FastQCFastQC Report
Fri 27 May 2016
ERR765405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6752733
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA304470.45088410870087714No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT282240.4179641043115432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200400.2967687305273287No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG166870.24711476079388892No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA166760.24695186378611444No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC165130.24453802630727442No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG128720.1906191167339209No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124930.1850065743751456No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG117650.17422575422425263No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT108330.16042393502008742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101540.15036874699473532No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC101180.1498356295147461No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT98650.14608899833593303No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC87440.12948831236182448No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC84890.1257120635452342No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG76440.11319861158437627No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA71540.10594229032896754No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA71410.10574977568341588No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT70590.10453545253455157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC41550.021.867361
AGTACTT197950.020.26394812-13
TCTACAC57050.019.4568563
GAGTACT190300.019.36213912-13
TACACTC52400.018.9417065
TACTTTT230500.017.63201514-15
GTACTTT214750.017.240514-15
CATGGGG144950.017.0385074
ATCTACA55400.016.8974382
CATGGGT71800.016.8383374
ATGGGTA51950.016.6609675
ACTTTTT241150.015.69643916-17
GTATTAG41950.015.4866381
ATTATAC13300.015.2066333
GTATCAA842150.015.1100761
TATACTG16850.015.0751545
GTACTAG13550.014.59217551
GTCCTAT38550.014.4101221
GTATAGA11550.014.26579951
CTACACT80750.014.0981894
Produced by FastQC (version 0.11.5)