FastQCFastQC Report
Fri 27 May 2016
ERR765405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765405_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6752733
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA319720.47346755750597574No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT282170.41786044257932303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184130.272674782195594No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG175190.2594356981091952No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA170410.25235708268044954No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC164500.2436050707172933No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG147990.2191557107322324No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG129230.19137436649723896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125490.1858358682329066No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT118910.17609166540421486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106540.15777315643902995No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT100240.1484436005392187No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC99280.14702195392591416No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC96080.14228313188156558No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC91790.13593014857836078No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA80160.11870749221093149No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA75550.11188062670329182No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT74220.10991105379110948No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA70460.1043429378889999No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG70300.10410599678678248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTT201500.019.2519712-13
TCCGATC11450.018.4696853
GAGTACT193900.017.9222912-13
GATCTAC22350.017.250731
TACTTTT229600.016.87531714-15
GTACTTT212550.016.41604414-15
TACACTC27050.016.3308895
TCTACAC34400.015.847183
CATGGGG153400.015.3789824
CATGGGT71800.015.1194774
ATTGCGT7950.014.77811056
ACTTTTT238150.014.73049216-17
GTACTAG11500.014.7189181
TAGTACT15100.014.6275024
TACGGGC9200.014.3027474
GTATACG12050.014.04711
ATGGGTA53850.013.9631535
CGCTATA2050.00666009713.7599572
GTATCAA877100.013.6805251
GTCCTAT43300.013.4649581
Produced by FastQC (version 0.11.5)