##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765407_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14703864
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.171767298718215	33.0	33.0	33.0	33.0	33.0
2	32.13572187555597	33.0	33.0	33.0	33.0	33.0
3	32.11886950260149	33.0	33.0	33.0	33.0	33.0
4	35.958163582035304	37.0	37.0	37.0	33.0	37.0
5	35.92679971740761	37.0	37.0	37.0	33.0	37.0
6	35.962238429299944	37.0	37.0	37.0	33.0	37.0
7	35.91841097006882	37.0	37.0	37.0	33.0	37.0
8	35.91226340232744	37.0	37.0	37.0	33.0	37.0
9	35.931704346558156	37.0	37.0	37.0	33.0	37.0
10-11	35.904870175621866	37.0	37.0	37.0	33.0	37.0
12-13	35.881593402931365	37.0	37.0	37.0	33.0	37.0
14-15	37.75552232392791	40.0	37.0	40.0	33.0	40.0
16-17	37.786560933915055	40.0	37.0	40.0	33.0	40.0
18-19	37.825138208568845	40.0	37.0	40.0	33.0	40.0
20-21	37.843209172772546	40.0	37.0	40.0	33.0	40.0
22-23	37.835440636556484	40.0	37.0	40.0	33.0	40.0
24-25	37.83936300689397	40.0	37.0	40.0	33.0	40.0
26-27	37.81930392582521	40.0	37.0	40.0	33.0	40.0
28-29	37.78236989270303	40.0	37.0	40.0	33.0	40.0
30-31	37.734170181389054	40.0	37.0	40.0	33.0	40.0
32-33	37.66125424582273	40.0	37.0	40.0	33.0	40.0
34-35	37.5710037851275	40.0	37.0	40.0	33.0	40.0
36-37	37.49940093978019	40.0	37.0	40.0	33.0	40.0
38-39	37.38128137610631	40.0	37.0	40.0	33.0	40.0
40-41	37.297089118887385	40.0	37.0	40.0	33.0	40.0
42-43	37.16045901267857	40.0	37.0	40.0	33.0	40.0
44-45	36.986868349707265	40.0	37.0	40.0	33.0	40.0
46-47	36.79849864634221	37.0	37.0	40.0	33.0	40.0
48-49	36.616387195909866	37.0	37.0	40.0	33.0	40.0
50-51	36.43545009665486	37.0	37.0	40.0	33.0	40.0
52-53	36.267715989484124	37.0	37.0	40.0	33.0	40.0
54-55	36.12420432479517	37.0	37.0	40.0	33.0	40.0
56-57	35.94906825171941	37.0	37.0	40.0	33.0	40.0
58-59	35.75658044715321	37.0	37.0	40.0	33.0	40.0
60-61	35.5577607695501	37.0	35.0	40.0	27.0	40.0
62-63	35.33411639280668	37.0	33.0	38.5	27.0	40.0
64-65	35.12003939916745	37.0	33.0	37.0	27.0	40.0
66-67	34.91000001768243	37.0	33.0	37.0	27.0	40.0
68-69	34.69903815758906	37.0	33.0	37.0	27.0	40.0
70-71	34.47761690396484	37.0	33.0	37.0	27.0	40.0
72-73	34.27228594470134	37.0	33.0	37.0	27.0	37.0
74-75	34.06332468118585	37.0	33.0	37.0	27.0	37.0
76-77	33.87587711638247	37.0	33.0	37.0	27.0	37.0
78-79	33.70527417146948	37.0	33.0	37.0	27.0	37.0
80-81	33.55492444026958	37.0	33.0	37.0	27.0	37.0
82-83	33.40589684453012	37.0	33.0	37.0	27.0	37.0
84-85	33.27086210128168	37.0	33.0	37.0	27.0	37.0
86-87	33.15732626471518	37.0	33.0	37.0	27.0	37.0
88-89	33.058609117984226	37.0	33.0	37.0	27.0	37.0
90-91	32.931275581711034	37.0	33.0	37.0	27.0	37.0
92-93	32.813799488352174	37.0	33.0	37.0	27.0	37.0
94-95	32.729763856629795	37.0	33.0	37.0	27.0	37.0
96-97	32.665634659025685	37.0	33.0	37.0	24.5	37.0
98-99	32.53932313302136	35.0	33.0	37.0	22.0	37.0
100	32.43795358825408	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	186.0
8	1490.0
9	3107.0
10	3505.0
11	4587.0
12	7374.0
13	13659.0
14	21476.0
15	24454.0
16	27381.0
17	32226.0
18	38245.0
19	46245.0
20	55111.0
21	68269.0
22	84911.0
23	90810.0
24	88224.0
25	90615.0
26	99935.0
27	115098.0
28	140376.0
29	179177.0
30	237099.0
31	312026.0
32	424086.0
33	581563.0
34	813021.0
35	1230347.0
36	2349569.0
37	4908904.0
38	2607877.0
39	2910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96493998869867	18.244996993615466	12.386222604771854	25.40384041291402
2	16.419369511270855	20.402185344600106	37.13775951851254	26.04068562561651
3	18.34734733672727	25.261346269252762	29.133668537739467	27.257637856280496
4	13.115688502015525	16.86551235784009	36.28952226435174	33.72927687579265
5	14.297346202293465	37.06943073021118	33.64843594226253	14.98478712523282
6	32.55563299551737	36.28115031531848	17.207830540326	13.955386148838155
7	28.10034235908122	31.394878623708333	22.014341812286027	18.49043720492442
8	27.32711619204313	32.71231289952083	19.92814269772898	20.032428210707064
9	27.400777101855677	14.189134230294838	19.189187277575474	39.22090139027401
10-11	25.586723749003614	25.021937371995158	27.195329476119063	22.19600940288216
12-13	26.60396614114494	22.970438246708486	26.718500660778687	23.707094951367885
14-15	24.49372831522381	23.782405087533455	24.287955873367707	27.43591072387503
16-17	24.046679158621163	26.228296181194278	25.39609996392785	24.328924696256713
18-19	24.39807998768215	25.76014372820641	26.776499020937628	23.065277263173815
20-21	25.218507189674767	25.194788934391667	25.88656967991543	23.700134196018137
22-23	24.822143357697275	24.85918506476195	25.490553531574644	24.82811804596613
24-25	24.43909196748392	25.4259129202379	25.554850025474913	24.580145086803267
26-27	24.6938530468101	25.196187623162935	25.518973931003288	24.590985399023676
28-29	24.30598117249566	25.1474028382483	26.070442072321104	24.476173916934936
30-31	24.880429389172807	25.02167457479204	25.8056045676157	24.292291468419457
32-33	24.199163225331834	25.31657548753084	25.578592094214308	24.905669192923018
34-35	24.47017560181923	25.263852951549914	26.042901463659067	24.223069982971786
36-37	24.87728049023331	25.02614960320613	25.657140548710487	24.439429357850077
38-39	24.928963286836524	24.679358430200597	25.75057176388713	24.64110651907575
40-41	24.53406195835862	24.91770272968128	26.019699113691885	24.528536198268217
42-43	25.102762103893234	24.802766130045818	25.60973768527783	24.48473408078312
44-45	24.809856103130443	25.04174412929826	25.49538679084627	24.65301297672503
46-47	24.429687325726082	24.91684158667409	26.00421562658632	24.649255461013514
48-49	25.09916571849069	24.99668450121636	25.552419906050666	24.35172987424228
50-51	24.2533168142397	25.382610459763683	25.31922205379252	25.044850672204106
52-53	24.136853835507843	25.439496272958973	24.941559932784614	25.482089958748567
54-55	24.37205953102661	25.300835930043412	24.97475153126437	25.352353007665606
56-57	24.68147841243302	24.70611190925515	25.01330970548893	25.599099972822902
58-59	24.328072442696662	24.7058542285648	25.64964048925725	25.316432839481283
60-61	24.44592577595147	25.042121090496817	25.265747891259082	25.24620524229263
62-63	23.973664403342937	24.988042041685446	25.265181710298506	25.773111844673107
64-65	23.72111721344912	24.87727200327211	25.51869759720951	25.882913186069256
66-67	24.470968056095284	24.760266673231847	25.26949766448733	25.499267606185544
68-69	24.43483223185416	24.463930025468137	25.260914409980938	25.84032333269677
70-71	23.81306094826042	24.656024390362504	25.426051105491794	26.10486355588528
72-73	24.529449970245686	24.57948482529967	25.374088242795207	25.516976961659445
74-75	24.552317264087076	24.58521155795238	25.34590868165873	25.516562496301816
76-77	24.094573222200268	24.67809018409241	25.69253871400803	25.534797879699294
78-79	24.203879553078096	24.776930118803747	25.50599051637598	25.513199811742176
80-81	23.78617932605276	24.794217075412206	25.712360823109936	25.707242775425097
82-83	23.905150779016246	24.762874907242967	25.401039205203414	25.930935108537373
84-85	24.093199603170365	24.83733677994121	25.024278168677622	26.045185448210802
86-87	23.851073576129803	24.545029084099852	25.416125580116926	26.187771759653415
88-89	23.961110978488065	24.15985851365477	25.61944313691724	26.259587370939926
90-91	24.113521819318134	24.398743686715623	25.433066986384112	26.05466750758213
92-93	23.830615196395737	24.49743550139878	25.449979584600435	26.221969717605052
94-95	23.18714602691513	24.579282319836096	25.870610138656286	26.36296151459249
96-97	23.911968186722913	24.312966622637227	25.466620936985123	26.308444253654734
98-99	23.744789124817803	24.368893781933785	25.32283690872005	26.563480184528366
100	23.66305152414615	24.422995886479793	25.383379484957132	26.530573104416927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	913.0
1	884.0
2	1119.5
3	1368.5
4	1141.0
5	1016.5
6	1335.5
7	2559.0
8	4244.5
9	4145.5
10	2707.5
11	1992.5
12	2023.0
13	2265.5
14	2707.5
15	3285.0
16	3838.0
17	4468.0
18	5325.0
19	6355.5
20	7582.5
21	9125.5
22	11357.5
23	14238.0
24	17543.0
25	21859.0
26	28999.0
27	37558.5
28	46259.0
29	61881.5
30	81214.5
31	98676.5
32	121243.0
33	143511.0
34	166323.0
35	186209.5
36	205169.5
37	235854.5
38	253918.5
39	261337.5
40	270759.0
41	281003.5
42	299436.0
43	337054.0
44	384713.5
45	430961.0
46	487033.5
47	546528.0
48	616857.5
49	676701.0
50	754041.0
51	765248.5
52	688590.5
53	662817.0
54	654912.5
55	624523.5
56	591952.0
57	544227.5
58	506136.5
59	470494.5
60	403666.5
61	333938.5
62	274588.5
63	219742.5
64	169037.5
65	130871.0
66	100891.5
67	76798.5
68	60844.5
69	50201.0
70	43075.5
71	36915.5
72	30390.0
73	29351.0
74	23703.5
75	17474.0
76	13620.0
77	9461.5
78	6808.5
79	4445.5
80	3075.5
81	2332.0
82	1754.5
83	1269.0
84	890.5
85	567.0
86	392.0
87	238.5
88	135.0
89	98.0
90	63.0
91	40.0
92	29.0
93	24.0
94	19.5
95	7.5
96	4.0
97	5.0
98	2.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08023741242438041
2	6.528896077928904E-4
3	0.0
4	0.0
5	0.06171166980325715
6	0.0
7	2.72037336580371E-5
8	0.0
9	0.0
10-11	5.066695393809409E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.001142556813637558
24-25	0.001247971281562452
26-27	1.2581726816842157E-4
28-29	0.0036350989100552076
30-31	0.0
32-33	1.7002333536273186E-4
34-35	2.0402800243527822E-4
36-37	5.44074673160742E-5
38-39	1.5982193524096794E-4
40-41	3.0604200365291735E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0013193810824147993
50-51	4.828662724301585E-4
52-53	0.006668315212926343
54-55	1.360186682901855E-5
56-57	0.0020946874916688567
58-59	0.001836252021917504
60-61	8.535171435209139E-4
62-63	0.0018158492216739763
64-65	0.0014281960170469477
66-67	1.7682426877724115E-4
68-69	0.0
70-71	1.5982193524096794E-4
72-73	7.753064092540572E-4
74-75	0.004801458990643548
76-77	0.006457486277076556
78-79	0.0042437824506537875
80-81	0.006746525947193201
82-83	8.637185436426778E-4
84-85	0.002210303359715514
86-87	2.6863686987311635E-4
88-89	0.0036521012435914804
90-91	3.230443371891905E-4
92-93	0.00341746904079091
94-95	0.0016254230860677167
96-97	4.488616053576122E-4
98-99	0.0
100	0.0010609456126634469
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.4703864E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.04700069151805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.88880658267674	23.42662440158607
2	14.253838480075565	9.420932202156873
3	5.458289467983935	5.411402874689123
4	2.7022571963811806	3.5720598174987397
5	1.5254803064228863	2.520627437062714
6	0.9550398463818834	1.8936721478285634
7	0.6693111772896412	1.5483108857112071
8	0.4946064139421194	1.3076206802859585
9	0.3763124463297991	1.1192397906678946
>10	2.260085572061041	14.021499162452155
>50	0.21191076520803726	4.878458321074598
>100	0.16230132991915197	10.810832355442566
>500	0.0229478037189115	5.349696417622299
>1k	0.017617517501259415	10.725185292639422
>5k	8.860180455228903E-4	1.8181734618079703
>10k+	3.0907606239170593E-4	2.175664751473634
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	61516	0.41836621992695255	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	58821	0.4000377043748501	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	23546	0.16013477817803537	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	19672	0.13378796213022645	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	18337	0.12470871602185657	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18236	0.12402182174699114	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	17591	0.11963521969463266	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	17085	0.11619394738689094	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0402800243527824E-5
2	0.0	0.0	0.0	0.0	2.0402800243527824E-5
3	0.0	0.0	0.0	6.800933414509274E-6	2.0402800243527824E-5
4	0.0	0.0	0.0	2.0402800243527824E-5	2.7203733658037097E-5
5	0.0	0.0	0.0	2.0402800243527824E-5	2.7203733658037097E-5
6	0.0	0.0	0.0	2.0402800243527824E-5	3.400466707254637E-5
7	0.0	6.800933414509274E-6	0.0	2.0402800243527824E-5	3.400466707254637E-5
8	0.0	6.800933414509274E-6	0.0	2.0402800243527824E-5	3.400466707254637E-5
9	0.0	6.800933414509274E-6	0.0	2.7203733658037097E-5	6.120840073058347E-5
10-11	3.400466707254637E-6	6.800933414509274E-6	0.0	5.780793402332884E-5	6.120840073058347E-5
12-13	6.800933414509274E-6	1.020140012176391E-5	0.0	8.841213438862057E-5	6.120840073058347E-5
14-15	6.800933414509274E-6	1.3601866829018548E-5	0.0	1.1561586804665768E-4	1.1221540133940303E-4
16-17	1.020140012176391E-5	1.3601866829018548E-5	0.0	1.9042613560625967E-4	1.4281960170469475E-4
18-19	2.0402800243527824E-5	1.3601866829018548E-5	0.0	2.108289358497875E-4	1.5982193524096794E-4
20-21	3.400466707254637E-5	2.7203733658037097E-5	0.0	3.128429370674266E-4	1.7342380206998651E-4
22-23	3.400466707254637E-5	2.7203733658037097E-5	0.0	8.365148099846408E-4	1.904261356062597E-4
24-25	4.760653390156492E-5	2.7203733658037097E-5	0.0	0.0020096758239874905	2.108289358497875E-4
26-27	6.800933414509275E-5	2.7203733658037097E-5	0.0	0.003852728779319504	2.108289358497875E-4
28-29	6.800933414509275E-5	2.7203733658037097E-5	0.0	0.017083944737247298	2.108289358497875E-4
30-31	6.800933414509275E-5	3.400466707254637E-5	0.0	0.059266734240741074	2.1422940255704214E-4
32-33	6.800933414509275E-5	3.400466707254637E-5	0.0	0.13412460833424467	2.2443080267880606E-4
34-35	8.16112009741113E-5	4.080560048705565E-5	0.0	0.2321804663046394	2.2443080267880606E-4
36-37	8.16112009741113E-5	5.780793402332884E-5	0.0	0.38408611505111856	2.3123173609331535E-4
38-39	8.16112009741113E-5	6.800933414509275E-5	0.0	0.6314530656703572	2.482340696295885E-4
40-41	8.841213438862057E-5	6.800933414509275E-5	0.0	0.9354343864986783	2.652364031658617E-4
42-43	8.841213438862057E-5	7.140980085234739E-5	0.0	1.184899425076293	2.754378032876256E-4
44-45	8.841213438862057E-5	7.481026755960202E-5	0.0	1.4642477650772614	2.890396701166442E-4
46-47	8.841213438862057E-5	7.481026755960202E-5	0.0	1.7836536028896894	2.9584060353115345E-4
48-49	8.841213438862057E-5	7.481026755960202E-5	0.0	2.110074603519184	3.09442470360172E-4
50-51	8.841213438862057E-5	7.481026755960202E-5	0.0	2.450804768052806	3.196438704819359E-4
52-53	8.841213438862057E-5	7.481026755960202E-5	0.0	2.7850264393087425	3.4004667072546377E-4
54-55	8.841213438862057E-5	8.16112009741113E-5	0.0	3.118282378019819	3.638499376762462E-4
56-57	8.841213438862057E-5	8.501166768136594E-5	0.0	3.489660948985926	3.774518045052647E-4
58-59	8.841213438862057E-5	8.841213438862057E-5	0.0	3.906728190630708	3.8765320462702866E-4
60-61	8.841213438862057E-5	8.841213438862057E-5	0.0	4.323870242543048	3.8765320462702866E-4
62-63	9.18126010958752E-5	8.841213438862057E-5	0.0	4.741893015332568	3.8765320462702866E-4
64-65	9.521306780312985E-5	8.841213438862057E-5	0.0	5.170433431647627	4.012550714560472E-4
66-67	9.521306780312985E-5	8.841213438862057E-5	0.0	5.62547368501232	4.0465553816330187E-4
68-69	9.521306780312985E-5	8.841213438862057E-5	0.0	6.142535050650632	4.080560048705565E-4
70-71	9.521306780312985E-5	9.521306780312985E-5	0.0	6.7395413885765	4.284588051140843E-4
72-73	1.0201400121763912E-4	1.0201400121763912E-4	0.0	7.303359171439562	5.100700060881955E-4
74-75	1.0201400121763912E-4	1.0201400121763912E-4	0.0	7.850395651102323	5.100700060881955E-4
76-77	1.0201400121763912E-4	1.0201400121763912E-4	0.0	8.434534623007938	5.134704727954502E-4
78-79	1.0881493463214839E-4	1.0541446792489376E-4	0.0	9.044537544688932	5.202714062099595E-4
80-81	1.0881493463214839E-4	1.0881493463214839E-4	0.0	9.711273852913763	5.338732730389781E-4
82-83	1.1561586804665766E-4	1.1901633475391231E-4	0.0	10.434675538348287	5.576765399897605E-4
84-85	1.2241680146116694E-4	1.3261820158293086E-4	0.0	11.123933137575266	5.644774734042697E-4
86-87	1.2241680146116694E-4	1.360186682901855E-4	0.0	11.821107023296733	5.644774734042697E-4
88	1.2241680146116694E-4	1.360186682901855E-4	0.0	12.371911220071132	5.644774734042697E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAAG	5515	0.0	20.623722	1
TAGTACT	5760	0.0	19.337461	4
CATGGGG	35185	0.0	18.619963	4
GTACTAG	5185	0.0	17.585318	1
TAATACG	2580	0.0	17.123074	4
ATTTAGG	7490	0.0	16.754314	1
CTATTGA	6985	0.0	16.618973	9
TCTAACG	2730	0.0	16.354675	2
GTATATA	3320	0.0	16.280096	1
GGGCTAT	8170	0.0	16.279385	6
TTTAGGG	7915	0.0	16.210361	2
CTAGTAC	7090	0.0	16.173935	3
GTGATCG	4290	0.0	15.994445	8
CTAACGC	2830	0.0	15.77645	3
GTACATA	3715	0.0	15.68773	1
GTACATG	132250	0.0	15.462889	1
GTCCTAC	5215	0.0	15.411292	1
TAGGCAT	7460	0.0	15.371796	5
CCTACAC	6570	0.0	15.308072	3
AATACGG	2405	0.0	15.242393	5
>>END_MODULE