FastQCFastQC Report
Fri 27 May 2016
ERR765398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12011454
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1199960.9990131086544559No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1198600.9978808560562276No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG476110.396379988634182No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG374660.31191894003840004No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT346440.2884246986251623No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC341990.2847199015206652No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC341820.28457836994588664No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG335720.27949988402736253No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA304630.253616256616393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293920.2446997674053449No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC283520.23604136518359892No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC243670.20286469897815868No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT206010.17151129247133612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184880.15391975026503868No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG184700.15376989330350846No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC156740.1304921119458144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148060.12326567624535714No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA146620.12206682055311538No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC141380.11770431789523567No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA137950.1148487102394098No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG133850.1114353016712215No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC126500.10531614240873752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA35600.030.2183881
GTACAAG63800.030.1890161
GTACTAG68450.026.9714871
AGGGCTA68600.024.8544985
GTACAAA67000.023.7689861
CTATTGA73600.023.1013289
GTGATCG48550.022.8314048
GCTATTG74500.022.8224438
GTATAAG66200.022.6369781
TAGTACT81550.022.1191124
TAGGCAT99350.021.7949335
TACATGA49550.021.6155412
GGGCTAT85650.021.6065756
CTAGGCA108750.021.0358544
TCTAACG34500.020.832782
CGTTAAC20300.020.8272651
ATTTAGG85150.020.7438321
GGCTATT90450.020.2001447
CTGTGCG85850.020.1879549
TTTAGGG88650.020.1363052
Produced by FastQC (version 0.11.5)