	26.33490028600806	23.622304721589295
32-33	23.53693203168314	26.018018384521646	26.072021476630734	24.373028107164476
34-35	23.895510259354065	25.975498212131587	26.568805427186483	23.560186101327865
36-37	24.114783612244064	25.73767755725596	26.212813614548438	23.934725215951538
38-39	24.046422411500863	25.351502679422488	26.419245836393706	24.18282907268294
40-41	23.778771136392354	25.52782309767993	26.7982752357177	23.895130530210015
42-43	24.187681792420364	25.322221786249038	26.566316440584277	23.923779980746318
44-45	23.7250826785045	25.54753164362944	26.42177949523775	24.305606182628306
46-47	23.3401061868299	25.410834958147216	26.986398838446263	24.262660016576625
48-49	23.7667478171977	25.622349657558036	26.65887877608973	23.952023749154534
50-51	22.967264724586926	25.890623253854788	26.432294833731063	24.709817187827227
52-53	22.881712398009167	25.74783636548667	26.276542228893284	25.093909007610883
54-55	23.078448931720075	25.669174190585963	26.38409698611778	24.86827989157618
56-57	23.19506384426996	25.149889733113667	26.18399903406383	25.47104738855254
58-59	22.903737137446267	25.16269828004574	26.652472604654236	25.281091977853755
60-61	23.053859925317166	25.274877972352748	26.356504256567774	25.31475784576231
62-63	22.61453091039586	25.19621467073756	26.267392331470045	25.92186208739653
64-65	22.47946606883491	25.034958824745296	26.431877545575556	26.053697560844242
66-67	23.08956289961592	25.070844210163155	26.11578327067628	25.723809619544635
68-69	23.129847773128976	24.82994812360772	26.00459941867319	26.03560468459011
70-71	22.56209781815408	24.85084532951667	26.201566603501785	26.38549024882747
72-73	23.01906024483863	24.76886638839465	26.232130790804142	25.97994257596258
74-75	23.083418648107635	24.661469363628115	26.257571538062052	25.9975404502022
76-77	22.793554906725614	24.899946149285103	26.280817776065042	26.02568116792424
78-79	22.922666771098076	24.959197788506863	26.167912336785225	25.950223103609833
80-81	22.612137477117088	24.80923853144853	26.50156786448849	26.07705612694589
82-83	22.721581850390866	24.801148314806447	26.178411633281968	26.298858201520726
84-85	22.772810189282076	24.81325802535179	25.909404040222782	26.504527745143353
86-87	22.71628186583719	24.50455678330875	26.188153453820345	26.59100789703372
88-89	22.871130596283002	24.222612162705925	26.32201142916743	26.584245811843644
90-91	23.126823601992154	24.279810476834268	26.118390749859376	26.474975171314195
92-93	23.014950926667595	24.24762682226856	26.137621137200025	26.59980111386382
94-95	22.580024623416918	24.22136213336446	26.532339764846057	26.66627347837256
96-97	23.04750981212105	24.05321236634109	26.22027399577484	26.679003825763026
98-99	23.028421302904	24.014428142677087	26.15165218661055	26.80549836780836
100	23.04950536356898	24.080275867104195	26.085105485222066	26.78511328410476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1161.0
1	1049.0
2	1201.5
3	1463.0
4	1223.0
5	1043.0
6	1366.0
7	2585.0
8	3966.5
9	3764.0
10	2418.5
11	1666.0
12	1747.5
13	2038.0
14	2415.5
15	2900.0
16	3500.0
17	4244.5
18	5218.5
19	6450.0
20	8064.5
21	10435.0
22	13408.0
23	17517.0
24	23274.5
25	31102.0
26	43104.0
27	58607.5
28	76965.0
29	102593.5
30	131312.5
31	159963.5
32	193126.5
33	227826.0
34	266088.5
35	301484.5
36	336931.0
37	383474.5
38	419266.0
39	447635.5
40	476016.5
41	504187.0
42	538709.5
43	593436.0
44	665571.5
45	734255.0
46	811598.5
47	892111.5
48	976293.5
49	1046800.5
50	1119552.5
51	1116555.0
52	1006630.5
53	924649.5
54	863725.5
55	797783.0
56	741281.5
57	666754.5
58	600420.0
59	539278.5
60	453552.0
61	364205.0
62	291984.0
63	226949.5
64	168922.5
65	135434.5
66	107471.5
67	79349.0
68	61720.5
69	49394.5
70	41394.0
71	36699.5
72	32232.5
73	32769.5
74	26415.5
75	19130.5
76	14557.0
77	10158.5
78	7269.0
79	4455.5
80	2958.0
81	2215.0
82	1665.5
83	1215.5
84	840.0
85	572.0
86	382.5
87	217.0
88	123.0
89	84.0
90	61.0
91	42.5
92	26.5
93	22.5
94	22.0
95	10.5
96	8.0
97	11.5
98	9.5
99	6.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017801935523143655
2	0.006073043782995212
3	0.0063290503124891545
4	0.004081881886931208
5	0.04593894945919095
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.053436716987437E-4
18-19	0.0017185623507695272
20-21	6.755727861645727E-4
22-23	0.004954200431873533
24-25	0.007445523232782186
26-27	0.005722220020355363
28-29	0.022206196002770937
30-31	0.020366741679740383
32-33	0.013051592142533815
34-35	0.013622865972237892
36-37	0.01342612021346014
38-39	0.021013869295961184
40-41	0.030286994697630687
42-43	0.01772845216745558
44-45	0.016785020697653825
46-47	0.01836372762953314
48-49	0.013165372822308903
50-51	0.010007958958550266
52-53	0.027392698655851942
54-55	0.025650431996795935
56-57	0.013234115316339683
58-59	0.01615211566640491
60-61	0.017192734800181214
62-63	0.02048052235951547
64-65	0.011039096369011982
66-67	0.015521581065984641
68-69	0.007744197517191787
70-71	0.008775334927653504
72-73	0.010517601586709507
74-75	0.0034797924564547115
76-77	0.007443152801953538
78-79	0.005774369498585612
80-81	0.0024130985835632813
82-83	1.9674575877775278E-4
84-85	0.002552954002453491
86-87	0.0022850953188163093
88-89	0.0011828449834951642
90-91	8.083169125688396E-4
92-93	0.0011306955052649164
94-95	0.0032640832510477777
96-97	0.0014009246197307455
98-99	0.0019295306945191655
100	0.0021191651608109758
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.1093212E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.64240907020961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88508047673132	29.215828467802524
2	15.286806343383743	12.425852735697546
3	5.464486468915556	6.662696832848739
4	2.4938878780921865	4.054304452666387
5	1.3127287729433672	2.6676229894099315
6	0.794086226827238	1.9364146360639114
7	0.5196607471464791	1.4784185264280578
8	0.37238106491201356	1.2107570856123469
9	0.2571613799876956	0.9406492202267586
>10	1.3924704257641776	10.228837703853838
>50	0.10771058697726651	3.0597860365357112
>100	0.08464483925628881	7.235815054056087
>500	0.01434720755047279	4.066740504226166
>1k	0.013326883866948016	10.33582671268065
>5k	9.85948098127066E-4	2.643642393356506
>10k+	2.347495471731098E-4	1.836806648534972
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	61946	0.2936774162228114	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	60539	0.28700702387099697	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	25444	0.12062648400822026	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.740861657295248E-6
2	4.740861657295248E-6	4.740861657295248E-6	0.0	0.0	4.740861657295248E-6
3	4.740861657295248E-6	4.740861657295248E-6	0.0	0.0	4.740861657295248E-6
4	4.740861657295248E-6	1.4222584971885742E-5	0.0	0.0	1.4222584971885742E-5
5	4.740861657295248E-6	1.4222584971885742E-5	0.0	0.0	1.896344662918099E-5
6	4.740861657295248E-6	1.4222584971885742E-5	0.0	0.0	2.3704308286476237E-5
7	4.740861657295248E-6	1.4222584971885742E-5	0.0	0.0	2.8445169943771484E-5
8	9.481723314590495E-6	1.4222584971885742E-5	0.0	0.0	2.8445169943771484E-5
9	9.481723314590495E-6	3.792689325836198E-5	0.0	4.740861657295248E-6	3.792689325836198E-5
10-11	9.481723314590495E-6	3.792689325836198E-5	0.0	7.111292485942872E-6	3.792689325836198E-5
12-13	1.6593015800533366E-5	3.792689325836198E-5	0.0	3.318603160106673E-5	5.689033988754297E-5
14-15	3.0815600772419106E-5	4.7408616572952475E-5	0.0	4.503818574430485E-5	9.00763714886097E-5
16-17	4.7408616572952475E-5	5.4519909058895343E-5	0.0	6.637206320213346E-5	1.208919722610288E-4
18-19	4.7408616572952475E-5	6.400163237348584E-5	0.0	9.95580948032002E-5	1.4696671137615268E-4
20-21	5.214947823024772E-5	7.585378651672396E-5	0.0	1.9200489712045752E-4	1.493371422048003E-4
22-23	5.4519909058895343E-5	8.05946481740192E-5	0.0	8.746889757709731E-4	1.5407800386209555E-4
24-25	6.637206320213346E-5	8.296507900266682E-5	0.0	0.0029085186267506346	1.5644843469074318E-4
26-27	1.2326240308967642E-4	8.533550983131445E-5	0.0	0.005700886142897535	1.6593015800533365E-4
28-29	1.3274412640426692E-4	9.00763714886097E-5	0.0	0.02428032297783761	1.7304145049127652E-4
30-31	1.493371422048003E-4	1.1141024894643831E-4	0.0	0.09596215123614175	1.7541188131992415E-4
32-33	1.5170757303344793E-4	1.1378067977508594E-4	0.0	0.2561416440511763	1.8489360463451465E-4
34-35	1.5407800386209555E-4	1.303736955756193E-4	0.0	0.4764637078506583	2.0385705126369565E-4
36-37	1.5644843469074318E-4	1.4459628054750505E-4	0.0	0.8303903644452064	2.085979129209909E-4
38-39	1.5644843469074318E-4	1.5407800386209552E-4	0.0	1.399779701640509	2.1570920540693375E-4
40-41	1.5644843469074318E-4	1.7541188131992415E-4	0.0	2.07489973551681	2.2282049789287662E-4
42-43	1.611892963480384E-4	1.7778231214857177E-4	0.0	2.658082609704013	2.3230222120746712E-4
44-45	1.6593015800533365E-4	1.9200489712045752E-4	0.0	3.333107826347168	2.441543753507052E-4
46-47	1.6593015800533365E-4	2.0385705126369565E-4	0.0	4.108226855160797	2.631178219798862E-4
48-49	1.6593015800533365E-4	2.0385705126369565E-4	0.0	4.902385184390125	3.0341514606689585E-4
50-51	1.6593015800533365E-4	2.1807963623558137E-4	0.0	5.711892053234946	3.081560077241911E-4
52-53	1.6593015800533365E-4	2.1807963623558137E-4	0.0	6.494634387593506	3.4608290098255305E-4
54-55	1.6593015800533365E-4	2.1807963623558137E-4	0.0	7.268184191198571	3.7452807092632455E-4
56-57	1.706710196626289E-4	2.1807963623558137E-4	0.0	8.143664890866312	3.816393634122674E-4
58-59	1.706710196626289E-4	2.1807963623558137E-4	0.0	9.08924634142965	4.006028100414484E-4
60-61	1.706710196626289E-4	2.2282049789287662E-4	0.0	10.020974994230372	4.2904797998521987E-4
62-63	1.706710196626289E-4	2.2282049789287662E-4	0.0	10.92967254109995	4.503818574430485E-4
64-65	1.706710196626289E-4	2.3704308286476237E-4	0.0	11.83036514306119	4.503818574430485E-4
66-67	1.706710196626289E-4	2.3704308286476237E-4	0.0	12.782754470964402	4.574931499289914E-4
68-69	1.706710196626289E-4	2.3704308286476237E-4	0.0	13.821508075678564	4.669748732435819E-4
70-71	1.706710196626289E-4	2.417839445220576E-4	0.0	14.946486101784782	4.764565965581724E-4
72-73	1.7541188131992415E-4	2.488952370080005E-4	0.0	15.998087441590213	5.404582289316582E-4
74-75	1.801527429772194E-4	2.607473911512386E-4	0.0	16.99485360503654	5.451990905889534E-4
76-77	1.801527429772194E-4	2.77340406951772E-4	0.0	18.028655379749654	5.499399522462487E-4
78-79	1.801527429772194E-4	2.915629919236577E-4	0.0	19.09380136130998	5.54680813903544E-4
80-81	1.801527429772194E-4	2.986742844096006E-4	0.0	20.214858694825615	5.73644260532725E-4
82-83	1.801527429772194E-4	3.1763773103878155E-4	0.0	21.35613817374044	6.163120154483822E-4
84-85	1.801527429772194E-4	3.247490235247244E-4	0.0	22.426058202989665	6.234233079343251E-4
86-87	1.801527429772194E-4	3.318603160106673E-4	0.0	23.491683011577372	6.329050312489156E-4
88	1.801527429772194E-4	3.7452807092632455E-4	0.0	24.304297515238552	6.447571853921536E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	7200	0.0	19.512823	1
GTGATCG	4830	0.0	19.25954	8
AATACGG	3675	0.0	18.794334	5
CATGGGG	42775	0.0	18.46489	4
GTATAAG	8210	0.0	18.199747	1
TGATCGC	5060	0.0	18.012709	9
TAATACG	3980	0.0	17.708315	4
TAGTACT	8840	0.0	17.327438	4
GTACATG	166390	0.0	16.974651	1
TACATGG	163205	0.0	16.853788	2
ACATGGG	150255	0.0	16.648932	3
GTATAAT	6945	0.0	15.831606	1
TATACCG	4060	0.0	15.739099	5
GTATAAA	17270	0.0	15.72595	1
CTAGTAC	9515	0.0	15.70347	3
ACGCCTA	3595	0.0	15.682316	6
CTGTGCG	9355	0.0	15.618685	9
GTATTAG	8015	0.0	15.476823	1
TAGGCAT	11545	0.0	14.854769	5
TTAATAC	9225	0.0	14.8219	3
>>END_MODULE