FastQCFastQC Report
Fri 27 May 2016
ERR765403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7367097
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA175810.2386421680072897No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT165200.22424029437918355No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC79470.10787152659996198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG32100.026.7983631
GTATAAG38400.022.886121
GTGATCG26500.022.8085568
TAGTACT40400.022.4428044
TAGGCAT49350.020.9165335
TATTCGC6850.020.3631869
GTACATA16300.019.9688011
TGATCGC29650.019.915639
CTAGGCA53300.019.8026144
CGTTAAC9200.019.711441
CTAGTAC47950.019.6856163
GTATCAA359350.019.4354171
GTACAAG32450.019.2013451
ATAAGGT33600.018.8209363
TACGGGC27450.018.6326314
CTGTGCG49150.018.4470149
ATACGGG27800.018.39883
GTACAAA33350.018.1254631
GGCGTCG29600.018.0636888
GTACTGT53550.018.0595596
Produced by FastQC (version 0.11.5)