FastQCFastQC Report
Fri 27 May 2016
ERR765395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10405718
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA503760.48411844334047877No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG395420.38000261010340664No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT363130.3489715942715342No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG285320.2741953991065297No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT234140.22501090265948012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198080.1903568787852986No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT184050.1768739072114005No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG164940.15850900437624776No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC150730.14485305098600598No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC146150.1404516247701504No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA145570.13989423891748748No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC141460.13594448744430707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119260.11461006342858801No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC114700.11022785741454844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC103800.026.8440511
TCTACAC136100.023.481453
TACACTC130800.021.6261885
ATCTACA134950.020.995912
CTACACT161650.020.5559734
GTATAAG43400.019.5211981
GTACTAG39700.019.20651
GTGATCG25700.019.0474348
TCTAACG18450.018.3673842
CATGGGG226800.018.3657764
GTATATA48000.018.2387641
TGATCGC27650.017.875139
CTATTGA50400.017.7450879
GTTATAT36000.017.6504171
ACGCCTA20100.017.5436026
CTAACGC19800.017.3528563
AGTACTC44500.017.345915
GTATAAT43100.017.2545431
CACTCTT159100.016.8926567
GGCTATT56050.016.7952487
Produced by FastQC (version 0.11.5)