##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765403_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7367097 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30185987777818 33.0 33.0 33.0 33.0 33.0 2 32.15332538719118 33.0 33.0 33.0 33.0 33.0 3 32.206089047015396 33.0 33.0 33.0 33.0 33.0 4 36.14778114092973 37.0 37.0 37.0 33.0 37.0 5 36.07568001344356 37.0 37.0 37.0 33.0 37.0 6 36.07421539311889 37.0 37.0 37.0 33.0 37.0 7 35.997405762405464 37.0 37.0 37.0 33.0 37.0 8 35.95882530120073 37.0 37.0 37.0 33.0 37.0 9 35.701198178875615 37.0 37.0 37.0 33.0 37.0 10-11 35.877682688309925 37.0 37.0 37.0 33.0 37.0 12-13 35.80487280132188 37.0 37.0 37.0 33.0 37.0 14-15 37.192059776055615 37.0 37.0 40.0 33.0 40.0 16-17 37.336932105006895 37.0 37.0 40.0 33.0 40.0 18-19 37.40374539659244 37.0 37.0 40.0 33.0 40.0 20-21 37.429803218282586 37.0 37.0 40.0 33.0 40.0 22-23 37.35418455872103 37.0 37.0 40.0 33.0 40.0 24-25 37.29838985423973 37.0 37.0 40.0 33.0 40.0 26-27 37.169130866608654 37.0 37.0 40.0 33.0 40.0 28-29 37.114064943084095 37.0 37.0 40.0 33.0 40.0 30-31 37.02049063559228 37.0 37.0 40.0 33.0 40.0 32-33 36.85727919694827 37.0 37.0 40.0 33.0 40.0 34-35 36.77443700008294 37.0 37.0 40.0 33.0 40.0 36-37 36.63739658918567 37.0 37.0 40.0 33.0 40.0 38-39 36.497506005418415 37.0 37.0 40.0 33.0 40.0 40-41 36.36899677036966 37.0 37.0 40.0 33.0 40.0 42-43 36.20116505863843 37.0 37.0 40.0 33.0 40.0 44-45 35.997969213653626 37.0 37.0 40.0 33.0 40.0 46-47 35.758713371087694 37.0 37.0 40.0 27.0 40.0 48-49 35.5111068172443 37.0 37.0 40.0 27.0 40.0 50-51 35.21605525215699 37.0 33.0 40.0 27.0 40.0 52-53 35.05792308693641 37.0 33.0 40.0 27.0 40.0 54-55 34.93459682966031 37.0 33.0 40.0 27.0 40.0 56-57 34.74361393639856 37.0 33.0 40.0 27.0 40.0 58-59 34.537601445996984 37.0 33.0 38.5 27.0 40.0 60-61 34.358678391230626 37.0 33.0 37.0 27.0 40.0 62-63 34.037751233627034 37.0 33.0 37.0 27.0 40.0 64-65 33.884035665608856 37.0 33.0 37.0 27.0 40.0 66-67 33.688706148432686 37.0 33.0 37.0 27.0 40.0 68-69 33.44578787275368 37.0 33.0 37.0 27.0 40.0 70-71 33.26082071404788 37.0 33.0 37.0 27.0 37.0 72-73 33.06436755210363 37.0 33.0 37.0 27.0 37.0 74-75 32.8634165533588 37.0 33.0 37.0 27.0 37.0 76-77 32.70631953128892 35.0 33.0 37.0 27.0 37.0 78-79 32.51873105512253 33.0 33.0 37.0 22.0 37.0 80-81 32.392002575777134 33.0 33.0 37.0 22.0 37.0 82-83 32.25110935827233 33.0 33.0 37.0 22.0 37.0 84-85 32.09887700677757 33.0 33.0 37.0 22.0 37.0 86-87 32.00730172278171 33.0 33.0 37.0 22.0 37.0 88-89 31.91431054864623 33.0 33.0 37.0 22.0 37.0 90-91 31.850131333956917 33.0 33.0 37.0 22.0 37.0 92-93 31.7534698538651 33.0 33.0 37.0 22.0 37.0 94-95 31.689197047358 33.0 33.0 37.0 22.0 37.0 96-97 31.643559464467483 33.0 33.0 37.0 22.0 37.0 98-99 30.810907132076583 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1767567947953026 1101 2 0.31743263326036697 1101 3 0.2401544843381771 1101 4 0.21051008585366304 1101 5 0.11868709877547445 1101 6 0.19022496880003104 1101 7 0.23033233339205594 1101 8 0.17660354337188977 1101 9 0.39000398879802134 1101 10-11 0.331269866776978 1101 12-13 0.331002577170878 1101 14-15 0.5063328557927207 1101 16-17 0.4232868166604362 1101 18-19 0.3090422234520247 1101 20-21 0.3552070885643346 1101 22-23 0.2865473348756993 1101 24-25 0.3540458335224841 1101 26-27 0.2760064238580995 1101 28-29 0.2277047074144818 1101 30-31 0.21571177750795556 1101 32-33 0.35753904659308233 1101 34-35 0.40618603136297793 1101 36-37 0.4381842059110781 1101 38-39 0.1602820473020259 1101 40-41 0.16139312768327585 1101 42-43 0.3378189689684845 1101 44-45 0.37399211145940825 1101 46-47 0.4306820205375814 1101 48-49 0.45941959647830544 1101 50-51 0.4423867225114648 1101 52-53 0.4220424600706636 1101 54-55 0.4242025345144782 1101 56-57 0.5392609029724653 1101 58-59 0.5192247621321116 1101 60-61 0.3252628628213259 1101 62-63 0.419153325543661 1101 64-65 0.36368110031364154 1101 66-67 0.36741904665444736 1101 68-69 0.26006170115542204 1101 70-71 0.2676144302315109 1101 72-73 0.3894200472647569 1101 74-75 0.21378073631229455 1101 76-77 0.25289485797259204 1101 78-79 0.4767363523674035 1101 80-81 0.4964120026441776 1101 82-83 0.4481626618214207 1101 84-85 0.45274645111020106 1101 86-87 0.2615717195744125 1101 88-89 0.3803617438071143 1101 90-91 0.3452130658083199 1101 92-93 0.47574727221706326 1101 94-95 0.48071005191336624 1101 96-97 0.3809714276387304 1101 98-99 0.45702651585109777 1102 1 0.1014532762803384 1102 2 0.34380566805965884 1102 3 0.24577957063933553 1102 4 0.17806328202212995 1102 5 0.10475177719668949 1102 6 0.23010998300006946 1102 7 0.14767611429720517 1102 8 0.11112016390909218 1102 9 0.410673791428934 1102 10-11 0.33217159311340083 1102 12-13 0.290544476617427 1102 14-15 0.527038744970568 1102 16-17 0.3717295080584151 1102 18-19 0.2858990054103785 1102 20-21 0.42525115946803993 1102 22-23 0.23972268693778886 1102 24-25 0.4027021736055687 1102 26-27 0.2750850506793512 1102 28-29 0.2664505671246431 1102 30-31 0.2902177475156762 1102 32-33 0.327112543760002 1102 34-35 0.34490027452646643 1102 36-37 0.4065425227638926 1102 38-39 0.23271345320723213 1102 40-41 0.30021801126667214 1102 42-43 0.3215959502432 1102 44-45 0.3349109892351052 1102 46-47 0.42751386829140614 1102 48-49 0.5199188923408045 1102 50-51 0.42104649064990696 1102 52-53 0.44254942340806025 1102 54-55 0.46521502422498173 1102 56-57 0.5467531140428363 1102 58-59 0.5022507317173179 1102 60-61 0.35827866286440013 1102 62-63 0.4947788115291445 1102 64-65 0.3871844507645861 1102 66-67 0.41164906603655993 1102 68-69 0.39081171171815754 1102 70-71 0.37103558315902774 1102 72-73 0.46227767344171156 1102 74-75 0.3573342279039693 1102 76-77 0.31990463506102174 1102 78-79 0.5954013099045667 1102 80-81 0.5426675544078137 1102 82-83 0.4447117441568551 1102 84-85 0.4556485108889774 1102 86-87 0.3422834885602768 1102 88-89 0.40426633634168496 1102 90-91 0.38191723666009025 1102 92-93 0.44148948102161967 1102 94-95 0.4116139026244845 1102 96-97 0.40464062043032456 1102 98-99 0.38362005677904065 1103 1 0.07638832568423481 1103 2 0.2529812076952993 1103 3 0.28065952794488425 1103 4 0.21255509890531954 1103 5 0.09069748445709536 1103 6 0.18158519968288545 1103 7 0.1642608325523014 1103 8 0.10867452062760208 1103 9 0.44641858895180775 1103 10-11 0.3023802633349817 1103 12-13 0.28342535484747344 1103 14-15 0.4470619402541587 1103 16-17 0.37503250784196496 1103 18-19 0.2262572938196925 1103 20-21 0.3080004221000294 1103 22-23 0.1833328260329523 1103 24-25 0.24953311447718107 1103 26-27 0.12117288311466012 1103 28-29 0.09007814298582417 1103 30-31 0.15044762682704516 1103 32-33 0.12288439074209379 1103 34-35 0.25487387788962224 1103 36-37 0.2334712222914206 1103 38-39 0.0744069916554082 1103 40-41 0.06941449218882667 1103 42-43 0.06647090445888182 1103 44-45 0.1601918145088419 1103 46-47 0.2751800880824433 1103 48-49 0.2477948924171116 1103 50-51 0.182215248736199 1103 52-53 0.16336190451510646 1103 54-55 0.1437086644948522 1103 56-57 0.27454379644461113 1103 58-59 0.21585871111934551 1103 60-61 0.10641642976925425 1103 62-63 0.1928352280001988 1103 64-65 -0.013655583198776355 1103 66-67 0.04484226697456961 1103 68-69 0.04679810947003915 1103 70-71 0.022042446277971806 1103 72-73 0.10010903454697484 1103 74-75 0.04725097296216774 1103 76-77 0.04751933185914936 1103 78-79 0.22297625377171926 1103 80-81 0.12092240729623427 1103 82-83 0.1524171932263414 1103 84-85 0.24497242947884246 1103 86-87 0.1786503608864578 1103 88-89 0.14656265464100926 1103 90-91 -0.0708710503716965 1103 92-93 0.005907894902669852 1103 94-95 0.19413094013449594 1103 96-97 0.11354961113091022 1103 98-99 0.08389984645268811 1104 1 0.08456560764819443 1104 2 0.2652326114067094 1104 3 0.2381814173882475 1104 4 0.14502359519294572 1104 5 0.04668555378531636 1104 6 0.16130926453457306 1104 7 0.15841950319703813 1104 8 0.04233120563000625 1104 9 0.3795752724163961 1104 10-11 0.25141724299915325 1104 12-13 0.2624881605898324 1104 14-15 0.42165123487163214 1104 16-17 0.2862834454982206 1104 18-19 0.186216868671643 1104 20-21 0.28890784955472526 1104 22-23 0.20091859650909782 1104 24-25 0.2990251441290681 1104 26-27 0.1836132151956491 1104 28-29 0.09179966612894219 1104 30-31 0.16282864844848888 1104 32-33 0.2081079211877892 1104 34-35 0.2597010448481569 1104 36-37 0.15012961367606437 1104 38-39 0.014164609926396565 1104 40-41 -0.02491557577002368 1104 42-43 0.11408143763265599 1104 44-45 0.104180771252679 1104 46-47 0.20271480942079023 1104 48-49 0.26292643782709746 1104 50-51 0.18751885108343203 1104 52-53 0.14336975291800513 1104 54-55 0.10806997228410609 1104 56-57 0.16951324895675413 1104 58-59 0.21881200522037147 1104 60-61 0.1798739242889198 1104 62-63 0.15592932153960248 1104 64-65 0.05052425951643613 1104 66-67 0.08471300112530855 1104 68-69 0.07892807119554135 1104 70-71 0.18071973305168143 1104 72-73 0.15162255063736296 1104 74-75 0.08905019683178494 1104 76-77 0.03009099896436851 1104 78-79 0.18977283391367905 1104 80-81 0.16728301842398707 1104 82-83 0.06525793389413792 1104 84-85 0.10642250722995783 1104 86-87 0.10075802957477009 1104 88-89 0.04957439704534394 1104 90-91 0.0752896501192879 1104 92-93 0.004500567994682569 1104 94-95 0.07335236462861516 1104 96-97 0.11874881404702364 1104 98-99 0.053024559266319216 1105 1 0.13821210551596153 1105 2 0.27857598120703386 1105 3 0.22455817951001222 1105 4 0.20053516928170012 1105 5 -0.04331942286341928 1105 6 0.12433202875870819 1105 7 0.10990453447236348 1105 8 -0.015362481479158419 1105 9 0.2919187746333094 1105 10-11 0.2483162061801636 1105 12-13 0.2475411512102781 1105 14-15 0.4127629927016798 1105 16-17 0.3362696331892252 1105 18-19 0.17137471323736264 1105 20-21 0.27153362124837344 1105 22-23 0.15457727763665474 1105 24-25 0.26553359475743576 1105 26-27 0.13628733166100915 1105 28-29 0.08065237780207468 1105 30-31 0.15000457832571357 1105 32-33 0.14541151974783162 1105 34-35 0.19785583771535187 1105 36-37 0.16728251584815013 1105 38-39 0.13544384222439732 1105 40-41 0.07857482151612061 1105 42-43 0.17275772553046664 1105 44-45 0.11342499367352588 1105 46-47 0.3017291767011372 1105 48-49 0.3034168743585113 1105 50-51 0.19783667246642977 1105 52-53 0.17755960421188632 1105 54-55 0.23584278868771946 1105 56-57 0.30446834933434985 1105 58-59 0.26340959755003723 1105 60-61 0.1481644573592149 1105 62-63 0.16608389593392303 1105 64-65 0.031968495665083196 1105 66-67 0.19134708509081122 1105 68-69 0.10196375255647183 1105 70-71 0.1646239381331327 1105 72-73 0.24652977016813082 1105 74-75 0.12739421213480284 1105 76-77 0.12906567829198679 1105 78-79 0.28241503518025723 1105 80-81 0.18846232416764508 1105 82-83 0.06158215418884794 1105 84-85 0.12745013191234023 1105 86-87 0.16727746099281404 1105 88-89 0.17188743572015852 1105 90-91 0.12686684903871637 1105 92-93 0.06004833691682521 1105 94-95 0.25113134550251814 1105 96-97 0.1344814734540769 1105 98-99 0.062472577835418264 1106 1 0.08444780800334684 1106 2 0.2436762876910734 1106 3 0.16631761368748954 1106 4 0.16989362054791712 1106 5 -0.02051742914503052 1106 6 0.11179209296488324 1106 7 0.09151268642944643 1106 8 0.04529187176763827 1106 9 0.3995263448751132 1106 10-11 0.17823057535912312 1106 12-13 0.16420620243896877 1106 14-15 0.3251889849568812 1106 16-17 0.22721757376978502 1106 18-19 0.12805720883213922 1106 20-21 0.23190370863253662 1106 22-23 0.0995924221706801 1106 24-25 0.20588771215817303 1106 26-27 0.037593452665824145 1106 28-29 0.12825148236085937 1106 30-31 0.15169436190981855 1106 32-33 0.12161721764185529 1106 34-35 0.22528641190426413 1106 36-37 0.13005732056336683 1106 38-39 0.09175447975739104 1106 40-41 0.11711549552570233 1106 42-43 0.12566479285096932 1106 44-45 0.15016261825631005 1106 46-47 0.2571582815507085 1106 48-49 0.3740642638856073 1106 50-51 0.17743155135467958 1106 52-53 0.16166538088412352 1106 54-55 0.12893926571693726 1106 56-57 0.20120482183403965 1106 58-59 0.2571081483130442 1106 60-61 0.2577044893701057 1106 62-63 0.25619401915874107 1106 64-65 0.06706655314425802 1106 66-67 0.12306115461784373 1106 68-69 0.09885981850795389 1106 70-71 0.14507526707515694 1106 72-73 0.25591319925753453 1106 74-75 0.1315710412853761 1106 76-77 0.17216094177268104 1106 78-79 0.23380021020350483 1106 80-81 0.20890334740339256 1106 82-83 0.14458332195349755 1106 84-85 0.0621228012313253 1106 86-87 0.0831899072489719 1106 88-89 0.011783860141267866 1106 90-91 -0.0251351159642752 1106 92-93 -0.03167436771720489 1106 94-95 0.18992351956446285 1106 96-97 0.16743848854077115 1106 98-99 0.0737292018206368 1107 1 0.10557972016428607 1107 2 0.24181070807703264 1107 3 0.1616741399587056 1107 4 0.13915925507300386 1107 5 0.0028204645974341247 1107 6 0.1001914358936915 1107 7 0.08424837349230785 1107 8 -0.040245581755741 1107 9 0.30490669695731043 1107 10-11 0.21095011485087412 1107 12-13 0.16199614906356885 1107 14-15 0.26543750398727894 1107 16-17 0.19220491630770198 1107 18-19 0.11796020706847088 1107 20-21 0.22673834726665376 1107 22-23 0.11041559928673195 1107 24-25 0.1877308852722308 1107 26-27 0.026806456346726293 1107 28-29 0.04709737310648876 1107 30-31 0.009363876505453561 1107 32-33 -0.05115876854662105 1107 34-35 0.11428686794518228 1107 36-37 0.11852728003156443 1107 38-39 0.03172309204350654 1107 40-41 -0.023136048265861575 1107 42-43 0.043766708688153244 1107 44-45 -0.007394514828533261 1107 46-47 0.053939237654439864 1107 48-49 0.2164539648065258 1107 50-51 0.14837057682317578 1107 52-53 0.08897222661665438 1107 54-55 0.05337514776056196 1107 56-57 0.17582032815578685 1107 58-59 0.13798665529093768 1107 60-61 0.13034936379597895 1107 62-63 0.17827233255191288 1107 64-65 0.009573754402566692 1107 66-67 -0.01875733179166872 1107 68-69 -0.02322768550567389 1107 70-71 0.05821564391386147 1107 72-73 0.163989427892254 1107 74-75 0.052758678853841445 1107 76-77 0.09053322472279746 1107 78-79 0.11987153407166318 1107 80-81 0.06343792973041218 1107 82-83 0.047027445307911364 1107 84-85 -0.005189186595174533 1107 86-87 0.06979975494061108 1107 88-89 -0.042211616677427344 1107 90-91 -0.032431421567530094 1107 92-93 -0.07909889992625807 1107 94-95 0.17537988622135714 1107 96-97 0.027107021252398056 1107 98-99 0.001642395828820753 1108 1 0.06706169871306855 1108 2 0.2062070658624009 1108 3 0.07579563793545674 1108 4 0.06352761140030339 1108 5 -0.11028232155684492 1108 6 0.055086812431376586 1108 7 0.04097203720971265 1108 8 -0.05631061288688244 1108 9 0.24941714089364098 1108 10-11 0.1655334435817295 1108 12-13 0.10283358780462493 1108 14-15 0.16719868708109686 1108 16-17 0.12938205268456215 1108 18-19 -0.04606422470995852 1108 20-21 0.20226869046400964 1108 22-23 0.01967886269925856 1108 24-25 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-0.41111014633939647 2215 92-93 -0.27249862517270884 2215 94-95 -0.2720667935360943 2215 96-97 -0.20399189590576228 2215 98-99 -0.13965108647833446 2216 1 -0.20154928781492032 2216 2 0.027090997794594784 2216 3 -0.11456152129755282 2216 4 -0.2788102812097861 2216 5 -0.23230922674601118 2216 6 -0.20160609530372398 2216 7 -0.23294097935397673 2216 8 -0.21702683064069106 2216 9 0.10582515683034899 2216 10-11 -0.1827399493356694 2216 12-13 -0.1138235201087241 2216 14-15 -0.44195911217957473 2216 16-17 -0.6156488541566105 2216 18-19 -0.5750391726000643 2216 20-21 -0.7609188823924669 2216 22-23 -0.6920146165478016 2216 24-25 -0.5388587002122378 2216 26-27 -0.6549119673171688 2216 28-29 -0.6997396236950806 2216 30-31 -0.37884453945428476 2216 32-33 -0.44374970895828625 2216 34-35 -0.48602449526930513 2216 36-37 -0.44969121839698545 2216 38-39 -0.49562077651174974 2216 40-41 -0.4467193170044723 2216 42-43 -0.5009878147124951 2216 44-45 -0.362720726151359 2216 46-47 -0.6411760338981551 2216 48-49 -0.8866879071366753 2216 50-51 -0.501456416231143 2216 52-53 -0.41118430711330944 2216 54-55 -0.41943032903872535 2216 56-57 -0.4783640699526117 2216 58-59 -0.3749165827198908 2216 60-61 -0.37151932572326274 2216 62-63 -0.40754982738724976 2216 64-65 -0.4980563989267637 2216 66-67 -0.44143295272360206 2216 68-69 -0.35932966449909287 2216 70-71 -0.41197641934216733 2216 72-73 -0.2959145810900665 2216 74-75 -0.31987216311561184 2216 76-77 -0.2958740918840732 2216 78-79 -0.20872666607853319 2216 80-81 -0.17016785379139776 2216 82-83 -0.12969022585004808 2216 84-85 -0.18746701581270742 2216 86-87 -0.3195889950401245 2216 88-89 -0.18863465885344155 2216 90-91 -0.19085696464564705 2216 92-93 -0.15058023701545764 2216 94-95 -0.22897946604597053 2216 96-97 -0.2953568289594912 2216 98-99 -0.22998378338060732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 44.0 10 96.0 11 190.0 12 320.0 13 535.0 14 1464.0 15 3560.0 16 7348.0 17 13026.0 18 20962.0 19 29774.0 20 39717.0 21 51179.0 22 62544.0 23 63008.0 24 56782.0 25 57571.0 26 68555.0 27 87671.0 28 115074.0 29 151138.0 30 198040.0 31 259861.0 32 341954.0 33 449073.0 34 594548.0 35 832441.0 36 1345149.0 37 1899117.0 38 615661.0 39 687.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11407244833656 19.368956300658425 13.161560985679827 25.35541026532519 2 15.825739197956135 20.99744380928075 37.462298562533 25.71451843023011 3 17.83399892793593 26.11646079860222 29.78352260055759 26.26601767290427 4 13.225874452311407 17.500855493011695 36.70303241561771 32.57023763905919 5 13.906528121637429 37.2434129054616 33.645556915534705 15.204502057366264 6 31.27587433693353 37.29854513928621 17.24410578549461 14.181474738285651 7 26.737234490057617 32.0451325671428 22.741617220460107 18.47601572233948 8 27.163005998156397 32.436874388921446 20.102436549973486 20.297683062948675 9 26.98907757254232 13.736225474152915 20.079820610636766 39.194876342668 10-11 25.43015247389847 25.09924872714449 27.173315350673406 22.29728344828363 12-13 26.65940193267443 22.956179347170263 27.04298585996628 23.341432860189027 14-15 24.205966730775508 23.539770501792578 25.355432818844996 26.89882994858691 16-17 23.194766225484923 26.228736009382676 26.171411002179724 24.40508676295268 18-19 23.49463431796812 25.000607430579507 28.732945962296952 22.771812289155417 20-21 24.727772689839703 24.418044176695382 27.834471298531838 23.019711834933084 22-23 24.23109129688397 23.98359218020341 27.732409387306834 24.05290713560579 24-25 23.842091396380418 24.579559628439803 27.885909470175296 23.69243950500448 26-27 23.91699199834073 24.716560675120743 27.524742785387513 23.841704541151014 28-29 23.44178446408402 24.55611077199065 28.20491572189154 23.79718904203379 30-31 23.984888484568618 24.432785397015948 27.93185701233471 23.650469106080727 32-33 23.76601665486419 24.58466340269444 27.385454541999376 24.263865400441993 34-35 23.938750908261422 24.541607094354806 27.977417699264716 23.542224298119056 36-37 24.187946758404294 24.3753679366513 27.42082804122166 24.01585726372274 38-39 24.172194284940186 24.21022147529753 27.55403519188087 24.06354904788141 40-41 23.848009602696965 24.137886334332233 27.88783017245463 24.12627389051617 42-43 24.42428136890284 24.19114340424729 27.30521941003356 24.07935581681631 44-45 24.11163383658546 24.68380319692436 26.87506714865972 24.329495817830463 46-47 24.08455460814484 24.68921611864212 26.896286284814764 24.32994298839828 48-49 24.917121357299898 24.832101436970355 26.170505152843788 24.08027205288596 50-51 24.03148757237756 25.245615742537392 25.871330321835046 24.85156636325 52-53 23.949174981866967 25.188641552104663 25.737339234992753 25.12484423103562 54-55 24.09176233189274 25.158783710870104 25.66968373024001 25.079770226997145 56-57 24.375787080728394 24.82021387895441 25.625241150845646 25.178757889471555 58-59 23.798974005636143 24.87436367404963 26.431008034779506 24.895654285534725 60-61 24.007224283866492 25.01432382388884 26.012138838405413 24.966313053839254 62-63 23.73439633006054 24.977477560021267 25.867699312225696 25.420426797692496 64-65 23.610704460657978 24.749137957597135 26.081752418897157 25.558405162847723 66-67 24.238740171332076 24.83386603977116 25.862357995286338 25.06503579361043 68-69 23.942492208801806 24.333874442494448 26.116256527279134 25.60737682142461 70-71 23.42325099805581 24.57904923705792 26.04745466754571 25.950245097340556 72-73 24.135164773858687 24.617037077155356 25.983280795678404 25.264517353307554 74-75 24.306935282649324 24.532682276343042 25.85612759001273 25.304254850994905 76-77 23.71719823968654 24.80749880176683 25.95725969130039 25.51804326724624 78-79 23.860984584949733 24.908472566406637 25.815748453571025 25.414794395072605 80-81 23.425383399414972 24.924849228457056 26.015975108536033 25.633792263591936 82-83 23.599506019806714 24.756494993889724 25.950934268952885 25.693064717350673 84-85 23.722064471256452 24.955548976754343 25.445273762514596 25.877112789474605 86-87 23.353615406448426 24.677196458795102 25.875483925350785 26.093704209405686 88-89 23.42907253698438 24.272790218453753 25.971179692625196 26.32695755193667 90-91 23.60318875559084 24.482893447052827 25.821613450495175 26.092304346861162 92-93 23.339354523443106 24.526785699136187 25.717271019728077 26.416588757692626 94-95 22.63612403783681 24.58201601086784 26.36353437399744 26.418325577297914 96-97 23.311335469593853 24.37076485809369 25.96370963308524 26.354190039227216 98-99 23.003396045959487 24.540236133717254 25.781668138752618 26.674699681570637 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 192.0 1 171.5 2 411.5 3 688.0 4 535.0 5 478.5 6 919.5 7 2400.0 8 4415.0 9 4545.0 10 2898.0 11 1828.0 12 1683.5 13 1821.5 14 2120.0 15 2638.5 16 3229.5 17 3868.0 18 4759.0 19 5965.0 20 7554.5 21 9494.5 22 11898.0 23 14046.0 24 16596.5 25 20402.0 26 24834.0 27 28844.0 28 33287.0 29 40898.0 30 49018.5 31 59014.0 32 69921.0 33 79305.0 34 86853.0 35 95960.5 36 104290.0 37 116688.5 38 135545.0 39 139232.0 40 141924.5 41 152277.0 42 165955.0 43 188043.0 44 212132.5 45 244603.0 46 282312.0 47 314538.5 48 334097.5 49 348456.5 50 372720.0 51 368790.5 52 349694.0 53 346705.5 54 327209.5 55 307958.5 56 278150.0 57 246176.5 58 235353.5 59 207763.5 60 170894.0 61 133765.5 62 105626.0 63 83291.5 64 59521.5 65 43183.5 66 30119.0 67 22419.0 68 17717.5 69 15395.0 70 13797.0 71 12090.0 72 12255.0 73 10365.5 74 6760.5 75 5282.0 76 4012.0 77 2538.5 78 1356.5 79 881.5 80 599.0 81 428.5 82 292.0 83 188.0 84 121.0 85 93.0 86 48.0 87 16.5 88 10.5 89 8.0 90 5.0 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05506918125280554 2 0.009053769754898027 3 0.0 4 0.0 5 0.0020903756255686605 6 0.0 7 0.0 8 0.0 9 0.004452228605107276 10-11 0.0 12-13 0.0 14-15 0.002694412738151812 16-17 0.004472589406655023 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.447587156786452E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.2396881702521358E-4 54-55 0.0 56-57 0.0010791224820305746 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0017646028008047132 70-71 3.25772824763947E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.904632448846541E-4 80-81 9.976792758395877E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.0541202321620036E-4 92-93 8.076451280606187E-4 94-95 5.225939063921651E-4 96-97 2.4432961857296027E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7367097.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.816944194700064 #Duplication Level Percentage of deduplicated Percentage of total 1 70.32847972402058 20.969803552292166 2 14.780980189158695 8.814473228862239 3 5.48858324632908 4.909583410912796 4 2.632890432831929 3.140189884260373 5 1.4911372594409122 2.223057822569383 6 0.9343248008234791 1.6715226267526755 7 0.6632504196360691 1.384327052458005 8 0.46381830556983683 1.1063715626924933 9 0.3679451948898096 0.9873901208463742 >10 2.295296719129943 13.000032910619455 >50 0.25250289264167386 5.286149209949997 >100 0.23863798496878033 15.21425181732196 >500 0.039191349731808385 8.132750786756956 >1k 0.022362084741707113 11.454239619278974 >5k 4.149665209762168E-4 0.782003219062794 >10k+ 1.8442956487831858E-4 0.9238531753633795 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19551 0.2653826873733304 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 18624 0.25279971201682294 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 16626 0.22567912435522433 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12399 0.16830238559367414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9815 0.13322751146075584 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 8023 0.1089031405450478 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.3573867698497794E-5 0.0 5 0.0 0.0 0.0 6.786933849248897E-5 0.0 6 2.7147735396995587E-5 0.0 0.0 6.786933849248897E-5 0.0 7 2.7147735396995587E-5 0.0 0.0 6.786933849248897E-5 0.0 8 2.7147735396995587E-5 0.0 0.0 8.144320619098676E-5 0.0 9 2.7147735396995587E-5 1.3573867698497794E-5 0.0 8.144320619098676E-5 0.0 10-11 2.7147735396995587E-5 1.3573867698497794E-5 0.0 9.501707388948455E-5 0.0 12-13 2.7147735396995587E-5 1.3573867698497794E-5 0.0 1.2895174313572904E-4 0.0 14-15 2.7147735396995587E-5 1.3573867698497794E-5 0.0 1.4931254468347573E-4 0.0 16-17 4.072160309549338E-5 1.3573867698497794E-5 0.0 1.4931254468347573E-4 0.0 18-19 4.7508536944742276E-5 1.3573867698497794E-5 0.0 1.628864123819735E-4 1.3573867698497794E-5 20-21 5.4295470793991175E-5 1.3573867698497794E-5 0.0 2.714773539699559E-4 1.3573867698497794E-5 22-23 5.4295470793991175E-5 2.7147735396995587E-5 0.0 0.0010248270112365834 3.3934669246244486E-5 24-25 6.786933849248897E-5 2.7147735396995587E-5 0.0 0.0028437252828352874 4.072160309549338E-5 26-27 8.823014004023566E-5 2.7147735396995587E-5 0.0 0.004608328083640001 4.072160309549338E-5 28-29 9.501707388948455E-5 2.7147735396995587E-5 0.0 0.012759435636587924 4.072160309549338E-5 30-31 9.501707388948455E-5 2.7147735396995587E-5 0.0 0.03951352887032708 4.072160309549338E-5 32-33 1.0180400773873344E-4 2.7147735396995587E-5 0.0 0.09127068640469917 4.072160309549338E-5 34-35 1.2216480928648013E-4 2.7147735396995587E-5 0.0 0.1623706054094306 4.072160309549338E-5 36-37 1.2216480928648013E-4 2.7147735396995587E-5 0.0 0.2748504600930326 4.072160309549338E-5 38-39 1.2216480928648013E-4 2.7147735396995587E-5 0.0 0.44385868680702856 4.072160309549338E-5 40-41 1.2216480928648013E-4 5.4295470793991175E-5 0.0 0.6422543370882723 4.072160309549338E-5 42-43 1.3573867698497794E-4 5.4295470793991175E-5 0.0 0.8265195910953799 4.072160309549338E-5 44-45 1.4252561083422685E-4 5.4295470793991175E-5 0.0 1.0479772425963714 4.072160309549338E-5 46-47 1.560994785327246E-4 5.4295470793991175E-5 0.0 1.291309181893492 4.072160309549338E-5 48-49 1.628864123819735E-4 5.4295470793991175E-5 0.0 1.5509433362965086 4.072160309549338E-5 50-51 1.6967334623122242E-4 6.108240464324007E-5 0.0 1.8325603694372423 4.072160309549338E-5 52-53 1.7646028008047132E-4 6.786933849248897E-5 0.0 2.1073768948610283 4.7508536944742276E-5 54-55 1.7646028008047132E-4 6.786933849248897E-5 0.0 2.384840324486022 6.108240464324007E-5 56-57 1.7646028008047132E-4 6.786933849248897E-5 0.0 2.700514191682287 6.786933849248897E-5 58-59 1.7646028008047132E-4 6.786933849248897E-5 0.0 3.0483038298531975 6.786933849248897E-5 60-61 2.171818831759647E-4 6.786933849248897E-5 0.0 3.395007558608228 8.144320619098676E-5 62-63 2.239688170252136E-4 6.786933849248897E-5 0.0 3.748267465461633 8.144320619098676E-5 64-65 2.3075575087446248E-4 6.786933849248897E-5 0.0 4.121195906610161 8.144320619098676E-5 66-67 2.3075575087446248E-4 6.786933849248897E-5 0.0 4.528561250109778 8.144320619098676E-5 68-69 2.3075575087446248E-4 6.786933849248897E-5 0.0 4.967567279214594 8.144320619098676E-5 70-71 2.3075575087446248E-4 6.786933849248897E-5 0.0 5.447539241033477 8.144320619098676E-5 72-73 2.3075575087446248E-4 6.786933849248897E-5 0.0 5.9190818310115905 8.144320619098676E-5 74-75 2.3075575087446248E-4 6.786933849248897E-5 0.0 6.391214884234591 8.144320619098676E-5 76-77 2.3075575087446248E-4 6.786933849248897E-5 0.0 6.904347804840903 8.144320619098676E-5 78-79 2.3075575087446248E-4 6.786933849248897E-5 0.0 7.427172466983942 8.144320619098676E-5 80-81 2.5111655242220917E-4 6.786933849248897E-5 0.0 7.994702662391984 8.823014004023566E-5 82-83 2.579034862714581E-4 6.786933849248897E-5 0.0 8.589421314800116 9.501707388948455E-5 84-85 2.714773539699559E-4 6.786933849248897E-5 0.0 9.180916173629857 1.0180400773873344E-4 86-87 2.9183815551770255E-4 6.786933849248897E-5 0.0 9.788645378227 1.0859094158798235E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 45375 0.0 27.780024 1 TATCACG 495 0.0 23.48699 2 CAACGCA 54640 0.0 22.782932 5 ATCAACG 54980 0.0 22.726622 3 AACGCAG 58035 0.0 21.92201 6 TATCAAC 57985 0.0 21.782448 2 AGTACTT 28740 0.0 21.739637 12-13 TAGGCAT 4855 0.0 21.455109 5 TCAACGC 58720 0.0 21.34247 4 CGCAGAG 61185 0.0 20.071947 8 TAGTACT 4265 0.0 19.952806 4 CTAGGCA 5370 0.0 19.917072 4 ACGCAGA 61715 0.0 19.906565 7 GAGTACT 26965 0.0 19.41956 12-13 TACTTTT 32875 0.0 19.188877 14-15 GCAGAGT 66095 0.0 18.539024 9 GGTATCA 31960 0.0 18.51913 1 CTAGTAC 5015 0.0 17.988821 3 CGTTAAC 1355 0.0 17.856829 1 CTGTGCG 5065 0.0 17.62774 9 >>END_MODULE