##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765403_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7367097 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.377932718952934 33.0 33.0 33.0 27.0 33.0 2 31.34347939222193 33.0 33.0 33.0 27.0 33.0 3 31.290008941106652 33.0 33.0 33.0 27.0 33.0 4 34.87978507680841 37.0 37.0 37.0 33.0 37.0 5 34.99596286026911 37.0 37.0 37.0 33.0 37.0 6 34.92747916852459 37.0 37.0 37.0 33.0 37.0 7 34.83476693737031 37.0 37.0 37.0 33.0 37.0 8 34.81675332902499 37.0 37.0 37.0 33.0 37.0 9 34.6723637546784 37.0 37.0 37.0 27.0 37.0 10-11 34.680488936144044 37.0 37.0 37.0 27.0 37.0 12-13 34.60283168526219 37.0 37.0 37.0 27.0 37.0 14-15 36.03667855873216 37.0 37.0 40.0 27.0 40.0 16-17 36.09765725902618 37.0 37.0 40.0 27.0 40.0 18-19 36.11082377495505 37.0 37.0 40.0 27.0 40.0 20-21 36.124132409278715 37.0 37.0 40.0 27.0 40.0 22-23 36.12358300698362 37.0 37.0 40.0 27.0 40.0 24-25 36.19283172191163 37.0 37.0 40.0 27.0 40.0 26-27 36.14728759510021 37.0 37.0 40.0 27.0 40.0 28-29 36.166546741545545 37.0 37.0 40.0 30.0 40.0 30-31 36.083253620795276 37.0 37.0 40.0 27.0 40.0 32-33 36.001381073168986 37.0 37.0 40.0 27.0 40.0 34-35 35.862895181100505 37.0 37.0 40.0 27.0 40.0 36-37 35.664812272731034 37.0 37.0 40.0 27.0 40.0 38-39 35.37592046093597 37.0 33.0 40.0 27.0 40.0 40-41 35.48163034910495 37.0 33.0 40.0 27.0 40.0 42-43 35.40473309907553 37.0 33.0 40.0 27.0 40.0 44-45 35.30145415487267 37.0 33.0 40.0 27.0 40.0 46-47 35.20087199883482 37.0 33.0 40.0 27.0 40.0 48-49 35.060798846547016 37.0 33.0 40.0 27.0 40.0 50-51 34.87956090438337 37.0 33.0 40.0 27.0 40.0 52-53 34.73400506332413 37.0 33.0 40.0 27.0 40.0 54-55 34.541860450595394 37.0 33.0 40.0 27.0 40.0 56-57 34.33008822878266 37.0 33.0 38.5 27.0 40.0 58-59 34.10159592034691 37.0 33.0 37.0 27.0 40.0 60-61 33.88723278653722 37.0 33.0 37.0 27.0 40.0 62-63 33.65366887391329 37.0 33.0 37.0 27.0 40.0 64-65 33.4269096769053 37.0 33.0 37.0 27.0 40.0 66-67 33.21160546684807 37.0 33.0 37.0 24.5 40.0 68-69 33.00460330575259 37.0 33.0 37.0 22.0 40.0 70-71 32.76978686448678 37.0 33.0 37.0 22.0 37.0 72-73 32.43359385657607 33.0 33.0 37.0 22.0 37.0 74-75 32.19219103535626 33.0 33.0 37.0 22.0 37.0 76-77 32.01221213729099 33.0 33.0 37.0 22.0 37.0 78-79 31.76301554058539 33.0 33.0 37.0 22.0 37.0 80-81 31.57030768021651 33.0 33.0 37.0 22.0 37.0 82-83 31.440520058307904 33.0 27.0 37.0 22.0 37.0 84-85 31.298952694663853 33.0 27.0 37.0 22.0 37.0 86-87 31.038472277479176 33.0 27.0 37.0 22.0 37.0 88-89 30.90255476478727 33.0 27.0 37.0 22.0 37.0 90-91 30.744021491776202 33.0 27.0 37.0 15.0 37.0 92-93 30.489548189741498 33.0 27.0 37.0 15.0 37.0 94-95 30.341282597473604 33.0 27.0 37.0 15.0 37.0 96-97 30.14693392797733 33.0 27.0 37.0 15.0 37.0 98-99 29.07737769707661 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3038306328085909 1101 2 0.32025795506912047 1101 3 0.31372981689141 1101 4 0.40377476012474034 1101 5 0.34228659214463164 1101 6 0.40475019733013795 1101 7 0.34179755016088365 1101 8 0.4292695084087228 1101 9 0.34559417678762117 1101 10-11 0.35347350735485605 1101 12-13 0.4670242826697546 1101 14-15 0.6044118272792076 1101 16-17 0.506405573473657 1101 18-19 0.47408350409872213 1101 20-21 0.4373412057121513 1101 22-23 0.41239676369033873 1101 24-25 0.3270974723961828 1101 26-27 0.43381357371696083 1101 28-29 0.3888854989532007 1101 30-31 0.5879587988340376 1101 32-33 0.6620587649919116 1101 34-35 0.6699144888516173 1101 36-37 0.7730418317903798 1101 38-39 0.8195064860578398 1101 40-41 0.6628227437699081 1101 42-43 0.529547111337564 1101 44-45 0.38397718256586444 1101 46-47 0.4330869500023553 1101 48-49 0.36263254704937253 1101 50-51 0.25265290373650373 1101 52-53 0.43262400110906185 1101 54-55 0.28478176126285604 1101 56-57 0.4271543965317406 1101 58-59 0.4071829562872793 1101 60-61 0.24329131298149775 1101 62-63 0.3788937203066425 1101 64-65 0.39724683068810407 1101 66-67 0.36385555250910784 1101 68-69 0.5197993279165658 1101 70-71 0.562998899543075 1101 72-73 0.3272632239525848 1101 74-75 0.2984095466692054 1101 76-77 0.36621381294799704 1101 78-79 0.3887350167017516 1101 80-81 0.39548468383646096 1101 82-83 0.4787767980843469 1101 84-85 0.4216185657606353 1101 86-87 0.3722722129045266 1101 88-89 0.23511948526218873 1101 90-91 0.2096092237460887 1101 92-93 0.29443931089791064 1101 94-95 0.3963613250707567 1101 96-97 0.30029123961042714 1101 98-99 0.24725796537560285 1102 1 0.2468499835279765 1102 2 0.3031551726530708 1102 3 0.2605688686262546 1102 4 0.2935440791226185 1102 5 0.24497561134494106 1102 6 0.29851327745387124 1102 7 0.3298185742564357 1102 8 0.39542204341034193 1102 9 0.339983901631534 1102 10-11 0.3120629743682244 1102 12-13 0.430837106582338 1102 14-15 0.5178471277658545 1102 16-17 0.39772702217091904 1102 18-19 0.35746322597174185 1102 20-21 0.3419243396198297 1102 22-23 0.36285079692675026 1102 24-25 0.24892842215144384 1102 26-27 0.37043726707074853 1102 28-29 0.2935697951475831 1102 30-31 0.4353598918732189 1102 32-33 0.6105911854388992 1102 34-35 0.641290393247786 1102 36-37 0.7497220212490774 1102 38-39 0.7145335449702799 1102 40-41 0.49189907661302357 1102 42-43 0.37692611677767474 1102 44-45 0.3372313635738493 1102 46-47 0.324146304741582 1102 48-49 0.19159541802440572 1102 50-51 0.22861270342318107 1102 52-53 0.2912615260397686 1102 54-55 0.22208879570041518 1102 56-57 0.29450762641430117 1102 58-59 0.2318030545812988 1102 60-61 0.18327893559578712 1102 62-63 0.2974933538632527 1102 64-65 0.2636379525778665 1102 66-67 0.3123888018137677 1102 68-69 0.38184221666097784 1102 70-71 0.424021614441628 1102 72-73 0.3011445651552549 1102 74-75 0.3623740631974428 1102 76-77 0.37914579190624664 1102 78-79 0.3485493479190751 1102 80-81 0.3315168851984609 1102 82-83 0.3030117399646777 1102 84-85 0.4102960034785177 1102 86-87 0.40007503134714284 1102 88-89 0.2377297246649377 1102 90-91 0.2327346442559879 1102 92-93 0.20916520941627326 1102 94-95 0.26669473560864176 1102 96-97 0.13734609805919717 1102 98-99 0.2872063726093863 1103 1 0.13492956489948682 1103 2 0.22234931922055878 1103 3 0.14408985048172696 1103 4 0.1355337369352796 1103 5 0.16911464663784415 1103 6 0.2691068339476317 1103 7 0.1598011899552958 1103 8 0.21833026945204637 1103 9 0.19345254097953557 1103 10-11 0.21045649340273087 1103 12-13 0.2909229855977884 1103 14-15 0.42722526315407805 1103 16-17 0.2367876127702857 1103 18-19 0.22286734103390415 1103 20-21 0.2641055511562129 1103 22-23 0.23674806961518868 1103 24-25 0.07567121174744074 1103 26-27 0.24520443553124238 1103 28-29 0.182428816047846 1103 30-31 0.39540523264491867 1103 32-33 0.45475109992381135 1103 34-35 0.5196243676431749 1103 36-37 0.6188651424097742 1103 38-39 0.5539099417125541 1103 40-41 0.3597477308700121 1103 42-43 0.2478705926908944 1103 44-45 0.19246129925175381 1103 46-47 0.17523097608543736 1103 48-49 0.17959470988147785 1103 50-51 0.12145099379296198 1103 52-53 0.17354190464818942 1103 54-55 0.09702613463139897 1103 56-57 0.15722326553961352 1103 58-59 0.11594565383276034 1103 60-61 -0.007617370925501632 1103 62-63 0.14466147284109354 1103 64-65 0.2710180206656432 1103 66-67 0.24210186845034798 1103 68-69 0.3557452065260023 1103 70-71 0.3589530429393335 1103 72-73 0.11178578233094072 1103 74-75 0.1805523902617523 1103 76-77 0.08100468285633866 1103 78-79 0.10069360044484554 1103 80-81 -0.013523459539570126 1103 82-83 0.036743496115466456 1103 84-85 0.13326856373712204 1103 86-87 0.07892792730586962 1103 88-89 0.1387691126828159 1103 90-91 0.04695828230655863 1103 92-93 0.023659410960636507 1103 94-95 0.14799167599854002 1103 96-97 -0.013426188150148732 1103 98-99 0.04427808921461107 1104 1 0.1318942350784269 1104 2 0.0876101684343098 1104 3 0.058365034261701254 1104 4 0.0654218176040473 1104 5 0.11966718899098794 1104 6 0.08454609551523617 1104 7 0.03690459759592102 1104 8 0.1219418239292267 1104 9 0.08804350363210034 1104 10-11 0.0847432104446213 1104 12-13 0.1337897110328612 1104 14-15 0.14963892205155815 1104 16-17 0.002945762129513696 1104 18-19 0.03816876394179758 1104 20-21 0.09685974835198152 1104 22-23 0.019763614668399043 1104 24-25 -0.012040244748213524 1104 26-27 0.06632760523244485 1104 28-29 0.01874401297315842 1104 30-31 0.2657105301843572 1104 32-33 0.3086685333642123 1104 34-35 0.3885317778220383 1104 36-37 0.46739698686199915 1104 38-39 0.4958476291824496 1104 40-41 0.25642317298929385 1104 42-43 0.16052360579114833 1104 44-45 0.07349777529888968 1104 46-47 0.10739579542559596 1104 48-49 -0.05470118789734357 1104 50-51 -0.09980317948493678 1104 52-53 0.01815494635306436 1104 54-55 -0.16864144455757213 1104 56-57 0.03630834828665286 1104 58-59 -0.06409991349647015 1104 60-61 -0.12969950375671147 1104 62-63 -0.07844748152339065 1104 64-65 0.0030607459221982936 1104 66-67 -0.033272698352952546 1104 68-69 0.016641613104617647 1104 70-71 -0.007831377036957576 1104 72-73 -0.142988902707188 1104 74-75 -0.15950876099134348 1104 76-77 -0.05056317263489163 1104 78-79 -0.07529215169534709 1104 80-81 -0.1316177353260386 1104 82-83 -0.08062054806279306 1104 84-85 -0.01660842276409724 1104 86-87 -0.13165271087035535 1104 88-89 -0.09220979030500587 1104 90-91 -0.1922732963267002 1104 92-93 -0.18125549470965296 1104 94-95 -0.15448060854141943 1104 96-97 -0.22677634372763578 1104 98-99 -0.18168207471744324 1105 1 0.1306615583345554 1105 2 0.13711203190884547 1105 3 0.11103625964764419 1105 4 -0.0010997628067670462 1105 5 0.04751479678213144 1105 6 0.1933753313337263 1105 7 0.15093926537274172 1105 8 0.17969973131314276 1105 9 0.151554264677209 1105 10-11 0.14337988282766645 1105 12-13 0.17432521202327678 1105 14-15 0.2556863833055374 1105 16-17 0.07781271855016314 1105 18-19 0.19031287681391262 1105 20-21 0.1684022572158952 1105 22-23 0.10987753800708333 1105 24-25 -0.0339174177863768 1105 26-27 0.1006107727145178 1105 28-29 0.08912061679383498 1105 30-31 0.2703956483337322 1105 32-33 0.38980398500667235 1105 34-35 0.3629091598839409 1105 36-37 0.439376334445825 1105 38-39 0.43667427603610776 1105 40-41 0.25788415405745724 1105 42-43 0.10280709670065136 1105 44-45 0.1133677002270872 1105 46-47 0.04762694994209937 1105 48-49 -0.1313699982983323 1105 50-51 -0.04192191830594538 1105 52-53 0.011771810012874084 1105 54-55 0.008194096293046016 1105 56-57 0.11665883001356292 1105 58-59 -0.04359222433593146 1105 60-61 -0.11469037882753952 1105 62-63 -0.004104557393588948 1105 64-65 0.04759857291156777 1105 66-67 0.04478049802348494 1105 68-69 0.09281935451031842 1105 70-71 0.09083913127759047 1105 72-73 -0.06973359830887915 1105 74-75 0.11281689959535157 1105 76-77 -0.002548339723347226 1105 78-79 -0.06735803012326613 1105 80-81 -0.07810486537049499 1105 82-83 0.059651908968056944 1105 84-85 0.04688049823645102 1105 86-87 -0.11970055633527465 1105 88-89 -0.09968355255197281 1105 90-91 -0.1011073283429269 1105 92-93 -0.15332394098272673 1105 94-95 -0.0571191225549299 1105 96-97 -0.14765041764454878 1105 98-99 -0.278375525775445 1106 1 0.1034758884829543 1106 2 0.14656655996967416 1106 3 0.03820770576486865 1106 4 -0.0023229172811767285 1106 5 0.04006964503852117 1106 6 0.08877397296315337 1106 7 0.08956083342400945 1106 8 0.07809191347591593 1106 9 0.10063495044560256 1106 10-11 0.08874390286319311 1106 12-13 0.09283705558709698 1106 14-15 0.210293943381906 1106 16-17 0.04367740300351386 1106 18-19 0.2052953390676322 1106 20-21 0.11177090008329316 1106 22-23 0.08455825457124178 1106 24-25 0.09689212861676566 1106 26-27 0.1306863074422182 1106 28-29 0.03581901223839168 1106 30-31 0.24281805352860175 1106 32-33 0.19416290947800974 1106 34-35 0.3955820273107804 1106 36-37 0.43953551418788095 1106 38-39 0.46200101806729066 1106 40-41 0.2732621937305595 1106 42-43 0.148664199627774 1106 44-45 0.07047469352112756 1106 46-47 0.05180922430067625 1106 48-49 -0.03101132378646554 1106 50-51 -0.06634236023145945 1106 52-53 -0.0024638628450190936 1106 54-55 -0.07567316687212866 1106 56-57 -0.07946840566394542 1106 58-59 -0.1039099281886493 1106 60-61 -0.11328908094526469 1106 62-63 0.046791191144031075 1106 64-65 0.08834000929755348 1106 66-67 0.04769539824812341 1106 68-69 0.06779348189208889 1106 70-71 0.08878175281191147 1106 72-73 -0.06905907021180724 1106 74-75 0.13197686081538507 1106 76-77 0.042913808319013924 1106 78-79 -0.012029634041201831 1106 80-81 0.023960160814965548 1106 82-83 0.046687408493394145 1106 84-85 0.12816385653814777 1106 86-87 0.036041230324943996 1106 88-89 -0.019444767966529497 1106 90-91 -0.08064468581894246 1106 92-93 -0.11511323003004748 1106 94-95 0.0719048211515485 1106 96-97 -0.07713384313271376 1106 98-99 -0.15745029273669786 1107 1 0.10562882040721533 1107 2 0.1589748282706438 1107 3 0.1057457363196086 1107 4 0.05326451460353354 1107 5 0.012205312556773151 1107 6 0.12338561689757199 1107 7 0.10690737332991773 1107 8 0.10719174455464042 1107 9 0.00850956711528994 1107 10-11 0.03587235748945261 1107 12-13 0.09509445810835615 1107 14-15 0.1799950271770996 1107 16-17 0.008027684306298966 1107 18-19 0.16345986746082986 1107 20-21 0.09428289442467275 1107 22-23 -0.01774963819399744 1107 24-25 0.005676733047813798 1107 26-27 0.10740728020540757 1107 28-29 0.09064525134343882 1107 30-31 0.2864577951674647 1107 32-33 0.32708359558691313 1107 34-35 0.33799325057073304 1107 36-37 0.43980436892023533 1107 38-39 0.45501800791804925 1107 40-41 0.2730811734088263 1107 42-43 0.14226147827342572 1107 44-45 0.172749895598443 1107 46-47 0.04958044067348055 1107 48-49 -0.051124356784285396 1107 50-51 -0.039599283444410105 1107 52-53 0.023230436178216962 1107 54-55 -0.042824726020164405 1107 56-57 0.027110449859506502 1107 58-59 -0.01983075855537919 1107 60-61 -0.07830376267058625 1107 62-63 -0.03587250660321928 1107 64-65 0.08211423647107807 1107 66-67 0.12489661001398389 1107 68-69 0.15993119694098112 1107 70-71 0.14688613499280478 1107 72-73 -0.20257714664984405 1107 74-75 0.09047376392530282 1107 76-77 0.00855577897417703 1107 78-79 0.0023912638009804255 1107 80-81 -0.010841461387713025 1107 82-83 0.004808892208547633 1107 84-85 0.10413678259902426 1107 86-87 -0.04689085680679028 1107 88-89 -0.18056425533931275 1107 90-91 -0.14683541795074717 1107 92-93 -0.05408506717690997 1107 94-95 0.037102500927062465 1107 96-97 -0.047566686454736384 1107 98-99 -0.09026988202066732 1108 1 0.06611160162764662 1108 2 0.003927293908564167 1108 3 -0.036035624024730595 1108 4 0.0033557356585660614 1108 5 -0.016669879366432383 1108 6 0.07337006053198536 1108 7 0.07056457362595125 1108 8 0.09223885548901478 1108 9 0.04671600100718365 1108 10-11 -0.04128689366945082 1108 12-13 -0.07183896801087997 1108 14-15 -0.03928184115161315 1108 16-17 -0.07486602061246117 1108 18-19 0.14920973030707785 1108 20-21 0.0501250000754041 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2215 88-89 0.07108669180179561 2215 90-91 0.17150718949100607 2215 92-93 0.04583996662330847 2215 94-95 0.26092922623761794 2215 96-97 0.3416043070510355 2215 98-99 0.4413632373083516 2216 1 -0.29379035469355586 2216 2 -0.2771604164833015 2216 3 -0.17158979149874298 2216 4 -0.20387596145504716 2216 5 -0.4034676297594757 2216 6 -0.28373967020357327 2216 7 -0.4285244310974008 2216 8 -0.45795061860000175 2216 9 -0.3211720715038666 2216 10-11 -0.3960355150971324 2216 12-13 -0.46620383538311927 2216 14-15 -0.4333543205099346 2216 16-17 -0.4566808043355124 2216 18-19 -0.390424326596289 2216 20-21 -0.21644280036253605 2216 22-23 -0.20554614392852244 2216 24-25 -0.2731307352542629 2216 26-27 -0.3722568369656045 2216 28-29 -0.35796983576189945 2216 30-31 -0.2385851198850304 2216 32-33 -0.1308097578002858 2216 34-35 -0.20679111621565482 2216 36-37 -0.03800408874665351 2216 38-39 -8.61541713072145 2216 40-41 -1.8871425846474637 2216 42-43 -0.5301217968795342 2216 44-45 -0.5483265217957722 2216 46-47 -0.22145487698468713 2216 48-49 -0.0863367543612199 2216 50-51 -0.16928977997476125 2216 52-53 -0.26629176906402563 2216 54-55 -0.21667334681701078 2216 56-57 -0.08292277980294926 2216 58-59 -0.1183257501563375 2216 60-61 -0.17959957927907055 2216 62-63 -0.19710882942604968 2216 64-65 -0.2380601803819431 2216 66-67 -0.15460065076722884 2216 68-69 -0.14113740313835166 2216 70-71 0.06953531755893749 2216 72-73 0.01780654529699177 2216 74-75 0.021027854732224682 2216 76-77 -0.05222374358996262 2216 78-79 -0.0885245942341939 2216 80-81 -0.036152994679639505 2216 82-83 -0.1574864994145635 2216 84-85 -0.07093075203186672 2216 86-87 -0.01755326403995383 2216 88-89 0.021076152063507436 2216 90-91 0.01924554710618054 2216 92-93 0.01720817466725677 2216 94-95 0.1781711336334979 2216 96-97 0.23721540476481096 2216 98-99 0.21750872462434856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 319.0 8 2142.0 9 4026.0 10 5399.0 11 7856.0 12 13223.0 13 21666.0 14 29379.0 15 32206.0 16 34471.0 17 37615.0 18 40643.0 19 44365.0 20 48885.0 21 54535.0 22 61381.0 23 68880.0 24 78349.0 25 87971.0 26 97259.0 27 109474.0 28 129166.0 29 160543.0 30 201735.0 31 258419.0 32 343902.0 33 461392.0 34 628756.0 35 899210.0 36 1352260.0 37 1586357.0 38 464930.0 39 378.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.31755687722815 19.296409331438056 12.66818024361295 24.71785354772084 2 15.926870834868906 20.776534162982994 38.030803729894195 25.265791272253907 3 17.813935992773906 26.692709305480918 29.00015298704144 26.49320171470374 4 12.893640466522974 17.642295194430044 36.814528164893176 32.64953617415381 5 14.127148832702519 37.73955519139302 33.44977665633093 14.683519319573529 6 31.131624844901594 37.4034575627279 17.64241735923933 13.822500233131176 7 27.303738839149368 32.17949229582658 22.339985388987664 18.17678347603639 8 26.79333528525551 33.62893145020352 19.939115773825158 19.638617490715816 9 26.687364100133337 15.673446406366034 19.64126439491702 37.99792509858361 10-11 25.263534962518815 26.059099402322204 26.681162653427982 21.996202981731 12-13 26.12061561900501 23.941084463619212 26.252289065599722 23.686010851776054 14-15 24.16890562806549 24.70338540736971 23.752949517455345 27.374759447109447 16-17 23.77329380979128 27.235551478562897 24.699467333488435 24.29168737815739 18-19 24.167660613128888 26.67294186570368 26.016360311259646 23.14303720990778 20-21 25.736684341199794 25.955427219161088 24.76664145999435 23.541246979644765 22-23 25.269609341918986 25.564040206081245 24.633619128952397 24.53273132304737 24-25 24.581610652811786 26.127674355675012 24.838593400883383 24.452121590629822 26-27 24.582481495995818 26.288880059628728 24.559513956920117 24.56912448745534 28-29 24.18792618091338 26.199448849738694 25.163023563394194 24.449601405953732 30-31 24.65859852016713 26.097254032998624 24.99658269345654 24.247564753377702 32-33 24.31145325977686 26.31269853637242 24.490080002302147 24.885768201548576 34-35 24.529024118998297 26.301262220383414 25.141083387391262 24.028630273227026 36-37 24.6272137302517 26.088835618948597 24.757597640316597 24.526353010483106 38-39 24.632667283941643 25.901080439477692 24.93359223206026 24.532660044520405 40-41 24.343857018564968 25.828506140032413 25.31760994187685 24.510026899525762 42-43 24.731631902410093 25.87110743499437 25.01143771675415 24.385822945841383 44-45 24.28204759622413 26.303366169876686 24.876759461698413 24.537826772200773 46-47 24.082353071326995 26.307433973950577 25.28065054118444 24.32956241353798 48-49 24.751219596286976 26.154439958955294 25.031588119025837 24.062752325731893 50-51 23.920110514051327 26.391711337558554 24.917093421810456 24.771084726579666 52-53 23.940233218936864 26.211964351087254 24.802869363650593 25.044933066325292 54-55 24.097592308069242 26.130842311428776 24.795879571016915 24.975685809485064 56-57 24.42136000268221 25.80662106819017 24.685719226627615 25.08629970250001 58-59 24.00172170949573 25.834981257541557 25.465039042178965 24.698257990783752 60-61 24.18663369296683 26.060774253474932 25.010826876062964 24.741765177495274 62-63 23.848815004180413 26.10258034596683 24.88494275252513 25.16366189732762 64-65 23.74721182021946 25.953179958701174 25.124705826460094 25.174902394619274 66-67 24.52972844349598 25.96249745571243 24.831459400636096 24.676314700155498 68-69 24.215672454259 25.614641681266125 25.062842783058574 25.106843081416297 70-71 23.716693700168186 25.888920779952095 24.9740823840501 25.42030313582962 72-73 24.519893877600886 25.995101103371603 24.976092597155002 24.50891242187251 74-75 24.799666456901377 25.832405316491297 24.89252017520379 24.475408051403534 76-77 24.242686259381586 26.07946256134714 25.03952034262637 24.638330836644904 78-79 24.467600128924726 26.032641520100984 24.960542423164533 24.539215927809753 80-81 24.11667037911948 25.955746205052005 25.284437004155098 24.643146411673417 82-83 24.41293020547977 25.841529654474154 25.198092513101027 24.547447626945047 84-85 24.57804540471365 26.04688210193097 24.65675393062451 24.71831856273087 86-87 24.277432481206638 25.687377266785006 25.05408168237774 24.981108569630617 88-89 24.53775211592843 25.10465112648849 25.21830512072801 25.13929163685506 90-91 24.668544486994254 25.429303335988397 25.04932094954671 24.852831227470634 92-93 24.496840478685158 25.40736873696654 25.017486535062588 25.07830424928571 94-95 23.854321451176766 25.60934110138634 25.5428427235314 24.993494723905496 96-97 24.530307686710888 25.465721820633096 25.048114226390567 24.955856266265446 98-99 24.39092527855822 25.719493492870477 24.706458872002525 25.183122356568777 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1058.0 1 980.0 2 889.5 3 858.0 4 805.0 5 724.0 6 683.0 7 710.0 8 694.0 9 640.5 10 624.0 11 594.0 12 598.5 13 648.0 14 679.0 15 855.5 16 1058.0 17 1273.5 18 1697.5 19 2264.5 20 3321.5 21 4876.5 22 6898.5 23 8698.0 24 10838.0 25 14828.5 26 19952.5 27 24329.0 28 29683.5 29 38698.0 30 48102.0 31 59868.5 32 72348.5 33 82869.5 34 91113.0 35 100454.5 36 109375.5 37 122099.0 38 140757.0 39 144358.0 40 147320.0 41 157666.0 42 172165.0 43 194977.0 44 218786.0 45 251466.0 46 288992.0 47 319264.5 48 336500.5 49 349163.5 50 369889.5 51 365498.0 52 349223.0 53 346875.5 54 327720.0 55 307533.5 56 275231.5 57 242925.5 58 231101.5 59 203369.0 60 169791.5 61 134294.0 62 105749.5 63 83787.5 64 60344.5 65 43995.0 66 31052.0 67 23125.5 68 18389.0 69 15871.5 70 14043.0 71 12129.0 72 11518.0 73 10202.5 74 7434.0 75 5551.5 76 4112.5 77 2780.5 78 1804.5 79 1209.0 80 845.0 81 577.5 82 411.0 83 302.0 84 218.5 85 170.0 86 116.5 87 72.5 88 54.5 89 50.0 90 37.0 91 20.0 92 16.0 93 17.0 94 16.0 95 16.0 96 9.5 97 4.0 98 5.5 99 7.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.3573867698497794E-5 2 0.0017917505362017086 3 0.006243979141308985 4 0.0 5 0.0057417460364645664 6 0.0 7 6.922672526233875E-4 8 0.0 9 0.0 10-11 6.786933849248897E-6 12-13 1.0859094158798235E-4 14-15 0.002904807687478528 16-17 1.4931254468347573E-4 18-19 0.0 20-21 0.0 22-23 3.3934669246244486E-5 24-25 8.890883342516055E-4 26-27 0.002416148450332607 28-29 0.0019071284116389399 30-31 0.002497591656523594 32-33 8.008581942113697E-4 34-35 0.0 36-37 9.501707388948457E-5 38-39 0.06284700744404478 40-41 0.003237367446091724 42-43 3.1898589091469817E-4 44-45 0.0 46-47 8.347928634576144E-4 48-49 1.0180400773873344E-4 50-51 9.501707388948456E-4 52-53 3.8006829555793827E-4 54-55 0.0 56-57 5.158069725429162E-4 58-59 6.786933849248897E-6 60-61 1.832472139297202E-4 62-63 4.750853694474228E-5 64-65 8.076451280606187E-4 66-67 0.0014048953067945216 68-69 0.001377747571397526 70-71 4.750853694474228E-5 72-73 0.0017917505362017086 74-75 0.0018053244039002065 76-77 6.786933849248897E-5 78-79 1.0180400773873344E-4 80-81 0.0 82-83 2.9183815551770255E-4 84-85 5.768893771861562E-4 86-87 0.0 88-89 0.0 90-91 6.108240464324007E-4 92-93 0.0 94-95 0.0 96-97 0.0034341885277199414 98-99 0.005015544114594935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7367097.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.726209944430494 #Duplication Level Percentage of deduplicated Percentage of total 1 73.51630390269499 24.059099958581513 2 13.15871249618982 8.612695754974183 3 4.875081475435309 4.7862881958389965 4 2.3695005675517318 3.1017909214858075 5 1.3441139800005169 2.199387814937048 6 0.8498978924161188 1.668836211512334 7 0.6000472044967193 1.3746089553649778 8 0.43042644545143294 1.1268980975582843 9 0.32546774443669785 0.9586193161118062 >10 2.0477280121424823 12.868000447600492 >50 0.21808640863194423 5.013087948064952 >100 0.21035606406130375 14.575672138612585 >500 0.03456709276072913 7.770490366024436 >1k 0.01937592453741783 10.884178859397448 >5k 2.510918946464219E-4 0.5333155607411472 >10k+ 8.369729821547396E-5 0.46702945319416195 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 17581 0.2386421680072897 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 16520 0.22424029437918355 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 7947 0.10787152659996198 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.3573867698497794E-5 0.0 4 0.0 0.0 0.0 4.072160309549338E-5 0.0 5 0.0 0.0 0.0 9.501707388948455E-5 0.0 6 5.4295470793991175E-5 0.0 0.0 9.501707388948455E-5 0.0 7 5.4295470793991175E-5 0.0 0.0 9.501707388948455E-5 0.0 8 5.4295470793991175E-5 0.0 0.0 1.0859094158798235E-4 0.0 9 5.4295470793991175E-5 0.0 0.0 1.2216480928648013E-4 1.3573867698497794E-5 10-11 7.465627234173786E-5 0.0 0.0 1.4252561083422685E-4 1.3573867698497794E-5 12-13 8.144320619098676E-5 0.0 0.0 1.6967334623122242E-4 1.3573867698497794E-5 14-15 8.823014004023566E-5 0.0 0.0 2.0360801547746692E-4 2.7147735396995587E-5 16-17 1.0859094158798235E-4 6.786933849248897E-6 0.0 2.0360801547746692E-4 4.072160309549338E-5 18-19 1.6967334623122242E-4 1.3573867698497794E-5 0.0 2.171818831759647E-4 4.072160309549338E-5 20-21 1.900341477789691E-4 1.3573867698497794E-5 0.0 3.25772824763947E-4 4.072160309549338E-5 22-23 2.103949493267158E-4 1.3573867698497794E-5 0.0 0.0010926963497290725 4.7508536944742276E-5 24-25 2.239688170252136E-4 1.3573867698497794E-5 0.0 0.002945529290574021 5.4295470793991175E-5 26-27 2.714773539699559E-4 1.3573867698497794E-5 0.0 0.0047237059590772325 5.4295470793991175E-5 28-29 2.7826428781920474E-4 1.3573867698497794E-5 0.0 0.012806944173532668 5.4295470793991175E-5 30-31 3.1898589091469817E-4 1.3573867698497794E-5 0.0 0.03925562538405562 6.108240464324007E-5 32-33 3.4613362631169374E-4 1.3573867698497794E-5 0.0 0.09063271462286976 9.501707388948455E-5 34-35 3.5292056016094264E-4 1.3573867698497794E-5 0.0 0.16087747996259585 1.0859094158798235E-4 36-37 3.6649442785944045E-4 1.3573867698497794E-5 0.0 0.27261077192278044 1.1537787543723124E-4 38-39 3.800682955579382E-4 1.3573867698497794E-5 0.0 0.44106925699498734 1.2216480928648013E-4 40-41 3.800682955579382E-4 1.3573867698497794E-5 0.0 0.6392816600623012 1.2216480928648013E-4 42-43 4.343637663519294E-4 1.3573867698497794E-5 0.0 0.823574061804806 1.2216480928648013E-4 44-45 4.750853694474228E-4 1.3573867698497794E-5 0.0 1.0452013866520287 1.2216480928648013E-4 46-47 4.750853694474228E-4 1.3573867698497794E-5 0.0 1.2888183771708177 1.2216480928648013E-4 48-49 4.818723032966717E-4 1.3573867698497794E-5 0.0 1.5490633556202669 1.2216480928648013E-4 50-51 4.886592371459205E-4 1.3573867698497794E-5 0.0 1.830965439982669 1.2216480928648013E-4 52-53 4.886592371459205E-4 1.3573867698497794E-5 0.0 2.106270624643601 1.2895174313572904E-4 54-55 4.886592371459205E-4 1.3573867698497794E-5 0.0 2.384792815949077 1.3573867698497794E-4 56-57 4.886592371459205E-4 1.3573867698497794E-5 0.0 2.7017765613782467 1.3573867698497794E-4 58-59 4.954461709951695E-4 1.3573867698497794E-5 0.0 3.0498987593077707 1.3573867698497794E-4 60-61 5.022331048444183E-4 1.3573867698497794E-5 0.0 3.396500684055063 1.3573867698497794E-4 62-63 5.022331048444183E-4 1.3573867698497794E-5 0.0 3.7493805226129098 1.4931254468347573E-4 64-65 5.022331048444183E-4 1.3573867698497794E-5 0.0 4.1215420402364735 1.4931254468347573E-4 66-67 5.022331048444183E-4 1.3573867698497794E-5 0.0 4.527672161775527 1.4931254468347573E-4 68-69 5.022331048444183E-4 1.3573867698497794E-5 0.0 4.965836611083036 1.4931254468347573E-4 70-71 5.090200386936672E-4 1.3573867698497794E-5 0.0 5.444688728816792 1.4931254468347573E-4 72-73 5.29380840241414E-4 1.3573867698497794E-5 0.0 5.91357762765985 1.4931254468347573E-4 74-75 5.29380840241414E-4 1.3573867698497794E-5 0.0 6.383742470066568 1.4931254468347573E-4 76-77 5.361677740906628E-4 1.3573867698497794E-5 0.0 6.895022557732035 1.560994785327246E-4 78-79 5.429547079399118E-4 2.036080154774669E-5 0.0 7.416157273346611 1.628864123819735E-4 80-81 5.429547079399118E-4 4.072160309549338E-5 0.0 7.981841422747658 1.6967334623122242E-4 82-83 5.429547079399118E-4 4.072160309549338E-5 0.0 8.575603117483046 1.7646028008047132E-4 84-85 5.429547079399118E-4 4.072160309549338E-5 0.0 9.164953305216423 1.7646028008047132E-4 86-87 5.429547079399118E-4 4.072160309549338E-5 0.0 9.76939084689668 1.7646028008047132E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 3210 0.0 26.798363 1 GTATAAG 3840 0.0 22.88612 1 GTGATCG 2650 0.0 22.808556 8 TAGTACT 4040 0.0 22.442804 4 TAGGCAT 4935 0.0 20.916533 5 TATTCGC 685 0.0 20.363186 9 GTACATA 1630 0.0 19.968801 1 TGATCGC 2965 0.0 19.91563 9 CTAGGCA 5330 0.0 19.802614 4 CGTTAAC 920 0.0 19.71144 1 CTAGTAC 4795 0.0 19.685616 3 GTATCAA 35935 0.0 19.435417 1 GTACAAG 3245 0.0 19.201345 1 ATAAGGT 3360 0.0 18.820936 3 TACGGGC 2745 0.0 18.632631 4 CTGTGCG 4915 0.0 18.447014 9 ATACGGG 2780 0.0 18.3988 3 GTACAAA 3335 0.0 18.125463 1 GGCGTCG 2960 0.0 18.063688 8 GTACTGT 5355 0.0 18.059559 6 >>END_MODULE