FastQCFastQC Report
Fri 27 May 2016
ERR765397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16704550
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA374200.22401082339841538No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT345190.20664429751175578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAT61250.030.993331
ATATACT82650.024.7867574
TTATATA90900.022.486052
TATACTT109550.022.2600355
TATATAC147100.020.762923
GTACTAG42650.020.051431
CATGGGG336450.019.0209714
TATTATG98550.018.258827
GTATATA114100.018.1612571
GTACATG1139500.017.9088821
TACATGG1130850.017.6176782
TAGTACT53600.017.5323964
TACTTTA108550.017.3991017
ACATGGG1062300.017.1710223
GTATAAG59450.017.1514511
GTATAAT46300.016.8469031
GTATCAA754500.016.6095091
TATATCA95250.016.5748794
GAGTTAT45750.016.5358981
ATTATGC108200.016.500118
Produced by FastQC (version 0.11.5)