##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765400_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11712924
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44338587017213	33.0	33.0	33.0	27.0	33.0
2	31.30898484443338	33.0	33.0	33.0	27.0	33.0
3	31.300992903223822	33.0	33.0	33.0	27.0	33.0
4	34.848811876522035	37.0	37.0	37.0	33.0	37.0
5	35.08536800887635	37.0	37.0	37.0	33.0	37.0
6	35.09156722949795	37.0	37.0	37.0	33.0	37.0
7	34.959998801324076	37.0	37.0	37.0	33.0	37.0
8	34.93276119609416	37.0	37.0	37.0	33.0	37.0
9	34.62800808747671	37.0	37.0	37.0	27.0	37.0
10-11	34.637265255029405	37.0	37.0	37.0	27.0	37.0
12-13	34.62112282979041	37.0	37.0	37.0	27.0	37.0
14-15	35.818330247852714	37.0	37.0	40.0	27.0	40.0
16-17	36.05249999914624	37.0	37.0	40.0	27.0	40.0
18-19	35.865777623076866	37.0	37.0	40.0	27.0	40.0
20-21	35.846390107201245	37.0	37.0	40.0	27.0	40.0
22-23	36.015499588318	37.0	37.0	40.0	27.0	40.0
24-25	36.03998224525319	37.0	37.0	40.0	27.0	40.0
26-27	35.71952754922682	37.0	37.0	40.0	27.0	40.0
28-29	35.584619391366324	37.0	35.0	40.0	27.0	40.0
30-31	35.37218588629108	37.0	33.0	40.0	27.0	40.0
32-33	35.30685629822237	37.0	33.0	40.0	27.0	40.0
34-35	35.250979174798715	37.0	33.0	40.0	27.0	40.0
36-37	35.01983164067316	37.0	33.0	40.0	27.0	40.0
38-39	34.895562329269794	37.0	33.0	40.0	27.0	40.0
40-41	35.014377110275795	37.0	33.0	40.0	27.0	40.0
42-43	34.91279235654564	37.0	33.0	40.0	27.0	40.0
44-45	34.841566034237054	37.0	33.0	40.0	27.0	40.0
46-47	34.76698862726335	37.0	33.0	40.0	27.0	40.0
48-49	34.58238216178983	37.0	33.0	40.0	24.5	40.0
50-51	34.425658315549555	37.0	33.0	40.0	22.0	40.0
52-53	34.31680411313178	37.0	33.0	40.0	22.0	40.0
54-55	34.17502128418147	37.0	33.0	40.0	24.5	40.0
56-57	33.965972800643115	37.0	33.0	38.5	22.0	40.0
58-59	33.77336653938846	37.0	33.0	37.0	22.0	40.0
60-61	33.49497132398366	37.0	33.0	37.0	22.0	40.0
62-63	33.258924287393995	37.0	33.0	37.0	22.0	40.0
64-65	33.07388095406408	37.0	33.0	37.0	22.0	40.0
66-67	32.85251735604193	37.0	33.0	37.0	22.0	40.0
68-69	32.655375549265074	37.0	33.0	37.0	22.0	40.0
70-71	32.41050919480055	35.0	33.0	37.0	22.0	37.0
72-73	32.16603181238092	33.0	33.0	37.0	22.0	37.0
74-75	31.939094243247887	33.0	33.0	37.0	22.0	37.0
76-77	31.725924927029325	33.0	33.0	37.0	22.0	37.0
78-79	31.53174655619724	33.0	30.0	37.0	22.0	37.0
80-81	31.38497756836807	33.0	27.0	37.0	22.0	37.0
82-83	31.241766658777944	33.0	27.0	37.0	22.0	37.0
84-85	31.059890596062946	33.0	27.0	37.0	22.0	37.0
86-87	30.842720015941367	33.0	27.0	37.0	15.0	37.0
88-89	30.679739918059745	33.0	27.0	37.0	15.0	37.0
90-91	30.572578077002802	33.0	27.0	37.0	15.0	37.0
92-93	30.450639140149804	33.0	27.0	37.0	15.0	37.0
94-95	30.301003532508194	33.0	27.0	37.0	15.0	37.0
96-97	30.196218894615896	33.0	27.0	37.0	15.0	37.0
98-99	30.045902671271495	33.0	27.0	37.0	15.0	37.0
100	29.935175452346485	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	167.0
8	1035.0
9	2611.0
10	4516.0
11	9148.0
12	17928.0
13	30224.0
14	40845.0
15	46408.0
16	53544.0
17	62437.0
18	72542.0
19	84979.0
20	98234.0
21	113903.0
22	129367.0
23	135778.0
24	139614.0
25	151883.0
26	173008.0
27	205706.0
28	249236.0
29	306422.0
30	378625.0
31	468223.0
32	596452.0
33	773278.0
34	997959.0
35	1318002.0
36	1858557.0
37	2335116.0
38	856181.0
39	990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.024026762535456	19.519399831487046	12.945848643115399	24.510724762862097
2	15.577794308773477	21.624503660934266	37.59886537895125	25.198836651341004
3	17.75505556167077	27.548877609759803	29.48320545611004	25.21286137245939
4	12.345185828243277	17.989967591637036	37.23316216418626	32.431684415933425
5	13.534172995900567	38.208386840111885	33.33892630200682	14.918513861980731
6	30.459508131371287	37.262505512403294	17.97627591427329	14.301710441952132
7	26.51526424072231	32.522909472710985	22.768627351828783	18.19319893473792
8	27.142536964581883	32.51676773627556	20.497300897139528	19.843394402003035
9	27.36710136257235	14.396345982776149	20.173346735562983	38.063205919088524
10-11	25.18304271866195	25.724642723374785	26.68590247699442	22.406412080968842
12-13	26.175105225769624	23.735691017807326	26.57872378245613	23.510479973966923
14-15	24.410683434624758	24.38390471025937	24.566045286139826	26.639366568976047
16-17	23.40699080736623	26.683014439346675	25.890397917708146	24.019596835578945
18-19	23.943989483534853	25.804281575596526	27.88200319826152	22.3697257426071
20-21	25.304622563105944	25.055414737380826	26.689913085494197	22.950049614019026
22-23	24.34612735011151	25.223552125942426	26.172133760035386	24.258186763910672
24-25	23.642999256529194	25.97947538937426	26.322824656176774	24.054700697919767
26-27	24.201053391548786	25.297751265072456	26.50277046042369	23.99842488295507
28-29	23.433451434110662	25.29785956736944	27.46691449309262	23.801774505427282
30-31	24.225427443961173	25.361219497008392	26.913016662497117	23.500336396533317
32-33	23.408702195342666	25.625465752833804	26.360224611906446	24.605607439917083
34-35	23.81785522981938	25.666217002630592	27.20058555123791	23.315342216312118
36-37	24.240337278435533	25.40503828411586	26.55106765290947	23.803556784539133
38-39	24.367596171817844	24.868094017278004	26.53822971348088	24.226080097423267
40-41	23.759751110962288	25.627772814351523	26.89291974719185	23.719556327494335
42-43	24.807020703085158	25.1143755032599	26.356749401897016	23.721854391757923
44-45	24.202728374753168	25.532638828052985	26.039343586454493	24.22528921073935
46-47	23.644229370732127	25.352453612272303	26.88730279382489	24.11601422317068
48-49	24.451891742270433	25.782948027866208	26.111724560377574	23.65343566948578
50-51	23.329382363844108	26.213498992500643	25.810302819456638	24.64681582419861
52-53	23.295253378348093	25.89962143445444	25.351563263714645	25.45356192348282
54-55	23.826906927766288	25.816538210270977	25.49540148984148	24.861153372121255
56-57	24.279509540060193	24.999039522496687	25.228892461011444	25.492558476431675
58-59	23.66097483429415	25.081136870690873	26.141427196146754	25.116461098868225
60-61	23.899096416915196	25.30217902890858	25.856357473163833	24.942367081012392
62-63	23.06901953391734	25.36895350391851	25.61352660469375	25.948500357470394
64-65	23.046631712070845	25.243980807842014	25.913737569377517	25.795649910709628
66-67	24.1222897062732	25.047116890270726	25.61207988702362	25.218513516432452
68-69	24.186098174417115	24.499033408473306	25.611725694788618	25.70314272232096
70-71	23.047909732101218	24.820820611440578	26.030588036920793	26.100681619537415
72-73	23.891252831761854	24.729854326772458	25.865743592733935	25.513149248731747
74-75	24.204376381327318	24.51611763045574	25.89888566633134	25.380620321885605
76-77	23.460638255320653	24.838245852473353	26.357995697825675	25.343120194380315
78-79	23.59278193868508	25.005857845823037	26.115405773801903	25.285954441689984
80-81	22.948355670771136	24.797279449141726	26.730550241861085	25.523814638226057
82-83	23.221673401904166	24.971701074383752	25.82339020125715	25.983235322454927
84-85	23.314659586429006	25.197878780285432	25.389686205835083	26.097775427450483
86-87	23.2837650933214	24.441472028211894	25.905257768693662	26.369505109773044
88-89	23.275997164603478	24.078842977217576	26.201407495615314	26.443752362563632
90-91	23.68437780135845	24.299907718324583	25.957665178414587	26.05804930190238
92-93	23.25472695880396	24.542554955838742	25.884581585997317	26.31813649935998
94-95	22.45229191220864	24.768291406941263	26.468868746211616	26.310547934638485
96-97	23.337421216678052	24.394503623983717	25.905795291813654	26.362279867524574
98-99	23.093600221234045	24.383271249998824	25.957954432036317	26.565174096730814
100	22.86576653639956	24.689008017945802	25.78936992731162	26.655855518343024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1477.0
1	1272.0
2	1316.5
3	1532.0
4	1259.5
5	1107.5
6	1443.5
7	2596.5
8	4156.0
9	4238.5
10	2932.0
11	2050.5
12	1928.5
13	2058.0
14	2347.5
15	2814.0
16	3406.5
17	4042.5
18	4896.5
19	6079.5
20	7492.5
21	9371.0
22	11809.5
23	15155.5
24	19611.5
25	25207.5
26	33273.0
27	42633.0
28	52413.0
29	66511.0
30	81522.5
31	95397.0
32	113733.0
33	132356.0
34	151152.0
35	167637.5
36	183734.5
37	205417.0
38	217162.0
39	224128.5
40	231819.5
41	237118.5
42	247862.0
43	274306.5
44	313987.5
45	356835.5
46	409418.0
47	461740.0
48	517000.5
49	570585.5
50	652853.0
51	655097.5
52	556446.0
53	505489.5
54	477280.5
55	448725.0
56	429365.0
57	401209.0
58	372623.0
59	344234.0
60	296886.5
61	229774.0
62	171500.5
63	130651.5
64	96369.5
65	78038.0
66	64944.0
67	47859.5
68	35654.5
69	28917.0
70	24561.5
71	22300.0
72	20347.5
73	21467.5
74	17733.5
75	12813.5
76	9573.5
77	7504.0
78	5995.0
79	3844.5
80	2715.5
81	2231.0
82	2178.0
83	2045.5
84	1630.5
85	1217.5
86	905.0
87	525.0
88	232.0
89	158.5
90	136.5
91	96.5
92	65.0
93	42.0
94	30.5
95	17.0
96	11.5
97	10.5
98	7.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04962040221553559
2	0.025057790864177042
3	0.022223315032181546
4	0.02283802063430105
5	0.015290801852722684
6	0.025339530931815145
7	0.04915937301394597
8	0.005967766887243527
9	0.0037992221242108292
10-11	0.0018868046953945915
12-13	0.0011312290594560333
14-15	0.002283802063430105
16-17	0.007077652002181522
18-19	0.0053231797627987685
20-21	0.008644297529805538
22-23	0.0185820380974042
24-25	0.02545905702111616
26-27	0.02832768316434052
28-29	0.044971691099506834
30-31	0.04281595270318496
32-33	0.04037420545032137
34-35	0.04836537827787493
36-37	0.04547967697903615
38-39	0.05532350419075545
40-41	0.05277076842639805
42-43	0.057594499887474726
44-45	0.058034185144546314
46-47	0.062977442694924
48-49	0.0658161873158231
50-51	0.03400944119504233
52-53	2.1343944518038366E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.634801352762129E-4
64-65	1.2806366710823019E-4
66-67	2.8174006763810643E-4
68-69	9.988966034441954E-4
70-71	9.818214478297648E-5
72-73	2.6039612312006805E-4
74-75	4.268788903607673E-5
76-77	8.11069891685458E-5
78-79	4.268788903607673E-6
80-81	1.7075155614430692E-5
82-83	2.0063307846956063E-4
84-85	0.001148304215070464
86-87	0.0035644387345124067
88-89	0.0023606402636950435
90-91	0.003538826001090761
92-93	0.003854716379957729
94-95	0.002676530642562011
96-97	0.0022283078076832056
98-99	0.0037650718129819674
100	0.002757637631730557
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1712924E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.317937220363824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70484971242028	28.25148828845178
2	12.851463655218371	9.59180227750453
3	4.749242781655825	5.316958319102944
4	2.233353133991893	3.33376528180849
5	1.16985162690842	2.1828224785054453
6	0.7033315099994036	1.574812868115686
7	0.46806658060630235	1.2227095489011444
8	0.3187650971790556	0.9516524707656935
9	0.2235107621227511	0.7506864530075247
>10	1.2924387419530237	8.86362319140755
>50	0.12435867818820863	3.2545923842613234
>100	0.11733851157654981	9.375273293258967
>500	0.02345812370537174	6.162411914540027
>1k	0.018278619846233943	13.119412104288127
>5k	0.0013539717025944148	3.373851908907064
>10k+	3.384929256486037E-4	2.674137217173804
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	60312	0.5149183927087719	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	58360	0.49825304082908756	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	23208	0.19814010574985375	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	17339	0.14803306159930688	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	16499	0.140861496241246	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	16407	0.1400760390829822	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	16166	0.1380184828314433	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15897	0.13572187440130237	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	15577	0.13298984950299345	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	13003	0.11101412422722115	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.537577807215346E-6	0.0	0.0	1.7075155614430692E-5	0.0
6	8.537577807215346E-6	0.0	0.0	1.7075155614430692E-5	0.0
7	8.537577807215346E-6	0.0	8.537577807215346E-6	1.7075155614430692E-5	0.0
8	8.537577807215346E-6	0.0	8.537577807215346E-6	1.7075155614430692E-5	8.537577807215346E-6
9	8.537577807215346E-6	0.0	8.537577807215346E-6	1.7075155614430692E-5	8.537577807215346E-6
10-11	8.537577807215346E-6	0.0	1.280636671082302E-5	2.561273342164604E-5	2.561273342164604E-5
12-13	1.7075155614430692E-5	0.0	1.7075155614430692E-5	5.122546684329208E-5	2.561273342164604E-5
14-15	2.561273342164604E-5	0.0	1.7075155614430692E-5	5.976304465050742E-5	2.988152232525371E-5
16-17	2.561273342164604E-5	0.0	1.7075155614430692E-5	7.683820026493811E-5	3.841910013246906E-5
18-19	3.4150311228861385E-5	0.0	1.7075155614430692E-5	1.0245093368658415E-4	5.122546684329208E-5
20-21	4.268788903607673E-5	0.0	1.7075155614430692E-5	3.030840121561448E-4	5.976304465050742E-5
22-23	4.268788903607673E-5	0.0	1.7075155614430692E-5	0.0013190557712147708	6.403183355411509E-5
24-25	4.268788903607673E-5	8.537577807215346E-6	1.7075155614430692E-5	0.004495034715498879	6.830062245772277E-5
26-27	5.122546684329208E-5	8.537577807215346E-6	1.7075155614430692E-5	0.0075813690928072264	6.830062245772277E-5
28-29	6.403183355411509E-5	8.537577807215346E-6	1.7075155614430692E-5	0.02074631407153329	6.830062245772277E-5
30-31	6.830062245772277E-5	8.537577807215346E-6	1.7075155614430692E-5	0.06361349224156154	6.830062245772277E-5
32-33	6.830062245772277E-5	8.537577807215346E-6	1.7075155614430692E-5	0.1493734613150397	7.683820026493811E-5
34-35	6.830062245772277E-5	1.280636671082302E-5	1.7075155614430692E-5	0.26463076171244687	8.537577807215346E-5
36-37	8.110698916854578E-5	1.7075155614430692E-5	1.7075155614430692E-5	0.43155748299912133	9.818214478297648E-5
38-39	9.39133558793688E-5	1.7075155614430692E-5	1.7075155614430692E-5	0.6915181896510214	1.0245093368658415E-4
40-41	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	1.0327480994498044	1.1098851149379949E-4
42-43	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	1.3265688396851205	1.1952608930101485E-4
44-45	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	1.6676706858167951	1.1952608930101485E-4
46-47	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	2.040912243603732	1.237948782046225E-4
48-49	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	2.4237372324792683	1.5367640052987622E-4
50-51	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	2.8446654311084068	1.5367640052987622E-4
52-53	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	3.2837317137889737	1.579451894334839E-4
54-55	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	3.703503070625234	1.7075155614430692E-4
56-57	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	4.1740089835808725	1.9209550066234528E-4
58-59	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	4.6927607487250835	1.9636428956595295E-4
60-61	9.39133558793688E-5	2.561273342164604E-5	1.7075155614430692E-5	5.210172114153562	1.9636428956595295E-4
62-63	1.0245093368658415E-4	2.561273342164604E-5	1.7075155614430692E-5	5.73125036925024	1.9636428956595295E-4
64-65	1.0245093368658415E-4	2.561273342164604E-5	1.7075155614430692E-5	6.275981983661808	1.9636428956595295E-4
66-67	1.0245093368658415E-4	2.561273342164604E-5	1.7075155614430692E-5	6.861459188158311	2.0917065627677598E-4
68-69	1.0245093368658415E-4	2.561273342164604E-5	1.7075155614430692E-5	7.496078690513146	2.1343944518038366E-4
70-71	1.0245093368658415E-4	2.561273342164604E-5	1.7075155614430692E-5	8.223578501832677	2.1343944518038366E-4
72-73	1.0245093368658415E-4	2.561273342164604E-5	1.7075155614430692E-5	8.901714038270889	2.1343944518038366E-4
74-75	1.0245093368658415E-4	2.561273342164604E-5	1.7075155614430692E-5	9.562001768303116	2.21977022987599E-4
76-77	1.0245093368658415E-4	2.561273342164604E-5	1.7075155614430692E-5	10.26648000106549	2.3051460079481434E-4
78-79	1.0245093368658415E-4	2.561273342164604E-5	1.7075155614430692E-5	10.982629102690327	2.3478338969842202E-4
80-81	1.0671972259019183E-4	2.561273342164604E-5	1.7075155614430692E-5	11.751813637653587	2.390521786020297E-4
82-83	1.1098851149379949E-4	2.561273342164604E-5	1.7075155614430692E-5	12.57422570145593	2.390521786020297E-4
84-85	1.1098851149379949E-4	2.561273342164604E-5	1.7075155614430692E-5	13.35438102390146	2.390521786020297E-4
86-87	1.1098851149379949E-4	2.561273342164604E-5	1.7075155614430692E-5	14.146860339911708	2.390521786020297E-4
88	1.1098851149379949E-4	2.561273342164604E-5	1.7075155614430692E-5	14.766253072247373	2.5612733421646037E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAG	5270	0.0	20.246277	1
GTATAAG	4990	0.0	20.063606	1
GTACTAG	4905	0.0	19.35719	1
TAGTACT	5555	0.0	19.288612	4
CATGGGG	29075	0.0	19.024223	4
ATGGGAG	13685	0.0	18.301481	5
GTACATA	3355	0.0	17.93277	1
CTATTGA	4615	0.0	17.509935	9
TAGGCAT	6900	0.0	17.1615	5
GGGCTAT	5115	0.0	17.086405	6
ACGTTAA	1275	0.0	16.95073	8
CGTTAAC	1615	0.0	16.880505	1
CTAGTAC	6750	0.0	16.848808	3
TACATGA	4425	0.0	16.78137	2
GTGATCG	3390	0.0	16.769745	8
TCTAACG	2495	0.0	16.76498	2
GTACATG	146355	0.0	16.761377	1
CTAACGC	2420	0.0	16.700932	3
CATGGGT	7875	0.0	16.649601	4
CTGTGCG	6355	0.0	16.559998	9
>>END_MODULE