FastQCFastQC Report
Fri 27 May 2016
ERR765403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7367097
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195510.2653826873733304No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT186240.25279971201682294No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA166260.22567912435522433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123990.16830238559367414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98150.13322751146075584No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC80230.1089031405450478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA453750.027.7800241
TATCACG4950.023.486992
CAACGCA546400.022.7829325
ATCAACG549800.022.7266223
AACGCAG580350.021.922016
TATCAAC579850.021.7824482
AGTACTT287400.021.73963712-13
TAGGCAT48550.021.4551095
TCAACGC587200.021.342474
CGCAGAG611850.020.0719478
TAGTACT42650.019.9528064
CTAGGCA53700.019.9170724
ACGCAGA617150.019.9065657
GAGTACT269650.019.4195612-13
TACTTTT328750.019.18887714-15
GCAGAGT660950.018.5390249
GGTATCA319600.018.519131
CTAGTAC50150.017.9888213
CGTTAAC13550.017.8568291
CTGTGCG50650.017.627749
Produced by FastQC (version 0.11.5)