##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765401_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13043990
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.041125606505375	33.0	33.0	33.0	27.0	33.0
2	32.02369175382686	33.0	33.0	33.0	27.0	33.0
3	32.0039866635899	33.0	33.0	33.0	27.0	33.0
4	35.793343754480034	37.0	37.0	37.0	33.0	37.0
5	35.81007199484207	37.0	37.0	37.0	33.0	37.0
6	35.81463785237492	37.0	37.0	37.0	33.0	37.0
7	35.75837324315643	37.0	37.0	37.0	33.0	37.0
8	35.72613893448247	37.0	37.0	37.0	33.0	37.0
9	35.74285299206761	37.0	37.0	37.0	33.0	37.0
10-11	35.70872934585199	37.0	37.0	37.0	33.0	37.0
12-13	35.687021034208094	37.0	37.0	37.0	33.0	37.0
14-15	37.442281771145176	40.0	37.0	40.0	33.0	40.0
16-17	37.48200128948274	40.0	37.0	40.0	33.0	40.0
18-19	37.523474987331326	40.0	37.0	40.0	33.0	40.0
20-21	37.53056361588747	40.0	37.0	40.0	33.0	40.0
22-23	37.50914306128723	40.0	37.0	40.0	33.0	40.0
24-25	37.50606927021563	40.0	37.0	40.0	33.0	40.0
26-27	37.48813829204101	40.0	37.0	40.0	33.0	40.0
28-29	37.44187740867633	40.0	37.0	40.0	33.0	40.0
30-31	37.38141968063453	40.0	37.0	40.0	33.0	40.0
32-33	37.31160722294329	40.0	37.0	40.0	33.0	40.0
34-35	37.21079113829434	40.0	37.0	40.0	33.0	40.0
36-37	37.119734452418314	40.0	37.0	40.0	33.0	40.0
38-39	37.007206997245476	40.0	37.0	40.0	33.0	40.0
40-41	36.908155671692484	40.0	37.0	40.0	33.0	40.0
42-43	36.76195684755968	38.5	37.0	40.0	33.0	40.0
44-45	36.607993566385744	37.0	37.0	40.0	33.0	40.0
46-47	36.42490050973667	37.0	37.0	40.0	33.0	40.0
48-49	36.239030120384946	37.0	37.0	40.0	33.0	40.0
50-51	36.016406214662844	37.0	37.0	40.0	27.0	40.0
52-53	35.86866894255515	37.0	37.0	40.0	27.0	40.0
54-55	35.69525804604266	37.0	37.0	40.0	27.0	40.0
56-57	35.5172206127113	37.0	35.0	40.0	27.0	40.0
58-59	35.302995095825736	37.0	33.0	40.0	27.0	40.0
60-61	35.14409433003245	37.0	33.0	40.0	27.0	40.0
62-63	34.92559128763515	37.0	33.0	37.0	27.0	40.0
64-65	34.7183060934576	37.0	33.0	37.0	27.0	40.0
66-67	34.529431676963874	37.0	33.0	37.0	27.0	40.0
68-69	34.295234816954014	37.0	33.0	37.0	27.0	40.0
70-71	34.101329731163545	37.0	33.0	37.0	27.0	40.0
72-73	33.89488354406896	37.0	33.0	37.0	27.0	37.0
74-75	33.68673243386418	37.0	33.0	37.0	27.0	37.0
76-77	33.503955576476216	37.0	33.0	37.0	27.0	37.0
78-79	33.344233627900664	37.0	33.0	37.0	27.0	37.0
80-81	33.18076708123818	37.0	33.0	37.0	27.0	37.0
82-83	33.03567152382055	37.0	33.0	37.0	27.0	37.0
84-85	32.89446580379163	37.0	33.0	37.0	27.0	37.0
86-87	32.79024201950477	37.0	33.0	37.0	27.0	37.0
88-89	32.70977936965606	37.0	33.0	37.0	27.0	37.0
90-91	32.58876785400786	33.0	33.0	37.0	22.0	37.0
92-93	32.50087415737056	33.0	33.0	37.0	22.0	37.0
94-95	32.395845749651755	33.0	33.0	37.0	22.0	37.0
96-97	32.311816821386714	33.0	33.0	37.0	22.0	37.0
98-99	32.21579049048643	33.0	33.0	37.0	22.0	37.0
100	32.13899704001613	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	38.0
5	96.0
6	450.0
7	976.0
8	1686.0
9	2767.0
10	3480.0
11	5075.0
12	8615.0
13	15851.0
14	25120.0
15	29947.0
16	34057.0
17	40155.0
18	47277.0
19	55107.0
20	64195.0
21	76114.0
22	91689.0
23	92745.0
24	87324.0
25	89898.0
26	100574.0
27	117421.0
28	143269.0
29	179897.0
30	232334.0
31	303829.0
32	407287.0
33	555525.0
34	768742.0
35	1143780.0
36	2113899.0
37	4144409.0
38	2058421.0
39	1935.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.875350184305915	19.32377044780341	12.861621947265533	24.939257420625147
2	16.639812176756184	21.041099137648498	36.8820998067044	25.436988878890915
3	18.221660428905583	25.687800077016586	29.496909025131433	26.59363046894639
4	13.403237812969804	17.22656947759083	36.53779250060756	32.83240020883181
5	14.502819559319994	37.02763900287533	33.38585930914568	15.083682128658996
6	31.716092833101094	36.78514084057857	17.393380603202953	14.105385723117383
7	27.592592450622856	31.63256028255158	22.221107191894504	18.55374007493106
8	26.972966093963578	32.29482696628869	20.32608120674732	20.406125733000408
9	27.04456228500635	14.426620995569609	19.872707660769443	38.6561090586546
10-11	25.483402701167357	25.060491460051715	27.348928510371444	22.107177328409485
12-13	26.39638638177429	23.24694361157897	26.73594505975549	23.62072494689125
14-15	24.222185917634363	24.124941668399906	24.43727782339702	27.21559459056871
16-17	23.851812649579255	26.50419522455753	25.26032679285326	24.38366533300995
18-19	24.21430120411771	25.857489525990275	26.86601750953961	23.062191760352405
20-21	25.536641070065475	25.155218612661407	25.768670572931146	23.539469744341982
22-23	25.022715332882285	24.776594538151983	25.73649707750727	24.464193051458462
24-25	24.41819196057112	25.403085726433154	25.91081660721015	24.267905705785573
26-27	24.551772239798677	25.43194690431757	25.75367594194874	24.26260491393501
28-29	24.22340239738281	25.21446292975326	26.261840474478788	24.300294198385142
30-31	24.626606981410838	25.10049308407165	26.04064132567997	24.232258608837547
32-33	24.3329927618557	25.30129913335496	25.62289812430505	24.742809980484292
34-35	24.75887175633513	25.227653342628788	25.9911722619306	24.022302639105487
36-37	24.7702299562492	25.11970555683464	25.58732477463292	24.522739712283244
38-39	24.675375184314728	24.837689509905015	25.8698097885829	24.617125517197362
40-41	24.518326319353452	25.029924187966657	26.158992091455026	24.292757401224872
42-43	24.881681304946284	25.050834843488968	25.766371147492396	24.301112704072352
44-45	24.429469830188367	25.25612795356804	25.542864707606284	24.771537508637305
46-47	24.30905530472067	25.123943146910367	25.753844308386505	24.813157239982463
48-49	24.86054436455051	25.258988489460116	25.366687698293127	24.513779447696248
50-51	24.27435916363173	25.464524521895182	25.187565488065232	25.073550826407853
52-53	24.301730787408474	25.34365058076698	25.170995568489396	25.183623063335148
54-55	24.44652468111703	25.25440986821253	25.29041278434518	25.008652666325265
56-57	24.445414499510132	24.9706516371961	25.012520894362307	25.57141296893146
58-59	24.11108792286182	24.937654416291558	25.516552766855394	25.434704893991224
60-61	24.325500091386246	25.018670328968245	25.29742330669109	25.35840627295442
62-63	24.015095858895705	24.913217024539875	25.243554447852762	25.828132668711657
64-65	23.896029219072645	24.839609064685682	25.392355592175342	25.87200612406633
66-67	24.371333405293605	24.928190870849015	25.144428290663	25.55604743319438
68-69	24.261695109286563	24.51745552777935	25.33007825650705	25.890771106427042
70-71	23.75135548098277	24.55261218159451	25.465808592605683	26.23022374481704
72-73	24.238713201887972	24.62028597328569	25.437661997891137	25.70333882693521
74-75	24.203013983139243	24.685356358370864	25.452967965787632	25.65866169270226
76-77	23.922619855698397	24.80865886330464	25.52016916743941	25.748552113557555
78-79	24.031264055535686	24.80236591443175	25.46191123111815	25.704458798914416
80-81	23.672726512799834	24.660067115187694	25.831241136322287	25.83596523569019
82-83	23.928397351802833	24.576011677408673	25.657192587259374	25.838398383529125
84-85	23.814702896656275	24.63560098870972	25.35258501761669	26.197111097017313
86-87	23.554132077417037	24.490160719878926	25.659545658998127	26.29616154370591
88-89	23.756959459604538	24.167659980534527	25.67827795876914	26.3971026010918
90-91	23.856943569271788	24.326045911467055	25.517642234700315	26.299368284560842
92-93	23.788604893725005	24.319529408409025	25.555002554445966	26.336863143420008
94-95	23.225827648701465	24.29738042088224	25.945936123182083	26.53085580723421
96-97	23.64459195651558	24.281824628425348	25.564068629610276	26.509514785448797
98-99	23.382575775291674	24.35463697531992	25.578711912239815	26.684075337148595
100	23.37424556508576	24.366976953365608	25.52834786171437	26.73042961983427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	275.0
2	394.5
3	513.5
4	453.5
5	487.0
6	655.5
7	1190.0
8	1891.0
9	1934.0
10	1514.5
11	1320.0
12	1435.0
13	1616.5
14	1831.0
15	2206.5
16	2639.0
17	3094.0
18	3768.5
19	4548.0
20	5543.0
21	7040.5
22	8759.0
23	11019.0
24	14283.5
25	19354.5
26	27118.0
27	36108.5
28	44606.0
29	58158.5
30	73391.5
31	87405.5
32	106017.5
33	126251.5
34	150248.0
35	168592.0
36	184946.5
37	210005.0
38	226236.0
39	240977.5
40	256400.5
41	270659.0
42	285531.0
43	316212.0
44	360798.0
45	405432.0
46	462383.0
47	514643.0
48	555615.5
49	586051.5
50	616610.5
51	625553.5
52	612842.5
53	612450.5
54	596496.0
55	560516.0
56	527879.0
57	485362.0
58	450999.0
59	410620.0
60	343190.0
61	276673.5
62	223470.0
63	174790.0
64	130466.0
65	103118.0
66	82026.5
67	62450.5
68	49378.5
69	41030.0
70	35864.0
71	32039.5
72	27984.0
73	27976.5
74	22691.0
75	16386.0
76	12474.5
77	8984.0
78	6636.0
79	4281.0
80	3059.0
81	2337.0
82	1710.5
83	1291.0
84	959.5
85	621.0
86	402.5
87	251.5
88	139.0
89	83.0
90	60.0
91	40.5
92	24.0
93	15.0
94	13.5
95	12.0
96	7.0
97	5.5
98	9.5
99	8.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02847288291389368
2	0.001816928715830049
3	3.6031919681017846E-4
4	0.0
5	0.0021849142785298056
6	1.6866004957072185E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.3765734257692625E-4
16-17	0.004051674372642113
18-19	0.006681237872767459
20-21	0.019510901188976686
22-23	0.024831359116344005
24-25	0.03226006766334534
26-27	0.031700422953406124
28-29	0.04744713849059989
30-31	0.04716731613563028
32-33	0.05802289023527311
34-35	0.05204312484140206
36-37	0.05607563329932023
38-39	0.060288301355643475
40-41	0.043119474945932955
42-43	0.049267900389374716
44-45	0.0465578400474088
46-47	0.04411226932863334
48-49	0.03754219376126477
50-51	0.03739269962641799
52-53	0.03819766804482371
54-55	0.05785039700275759
56-57	0.04434226030532069
58-59	0.04428859574409364
60-61	0.04540788516397207
62-63	0.030588799899417277
64-65	0.0328733769345116
66-67	0.03363618034052464
68-69	0.03106411458457113
70-71	0.043863112437222046
72-73	0.03277754736089187
74-75	0.04162836678040998
76-77	0.04615152265526116
78-79	0.04071223605660538
80-81	0.0342494896116909
82-83	0.021009675720389236
84-85	0.033494352571567444
86-87	0.027832741362113896
88-89	0.02657162417327827
90-91	0.0223282906533967
92-93	0.015509058194616832
94-95	0.015551223207009511
96-97	0.014516263811916445
98-99	0.01109323144221975
100	0.006010430857429361
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.304399E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.300358356025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.82179336286443	25.706354018375173
2	14.12312258299403	9.971025765715174
3	5.087087455289297	5.387280304804545
4	2.313544471824737	3.2667579571205514
5	1.299381569525274	2.293431752272819
6	0.8151112176972538	1.7264230850837379
7	0.5581537610194914	1.379211944722583
8	0.40312417202405465	1.1384342187539997
9	0.30022648318291556	0.9538292199893429
>10	1.8659662438758204	12.680768591979245
>50	0.20666420502715438	5.113345099230999
>100	0.16447653285861874	11.868825814301196
>500	0.023462633533023382	5.85839077819408
>1k	0.017189044941040583	10.983772115520866
>5k	6.309886546990377E-4	1.3240508347449869
>10k+	6.527468841714184E-5	0.34809849919063934
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	15971	0.12243952962245448	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	15084	0.11563946307839856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14266	0.10936837578072355	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.666365889578266E-6
2	0.0	7.666365889578266E-6	0.0	0.0	7.666365889578266E-6
3	0.0	7.666365889578266E-6	0.0	7.666365889578266E-6	7.666365889578266E-6
4	0.0	7.666365889578266E-6	0.0	2.2999097668734795E-5	7.666365889578266E-6
5	0.0	7.666365889578266E-6	0.0	3.833182944789133E-5	7.666365889578266E-6
6	7.666365889578266E-6	7.666365889578266E-6	0.0	3.833182944789133E-5	7.666365889578266E-6
7	7.666365889578266E-6	7.666365889578266E-6	0.0	3.833182944789133E-5	7.666365889578266E-6
8	7.666365889578266E-6	7.666365889578266E-6	0.0	3.833182944789133E-5	7.666365889578266E-6
9	7.666365889578266E-6	7.666365889578266E-6	0.0	5.366456122704786E-5	7.666365889578266E-6
10-11	1.5332731779156532E-5	7.666365889578266E-6	0.0	6.516411006141526E-5	7.666365889578266E-6
12-13	3.8331829447891335E-5	7.666365889578266E-6	0.0	8.433002478536093E-5	1.5332731779156532E-5
14-15	6.516411006141526E-5	7.666365889578266E-6	0.0	1.1116230539888486E-4	1.9165914723945664E-5
16-17	6.899729300620439E-5	7.666365889578266E-6	0.0	1.1499548834367398E-4	2.2999097668734795E-5
18-19	7.283047595099352E-5	7.666365889578266E-6	0.0	1.2266185423325226E-4	2.683228061352393E-5
20-21	9.199639067493918E-5	7.666365889578266E-6	0.0	1.533273177915653E-4	3.0665463558313065E-5
22-23	9.966275656451745E-5	7.666365889578266E-6	0.0	5.979765393871047E-4	3.0665463558313065E-5
24-25	1.1116230539888486E-4	7.666365889578266E-6	0.0	0.0021504156320267037	3.0665463558313065E-5
26-27	1.9549233018424577E-4	7.666365889578266E-6	0.0	0.003814017030065187	3.0665463558313065E-5
28-29	1.993255131290349E-4	7.666365889578266E-6	0.0	0.01565088596357403	3.0665463558313065E-5
30-31	1.993255131290349E-4	7.666365889578266E-6	0.0	0.05732141775637669	3.0665463558313065E-5
32-33	1.993255131290349E-4	7.666365889578266E-6	0.0	0.1345332218132642	3.0665463558313065E-5
34-35	2.0315869607382404E-4	7.666365889578266E-6	0.0	0.23599757436183255	3.0665463558313065E-5
36-37	2.0699187901861317E-4	7.666365889578266E-6	0.0	0.39901901182076954	3.44986465031022E-5
38-39	2.0699187901861317E-4	7.666365889578266E-6	0.0	0.6446340421910781	3.833182944789133E-5
40-41	2.108250619634023E-4	1.5332731779156532E-5	0.0	0.9270936270266996	3.833182944789133E-5
42-43	2.1849142785298058E-4	1.5332731779156532E-5	0.0	1.189344671377393	3.833182944789133E-5
44-45	2.2999097668734798E-4	1.5332731779156532E-5	0.0	1.4931090870201524	3.833182944789133E-5
46-47	2.3765734257692622E-4	1.5332731779156532E-5	0.0	1.8359834682485956	3.833182944789133E-5
48-49	2.3765734257692622E-4	1.5332731779156532E-5	0.0	2.1884753054855146	4.599819533746959E-5
50-51	2.453237084665045E-4	1.5332731779156532E-5	0.0	2.5519875436887025	4.599819533746959E-5
52-53	2.453237084665045E-4	1.5332731779156532E-5	0.0	2.902018477475067	5.749774417183699E-5
54-55	2.453237084665045E-4	1.5332731779156532E-5	0.0	3.2598844372005806	7.283047595099352E-5
56-57	2.453237084665045E-4	1.5332731779156532E-5	0.0	3.6687968942018507	8.049684184057179E-5
58-59	2.453237084665045E-4	1.5332731779156532E-5	0.0	4.108114158321189	8.816320773015005E-5
60-61	2.453237084665045E-4	1.5332731779156532E-5	0.0	4.562334837729867	9.966275656451745E-5
62-63	2.453237084665045E-4	1.5332731779156532E-5	0.0	5.022876435814501	9.966275656451745E-5
64-65	2.453237084665045E-4	1.5332731779156532E-5	0.0	5.487703532431411	1.0349593950930659E-4
66-67	2.453237084665045E-4	1.5332731779156532E-5	0.0	5.992756050870938	1.0732912245409572E-4
68-69	2.529900743560828E-4	1.5332731779156532E-5	0.0	6.5555554703737124	1.0732912245409572E-4
70-71	2.529900743560828E-4	1.5332731779156532E-5	0.0	7.166771823652119	1.0732912245409572E-4
72-73	2.529900743560828E-4	1.5332731779156532E-5	0.0	7.765898317922661	1.0732912245409572E-4
74-75	2.529900743560828E-4	1.5332731779156532E-5	0.0	8.366726745420689	1.0732912245409572E-4
76-77	2.529900743560828E-4	1.5332731779156532E-5	0.0	9.00668047123618	1.1882867128846312E-4
78-79	2.568232573008719E-4	1.5332731779156532E-5	0.0	9.687204605339318	1.2266185423325226E-4
80-81	2.60656440245661E-4	1.5332731779156532E-5	0.0	10.42193377946472	1.3799458601240877E-4
82-83	2.644896231904501E-4	1.5332731779156532E-5	0.0	11.187734734540582	1.4566095190198704E-4
84-85	2.7598917202481754E-4	1.5332731779156532E-5	0.0	11.936746348318268	1.4566095190198704E-4
86-87	2.7598917202481754E-4	1.5332731779156532E-5	0.0	12.70765310307659	1.4566095190198704E-4
88	2.7598917202481754E-4	1.5332731779156532E-5	0.0	13.302961747134121	1.4566095190198704E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	43075	0.0	24.297863	1
GTACATG	69830	0.0	20.616426	1
CAACGCA	46990	0.0	20.274176	5
TACATGG	68895	0.0	20.09804	2
ATCAACG	47695	0.0	19.954807	3
AACGCAG	49295	0.0	19.735744	6
CATGGGG	31290	0.0	19.612667	4
TATCAAC	54860	0.0	19.051798	2
GTACTAG	4805	0.0	18.860239	1
ACATGGG	69180	0.0	18.522072	3
GTATAAG	5485	0.0	18.491	1
TCAACGC	52215	0.0	18.317337	4
ACGCAGA	51575	0.0	18.235107	7
CGCAGAG	52455	0.0	17.893387	8
GTACATA	3065	0.0	17.617758	1
GCAGAGT	53105	0.0	16.931871	9
TATATAC	5420	0.0	16.806183	3
GTATTAG	4835	0.0	16.606684	1
GTACAAG	5605	0.0	16.503416	1
TAGTACT	4870	0.0	16.390287	4
>>END_MODULE