##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765399_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11770508
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.465225799939986	33.0	33.0	33.0	27.0	33.0
2	31.375946475717107	33.0	33.0	33.0	27.0	33.0
3	31.323080278268364	33.0	33.0	33.0	27.0	33.0
4	34.86009235965007	37.0	37.0	37.0	33.0	37.0
5	35.12840023557182	37.0	37.0	37.0	33.0	37.0
6	35.17741298846235	37.0	37.0	37.0	33.0	37.0
7	35.030739200041324	37.0	37.0	37.0	33.0	37.0
8	35.00630525037662	37.0	37.0	37.0	33.0	37.0
9	34.693635737726865	37.0	37.0	37.0	27.0	37.0
10-11	34.69397136470235	37.0	37.0	37.0	27.0	37.0
12-13	34.68013287956646	37.0	37.0	37.0	27.0	37.0
14-15	35.905811966654284	37.0	37.0	40.0	27.0	40.0
16-17	36.15794611413543	37.0	37.0	40.0	33.0	40.0
18-19	35.98976968538656	37.0	37.0	40.0	27.0	40.0
20-21	35.972273541634735	37.0	37.0	40.0	27.0	40.0
22-23	36.14796268776165	37.0	37.0	40.0	30.0	40.0
24-25	36.166970448514206	37.0	37.0	40.0	30.0	40.0
26-27	35.84985049073498	37.0	37.0	40.0	27.0	40.0
28-29	35.74768442449553	37.0	37.0	40.0	27.0	40.0
30-31	35.54907736352585	37.0	35.0	40.0	27.0	40.0
32-33	35.510934404870206	37.0	35.0	40.0	27.0	40.0
34-35	35.46552272850076	37.0	33.0	40.0	27.0	40.0
36-37	35.237957656542946	37.0	33.0	40.0	27.0	40.0
38-39	35.11717051634475	37.0	33.0	40.0	27.0	40.0
40-41	35.246053059052336	37.0	33.0	40.0	27.0	40.0
42-43	35.159996960199166	37.0	33.0	40.0	27.0	40.0
44-45	35.08663483343285	37.0	33.0	40.0	27.0	40.0
46-47	35.02954566616836	37.0	33.0	40.0	27.0	40.0
48-49	34.828647540106175	37.0	33.0	40.0	27.0	40.0
50-51	34.6649045648667	37.0	33.0	40.0	27.0	40.0
52-53	34.5427861312358	37.0	33.0	40.0	27.0	40.0
54-55	34.43118453341181	37.0	33.0	40.0	27.0	40.0
56-57	34.22825378479841	37.0	33.0	40.0	27.0	40.0
58-59	34.018233665021086	37.0	33.0	37.0	27.0	40.0
60-61	33.753428526619246	37.0	33.0	37.0	22.0	40.0
62-63	33.529999597298605	37.0	33.0	37.0	22.0	40.0
64-65	33.31584660577097	37.0	33.0	37.0	22.0	40.0
66-67	33.09255152793745	37.0	33.0	37.0	22.0	40.0
68-69	32.87940197653322	37.0	33.0	37.0	22.0	40.0
70-71	32.669615491531886	37.0	33.0	37.0	22.0	37.0
72-73	32.43336833890262	35.0	33.0	37.0	22.0	37.0
74-75	32.201210177164825	33.0	33.0	37.0	22.0	37.0
76-77	31.999803067123356	33.0	33.0	37.0	22.0	37.0
78-79	31.79605909957327	33.0	33.0	37.0	22.0	37.0
80-81	31.655608789357267	33.0	33.0	37.0	22.0	37.0
82-83	31.488377307079695	33.0	33.0	37.0	22.0	37.0
84-85	31.3218870842278	33.0	30.0	37.0	22.0	37.0
86-87	31.11600603814211	33.0	27.0	37.0	18.5	37.0
88-89	30.973288663496938	33.0	27.0	37.0	15.0	37.0
90-91	30.85974530581008	33.0	27.0	37.0	15.0	37.0
92-93	30.748482138578893	33.0	27.0	37.0	15.0	37.0
94-95	30.606000225308883	33.0	27.0	37.0	15.0	37.0
96-97	30.490073835385864	33.0	27.0	37.0	15.0	37.0
98-99	30.36023045904221	33.0	27.0	37.0	15.0	37.0
100	30.262279164161818	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	8.0
7	161.0
8	973.0
9	2042.0
10	3624.0
11	7242.0
12	14845.0
13	26421.0
14	36283.0
15	41557.0
16	48156.0
17	57595.0
18	68976.0
19	82990.0
20	98232.0
21	116061.0
22	131838.0
23	137650.0
24	137360.0
25	143746.0
26	161193.0
27	190729.0
28	233041.0
29	288440.0
30	356272.0
31	442782.0
32	566151.0
33	740324.0
34	966143.0
35	1291680.0
36	1869099.0
37	2543799.0
38	964016.0
39	1079.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33429575099177	20.044801380659077	13.258974895994974	24.361927972354177
2	15.515908534879058	21.36962970686568	38.534691256396655	24.579770501858608
3	17.389009989466345	27.24750246346107	30.16091269915455	25.20257484791803
4	12.406914131988568	17.99690140074294	37.93952836776797	31.656656099500523
5	13.360108776263635	38.28069503423645	33.74009155097604	14.619104638523872
6	29.849198514980387	38.31453774651468	17.713561647281328	14.122702091223605
7	25.98658872749311	33.130484082507635	22.696432472313433	18.18649471768583
8	26.490855707755806	33.46969077896899	20.165939933903726	19.873513579371476
9	26.754574444892476	14.445871228205935	20.498447671728492	38.3011066551731
10-11	24.794644711466514	25.856544600521747	27.17204526675532	22.176765421256427
12-13	26.015567227804603	23.99033318244595	26.805559520314066	23.188540069435383
14-15	23.69639080130769	24.43549360370729	25.25567392919238	26.61244166579264
16-17	22.761985829920214	27.03302309068696	25.842300352214977	24.362690727177853
18-19	22.986009356436174	26.348694199237272	28.337119555991546	22.32817688833501
20-21	24.851832095690714	25.53955939027741	27.162604811894692	22.446003702137183
22-23	24.66978500272173	25.297725012792903	26.853042281081574	23.17944770340379
24-25	23.73472291646236	25.744697539707374	27.167090065810285	23.353489478019984
26-27	23.685837947902115	25.85612998296944	27.113527740102043	23.344504329026403
28-29	23.194240210166438	25.849080651447625	27.59508026734717	23.361598871038762
30-31	23.603146929300333	25.709668679978964	27.50459555796374	23.182588832756963
32-33	23.6402486584095	25.962392884604647	26.81732296568185	23.580035491304002
34-35	23.72009029844241	25.963471180109792	27.37439178026965	22.94204674117814
36-37	23.74503739719135	25.597351459566315	27.099915627730663	23.557695515511675
38-39	23.79027332550207	25.417200110949047	27.310542309778533	23.48198425377035
40-41	23.6127147214532	25.31269516220027	27.571402111124264	23.50318800522226
42-43	23.78663120140076	25.555299823672513	27.00838963508052	23.64967933984621
44-45	23.5118101019208	26.02482600025018	26.793689632875395	23.669674264953624
46-47	23.403406372449506	25.917362831900537	26.67363171949497	24.005599076154986
48-49	24.087992793513347	25.81202196495781	26.397914566815068	23.702070674713774
50-51	23.41689367160309	26.172011560838566	26.023585283493595	24.387509484064754
52-53	23.254838413556236	26.229546845836033	25.853563383161305	24.66205135744643
54-55	23.289852910341676	26.201350018198028	25.86664908600376	24.642147985456532
56-57	23.80346710609262	25.77115618119456	25.674754224711453	24.750622488001365
58-59	23.098026015529662	25.85444060698145	26.507296881324066	24.540236496164823
60-61	23.433117754985595	26.009370198805353	25.991516254013845	24.565995792195206
62-63	23.393662701876448	25.66182731285707	25.732576567642912	25.211933417623566
64-65	22.76904612226521	25.73637886590329	26.054347881032786	25.440227130798714
66-67	23.74068154040617	25.462683937255097	25.88520374830062	24.911430774038113
68-69	23.17800412460683	25.193010665627668	26.209855217362566	25.419129992402933
70-71	22.860742777345294	25.296387179507	26.05139765102567	25.791472392122035
72-73	23.45023810631302	25.370313141501693	26.123838221516504	25.055610530668787
74-75	23.501071725788407	25.404512288622538	26.097583862505775	24.996832123083284
76-77	23.019387419907243	25.42475385590247	26.082190181745567	25.473668542444717
78-79	23.0363729006587	25.63041143298197	25.987991596793936	25.345224069565397
80-81	22.83063337095138	25.448924083833795	26.40812689108839	25.312315654126436
82-83	23.11770895596283	24.99231020936153	26.6041311071816	25.285849727494043
84-85	23.214835901717194	25.310943664794035	25.89622839681047	25.5779920366783
86-87	22.730134201824104	25.216519817996545	26.370012089638262	25.68333389054109
88-89	23.085349867584558	24.496954287680033	26.416028746610177	26.001667098125232
90-91	23.15710517291946	24.679370956028162	26.261097292537595	25.90242657851478
92-93	23.181205838982443	24.58342806573596	26.3193759793971	25.91599011588449
94-95	22.405302880656127	24.621917971856046	26.87521914581919	26.09756000166863
96-97	22.891459808687927	24.60567129188111	26.356233071114467	26.146635828316494
98-99	22.531389952318055	24.785482645939112	26.27005756217614	26.41306983956669
100	22.66375794797866	24.590618405181726	26.149980828590607	26.595642818249004
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1518.0
1	1375.5
2	1524.0
3	1772.5
4	1489.5
5	1338.0
6	1649.0
7	2938.0
8	4899.5
9	5074.5
10	3366.0
11	2213.0
12	2019.0
13	2016.5
14	2240.0
15	2751.5
16	3315.0
17	4089.0
18	5095.5
19	6389.5
20	8025.0
21	10021.0
22	12836.0
23	16536.0
24	20717.5
25	25864.5
26	33174.0
27	41068.0
28	50322.0
29	65386.0
30	82658.0
31	99597.5
32	118085.0
33	135857.0
34	155718.0
35	173339.5
36	190167.5
37	216405.0
38	236746.5
39	255842.0
40	267775.5
41	273130.5
42	287742.0
43	316230.0
44	358235.0
45	414243.0
46	473085.0
47	517780.0
48	547203.5
49	555467.5
50	564524.0
51	567052.0
52	549278.0
53	535806.0
54	516811.5
55	476010.5
56	436348.5
57	383008.5
58	333428.5
59	301313.5
60	252270.0
61	197552.0
62	153310.0
63	119571.0
64	89742.0
65	64067.0
66	46628.5
67	33959.5
68	25797.0
69	20931.5
70	17641.5
71	15095.5
72	12430.5
73	11554.0
74	9288.5
75	6717.0
76	4935.0
77	3354.0
78	2401.0
79	1513.0
80	935.0
81	631.0
82	385.5
83	222.5
84	135.5
85	90.5
86	61.5
87	39.0
88	25.0
89	19.0
90	15.0
91	10.5
92	7.0
93	4.5
94	2.0
95	1.5
96	1.5
97	0.5
98	2.0
99	3.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.048358150727224346
2	0.02405163821306608
3	0.021341474811452488
4	0.022174064195020302
5	0.014986608904220617
6	0.02423005022383061
7	0.04908879039035529
8	0.0060065376957392155
9	0.0037891312762371857
10-11	0.002009259073610077
12-13	0.0011894134050968743
14-15	0.0024765286256124204
16-17	0.0072171906259271045
18-19	0.005454310043372809
20-21	0.009260432939682808
22-23	0.019251505542496553
24-25	0.025878237370893422
26-27	0.02912363680480061
28-29	0.045507806460010056
30-31	0.04307800478959786
32-33	0.040856350465077636
34-35	0.04878718913406286
36-37	0.045762680761102244
38-39	0.055639059928424495
40-41	0.05314129177772107
42-43	0.0584979000056752
44-45	0.058735782686694576
46-47	0.06368459203290121
48-49	0.06602943560294934
50-51	0.034497236652827556
52-53	3.6956773658367167E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.607234624028122E-4
64-65	1.8265991578273426E-4
66-67	4.5877374196593725E-4
68-69	0.0014782709463346867
70-71	7.64622903276562E-5
72-73	2.591222061103905E-4
74-75	5.5222765236640596E-5
76-77	1.2318924552789056E-4
78-79	4.2479050182031225E-6
80-81	3.82311451638281E-5
82-83	2.6761801614679675E-4
84-85	0.0014315439911344525
86-87	0.004345606833621794
88-89	0.003016012562924217
90-91	0.004417821218931248
92-93	0.0043498547386399975
94-95	0.0032029203837251542
96-97	0.0026761801614679673
98-99	0.00458348951464117
100	0.0032029203837251542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1770508E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.25339630363966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61221579957295	25.950806163713906
2	13.275370842472709	9.360038187749463
3	5.133636774301257	5.429343950501417
4	2.520950287935317	3.55488238249433
5	1.3255360363831328	2.3364823602685156
6	0.837299239996008	1.771058515938934
7	0.5807173488423889	1.4330581187397788
8	0.3986274303163716	1.1242376622755643
9	0.29639998574374604	0.9404195545635683
>10	1.651912858375069	10.796334192033246
>50	0.16369187394528992	4.046449866203846
>100	0.15730438934690474	11.587888539810153
>500	0.024978102692496906	6.078038224562238
>1k	0.02057078029330811	13.240531798062127
>5k	6.356853089896684E-4	1.5691617939039018
>10k+	1.525644741575204E-4	0.7812686891790876
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19922	0.16925352754528522	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	16408	0.13939925107735368	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	14083	0.11964649274270915	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12545	0.10657993690671634	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	12217	0.1037933112147751	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	11981	0.10178830004618322	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.495810036406245E-6	0.0	0.0	0.0
3	0.0	8.495810036406245E-6	0.0	0.0	0.0
4	0.0	8.495810036406245E-6	0.0	0.0	8.495810036406245E-6
5	8.495810036406245E-6	8.495810036406245E-6	0.0	1.699162007281249E-5	8.495810036406245E-6
6	8.495810036406245E-6	8.495810036406245E-6	0.0	1.699162007281249E-5	1.699162007281249E-5
7	8.495810036406245E-6	8.495810036406245E-6	0.0	1.699162007281249E-5	2.5487430109218737E-5
8	1.699162007281249E-5	8.495810036406245E-6	0.0	1.699162007281249E-5	2.5487430109218737E-5
9	1.699162007281249E-5	8.495810036406245E-6	0.0	1.699162007281249E-5	2.5487430109218737E-5
10-11	2.973533512742186E-5	8.495810036406245E-6	0.0	2.5487430109218737E-5	2.5487430109218737E-5
12-13	4.672695520023435E-5	8.495810036406245E-6	0.0	4.247905018203123E-5	2.973533512742186E-5
14-15	5.0974860218437474E-5	8.495810036406245E-6	0.0	5.0974860218437474E-5	5.0974860218437474E-5
16-17	7.221438530945308E-5	8.495810036406245E-6	0.0	6.796648029124996E-5	5.0974860218437474E-5
18-19	1.0194972043687495E-4	1.2743715054609368E-5	0.0	1.0194972043687495E-4	5.0974860218437474E-5
20-21	1.1469343549148431E-4	1.699162007281249E-5	0.0	1.7841201076453117E-4	5.0974860218437474E-5
22-23	1.5717248567351553E-4	1.699162007281249E-5	0.0	0.0010407367294597652	5.0974860218437474E-5
24-25	1.7416410574632802E-4	1.699162007281249E-5	0.0	0.0032623910539799983	5.5222765236640596E-5
26-27	1.911557258191405E-4	1.699162007281249E-5	0.0	0.005156956692098591	5.947067025484372E-5
28-29	1.9965153585554677E-4	1.699162007281249E-5	0.0	0.01382268292923296	5.947067025484372E-5
30-31	2.633701111285936E-4	1.699162007281249E-5	0.0	0.04701156483645395	5.947067025484372E-5
32-33	2.931054462560154E-4	1.699162007281249E-5	0.0	0.11681738800058587	5.947067025484372E-5
34-35	2.9735335127421857E-4	2.1239525091015615E-5	0.0	0.21281154560194004	5.947067025484372E-5
36-37	3.3133659141984355E-4	2.5487430109218737E-5	0.0	0.3654217812859054	5.947067025484372E-5
38-39	3.653198315654685E-4	3.398324014562498E-5	0.0	0.6108402458075726	5.947067025484372E-5
40-41	3.82311451638281E-4	3.398324014562498E-5	0.0	0.8903651397203927	5.947067025484372E-5
42-43	4.2054259680210913E-4	3.398324014562498E-5	0.0	1.157617836035624	5.947067025484372E-5
44-45	4.672695520023435E-4	3.398324014562498E-5	0.0	1.471291638389779	6.796648029124996E-5
46-47	4.7576536203874974E-4	3.398324014562498E-5	0.0	1.8153209700040134	6.796648029124996E-5
48-49	4.7576536203874974E-4	3.398324014562498E-5	0.0	2.1917830564322287	7.64622903276562E-5
50-51	4.7576536203874974E-4	3.398324014562498E-5	0.0	2.6019522691798858	7.64622903276562E-5
52-53	4.927569821115622E-4	3.398324014562498E-5	0.0	2.9827089875815043	8.920600538226558E-5
54-55	4.927569821115622E-4	3.398324014562498E-5	0.0	3.370954762530215	1.0619762545507807E-4
56-57	4.927569821115622E-4	3.398324014562498E-5	0.0	3.8196184905528288	1.1044553047328119E-4
58-59	5.055006971661716E-4	3.398324014562498E-5	0.0	4.294831624939213	1.1044553047328119E-4
60-61	5.18244412220781E-4	3.398324014562498E-5	0.0	4.77682016782963	1.1044553047328119E-4
62-63	5.18244412220781E-4	3.398324014562498E-5	0.0	5.255979605977924	1.1044553047328119E-4
64-65	5.18244412220781E-4	3.398324014562498E-5	0.0	5.757058234020146	1.1044553047328119E-4
66-67	5.394839373117966E-4	3.398324014562498E-5	0.0	6.300828307495309	1.1044553047328119E-4
68-69	5.479797473482028E-4	3.398324014562498E-5	0.0	6.873208021268072	1.1044553047328119E-4
70-71	5.52227652366406E-4	3.398324014562498E-5	0.0	7.4925568208271045	1.1044553047328119E-4
72-73	5.52227652366406E-4	3.398324014562498E-5	0.0	8.116268218839833	1.1894134050968743E-4
74-75	5.56475557384609E-4	3.398324014562498E-5	0.0	8.740608306795256	1.1894134050968743E-4
76-77	5.607234624028122E-4	3.398324014562498E-5	0.0	9.411416227744802	1.1894134050968743E-4
78-79	5.607234624028122E-4	3.398324014562498E-5	0.0	10.093621277858187	1.1894134050968743E-4
80-81	5.607234624028122E-4	3.398324014562498E-5	0.0	10.814962276904277	1.2743715054609368E-4
82-83	5.692192724392185E-4	3.398324014562498E-5	0.0	11.570596613162321	1.3593296058249992E-4
84-85	5.692192724392185E-4	3.398324014562498E-5	0.0	12.320007768568697	1.3593296058249992E-4
86-87	5.692192724392185E-4	3.82311451638281E-5	0.0	13.092000787051841	1.3593296058249992E-4
88	5.692192724392185E-4	4.2479050182031224E-5	0.0	13.686078799657585	1.4442877061890616E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	44675	0.0	28.030067	1
GTACTAG	6625	0.0	23.201517	1
GTATAAG	6940	0.0	23.096672	1
TATCAAC	55090	0.0	22.446045	2
CAACGCA	50525	0.0	22.28427	5
ATCAACG	50730	0.0	22.1045	3
ACGTTAA	1595	0.0	21.798016	8
TAGTACT	7885	0.0	21.575724	4
AACGCAG	55195	0.0	21.300362	6
GTACATG	70070	0.0	21.158459	1
TACATGG	69975	0.0	20.317682	2
GTCCTAC	5930	0.0	20.213428	1
ACGCAGA	55420	0.0	20.076279	7
TAGGCAT	9780	0.0	19.747898	5
GTACAAG	5560	0.0	19.69861	1
CGTTAAC	2245	0.0	19.681854	1
CGCAGAG	53920	0.0	19.675238	8
GTACATA	2925	0.0	19.44527	1
ATAAGGT	6050	0.0	19.342789	3
GTGATCG	5205	0.0	19.046171	8
>>END_MODULE