FastQCFastQC Report
Fri 27 May 2016
ERR765406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13904246
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178010.1280256405129771No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT149840.10776564223619173No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA146410.1052987698865512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA464800.022.5938911
GTACATG706500.019.8146571
GTACTAG42100.019.2052671
TACATGG709000.019.151872
TAGTACT52450.018.2794
AACGCAG520500.018.1730046
ACATGGG689750.017.7844943
CATGGGG297600.017.7027384
CAACGCA529750.017.5547925
TATCAAC596050.017.4741882
ATCAACG533650.017.4646113
GTATAAG52750.017.1992231
TCAACGC557550.016.715014
ACGCAGA555000.016.602117
GTACAAG76950.016.494121
CTAGTAC60550.016.3780063
CGCAGAG556550.015.8623848
CATGGGT100550.015.6577914
GTATTAG61450.015.376171
GTCCTAT75350.015.3470831
Produced by FastQC (version 0.11.5)