FastQCFastQC Report
Fri 27 May 2016
ERR765407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14703864
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA615160.41836621992695255No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT588210.4000377043748501No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG235460.16013477817803537No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG196720.13378796213022645No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG183370.12470871602185657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182360.12402182174699114No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC175910.11963521969463266No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC170850.11619394738689094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG55150.020.6237221
TAGTACT57600.019.3374614
CATGGGG351850.018.6199634
GTACTAG51850.017.5853181
TAATACG25800.017.1230744
ATTTAGG74900.016.7543141
CTATTGA69850.016.6189739
TCTAACG27300.016.3546752
GTATATA33200.016.2800961
GGGCTAT81700.016.2793856
TTTAGGG79150.016.2103612
CTAGTAC70900.016.1739353
GTGATCG42900.015.9944458
CTAACGC28300.015.776453
GTACATA37150.015.687731
GTACATG1322500.015.4628891
GTCCTAC52150.015.4112921
TAGGCAT74600.015.3717965
CCTACAC65700.015.3080723
AATACGG24050.015.2423935
Produced by FastQC (version 0.11.5)