##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765406_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13904246
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59258970245492	33.0	33.0	33.0	27.0	33.0
2	31.49833453752185	33.0	33.0	33.0	27.0	33.0
3	31.426781071048367	33.0	33.0	33.0	27.0	33.0
4	35.042424666537116	37.0	37.0	37.0	33.0	37.0
5	35.266530813680944	37.0	37.0	37.0	33.0	37.0
6	35.259705848127254	37.0	37.0	37.0	33.0	37.0
7	35.12088062883813	37.0	37.0	37.0	33.0	37.0
8	35.112495276622695	37.0	37.0	37.0	33.0	37.0
9	34.81208215102063	37.0	37.0	37.0	33.0	37.0
10-11	34.81021344127542	37.0	37.0	37.0	30.0	37.0
12-13	34.7924888196023	37.0	37.0	37.0	33.0	37.0
14-15	36.042433045272645	37.0	37.0	40.0	30.0	40.0
16-17	36.28737088656227	37.0	37.0	40.0	33.0	40.0
18-19	36.09238145671473	37.0	37.0	40.0	27.0	40.0
20-21	36.08366631315355	37.0	37.0	40.0	27.0	40.0
22-23	36.24963137159685	37.0	37.0	40.0	30.0	40.0
24-25	36.26328863859284	37.0	37.0	40.0	33.0	40.0
26-27	35.93677071737655	37.0	37.0	40.0	27.0	40.0
28-29	35.81160851872155	37.0	37.0	40.0	27.0	40.0
30-31	35.621581529843475	37.0	37.0	40.0	27.0	40.0
32-33	35.58897638174699	37.0	35.0	40.0	27.0	40.0
34-35	35.53432224947689	37.0	35.0	40.0	27.0	40.0
36-37	35.3000930794809	37.0	33.0	40.0	27.0	40.0
38-39	35.161598262861574	37.0	33.0	40.0	27.0	40.0
40-41	35.312764640383946	37.0	33.0	40.0	27.0	40.0
42-43	35.23639890289628	37.0	33.0	40.0	27.0	40.0
44-45	35.19597732951503	37.0	33.0	40.0	27.0	40.0
46-47	35.1668605762585	37.0	33.0	40.0	27.0	40.0
48-49	35.009318232718265	37.0	33.0	40.0	27.0	40.0
50-51	34.85781418855794	37.0	33.0	40.0	27.0	40.0
52-53	34.73924472423748	37.0	33.0	40.0	27.0	40.0
54-55	34.60745936888631	37.0	33.0	40.0	27.0	40.0
56-57	34.40547962830922	37.0	33.0	40.0	27.0	40.0
58-59	34.218318670426285	37.0	33.0	38.5	27.0	40.0
60-61	33.950296585661675	37.0	33.0	37.0	27.0	40.0
62-63	33.7328785034442	37.0	33.0	37.0	27.0	40.0
64-65	33.53067886600971	37.0	33.0	37.0	27.0	40.0
66-67	33.30379583330157	37.0	33.0	37.0	24.5	40.0
68-69	33.10632079582021	37.0	33.0	37.0	22.0	40.0
70-71	32.876609454406946	37.0	33.0	37.0	22.0	37.0
72-73	32.64219764236047	37.0	33.0	37.0	22.0	37.0
74-75	32.42308845801492	33.0	33.0	37.0	22.0	37.0
76-77	32.226724663818516	33.0	33.0	37.0	22.0	37.0
78-79	32.03970887741774	33.0	33.0	37.0	22.0	37.0
80-81	31.880890556740724	33.0	33.0	37.0	22.0	37.0
82-83	31.73891158858956	33.0	33.0	37.0	22.0	37.0
84-85	31.571482013479912	33.0	33.0	37.0	22.0	37.0
86-87	31.368681408542397	33.0	33.0	37.0	22.0	37.0
88-89	31.221978271960953	33.0	27.0	37.0	22.0	37.0
90-91	31.1286554121669	33.0	27.0	37.0	22.0	37.0
92-93	31.037039656806993	33.0	27.0	37.0	22.0	37.0
94-95	30.893699126151823	33.0	27.0	37.0	15.0	37.0
96-97	30.786053195549044	33.0	27.0	37.0	15.0	37.0
98-99	30.646523551151212	33.0	27.0	37.0	15.0	37.0
100	30.563143373614075	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	10.0
7	317.0
8	1905.0
9	4359.0
10	6464.0
11	10616.0
12	17675.0
13	26951.0
14	36796.0
15	42548.0
16	49033.0
17	57361.0
18	67778.0
19	80490.0
20	95407.0
21	110836.0
22	128831.0
23	139174.0
24	147660.0
25	165018.0
26	190597.0
27	228290.0
28	279260.0
29	346590.0
30	430148.0
31	532511.0
32	680000.0
33	886589.0
34	1158679.0
35	1558130.0
36	2273938.0
37	3042143.0
38	1107245.0
39	895.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.125598755581365	18.618033622271053	12.852566930026779	25.403800692120797
2	16.111443903863044	20.600987980830272	37.410243972376705	25.877324142929975
3	17.86152324431256	25.499987411204028	29.40471540329107	27.23377394119234
4	12.804879934917704	17.03697724989891	36.65052917492047	33.50761364026292
5	14.563839169692761	36.78526694936193	33.310713299996955	15.340180580948346
6	32.156268252111445	36.54610540452056	17.029457824300476	14.268168519067515
7	28.02945100611007	31.534225213288313	21.9744993770771	18.461824403524517
8	27.24487078921525	32.60864999554786	20.01777837747096	20.12870083776594
9	26.88359291327239	14.457984184188346	19.732040745181077	38.926382157358184
10-11	25.455532484216477	24.795012764514723	27.142704617107533	22.606750134161267
12-13	26.33732709242622	22.630340177049614	26.716057965335832	24.316274765188332
14-15	24.177854082488416	23.843408435623854	24.315168038961147	27.663569442926583
16-17	24.092932281409187	26.25097185216907	25.04540584679643	24.61069001962531
18-19	24.373216048505856	25.39296830468642	26.889414337063215	23.344401309744505
20-21	25.48196778192684	24.843785558861956	25.970247149464964	23.70399950974624
22-23	24.82996189644494	24.56032007849462	25.980377064781802	24.629340960278647
24-25	24.42616761086476	25.14186351643255	25.978586602380698	24.453382270321995
26-27	24.523383673494497	25.094486946429296	26.069181520079514	24.312947859996694
28-29	24.132968621827768	24.953155778536875	26.554912817743915	24.358962781891442
30-31	24.613516368236024	24.909895195513684	26.2842434952289	24.192344941021393
32-33	24.250340025633545	25.033056423839422	25.780326766369626	24.936276784157407
34-35	24.747523117450537	24.98617018444311	26.211350090771656	24.054956607334695
36-37	24.72078321512477	24.95681899407342	25.88100584743313	24.441391943368682
38-39	24.700871239455278	24.66254151038058	26.023317053962558	24.613270196201583
40-41	24.568267320172712	25.026670442558263	26.16028343221063	24.244778805058395
42-43	25.000780790678295	24.914210174458145	25.79985348480912	24.28515555005444
44-45	24.501483270706267	25.194822867645666	25.495035548878885	24.808658312769182
46-47	24.352704467909646	25.143922813976268	25.790119066869927	24.71325365124416
48-49	24.988545480604213	25.32070405325361	25.193918995092574	24.4968314710496
50-51	24.328682143549354	25.60703149157748	25.09638817899258	24.967898185880582
52-53	24.46707029763124	25.312490953724243	25.102946393365606	25.117492355278912
54-55	24.691828837458317	25.166550748834233	25.24152425243067	24.90009616127678
56-57	24.65215301858152	24.94456369658592	24.954542662723313	25.448740622109245
58-59	24.353280285748685	24.950968215033022	25.470611639063346	25.22513986015495
60-61	24.441149128115253	25.15505695166786	25.299645877956994	25.1041480422599
62-63	24.095587610216967	25.187361111770386	25.140907408614083	25.576143869398564
64-65	24.175590157203363	24.81903514321804	25.290579068123137	25.714795631455463
66-67	24.551990279477256	24.91586259552471	25.17952411540967	25.35262300958837
68-69	24.538223591751198	24.584965366746907	25.19628362972358	25.680527411778314
70-71	23.97005862529108	24.631274292053977	25.47032782379013	25.928339258864813
72-73	24.4836092691417	24.72807137411717	25.325520346370162	25.46279901037097
74-75	24.579857308937072	24.63044621406522	25.31368051941299	25.476015957584718
76-77	24.2732597497229	24.808230100648878	25.35638349154	25.562126658088218
78-79	24.307167498385116	24.81540438964519	25.34731750437789	25.530110607591805
80-81	24.015211190113806	24.64510549391867	25.656660424564816	25.68302289140271
82-83	24.16479289447172	24.787452956714713	25.327677590739384	25.720076558074183
84-85	24.141706044580317	24.864369559072014	25.096445816274905	25.897478580072764
86-87	24.002998925605556	24.61316261935898	25.295703012001315	26.08813544303415
88-89	23.98563058321679	24.338497256332513	25.443922486134223	26.231949674316475
90-91	24.252065051412043	24.443083251022806	25.265061711497466	26.039789986067685
92-93	24.043998883460347	24.541004314615904	25.224877660007923	26.19011914191583
94-95	23.537536490878725	24.52823925976586	25.64898185872268	26.285242390632728
96-97	24.0735574318658	24.449135939281426	25.27479371951032	26.202512909342456
98-99	23.682131363736534	24.455393787979308	25.384727032319056	26.477747815965103
100	23.72581181798292	24.52542090310026	25.206709765694168	26.54205751322265
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1020.0
1	1018.0
2	1447.5
3	1865.0
4	1580.0
5	1451.0
6	1945.0
7	3493.0
8	5615.5
9	5722.0
10	3980.0
11	2862.5
12	2652.0
13	2827.0
14	3205.5
15	3619.0
16	4153.5
17	4779.5
18	5642.0
19	6764.5
20	8087.5
21	10170.0
22	13455.5
23	17332.5
24	21481.5
25	26949.5
26	35620.0
27	46071.5
28	55191.5
29	68796.0
30	85605.0
31	99623.0
32	116742.0
33	136498.0
34	157644.0
35	175890.5
36	193981.0
37	217288.5
38	235660.0
39	251312.0
40	263560.0
41	275638.5
42	293802.5
43	329119.5
44	373046.0
45	413991.5
46	468274.5
47	517874.5
48	565040.5
49	602997.0
50	625352.5
51	623958.5
52	610634.5
53	617619.5
54	609998.5
55	584662.0
56	557995.5
57	520413.0
58	493588.0
59	458912.0
60	396391.0
61	328383.5
62	270619.5
63	215976.5
64	164870.0
65	132451.5
66	105240.0
67	79843.0
68	62666.0
69	51822.0
70	45601.5
71	39916.5
72	33945.5
73	33119.0
74	27342.5
75	20417.0
76	15254.0
77	10683.0
78	7767.5
79	5034.5
80	3461.0
81	2538.0
82	1774.0
83	1309.5
84	969.5
85	661.5
86	458.5
87	270.0
88	161.0
89	101.0
90	55.0
91	38.0
92	27.5
93	20.0
94	15.0
95	11.0
96	8.0
97	5.0
98	3.0
99	3.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04798534203149168
2	0.024230008588743326
3	0.021547374809105072
4	0.022129930670098902
5	0.01521118081483886
6	0.024862908783403286
7	0.04933744699281068
8	0.006041320039936002
9	0.004063506931623621
10-11	0.0019454488938127246
12-13	0.00127658846081981
14-15	0.002463276325807239
16-17	0.007375444882088536
18-19	0.005545068750941259
20-21	0.008932523201905376
22-23	0.019102078602464313
24-25	0.026247377959221954
26-27	0.029095428835191783
28-29	0.04545733727668513
30-31	0.04305159733221061
32-33	0.040717777864402
34-35	0.048445633082153466
36-37	0.045691078825849316
38-39	0.055536992081411676
40-41	0.052555888323609926
42-43	0.05808297695538471
44-45	0.0582376059802164
46-47	0.06312460236966463
48-49	0.06527142859814189
50-51	0.03394646498630706
52-53	3.1285407349668584E-4
54-55	3.5960238332952396E-6
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.753638133272383E-4
64-65	1.9418528699794292E-4
66-67	3.9915864549577157E-4
68-69	0.0014060453188184387
70-71	5.7536381332723834E-5
72-73	3.30834192663162E-4
74-75	8.27085481657905E-5
76-77	1.258608341653334E-4
78-79	1.0788071499885718E-5
80-81	6.113240516601907E-5
82-83	2.1576142999771438E-4
84-85	0.0014060453188184387
86-87	0.004315228599954288
88-89	0.002955931590968687
90-91	0.004293652456954516
92-93	0.004135427408289525
94-95	0.0030853884489673157
96-97	0.0026035212553057532
98-99	0.004473453648619278
100	0.003250805545298896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3904246E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.26277269884441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.51473317371556	29.256550313146352
2	12.51434442222305	9.8269572104959
3	4.7847633863297245	5.635892317656511
4	2.342987348123975	3.6796871874263957
5	1.3269550123746567	2.6049966516229213
6	0.868926410676528	2.046987609264915
7	0.5944340519214225	1.6337390345530722
8	0.42912648401872533	1.3478956480865203
9	0.33067448739546257	1.1684877512323444
>10	1.9694954576398922	14.534729955938145
>50	0.1772794262909565	4.82174394396996
>100	0.12043958402274949	9.352823140396326
>500	0.014328383108480522	3.8980929248879295
>1k	0.010903458069220648	8.37035524940454
>5k	5.327998286120844E-4	1.3883399684633162
>10k+	7.611426123029777E-5	0.4327210934548091
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17801	0.1280256405129771	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	14984	0.10776564223619173	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	14641	0.1052987698865512	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.315228599954287E-5
2	0.0	7.192047666590479E-6	0.0	0.0	4.315228599954287E-5
3	0.0	7.192047666590479E-6	0.0	0.0	4.315228599954287E-5
4	0.0	7.192047666590479E-6	0.0	0.0	4.315228599954287E-5
5	7.192047666590479E-6	2.1576142999771436E-5	0.0	2.8768190666361917E-5	4.315228599954287E-5
6	1.4384095333180958E-5	2.1576142999771436E-5	0.0	2.8768190666361917E-5	5.034433366613335E-5
7	1.4384095333180958E-5	2.1576142999771436E-5	0.0	3.5960238332952394E-5	5.034433366613335E-5
8	1.4384095333180958E-5	2.1576142999771436E-5	0.0	3.5960238332952394E-5	5.034433366613335E-5
9	1.4384095333180958E-5	5.7536381332723834E-5	0.0	3.5960238332952394E-5	5.7536381332723834E-5
10-11	2.1576142999771436E-5	5.7536381332723834E-5	0.0	3.955626216624763E-5	6.472842899931431E-5
12-13	2.5172166833066678E-5	5.7536381332723834E-5	0.0	5.034433366613335E-5	2.1576142999771438E-4
14-15	2.8768190666361917E-5	6.472842899931431E-5	0.0	5.7536381332723834E-5	2.5891371599725724E-4
16-17	2.8768190666361917E-5	6.472842899931431E-5	0.0	8.630457199908574E-5	2.9487395433020966E-4
18-19	4.315228599954287E-5	6.832445283260955E-5	0.0	1.006886673322667E-4	3.056620258300954E-4
20-21	4.674830983283811E-5	7.911252433249527E-5	0.0	2.9487395433020966E-4	3.919665978291811E-4
22-23	5.034433366613335E-5	7.911252433249527E-5	0.0	0.001107575340654934	4.243308123288383E-4
24-25	6.113240516601907E-5	8.27085481657905E-5	0.0	0.0025028325879734867	4.387149076620192E-4
26-27	8.630457199908574E-5	8.630457199908574E-5	0.0	0.004390745100453487	4.6029105066179067E-4
28-29	8.990059583238098E-5	8.630457199908574E-5	0.0	0.014387691357014253	4.674830983283811E-4
30-31	9.349661966567622E-5	8.630457199908574E-5	0.0	0.04378159017036954	4.710791221616764E-4
32-33	9.349661966567622E-5	8.630457199908574E-5	0.0	0.0922991437291889	4.890592413281526E-4
34-35	9.349661966567622E-5	9.349661966567622E-5	0.0	0.15616812303234567	5.250194796611049E-4
36-37	9.709264349897146E-5	1.006886673322667E-4	0.0	0.25604049295445436	5.250194796611049E-4
38-39	1.006886673322667E-4	1.0788071499885719E-4	0.0	0.40417509874321844	5.358075511609907E-4
40-41	1.006886673322667E-4	1.1507276266544767E-4	0.0	0.5755615946380696	5.465956226608764E-4
42-43	1.006886673322667E-4	1.1507276266544767E-4	0.0	0.7351998806695451	5.53787670327467E-4
44-45	1.0788071499885719E-4	1.2226481033203814E-4	0.0	0.9273785863685093	5.897479086604193E-4
46-47	1.1147673883215243E-4	1.3305288183192387E-4	0.0	1.1487641976414973	6.221121231600765E-4
48-49	1.1507276266544767E-4	1.366489056652191E-4	0.0	1.3763385659315865	6.61668385326324E-4
50-51	1.1507276266544767E-4	1.8699323933135244E-4	0.0	1.619397412847845	7.263968143256384E-4
52-53	1.1507276266544767E-4	1.8699323933135244E-4	0.0	1.8595902287689676	7.65953076491886E-4
54-55	1.1507276266544767E-4	1.9778131083123814E-4	0.0	2.107205237882011	7.94721267158248E-4
56-57	1.1507276266544767E-4	2.265495014976001E-4	0.0	2.3921289942654926	8.198934339913146E-4
58-59	1.1507276266544767E-4	2.3374154916419056E-4	0.0	2.6946732674321208	8.486616246576765E-4
60-61	1.1507276266544767E-4	2.373375729974858E-4	0.0	3.005689772749993	8.666417438241527E-4
62-63	1.1507276266544767E-4	2.445296206640763E-4	0.0	3.319759302302333	9.205821013235813E-4
64-65	1.1507276266544767E-4	2.5891371599725724E-4	0.0	3.6518413152356484	9.277741489901718E-4
66-67	1.186687864987429E-4	2.6250973983055247E-4	0.0	4.021555717584398	9.313701728234671E-4
68-69	1.2945685799862862E-4	2.732978113304382E-4	0.0	4.412249322976592	9.349661966567622E-4
70-71	1.3305288183192387E-4	2.8408588283032393E-4	0.0	4.844149765474517	9.529463158232385E-4
72-73	1.4024492949851432E-4	2.8768190666361915E-4	0.0	5.272461376186814	0.0010032906494893718
74-75	1.4384095333180958E-4	2.984699781635049E-4	0.0	5.701855390072932	0.001021270768655848
76-77	1.4384095333180958E-4	3.0925804966339056E-4	0.0	6.161247434776398	0.001035654863989029
78-79	1.4743697716510483E-4	3.1645009732998106E-4	0.0	6.6448227397587765	0.0010428469116556195
80-81	1.5103300099840005E-4	3.30834192663162E-4	0.0	7.162970217874454	0.0010716151023219813
82-83	1.5103300099840005E-4	3.30834192663162E-4	0.0	7.706656657254195	0.0010931912453217528
84-85	1.5103300099840005E-4	3.30834192663162E-4	0.0	8.243787545185837	0.0010931912453217528
86-87	1.5103300099840005E-4	3.380262403297525E-4	0.0	8.805544004327887	0.001107575340654934
88	1.5103300099840005E-4	3.524103356629335E-4	0.0	9.244104282965074	0.0011219594359881148
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	46480	0.0	22.593891	1
GTACATG	70650	0.0	19.814657	1
GTACTAG	4210	0.0	19.205267	1
TACATGG	70900	0.0	19.15187	2
TAGTACT	5245	0.0	18.279	4
AACGCAG	52050	0.0	18.173004	6
ACATGGG	68975	0.0	17.784494	3
CATGGGG	29760	0.0	17.702738	4
CAACGCA	52975	0.0	17.554792	5
TATCAAC	59605	0.0	17.474188	2
ATCAACG	53365	0.0	17.464611	3
GTATAAG	5275	0.0	17.199223	1
TCAACGC	55755	0.0	16.71501	4
ACGCAGA	55500	0.0	16.60211	7
GTACAAG	7695	0.0	16.49412	1
CTAGTAC	6055	0.0	16.378006	3
CGCAGAG	55655	0.0	15.862384	8
CATGGGT	10055	0.0	15.657791	4
GTATTAG	6145	0.0	15.37617	1
GTCCTAT	7535	0.0	15.347083	1
>>END_MODULE