##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765396_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8026788 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23306856989371 33.0 31.0 34.0 31.0 34.0 2 32.23906025673034 34.0 31.0 34.0 31.0 34.0 3 32.2500753726148 34.0 31.0 34.0 30.0 34.0 4 35.722308101322724 37.0 35.0 37.0 35.0 37.0 5 35.75617544651734 37.0 35.0 37.0 35.0 37.0 6 35.85882559748681 37.0 37.0 37.0 35.0 37.0 7 35.818413542253765 37.0 36.0 37.0 35.0 37.0 8 35.85135860072547 37.0 37.0 37.0 35.0 37.0 9 37.53730184477278 39.0 38.0 39.0 35.0 39.0 10-11 37.47434534212191 39.0 38.0 39.0 35.0 39.0 12-13 37.452638651973864 39.0 38.0 39.0 35.0 39.0 14-15 38.805035650623886 41.0 38.5 41.0 35.0 41.0 16-17 38.76773424189103 40.5 38.5 41.0 35.0 41.0 18-19 38.76808294924446 41.0 38.5 41.0 35.0 41.0 20-21 38.75483400084816 41.0 39.0 41.0 35.0 41.0 22-23 38.64764710367335 40.5 38.5 41.0 34.5 41.0 24-25 38.55651812406158 40.0 38.0 41.0 34.0 41.0 26-27 38.4197825456459 40.0 38.0 41.0 34.0 41.0 28-29 38.268003216230454 40.0 38.0 41.0 34.0 41.0 30-31 38.02137548668284 40.0 38.0 41.0 34.0 41.0 32-33 37.730843645054534 40.0 37.0 41.0 33.0 41.0 34-35 37.56883743534774 40.0 37.0 41.0 33.0 41.0 36-37 37.38996538839695 40.0 37.0 41.0 33.0 41.0 38-39 37.174215452058775 40.0 36.5 41.0 32.0 41.0 40-41 36.89259963013848 40.0 36.0 41.0 31.0 41.0 42-43 36.593142674255255 40.0 36.0 41.0 30.5 41.0 44-45 36.18846623331773 39.5 35.0 41.0 30.0 41.0 46-47 35.69868127823982 39.0 35.0 41.0 28.5 41.0 48-49 35.24817349106517 39.0 35.0 41.0 26.0 41.0 50-51 33.95966462550151 38.0 33.5 40.0 24.0 40.5 52-53 33.87022760536344 38.0 33.5 39.5 23.0 40.0 54-55 34.646313195265655 38.5 35.0 40.5 24.0 41.0 56-57 34.646987312982475 38.5 35.0 41.0 24.0 41.0 58-59 34.428944429577555 38.0 35.0 41.0 23.0 41.0 60-61 34.08036514730425 37.0 35.0 40.0 22.5 41.0 62-63 33.6485173770629 37.0 34.0 40.0 21.0 41.0 64-65 33.35084450965941 36.0 34.0 39.0 20.0 41.0 66-67 33.06239974196403 36.0 34.0 39.0 20.0 41.0 68-69 32.82977275592678 35.0 34.0 39.0 19.5 41.0 70-71 32.481986194228625 35.0 34.0 37.5 17.5 40.5 72-73 32.129393849694296 35.0 34.0 37.0 14.5 39.5 74-75 31.76085976108999 35.0 33.0 37.0 9.0 39.0 76-77 31.40882517390518 35.0 33.0 36.0 6.0 38.5 78-79 31.11346512453051 35.0 33.0 36.0 2.0 37.0 80-81 30.81255447384433 35.0 33.0 35.0 2.0 37.0 82-83 30.55246033656302 35.0 33.0 35.0 2.0 36.5 84-85 30.296599523993905 35.0 32.5 35.0 2.0 36.0 86-87 30.040315441244992 35.0 32.0 35.0 2.0 36.0 88-89 29.82058925687336 35.0 32.0 35.0 2.0 36.0 90-91 29.58023284033414 35.0 32.0 35.0 2.0 35.0 92-93 29.303801034735187 35.0 31.0 35.0 2.0 35.0 94-95 29.01034025067063 35.0 31.0 35.0 2.0 35.0 96-97 28.73955977160478 35.0 31.0 35.0 2.0 35.0 98-99 28.4363675731812 35.0 30.5 35.0 2.0 35.0 100 27.91160337111183 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43778369685513496 1101 2 0.5424642509600304 1101 3 0.5964719995737937 1101 4 0.4016892985849623 1101 5 0.43788909942445287 1101 6 0.4345138839264422 1101 7 0.4804467223795328 1101 8 0.4681356064453439 1101 9 0.6291710677278175 1101 10-11 0.6186891939551629 1101 12-13 0.599110591106907 1101 14-15 0.773820094252244 1101 16-17 0.793174194784406 1101 18-19 0.7762274534291507 1101 20-21 0.7641678940561505 1101 22-23 0.7224260229003434 1101 24-25 0.7251061302625104 1101 26-27 0.7363955982016748 1101 28-29 0.7710659639626911 1101 30-31 0.8182834796348999 1101 32-33 0.8074830720651249 1101 34-35 0.9733305410464013 1101 36-37 1.0472398148825022 1101 38-39 1.0266567589187972 1101 40-41 1.0889282640322833 1101 42-43 1.0710861701112933 1101 44-45 1.1741680797913787 1101 46-47 1.1558198761335063 1101 48-49 1.2647014532936538 1101 50-51 1.2096808621817203 1101 52-53 1.2582482300382836 1101 54-55 1.45249163033197 1101 56-57 1.5050359102918662 1101 58-59 1.5071047324393305 1101 60-61 1.3965849665599137 1101 62-63 1.4884881651333828 1101 64-65 1.4406909945484898 1101 66-67 1.3991012940278864 1101 68-69 1.3661818801068435 1101 70-71 1.3348385026938843 1101 72-73 1.295591350178519 1101 74-75 1.2925033184029644 1101 76-77 1.2086082863257595 1101 78-79 1.2827119038338033 1101 80-81 1.354407650310506 1101 82-83 1.359540311285322 1101 84-85 1.3415216779177754 1101 86-87 1.3562599266705355 1101 88-89 1.389303030295384 1101 90-91 1.3415064087861701 1101 92-93 1.2781540520092527 1101 94-95 1.2531001205213919 1101 96-97 1.2854700813923081 1101 98-99 1.2791039758030287 1101 100 1.3753836700683806 1102 1 0.4077148846682306 1102 2 0.5056244037866904 1102 3 0.5472005073823993 1102 4 0.3865131798860588 1102 5 0.42665775347644797 1102 6 0.4138050649361844 1102 7 0.47154200843793603 1102 8 0.4499776861184941 1102 9 0.5406315484143889 1102 10-11 0.5550505816717788 1102 12-13 0.58525007444198 1102 14-15 0.7573385761571529 1102 16-17 0.7574062943879838 1102 18-19 0.7425994516498591 1102 20-21 0.7573346146066129 1102 22-23 0.7958899308598717 1102 24-25 0.7858820833270315 1102 26-27 0.8026512169784965 1102 28-29 0.8383236540065511 1102 30-31 0.9033108036863453 1102 32-33 0.899501930080163 1102 34-35 0.8495914070649491 1102 36-37 0.911553762845223 1102 38-39 0.928462530119198 1102 40-41 0.9896734752215011 1102 42-43 1.0408920556577854 1102 44-45 1.0885997767196969 1102 46-47 1.0186460929176633 1102 48-49 1.0982626056678413 1102 50-51 1.1729305901014087 1102 52-53 1.181617330016124 1102 54-55 1.3075897496856115 1102 56-57 1.2797912114473036 1102 58-59 1.348808116043088 1102 60-61 1.318107508707989 1102 62-63 1.3514405094854496 1102 64-65 1.3201626773246744 1102 66-67 1.297503426989195 1102 68-69 1.246763294106323 1102 70-71 1.1336699837042374 1102 72-73 1.1476995225392344 1102 74-75 1.1678447030887043 1102 76-77 1.128562098159211 1102 78-79 1.155577540117779 1102 80-81 1.152208916083481 1102 82-83 1.106614085925198 1102 84-85 1.1165812999560956 1102 86-87 1.1701757026397495 1102 88-89 1.160951191673604 1102 90-91 1.1527218706744087 1102 92-93 1.1915845268936032 1102 94-95 1.0969387858393453 1102 96-97 1.1388308458270409 1102 98-99 1.2053907072426924 1102 100 1.2265566325280552 1103 1 0.3892039820871247 1103 2 0.4870046249681934 1103 3 0.5179293642511098 1103 4 0.4384407380088291 1103 5 0.47502900782804147 1103 6 0.4441310819590072 1103 7 0.4938414558190374 1103 8 0.4871844268457082 1103 9 0.5691060219932069 1103 10-11 0.5837267341369099 1103 12-13 0.6264812287176227 1103 14-15 0.7354035639077452 1103 16-17 0.7703207529826486 1103 18-19 0.7688903059119667 1103 20-21 0.7607316559054667 1103 22-23 0.7753965855487337 1103 24-25 0.780695638630668 1103 26-27 0.7824812968240522 1103 28-29 0.8206923785539075 1103 30-31 0.8592712921269765 1103 32-33 0.9084944777394739 1103 34-35 0.9063271112823443 1103 36-37 0.8391962719502359 1103 38-39 0.8502005069634109 1103 40-41 0.853947402209009 1103 42-43 0.8872555960263853 1103 44-45 0.9190579431965276 1103 46-47 0.9225751030212592 1103 48-49 0.9273539366904515 1103 50-51 0.9107014802969218 1103 52-53 1.0053305834488384 1103 54-55 0.9994948784551454 1103 56-57 1.0458907520552074 1103 58-59 1.0290157577842294 1103 60-61 1.0262978838432133 1103 62-63 1.0808096791819182 1103 64-65 1.125588205487169 1103 66-67 1.0005845999503862 1103 68-69 1.01764065307286 1103 70-71 0.9930676304415869 1103 72-73 0.9482608144806974 1103 74-75 0.9959222594924881 1103 76-77 0.9138519518404458 1103 78-79 0.9350728874836598 1103 80-81 0.8883022036189914 1103 82-83 0.9315212386274183 1103 84-85 0.9297003730994078 1103 86-87 0.8964790139329182 1103 88-89 0.912772083677396 1103 90-91 0.9505996323736419 1103 92-93 1.0064214648035623 1103 94-95 0.9425123539839966 1103 96-97 0.9412038432699994 1103 98-99 0.9731493212350166 1103 100 1.0593965141371484 1104 1 0.3072232552653986 1104 2 0.3822189125099342 1104 3 0.40771725217088317 1104 4 0.3500500112532521 1104 5 0.42144042486046374 1104 6 0.37415719706136485 1104 7 0.4267116909545834 1104 8 0.34821643167436633 1104 9 0.4666798225025488 1104 10-11 0.48991211836708715 1104 12-13 0.48072400233181867 1104 14-15 0.5546094917633937 1104 16-17 0.5700209625107959 1104 18-19 0.5656196694936355 1104 20-21 0.5255606201570444 1104 22-23 0.5221340607843814 1104 24-25 0.5471206818868453 1104 26-27 0.562628753980924 1104 28-29 0.5511546699034824 1104 30-31 0.4741253983215401 1104 32-33 0.5413928994280042 1104 34-35 0.587873857642343 1104 36-37 0.5778671944822165 1104 38-39 0.54123845635322 1104 40-41 0.5362679537329385 1104 42-43 0.6453007288626438 1104 44-45 0.678133042885591 1104 46-47 0.5939104904833385 1104 48-49 0.5890693827381597 1104 50-51 0.47488922138997935 1104 52-53 0.5346806038485212 1104 54-55 0.6320275783236227 1104 56-57 0.5754145523373992 1104 58-59 0.6070236172616035 1104 60-61 0.6324049430902932 1104 62-63 0.6174291642235943 1104 64-65 0.5651366571475691 1104 66-67 0.5509220854037977 1104 68-69 0.5641835143670448 1104 70-71 0.5349422757186204 1104 72-73 0.6126860829742427 1104 74-75 0.5695217707767881 1104 76-77 0.4807924846296636 1104 78-79 0.4921515339839537 1104 80-81 0.5347683080636187 1104 82-83 0.5485502583833473 1104 84-85 0.520979982429715 1104 86-87 0.5105429760879368 1104 88-89 0.5806476943030212 1104 90-91 0.5747482972523805 1104 92-93 0.5833710832031436 1104 94-95 0.5571557982731754 1104 96-97 0.56477009697225 1104 98-99 0.5535889533536782 1104 100 0.5940147060962992 1105 1 0.19166716946195095 1105 2 0.29619047996992265 1105 3 0.317938274722124 1105 4 0.2544379068327558 1105 5 0.29596728501063296 1105 6 0.2698493759660394 1105 7 0.30389375096073223 1105 8 0.31989980958941544 1105 9 0.3808275402763357 1105 10-11 0.36192680829404367 1105 12-13 0.3753297069986914 1105 14-15 0.485563623783662 1105 16-17 0.4629606657181071 1105 18-19 0.4333946804488633 1105 20-21 0.4199488962454794 1105 22-23 0.34308727879867007 1105 24-25 0.35033108420923753 1105 26-27 0.32573380062054724 1105 28-29 0.34139600314400553 1105 30-31 0.35700985890752435 1105 32-33 0.3821271254607126 1105 34-35 0.37079286358898145 1105 36-37 0.4298170994655308 1105 38-39 0.3935922464218464 1105 40-41 0.38170225849213324 1105 42-43 0.5021391928732655 1105 44-45 0.5275816722546338 1105 46-47 0.5412579153187451 1105 48-49 0.5229359325870462 1105 50-51 0.4928189215275509 1105 52-53 0.5465807530993132 1105 54-55 0.5897743799089383 1105 56-57 0.5362017611179084 1105 58-59 0.5351655493739713 1105 60-61 0.4877900912312967 1105 62-63 0.5089854749802072 1105 64-65 0.3892001344528282 1105 66-67 0.36182499100848986 1105 68-69 0.40187926784462036 1105 70-71 0.3605235717541433 1105 72-73 0.4097827107022809 1105 74-75 0.40720063850204724 1105 76-77 0.4156287349128185 1105 78-79 0.4032108084308099 1105 80-81 0.3562405177884429 1105 82-83 0.39545389105607853 1105 84-85 0.35919772318951715 1105 86-87 0.3003574225319632 1105 88-89 0.32134518713789717 1105 90-91 0.38160041429421554 1105 92-93 0.3986538266561048 1105 94-95 0.36283944621760966 1105 96-97 0.31850673386959016 1105 98-99 0.3313595790059267 1105 100 0.42355378532603183 1106 1 0.1984816317833733 1106 2 0.2840371459598572 1106 3 0.34775246846595564 1106 4 0.28626702725173914 1106 5 0.3331992162949504 1106 6 0.30190847916009744 1106 7 0.281860475889701 1106 8 0.3105618150074889 1106 9 0.2955410385734041 1106 10-11 0.3866828844653867 1106 12-13 0.3782905865321311 1106 14-15 0.4064481117626926 1106 16-17 0.4500980033687725 1106 18-19 0.3829299004604323 1106 20-21 0.41584329066646575 1106 22-23 0.3958205447761287 1106 24-25 0.43079268937798076 1106 26-27 0.4178820994617567 1106 28-29 0.41028912776928905 1106 30-31 0.41442087870401423 1106 32-33 0.436483554351625 1106 34-35 0.3729508190632558 1106 36-37 0.41555755610558265 1106 38-39 0.3894737869204903 1106 40-41 0.4586942298463228 1106 42-43 0.5024620113676903 1106 44-45 0.5323783992032176 1106 46-47 0.4439230918556376 1106 48-49 0.4081664652060084 1106 50-51 0.45965317492415636 1106 52-53 0.5279499839681918 1106 54-55 0.5559818806781962 1106 56-57 0.5566597367883759 1106 58-59 0.573738435541479 1106 60-61 0.5360477304924771 1106 62-63 0.5786390627643669 1106 64-65 0.5863390562089918 1106 66-67 0.522205577475134 1106 68-69 0.5116419176902909 1106 70-71 0.5508937468741237 1106 72-73 0.529812373193316 1106 74-75 0.5414832521743378 1106 76-77 0.6094171281579897 1106 78-79 0.5405547869266307 1106 80-81 0.5607459431349753 1106 82-83 0.5728247524235179 1106 84-85 0.560832593415789 1106 86-87 0.6041424301399054 1106 88-89 0.5973913477208619 1106 90-91 0.5790271119107508 1106 92-93 0.645342669477948 1106 94-95 0.6462681695330019 1106 96-97 0.6714192344115055 1106 98-99 0.7278914519750721 1106 100 0.8491943732677463 1107 1 0.2027678264619368 1107 2 0.25353752236481597 1107 3 0.2706567535454738 1107 4 0.2409940625832263 1107 5 0.3190416464087278 1107 6 0.23869760740998203 1107 7 0.3041720625500943 1107 8 0.21313118527170616 1107 9 0.28504952578670384 1107 10-11 0.32937638747858955 1107 12-13 0.3417529106179771 1107 14-15 0.37875829269623296 1107 16-17 0.36636274210069786 1107 18-19 0.4312730746670539 1107 20-21 0.3824982638846208 1107 22-23 0.40723510964555487 1107 24-25 0.4000602569977616 1107 26-27 0.36156111123735 1107 28-29 0.4113340932802956 1107 30-31 0.45248772627751777 1107 32-33 0.4201749396648111 1107 34-35 0.45116501351187566 1107 36-37 0.502684961920707 1107 38-39 0.49080897612740415 1107 40-41 0.49660402867345965 1107 42-43 0.4908132162797898 1107 44-45 0.4649951102641552 1107 46-47 0.4060363029708327 1107 48-49 0.4307646081073244 1107 50-51 0.31507644556207737 1107 52-53 0.3888784343561582 1107 54-55 0.4607138164206148 1107 56-57 0.4865578663549215 1107 58-59 0.4061684283809086 1107 60-61 0.4692539201512602 1107 62-63 0.5101395481720559 1107 64-65 0.5461411188736278 1107 66-67 0.46441709680199494 1107 68-69 0.43684852419813325 1107 70-71 0.47192002014460144 1107 72-73 0.4631783916392749 1107 74-75 0.44986918246630125 1107 76-77 0.4297637992475849 1107 78-79 0.5178831064484974 1107 80-81 0.5165408989472482 1107 82-83 0.538221340952191 1107 84-85 0.5540105617336906 1107 86-87 0.5581579320872656 1107 88-89 0.5548834320651004 1107 90-91 0.5461950811914598 1107 92-93 0.5920877599965486 1107 94-95 0.5023233183178029 1107 96-97 0.5688013469378177 1107 98-99 0.6005146780695121 1107 100 0.622688019146775 1108 1 0.14030253627426248 1108 2 0.14214804244976165 1108 3 0.18846599650953522 1108 4 0.21740480148221764 1108 5 0.2695197337871704 1108 6 0.23182233455151646 1108 7 0.28252573327155517 1108 8 0.24225876339968266 1108 9 0.33297075028339407 1108 10-11 0.3171121710035365 1108 12-13 0.32878199980392964 1108 14-15 0.3568007452506734 1108 16-17 0.3630129782271396 1108 18-19 0.40597207543864755 1108 20-21 0.3353587299232359 1108 22-23 0.328439615500173 1108 24-25 0.37627059847957156 1108 26-27 0.407234765296721 1108 28-29 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-0.8985488573122851 2316 72-73 -0.8476083147292002 2316 74-75 -0.7847026777244182 2316 76-77 -0.7732317106285045 2316 78-79 -0.7709821995451307 2316 80-81 -0.7240065647821652 2316 82-83 -0.6937602008633768 2316 84-85 -0.7247980927100635 2316 86-87 -0.7164553880067359 2316 88-89 -0.6166026423600108 2316 90-91 -0.6029455862941724 2316 92-93 -0.5475016224809188 2316 94-95 -0.43654805705121547 2316 96-97 -0.49207385092381983 2316 98-99 -0.5774088490144713 2316 100 -0.7089771868235495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22042.0 3 4022.0 4 3924.0 5 4492.0 6 5632.0 7 6514.0 8 8071.0 9 9962.0 10 13038.0 11 19234.0 12 29393.0 13 41585.0 14 43894.0 15 53283.0 16 71336.0 17 99015.0 18 98065.0 19 55912.0 20 45568.0 21 41436.0 22 42232.0 23 48356.0 24 54065.0 25 58120.0 26 63944.0 27 73448.0 28 88430.0 29 105049.0 30 127204.0 31 157969.0 32 199871.0 33 264340.0 34 379729.0 35 605199.0 36 1098024.0 37 1949350.0 38 1682380.0 39 352215.0 40 445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.998381656024264 17.894543842154402 13.487709004914903 23.619365496906436 2 15.21534157910072 21.908456225779467 36.999736145447635 25.87646604967218 3 18.890808467467604 27.2114020466315 29.501668841095913 24.39612064480498 4 13.351871902787064 18.166660038602846 34.58146028001444 33.90000777859565 5 13.7845002709236 36.41363135595762 33.08270817629428 16.719160196824497 6 30.881234733902225 34.28272622830216 18.107196622037275 16.728842415758336 7 25.530383403240116 32.452608144438216 22.027803587922772 19.9892048643989 8 27.57847511257241 30.737313415707224 21.235276521844998 20.448934949875373 9 27.867059209223665 14.454690523173403 19.694651761635015 37.98359850596791 10-11 24.979962581838183 25.59489908507026 25.990004511852288 23.435133821239262 12-13 27.198830256384294 23.759933359425027 26.43522056523249 22.606015818958188 14-15 25.559123369568574 23.13254195113573 25.44691720968372 25.861417469611975 16-17 22.898537345440097 26.302800494766345 27.162634786714662 23.636027373078896 18-19 23.960176570170297 25.102317678447267 29.288594154692614 21.648911596689825 20-21 24.425857725127052 24.416942438504954 28.54404011217664 22.613159724191352 22-23 23.29817734468702 25.285825818409812 26.927746110690542 24.48825072621262 24-25 23.021798535324034 26.14493214490057 26.553774415140207 24.27949490463519 26-27 23.7828384194954 24.83918123728896 26.972451624585542 24.405528718630094 28-29 23.091218377090538 24.319061825865184 29.071753996993483 23.517965800050796 30-31 24.383443576392466 24.486026618901125 28.148319896777686 22.982209907928723 32-33 22.096026311465543 25.398643624074264 28.227754191261038 24.277575873199154 34-35 22.098536413823723 25.912290864245552 28.589426868687536 23.39974585324319 36-37 23.410456521690335 25.862766414053556 27.31222421489547 23.414552849360636 38-39 23.95095112862417 24.688745317206738 27.123875744638088 24.236427809531005 40-41 23.064091192672205 25.712431062072238 27.14076264342839 24.082715101827166 42-43 24.821301267884525 25.125667611132208 26.724094294282764 23.328936826700506 44-45 24.489706335320694 24.969341602018204 27.056376036443403 23.4845760262177 46-47 23.426995218738682 25.206291158265948 28.130866551993584 23.235847071001785 48-49 24.03252808092626 26.017638782972046 27.128932679758357 22.82090045634334 50-51 23.000663037899248 26.555482231364348 25.99084227654453 24.45301245419188 52-53 22.76400511959738 26.634303746979537 24.94706789303524 25.654623240387846 54-55 23.499899369897868 26.14408074043311 24.85185809167306 25.504161797995963 56-57 24.122662873836703 24.898544375163016 25.248193809355435 25.730598941644843 58-59 23.734965289379474 24.781491028220362 26.8905879284792 24.592955753920965 60-61 23.75663072506734 25.361889020035505 26.198243810180276 24.68323644471688 62-63 22.116305435652343 25.62602696430334 26.75081260844833 25.506854991595983 64-65 22.141628847795626 25.64524463720728 26.747993218325387 25.4651332966717 66-67 23.699049494442782 25.377634763555236 25.82816697745344 25.095148764548547 68-69 23.997850729175916 24.580402131613784 25.83544860354757 25.58629853566273 70-71 23.013255493558805 25.236587629590634 26.12205851617233 25.62809836067823 72-73 24.32453221777494 24.75891824839931 26.051508338895466 24.865041194930278 74-75 24.599539927547045 24.413910672646097 26.243958483007923 24.742590916798935 76-77 23.491145166910528 25.292563156012815 27.027773285765367 24.18851839131129 78-79 23.619798303589004 25.257033623451164 26.875521140565162 24.247646932394677 80-81 22.913260200817533 25.34749147049528 26.543371021873973 25.19587730681321 82-83 23.148374599490392 25.752640576852343 25.306855429575148 25.792129394082114 84-85 23.43597003443407 25.708999340517963 25.118385448220053 25.73664517682791 86-87 23.681547230352663 24.56266747552657 25.705046890183482 26.05073840393729 88-89 23.91923368508322 24.053450726242595 26.449448273250226 25.57786731542396 90-91 24.389487428484312 24.767875700632057 25.746178564891615 25.096458305992016 92-93 23.504405728045064 25.00954025689106 26.023975102049572 25.46207891301431 94-95 23.20987148090153 25.180428482838064 26.35410669234794 25.255593343912462 96-97 24.595359694440123 24.853487130070473 25.311978213156817 25.239174962332584 98-99 24.864019320130996 24.333728749012764 25.14462271153436 25.65762921932188 100 24.79491663655655 24.714735548112344 25.454812872647892 25.035534942683217 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2480.0 1 2685.5 2 2094.0 3 1196.5 4 978.0 5 1042.0 6 1369.0 7 2378.5 8 3612.5 9 3722.5 10 2939.5 11 2553.5 12 2834.0 13 3199.0 14 3656.0 15 4226.5 16 4881.5 17 5697.5 18 6528.5 19 7583.5 20 9058.0 21 10941.0 22 13351.0 23 16143.0 24 19613.0 25 24094.0 26 30211.0 27 36746.5 28 42947.5 29 51198.5 30 60535.0 31 69615.0 32 78329.0 33 86542.5 34 94994.0 35 101634.5 36 107154.0 37 115426.5 38 123275.0 39 131617.0 40 137645.0 41 141714.5 42 150662.0 43 170095.5 44 195116.0 45 218024.0 46 250156.0 47 291261.0 48 399795.0 49 516501.0 50 625697.5 51 583480.5 52 410783.0 53 346063.0 54 311625.0 55 278414.0 56 256088.5 57 231170.5 58 213600.5 59 195160.0 60 165540.5 61 137187.0 62 111692.0 63 86568.5 64 64083.5 65 51287.0 66 40643.5 67 29480.0 68 22364.0 69 17948.0 70 15300.0 71 13526.0 72 11582.5 73 11433.0 74 9493.0 75 7514.5 76 5884.5 77 3893.5 78 2779.5 79 1967.0 80 1386.0 81 1077.0 82 849.0 83 645.0 84 458.0 85 299.5 86 216.5 87 153.0 88 122.0 89 100.0 90 83.5 91 69.0 92 62.5 93 56.5 94 38.5 95 27.0 96 17.5 97 18.5 98 22.5 99 13.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11627315932599688 2 0.3732252552328528 3 0.38396429555632966 4 0.3798032288880683 5 0.30562660929876306 6 0.1379007393742055 7 0.15033410624523783 8 0.12670074256352604 9 0.20912474578872647 10-11 0.20897524638747153 12-13 0.2224239633586934 14-15 0.15045245993789796 16-17 0.13558349865475455 18-19 0.1546135266061593 20-21 0.1552738156283684 22-23 0.1634838244139499 24-25 0.17644043918937438 26-27 0.16912119766960332 28-29 0.17694499966860966 30-31 0.22010049349752356 32-33 0.2180697932971445 34-35 0.1314784942619638 36-37 0.0923158802748995 38-39 0.08556971979327223 40-41 0.08473501480293238 42-43 0.0750736159968346 44-45 0.0969005285800497 46-47 0.1437561325900223 48-49 0.22444220527563455 50-51 0.17971696773354423 52-53 0.11175080243803624 54-55 0.060229571280567024 56-57 0.07524803196496531 58-59 0.11119017968333038 60-61 0.08628607109095195 62-63 0.09598484474736345 64-65 0.10483022598827825 66-67 0.10166582199504957 68-69 0.0912444678992394 70-71 0.12644534775304891 72-73 0.14984200404944045 74-75 0.195078031212485 76-77 0.19502819807873337 78-79 0.15592164636713965 80-81 0.09242177568412172 82-83 0.13198928388291803 84-85 0.1609983968680872 86-87 0.17271541244143984 88-89 0.16074300205761008 90-91 0.18248270665675984 92-93 0.10136059405082083 94-95 0.3451505135055267 96-97 0.34673894464386007 98-99 0.38599499575670865 100 0.3879135714061465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8026788.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.8546313365178 #Duplication Level Percentage of deduplicated Percentage of total 1 76.34821837930822 28.13785441569357 2 13.091078988710105 9.649337798522906 3 4.117320435992989 4.55226980288497 4 1.8637298549633026 2.7474830686213725 5 1.04882311502941 1.9326994620813558 6 0.6860728345753704 1.5170976832965024 7 0.44634620526798435 1.1514947390523684 8 0.3119625965110263 0.9197813188157383 9 0.24900496731497948 0.8259287644219712 >10 1.5184931561402977 10.605676525415214 >50 0.1542887431546062 3.983039487065857 >100 0.1326906511159331 10.112094527738403 >500 0.019378960101445453 4.929826821875156 >1k 0.010962073089398271 7.571869795398785 >5k 6.448278286290676E-4 1.7007157270418016 >10k+ 9.842108963285768E-4 9.662830062074011 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 93982 1.170854394061485 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 85305 1.062753868670756 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 44263 0.5514409998121291 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 40358 0.5027914029870977 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 39634 0.4937716057780522 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 38123 0.474947139503373 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 34730 0.43267618379855055 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32374 0.4033244680188389 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 30597 0.38118609834967615 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 29731 0.37039722489244764 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 29684 0.36981168557086597 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 27936 0.34803460612140247 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 23924 0.29805197296851493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22424 0.2793645478116527 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 17801 0.22176990347820325 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 17770 0.22138369669162808 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 15958 0.19880928710213847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15879 0.19782508271054375 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 14848 0.1849805924860604 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 13205 0.1645116327975773 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 13154 0.163876260342244 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 13135 0.16363955295692373 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 11791 0.14689562001637516 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 11770 0.1466339960641791 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 11728 0.14611074815978695 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 10806 0.13462421083003562 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 10665 0.13286759286529057 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 10529 0.13117326631773507 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 10438 0.1300395625248854 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 9465 0.11791765273980077 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 8650 0.10776415173790563 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 8487 0.1057334515375266 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 8413 0.10481153856312139 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 8304 0.1034535856683894 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 8266 0.10298017089774888 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 8216 0.10235725672585348 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.744159681307143E-4 0.0 0.0 1.2458283437908164E-5 0.0 2 3.3637365282352045E-4 0.0 0.0 1.2458283437908164E-5 0.0 3 3.3637365282352045E-4 0.0 0.0 1.2458283437908164E-5 0.0 4 3.9866507001306125E-4 0.0 0.0 2.4916566875816328E-5 0.0 5 0.001731701397869235 0.0 0.0 3.7374850313724493E-5 0.0 6 0.003276528544169847 0.0 0.0 4.9833133751632656E-5 0.0 7 0.0036502770473070922 0.0 0.0 4.9833133751632656E-5 0.0 8 0.004185983235137143 0.0 0.0 7.474970062744899E-5 1.2458283437908164E-5 9 0.004497440321084847 0.0 0.0 8.720798406535715E-5 1.2458283437908164E-5 10-11 0.0077303648732220155 0.0 0.0 1.8064510984966837E-4 1.2458283437908164E-5 12-13 0.015224022361123777 6.229141718954082E-6 0.0 2.491656687581633E-4 1.8687425156862247E-5 14-15 0.024007112184849033 1.8687425156862247E-5 0.0 3.0522794422875E-4 4.3603992032678575E-5 16-17 0.02720889102839143 4.3603992032678575E-5 0.0 4.796439123594643E-4 7.474970062744899E-5 18-19 0.029183528953299877 5.606227547058674E-5 0.0 6.229141718954083E-4 7.474970062744899E-5 20-21 0.037169288636999004 6.229141718954082E-5 0.0 9.84204391594745E-4 8.097884234640307E-5 22-23 0.040078297819750565 6.85205589084949E-5 0.0 0.0026847600808692093 9.343712578431122E-5 24-25 0.0579123305611161 7.474970062744899E-5 0.0 0.007175971260235102 9.966626750326531E-5 26-27 0.11545714176081391 7.474970062744899E-5 1.2458283437908164E-5 0.014258505394685895 9.966626750326531E-5 28-29 0.12785936292325148 7.474970062744899E-5 1.2458283437908164E-5 0.04292501558531259 9.966626750326531E-5 30-31 0.12995235454082005 7.474970062744899E-5 1.2458283437908164E-5 0.12391631621515356 9.966626750326531E-5 32-33 0.13124178687664356 7.474970062744899E-5 1.2458283437908164E-5 0.24218280089121574 9.966626750326531E-5 34-35 0.13245024037012065 9.343712578431124E-5 1.2458283437908164E-5 0.39635405843532934 9.966626750326531E-5 36-37 0.13423800404346048 1.1835369266012757E-4 1.2458283437908164E-5 0.6261657838727023 1.058954092222194E-4 38-39 0.13601330943336237 1.2458283437908165E-4 1.2458283437908164E-5 0.9387316570463802 1.1212455094117349E-4 40-41 0.13775746911466952 1.2458283437908165E-4 1.2458283437908164E-5 1.3492009506168594 1.1212455094117349E-4 42-43 0.14196836891668246 1.2458283437908165E-4 1.2458283437908164E-5 1.6539617092166878 1.1212455094117349E-4 44-45 0.14735657650357775 1.3081197609803572E-4 2.4916566875816328E-5 1.9715794163244376 1.1212455094117349E-4 46-47 0.14997904516725746 1.370411178169898E-4 2.4916566875816328E-5 2.3401452735515127 1.1212455094117349E-4 48-49 0.15156124716387176 1.370411178169898E-4 2.4916566875816328E-5 2.6761514070136148 1.1212455094117349E-4 50-51 0.15407159127661027 1.744159681307143E-4 2.4916566875816328E-5 3.039304140086919 1.1212455094117349E-4 52-53 0.15517414936086515 1.744159681307143E-4 2.4916566875816328E-5 3.4555291606057117 1.1212455094117349E-4 54-55 0.15776547231595006 1.744159681307143E-4 2.4916566875816328E-5 3.830068764741264 1.1212455094117349E-4 56-57 0.16323465874519172 1.744159681307143E-4 2.4916566875816328E-5 4.225395014792966 1.1212455094117349E-4 58-59 0.1651781509615054 1.744159681307143E-4 2.4916566875816328E-5 4.634070315548386 1.1212455094117349E-4 60-61 0.16628070904576026 1.744159681307143E-4 2.4916566875816328E-5 5.007793902118756 1.1212455094117349E-4 62-63 0.16712787231953802 1.744159681307143E-4 2.4916566875816328E-5 5.394816706259091 1.1212455094117349E-4 64-65 0.16778816134174715 1.8687425156862247E-4 2.4916566875816328E-5 5.818778320792825 1.1212455094117349E-4 66-67 0.16872876174130924 1.8687425156862247E-4 2.4916566875816328E-5 6.251635149701226 1.1212455094117349E-4 68-69 0.16967559128259024 1.8687425156862247E-4 2.4916566875816328E-5 6.7581266628693815 1.1212455094117349E-4 70-71 0.17076569108340722 1.8687425156862247E-4 2.4916566875816328E-5 7.348910921778424 1.1212455094117349E-4 72-73 0.17389894936804112 1.8687425156862247E-4 2.4916566875816328E-5 7.844663394623105 1.2458283437908165E-4 74-75 0.1771007282115835 1.8687425156862247E-4 2.4916566875816328E-5 8.323858808778805 1.2458283437908165E-4 76-77 0.1781472240203678 1.8687425156862247E-4 2.4916566875816328E-5 8.852825065268947 1.2458283437908165E-4 78-79 0.17903799128617823 1.8687425156862247E-4 2.4916566875816328E-5 9.374191768861966 1.2458283437908165E-4 80-81 0.18043331903122395 1.8687425156862247E-4 2.4916566875816328E-5 9.956386041340572 1.2458283437908165E-4 82-83 0.18105623320311937 1.8687425156862247E-4 2.4916566875816328E-5 10.621097754170162 1.2458283437908165E-4 84-85 0.18354788989070098 1.8687425156862247E-4 2.4916566875816328E-5 11.245756085746876 1.2458283437908165E-4 86-87 0.1880764759203806 1.8687425156862247E-4 2.4916566875816328E-5 11.862304074805515 1.2458283437908165E-4 88 0.189241325421825 1.8687425156862247E-4 2.4916566875816328E-5 12.353446982778168 1.2458283437908165E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 8140 0.0 26.67496 1 ATCTACA 9400 0.0 23.545631 2 TCTACAC 11245 0.0 21.82023 3 TACACTC 11500 0.0 20.580309 5 AGTACTT 37735 0.0 20.136768 12-13 GAGTACT 36640 0.0 19.718634 12-13 CTACACT 13375 0.0 18.903372 4 GTACTAG 2130 0.0 18.57492 1 TACTTTT 43375 0.0 17.60588 14-15 GTACTTT 41860 0.0 17.345232 14-15 CACTCTT 13445 0.0 17.208988 7 TAGTACT 2210 0.0 16.830368 4 TAGGCAT 3105 0.0 16.669127 5 GACGTTA 745 0.0 16.412249 7 AGTACTC 2695 0.0 16.237007 5 ACTTTTT 45970 0.0 16.030413 16-17 CATGGGG 17640 0.0 15.907666 4 CTAGGCA 3695 0.0 15.800325 4 CATGGGT 7785 0.0 15.72438 4 ACACTCT 14905 0.0 15.58514 6 >>END_MODULE