##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765397_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16704550
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.860156723766877	33.0	33.0	33.0	27.0	33.0
2	31.766821973653883	33.0	33.0	33.0	27.0	33.0
3	31.8008692841172	33.0	33.0	33.0	27.0	33.0
4	35.574837693921715	37.0	37.0	37.0	33.0	37.0
5	35.6151902326013	37.0	37.0	37.0	33.0	37.0
6	35.60933961106405	37.0	37.0	37.0	33.0	37.0
7	35.54422447776205	37.0	37.0	37.0	33.0	37.0
8	35.53882660712201	37.0	37.0	37.0	33.0	37.0
9	35.54002693876818	37.0	37.0	37.0	33.0	37.0
10-11	35.50435575935898	37.0	37.0	37.0	33.0	37.0
12-13	35.46010784486862	37.0	37.0	37.0	33.0	37.0
14-15	37.006777375026566	40.0	37.0	40.0	33.0	40.0
16-17	37.034043838355416	40.0	37.0	40.0	33.0	40.0
18-19	36.99623847993511	40.0	37.0	40.0	33.0	40.0
20-21	37.07917175260633	40.0	37.0	40.0	33.0	40.0
22-23	37.05004941767363	40.0	37.0	40.0	33.0	40.0
24-25	37.029859319766175	40.0	37.0	40.0	33.0	40.0
26-27	36.982254086461474	40.0	37.0	40.0	33.0	40.0
28-29	36.92371901667509	37.0	37.0	40.0	33.0	40.0
30-31	36.851757575031954	37.0	37.0	40.0	33.0	40.0
32-33	36.75210583344059	37.0	37.0	40.0	33.0	40.0
34-35	36.64667734240072	37.0	37.0	40.0	33.0	40.0
36-37	36.574959127902275	37.0	37.0	40.0	33.0	40.0
38-39	36.47920997572518	37.0	37.0	40.0	33.0	40.0
40-41	36.40044820123859	37.0	37.0	40.0	33.0	40.0
42-43	36.269291151213295	37.0	37.0	40.0	33.0	40.0
44-45	36.14584822698008	37.0	37.0	40.0	33.0	40.0
46-47	35.98783223732456	37.0	37.0	40.0	27.0	40.0
48-49	35.76833344807253	37.0	37.0	40.0	27.0	40.0
50-51	35.679573828687396	37.0	37.0	40.0	27.0	40.0
52-53	35.530319164539	37.0	33.0	40.0	27.0	40.0
54-55	35.37028067203247	37.0	33.0	40.0	27.0	40.0
56-57	35.21522809653657	37.0	33.0	40.0	27.0	40.0
58-59	35.057200613006636	37.0	33.0	40.0	27.0	40.0
60-61	34.867139132751255	37.0	33.0	40.0	27.0	40.0
62-63	34.63826203040489	37.0	33.0	37.0	27.0	40.0
64-65	34.444435138929215	37.0	33.0	37.0	27.0	40.0
66-67	34.24777545638763	37.0	33.0	37.0	27.0	40.0
68-69	34.04426174305803	37.0	33.0	37.0	27.0	40.0
70-71	33.82380438263826	37.0	33.0	37.0	27.0	40.0
72-73	33.59641049893592	37.0	33.0	37.0	27.0	37.0
74-75	33.37151177373829	37.0	33.0	37.0	27.0	37.0
76-77	33.17287379785747	37.0	33.0	37.0	27.0	37.0
78-79	33.00462637425133	37.0	33.0	37.0	27.0	37.0
80-81	32.81031937406276	37.0	33.0	37.0	27.0	37.0
82-83	32.66234199664163	35.0	33.0	37.0	27.0	37.0
84-85	32.49925149136014	33.0	33.0	37.0	22.0	37.0
86-87	32.36455313073384	33.0	33.0	37.0	22.0	37.0
88-89	32.22301806992706	33.0	33.0	37.0	22.0	37.0
90-91	32.07606218066335	33.0	33.0	37.0	22.0	37.0
92-93	31.95674828115693	33.0	33.0	37.0	22.0	37.0
94-95	31.81400034721079	33.0	33.0	37.0	22.0	37.0
96-97	31.713881666970977	33.0	33.0	37.0	22.0	37.0
98-99	31.581302998284897	33.0	33.0	37.0	22.0	37.0
100	31.502531406113903	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	1.0
5	0.0
6	10.0
7	229.0
8	1294.0
9	2589.0
10	3844.0
11	6646.0
12	12577.0
13	23243.0
14	33212.0
15	37231.0
16	42212.0
17	49130.0
18	58241.0
19	69353.0
20	80439.0
21	95168.0
22	111196.0
23	116805.0
24	122039.0
25	138471.0
26	163035.0
27	199311.0
28	247805.0
29	317442.0
30	409014.0
31	530939.0
32	692648.0
33	903513.0
34	1180646.0
35	1614149.0
36	2638021.0
37	4552444.0
38	2249378.0
39	2274.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.338089796321114	18.957983738692473	12.630217454901919	25.073709010084492
2	16.206431773994208	21.17935612813834	37.27859526094179	25.335616836925656
3	18.375885190746686	25.910101750164127	29.227024912560047	26.486988146529143
4	13.056204006992859	17.281660843474977	36.710323542445	32.95181160708716
5	14.154812839637254	37.27431831785357	33.8287598780561	14.74210896445307
6	31.71349123442415	37.10485466534567	17.412513357139222	13.769140743090954
7	27.314737601431943	31.47641810165494	22.790598968544497	18.418245328368616
8	26.54223549871143	32.83464684771514	20.756374760170132	19.866742893403295
9	26.62186649745129	14.800638149486216	19.97877225067422	38.59872310238827
10-11	25.201148788803053	25.35392153634788	27.718717355451062	21.72621231939801
12-13	26.214684023215234	23.290860873235136	26.990454696474913	23.504000407074717
14-15	23.89387621935341	24.262913397846695	24.527188700084707	27.316021682715185
16-17	23.70911134563235	26.72247344889075	25.57097104663114	23.99744415884576
18-19	24.349508603351282	26.00630898358926	26.70733908812747	22.936843324931992
20-21	25.478430983078937	25.404205239479044	25.768641128916148	23.348722648525868
22-23	24.485616417074723	25.10416385417126	25.79006450714514	24.620155221608872
24-25	24.110896549577134	25.776964033786108	25.718933636825987	24.393205779810774
26-27	24.559860662155298	25.651112151207876	25.539192989476383	24.249834197160443
28-29	24.35643582734637	25.21903814017549	26.092873446822296	24.331652585655846
30-31	24.497713037874096	25.411773384430607	25.977074963181284	24.11343861451401
32-33	24.118470630825286	25.5158907125078	25.590197619191386	24.775441037475535
34-35	24.640573324837895	25.307870882885908	26.07212817592601	23.979427616350186
36-37	24.65749229505099	25.40028733849783	25.563124661273225	24.37909570517796
38-39	24.73391613530328	24.966285670165135	25.700586692958133	24.599211501573457
40-41	24.311131006722494	25.3148387184473	25.957874432909456	24.41615584192075
42-43	25.00925963181333	25.07407406076857	25.809599576757286	24.10706673066081
44-45	24.473359227529368	25.382659777979867	25.36051530374169	24.78346569074908
46-47	23.946376756535205	25.17638960901093	26.229262169219243	24.647971465234622
48-49	24.53475421314784	25.63612765945345	25.579207448782213	24.249910678616498
50-51	24.046015915944523	25.76561529265055	25.350102943367887	24.838265848037043
52-53	24.02721882198858	25.493477825889464	25.461082450452068	25.018220901669885
54-55	24.11828933635936	25.396398209233222	25.67276581767289	24.812546636734528
56-57	24.07971174042868	24.85368264235346	25.430914261397714	25.635691355820146
58-59	23.795822112230013	24.905098489748614	25.82984074340779	25.469238654613584
60-61	24.060557364545236	25.01307582854919	25.64088923477686	25.285477572128713
62-63	23.37707812824223	25.247625194101442	25.592880041619075	25.782416636037258
64-65	23.531661842294643	24.83728670803485	25.633242234245834	25.997809215424677
66-67	23.86554111667936	25.002564822041467	25.434212322120576	25.6976817391586
68-69	24.043442888973253	24.794550621636024	25.380124829501877	25.78188165988885
70-71	23.458976162803072	24.477743634697692	25.712297714274236	26.350982488225
72-73	23.907669991741496	24.622805350629616	25.649203228540014	25.820321429088878
74-75	23.92199800789729	24.73248256491066	25.598406078367773	25.747113348824275
76-77	23.709356186926055	24.7487758564754	25.700667969362485	25.841199987236063
78-79	23.77064804742122	24.895688510311295	25.544780019273716	25.78888342299377
80-81	23.420365597770616	24.791506900979694	25.897286003777502	25.890841497472184
82-83	23.55970667569257	24.748385376551514	25.393477475609288	26.298430472146634
84-85	23.374687195490598	24.91437892309581	25.256282535754792	26.454651345658792
86-87	23.307951747738688	24.685969889974483	25.475730746402526	26.53034761588431
88-89	23.449250396169095	24.424259491101633	25.521287649683256	26.60520246304602
90-91	23.67150675217895	24.422319897619925	25.372523354219517	26.5336499959816
92-93	23.527561773064388	24.535630441091815	25.416513812435166	26.52029397340863
94-95	23.022698024886868	24.56463127267398	25.80379627539593	26.608874427043222
96-97	23.462275914648792	24.42723579045099	25.40428235890934	26.706205935990873
98-99	23.414033116586204	24.452807182456112	25.38879907147028	26.744360629487403
100	23.17454110484928	24.622590358578744	25.342080196970855	26.860788339601122
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	870.0
1	843.5
2	1118.5
3	1400.5
4	1144.5
5	971.5
6	1262.5
7	2369.0
8	3791.5
9	3657.0
10	2333.0
11	1672.5
12	1702.5
13	1927.5
14	2261.5
15	2673.5
16	3173.0
17	3776.0
18	4531.0
19	5489.5
20	6885.0
21	8965.0
22	11616.0
23	14811.5
24	19301.0
25	25704.0
26	35060.0
27	46132.0
28	57840.0
29	74756.0
30	94226.5
31	114108.0
32	136291.0
33	158095.5
34	181724.0
35	201788.0
36	226403.5
37	260180.5
38	282098.5
39	301504.5
40	338007.5
41	373334.0
42	393121.0
43	438556.0
44	489678.0
45	523170.5
46	595888.5
47	676226.0
48	746765.5
49	802242.0
50	861927.0
51	874908.0
52	825432.5
53	775124.0
54	710572.0
55	665285.0
56	629409.0
57	579148.0
58	539361.5
59	489071.0
60	412855.5
61	334429.0
62	274366.5
63	217003.5
64	162160.5
65	132782.5
66	105419.0
67	77515.5
68	59329.0
69	48179.0
70	42392.5
71	38118.5
72	33410.0
73	34253.5
74	27483.0
75	20036.0
76	15244.0
77	10783.0
78	7929.5
79	5047.5
80	3612.0
81	2772.5
82	2233.5
83	1897.0
84	1477.5
85	969.5
86	639.5
87	324.0
88	151.0
89	123.0
90	99.0
91	73.5
92	56.0
93	33.5
94	24.0
95	18.5
96	9.5
97	9.0
98	11.5
99	10.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01834230793406587
2	0.0058546922844374735
3	0.0065131955066134685
4	0.0039031281896249824
5	0.04604733440888859
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.130611120922144E-4
18-19	0.0016432648589755486
20-21	6.165984716738853E-4
22-23	0.004902855808746719
24-25	0.007393195267157751
26-27	0.005842719498579728
28-29	0.022439993893879212
30-31	0.020739858302079375
32-33	0.013167071247055444
34-35	0.013505302447536751
36-37	0.013756730950549401
38-39	0.021344483987895513
40-41	0.03092869906702066
42-43	0.018090879431053218
44-45	0.017159995330613517
46-47	0.018548838490111973
48-49	0.013304758284419516
50-51	0.010039180941719471
52-53	0.027749924421789272
54-55	0.025918088185554234
56-57	0.013424486142996968
58-59	0.01652843087661745
60-61	0.017549110870990238
62-63	0.02127264727274904
64-65	0.011122718061845427
66-67	0.01563346513375098
68-69	0.007704487699459129
70-71	0.008826936373622757
72-73	0.010583942698246885
74-75	0.0035439446138926223
76-77	0.007417140838873241
78-79	0.00582476031979311
80-81	0.002394557171549069
82-83	2.155101454394162E-4
84-85	0.002556189780628631
86-87	0.002271836116507179
88-89	0.0011553738352724258
90-91	7.632650984312657E-4
92-93	0.0010895235130548265
94-95	0.0031099311265493533
96-97	0.0013678907842474056
98-99	0.0017480267352308204
100	0.0021131967038920535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.670455E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.65130360902576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3798544802412	25.804361672521818
2	14.3233834893283	10.212945869731
3	5.193988172869846	5.555173478780155
4	2.4249375219613434	3.4580873531344967
5	1.351877078537349	2.4098090084508894
6	0.8649829296515066	1.8502661424978257
7	0.5703008876241412	1.4232379065229597
8	0.4139241681925594	1.18055489530771
9	0.29939728061737964	0.9606513015906197
>10	1.8416791077219268	12.176541791542029
>50	0.16780797742744422	4.1661430627733145
>100	0.13085509939882073	9.48504233068589
>500	0.018907992756284728	4.695408976906412
>1k	0.016283369633561997	11.38189945445785
>5k	0.0014833247719415966	3.486979390220802
>10k+	3.371192663503628E-4	1.7528973648763682
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	37420	0.22401082339841538	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	34519	0.20664429751175578	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.986392928872672E-6	0.0	0.0	0.0
3	0.0	5.986392928872672E-6	0.0	0.0	0.0
4	0.0	1.1972785857745345E-5	0.0	0.0	0.0
5	0.0	1.1972785857745345E-5	0.0	0.0	0.0
6	0.0	1.1972785857745345E-5	0.0	0.0	5.986392928872672E-6
7	0.0	1.1972785857745345E-5	0.0	0.0	5.986392928872672E-6
8	0.0	1.1972785857745345E-5	0.0	1.1972785857745345E-5	5.986392928872672E-6
9	0.0	1.1972785857745345E-5	0.0	1.1972785857745345E-5	2.9931964644363363E-5
10-11	5.986392928872672E-6	1.1972785857745345E-5	0.0	2.9931964644363363E-5	2.9931964644363363E-5
12-13	8.979589393309008E-6	1.1972785857745345E-5	0.0	2.9931964644363363E-5	5.687073282429039E-5
14-15	1.496598232218168E-5	3.591835757323603E-5	0.0	2.9931964644363363E-5	9.578228686196276E-5
16-17	2.394557171549069E-5	3.591835757323603E-5	0.0	4.789114343098138E-5	1.1374146564858078E-4
18-19	2.394557171549069E-5	4.1904750502108706E-5	0.0	6.585032221759939E-5	1.1972785857745345E-4
20-21	2.394557171549069E-5	4.789114343098138E-5	0.0	1.3170064443519878E-4	1.2870744797076247E-4
22-23	2.394557171549069E-5	5.387753635985405E-5	0.0	6.465304363182487E-4	1.3768703736407147E-4
24-25	3.591835757323603E-5	5.986392928872672E-5	0.0	0.002137142275607544	1.4965982322181682E-4
26-27	4.789114343098138E-5	7.48299116109084E-5	0.0	0.0038702030285161825	1.5564621615068948E-4
28-29	4.789114343098138E-5	8.081630453978108E-5	0.0	0.01790230805379373	1.736053949373075E-4
30-31	4.789114343098138E-5	8.380950100421741E-5	0.0	0.0661137235064698	1.736053949373075E-4
32-33	4.789114343098138E-5	8.380950100421741E-5	0.0	0.1582443106818202	1.9755096665279818E-4
34-35	6.285712575316306E-5	1.0476187625527177E-4	0.0	0.28147720231912865	2.0952375251054352E-4
36-37	7.48299116109084E-5	1.1972785857745345E-4	0.0	0.48234762385098673	2.0952375251054352E-4
38-39	8.380950100421741E-5	1.2571425150632612E-4	0.0	0.8041282165637506	2.1551014543941618E-4
40-41	8.380950100421741E-5	1.406802338285078E-4	0.0	1.1934652534788426	2.2149653836828887E-4
42-43	8.680269746865374E-5	1.496598232218168E-4	0.0	1.5370273368633098	2.244897348327252E-4
44-45	9.278909039752643E-5	1.6462580554399848E-4	0.0	1.9321921272946592	2.3646252069047056E-4
46-47	9.578228686196276E-5	1.7959178786618017E-4	0.0	2.390127240781703	2.4544211008377956E-4
48-49	9.578228686196276E-5	1.7959178786618017E-4	0.0	2.8672397640163907	2.6938768179927025E-4
50-51	9.578228686196276E-5	1.8557818079505286E-4	0.0	3.3653735060208145	2.6938768179927025E-4
52-53	9.877548332639909E-5	1.8557818079505286E-4	0.0	3.8650008530609923	2.8435366412145194E-4
54-55	1.0176867979083543E-4	1.8557818079505286E-4	0.0	4.355603712760894	3.053060393725063E-4
56-57	1.0176867979083543E-4	1.8557818079505286E-4	0.0	4.906860705616134	3.2625841462356064E-4
58-59	1.0176867979083543E-4	1.8557818079505286E-4	0.0	5.523255639930438	3.4122439694574233E-4
60-61	1.1972785857745345E-4	1.9455777018836186E-4	0.0	6.150716421573763	3.5619037926792397E-4
62-63	1.1972785857745345E-4	2.0353735958167086E-4	0.0	6.772870265885642	3.7714275451897834E-4
64-65	1.2571425150632612E-4	2.155101454394162E-4	0.0	7.396505742447417	3.8312914744785103E-4
66-67	1.2571425150632612E-4	2.2149653836828887E-4	0.0	8.064721887150508	3.8312914744785103E-4
68-69	1.2571425150632612E-4	2.244897348327252E-4	0.0	8.812455289127813	3.8612234391228735E-4
70-71	1.2870744797076247E-4	2.3346932422603421E-4	0.0	9.641786219922118	3.891155403767237E-4
72-73	1.3469384089963513E-4	2.3346932422603421E-4	0.0	10.435231718304294	4.280270944143961E-4
74-75	1.4666662675738047E-4	2.4244891361934324E-4	0.0	11.197616816975016	4.310202908788324E-4
76-77	1.5265301968625316E-4	2.5142850301265225E-4	0.0	11.989535785160331	4.370066838077051E-4
78-79	1.5564621615068948E-4	2.5741489594152493E-4	0.0	12.824508891290098	4.399998802721414E-4
80-81	1.5863941261512582E-4	2.6040809240596125E-4	0.0	13.714377220577628	4.489794696654504E-4
82-83	1.6462580554399848E-4	2.6938768179927025E-4	0.0	14.63404282066862	4.579590590587595E-4
84-85	1.6761900200843482E-4	2.7537407472814294E-4	0.0	15.508050201891102	4.609522555231958E-4
86-87	1.7061219847287117E-4	2.7537407472814294E-4	0.0	16.393314995016325	4.669386484520685E-4
88	1.736053949373075E-4	2.9333325351476095E-4	0.0	17.06943916477846	4.789114343098138E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATAT	6125	0.0	30.99333	1
ATATACT	8265	0.0	24.786757	4
TTATATA	9090	0.0	22.48605	2
TATACTT	10955	0.0	22.260035	5
TATATAC	14710	0.0	20.76292	3
GTACTAG	4265	0.0	20.05143	1
CATGGGG	33645	0.0	19.020971	4
TATTATG	9855	0.0	18.25882	7
GTATATA	11410	0.0	18.161257	1
GTACATG	113950	0.0	17.908882	1
TACATGG	113085	0.0	17.617678	2
TAGTACT	5360	0.0	17.532396	4
TACTTTA	10855	0.0	17.399101	7
ACATGGG	106230	0.0	17.171022	3
GTATAAG	5945	0.0	17.151451	1
GTATAAT	4630	0.0	16.846903	1
GTATCAA	75450	0.0	16.609509	1
TATATCA	9525	0.0	16.574879	4
GAGTTAT	4575	0.0	16.535898	1
ATTATGC	10820	0.0	16.50011	8
>>END_MODULE