FastQCFastQC Report
Fri 27 May 2016
ERR765399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11770508
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199220.16925352754528522No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT164080.13939925107735368No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA140830.11964649274270915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125450.10657993690671634No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC122170.1037933112147751No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC119810.10178830004618322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA446750.028.0300671
GTACTAG66250.023.2015171
GTATAAG69400.023.0966721
TATCAAC550900.022.4460452
CAACGCA505250.022.284275
ATCAACG507300.022.10453
ACGTTAA15950.021.7980168
TAGTACT78850.021.5757244
AACGCAG551950.021.3003626
GTACATG700700.021.1584591
TACATGG699750.020.3176822
GTCCTAC59300.020.2134281
ACGCAGA554200.020.0762797
TAGGCAT97800.019.7478985
GTACAAG55600.019.698611
CGTTAAC22450.019.6818541
CGCAGAG539200.019.6752388
GTACATA29250.019.445271
ATAAGGT60500.019.3427893
GTGATCG52050.019.0461718
Produced by FastQC (version 0.11.5)