FastQCFastQC Report
Fri 27 May 2016
ERR765401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13043990
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA159710.12243952962245448No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT150840.11563946307839856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142660.10936837578072355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA430750.024.2978631
GTACATG698300.020.6164261
CAACGCA469900.020.2741765
TACATGG688950.020.098042
ATCAACG476950.019.9548073
AACGCAG492950.019.7357446
CATGGGG312900.019.6126674
TATCAAC548600.019.0517982
GTACTAG48050.018.8602391
ACATGGG691800.018.5220723
GTATAAG54850.018.4911
TCAACGC522150.018.3173374
ACGCAGA515750.018.2351077
CGCAGAG524550.017.8933878
GTACATA30650.017.6177581
GCAGAGT531050.016.9318719
TATATAC54200.016.8061833
GTATTAG48350.016.6066841
GTACAAG56050.016.5034161
TAGTACT48700.016.3902874
Produced by FastQC (version 0.11.5)