FastQCFastQC Report
Fri 27 May 2016
ERR765408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9763390
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1009461.0339236679063317No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT830520.8506471625122012No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG427620.43798311856844807No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG386860.3962353240011922No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT364300.37312859570292695No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG312880.32046246232097664No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC291220.29827754499205705No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA280310.28710314757476657No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC278830.2855872806473981No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC277950.2846859543662601No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC231000.23659814879872665No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT205150.21012168929029773No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG156530.1603234122574229No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA130910.13408252666338227No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT127210.13029285934496113No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC120650.12357388161284143No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC118500.12137177763051563No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG109900.1125633617012124No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA107370.1099720486429406No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG104780.1073192815200458No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG43350.024.3885461
GTACATA33500.022.8631081
AATACGG35350.020.0696585
TAATACG38650.019.5721134
GTACAAA48450.018.4269031
GTACTAG28350.017.7346741
GTATAAG29450.017.7104721
GGTCGCC73300.017.49882594
TCTAACG12950.017.4159092
AGTACTC34950.017.3418335
CTAACGC13050.017.2824543
GTGATCG18500.017.0147048
TGATCGC19050.016.7699139
TACATGA41650.016.4707182
TTAATAC58500.016.3047773
ATGGGTA58300.016.1181015
GTATAGA23550.015.9621561
CGTTAAC9400.015.4962391
GGGTACC50200.015.4423247
CATGGGT82400.015.3386254
Produced by FastQC (version 0.11.5)