FastQCFastQC Report
Fri 27 May 2016
ERR765400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11712924
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT603120.5149183927087719No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA583600.49825304082908756No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG232080.19814010574985375No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC173390.14803306159930688No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC164990.140861496241246No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT164070.1400760390829822No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG161660.1380184828314433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158970.13572187440130237No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG155770.13298984950299345No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA130030.11101412422722115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG52700.020.2462771
GTATAAG49900.020.0636061
GTACTAG49050.019.357191
TAGTACT55550.019.2886124
CATGGGG290750.019.0242234
ATGGGAG136850.018.3014815
GTACATA33550.017.932771
CTATTGA46150.017.5099359
TAGGCAT69000.017.16155
GGGCTAT51150.017.0864056
ACGTTAA12750.016.950738
CGTTAAC16150.016.8805051
CTAGTAC67500.016.8488083
TACATGA44250.016.781372
GTGATCG33900.016.7697458
TCTAACG24950.016.764982
GTACATG1463550.016.7613771
CTAACGC24200.016.7009323
CATGGGT78750.016.6496014
CTGTGCG63550.016.5599989
Produced by FastQC (version 0.11.5)