##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765395_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10405718
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.464887862615534	33.0	33.0	33.0	27.0	33.0
2	31.49006402057023	33.0	33.0	33.0	27.0	33.0
3	31.426702991566753	33.0	33.0	33.0	27.0	33.0
4	35.16971611185312	37.0	37.0	37.0	33.0	37.0
5	35.17013165261638	37.0	37.0	37.0	33.0	37.0
6	35.083791046422746	37.0	37.0	37.0	33.0	37.0
7	35.02316889617804	37.0	37.0	37.0	33.0	37.0
8	34.99423124862696	37.0	37.0	37.0	33.0	37.0
9	34.80974614149643	37.0	37.0	37.0	33.0	37.0
10-11	34.90254045900532	37.0	37.0	37.0	33.0	37.0
12-13	34.89034778762984	37.0	37.0	37.0	33.0	37.0
14-15	36.24657347047075	37.0	37.0	40.0	33.0	40.0
16-17	36.413033391833224	37.0	37.0	40.0	33.0	40.0
18-19	36.50008158975671	38.5	37.0	40.0	33.0	40.0
20-21	36.507347402649195	40.0	37.0	40.0	33.0	40.0
22-23	36.43278282190619	37.0	37.0	40.0	33.0	40.0
24-25	36.38201405227395	37.0	37.0	40.0	33.0	40.0
26-27	36.357452652474336	37.0	37.0	40.0	33.0	40.0
28-29	36.26801456660655	37.0	37.0	40.0	33.0	40.0
30-31	36.182647944139944	37.0	37.0	40.0	33.0	40.0
32-33	36.01506195920358	37.0	37.0	40.0	33.0	40.0
34-35	35.923637369377104	37.0	37.0	40.0	33.0	40.0
36-37	35.811469761144785	37.0	37.0	40.0	33.0	40.0
38-39	35.673114916241246	37.0	37.0	40.0	27.0	40.0
40-41	35.54775821332079	37.0	37.0	40.0	27.0	40.0
42-43	35.36775290277903	37.0	37.0	40.0	27.0	40.0
44-45	35.107270204708605	37.0	37.0	40.0	27.0	40.0
46-47	34.85084541979708	37.0	37.0	40.0	27.0	40.0
48-49	34.567750490643704	37.0	33.0	40.0	27.0	40.0
50-51	34.369616877951145	37.0	33.0	40.0	27.0	40.0
52-53	34.158062230785035	37.0	33.0	40.0	27.0	40.0
54-55	33.963659499517476	37.0	33.0	40.0	24.5	40.0
56-57	33.79123972992541	37.0	33.0	40.0	22.0	40.0
58-59	33.57307006590031	37.0	33.0	40.0	22.0	40.0
60-61	33.350254927146786	37.0	33.0	37.0	22.0	40.0
62-63	33.08935284427274	37.0	33.0	37.0	22.0	40.0
64-65	32.838573993644644	37.0	33.0	37.0	22.0	40.0
66-67	32.622824008876655	37.0	33.0	37.0	22.0	40.0
68-69	32.366239552138545	37.0	33.0	37.0	22.0	40.0
70-71	32.113499856521194	37.0	33.0	37.0	22.0	38.5
72-73	31.829735439688065	37.0	33.0	37.0	22.0	37.0
74-75	31.550569696391925	37.0	33.0	37.0	15.0	37.0
76-77	31.30363233945029	33.0	33.0	37.0	15.0	37.0
78-79	31.105695733826344	33.0	33.0	37.0	15.0	37.0
80-81	30.85553471658563	33.0	33.0	37.0	15.0	37.0
82-83	30.64886421100399	33.0	33.0	37.0	15.0	37.0
84-85	30.418018487527725	33.0	33.0	37.0	10.5	37.0
86-87	30.198500814648256	33.0	33.0	37.0	6.0	37.0
88-89	29.9890073899754	33.0	30.0	37.0	4.0	37.0
90-91	29.775171112651716	33.0	27.0	37.0	2.0	37.0
92-93	29.557748730073214	33.0	27.0	37.0	2.0	37.0
94-95	29.258533337151746	33.0	27.0	37.0	2.0	37.0
96-97	29.004198220632155	33.0	27.0	37.0	2.0	37.0
98-99	28.6701562064242	33.0	27.0	37.0	2.0	37.0
100	28.36070841051045	33.0	27.0	37.0	2.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	123232.0
3	27610.0
4	14872.0
5	11621.0
6	14962.0
7	25254.0
8	31350.0
9	28665.0
10	28148.0
11	30893.0
12	35515.0
13	39291.0
14	41110.0
15	48245.0
16	56629.0
17	65330.0
18	59654.0
19	41001.0
20	33029.0
21	34005.0
22	40705.0
23	51460.0
24	64544.0
25	79456.0
26	95592.0
27	115449.0
28	144167.0
29	184976.0
30	240472.0
31	316199.0
32	424461.0
33	582392.0
34	811698.0
35	1194862.0
36	1908783.0
37	2514288.0
38	845233.0
39	565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35353830087401	19.390838921823796	13.029306381330969	24.22631639597123
2	15.054982351996662	21.870697968676836	38.362903714301375	24.71141596502513
3	17.471925949656505	27.822077112442727	30.182964467418948	24.523032470481816
4	12.287496387325614	18.26676814523999	37.77839225557986	31.667343211854533
5	13.025916089249485	38.41309456658006	33.73970615320177	14.821283190968682
6	29.43377408999802	38.766914289718294	17.900640830015732	13.89867079026795
7	25.772998491070265	33.595365579773976	22.365502784825505	18.266133144330247
8	26.762361909068083	33.177628394336914	20.581907199510326	19.478102497084677
9	27.186840136844527	15.413134702400063	19.818486965950232	37.581538194805184
10-11	24.12503289621022	26.849036143443435	27.441826332231635	21.584104628114712
12-13	25.79786846697269	24.753539661177506	27.12635745512874	22.322234416721066
14-15	23.697687654941074	24.925144851943752	25.63615102392539	25.74101646918978
16-17	22.50513556799077	27.7750075095173	26.356702239986536	23.363154682505392
18-19	23.292122134480397	26.805891269918803	28.325554327728476	21.576432267872327
20-21	24.653477854976252	26.03186810541176	27.17357556513645	22.141078474475545
22-23	23.560771858300004	26.419032503755957	26.61015219942151	23.410043438522532
24-25	23.099977339382058	26.866978328645846	26.63401026243456	23.399034069537535
26-27	23.5163830117249	26.409537525425925	26.50189059515163	23.572188867697548
28-29	23.19403753597308	25.969713900980018	27.770717543407468	23.065531019639433
30-31	24.048129115165334	25.92677410631347	27.4038610310216	22.621235747499597
32-33	22.688525674057285	26.500650892134498	27.3199984854481	23.49082494836012
34-35	22.83163449172849	26.58295660136091	27.54556677396024	23.039842132950366
36-37	23.368240746889427	26.722858213422484	26.814010029294472	23.094891010393614
38-39	23.755386221306402	25.8149509721482	26.96742310333607	23.46223970320933
40-41	23.17753085370947	26.44844401895189	27.077838357718324	23.29618676962032
42-43	24.073855355296	26.175675719830195	26.78978519310248	22.96068373177132
44-45	23.699430075607275	26.2971943636352	26.864749857493674	23.13862570326385
46-47	23.188942453605737	26.287087682650164	27.199769078642234	23.324200785101862
48-49	23.84630754022523	26.534801638321138	26.85862743224934	22.760263389204294
50-51	23.32279509852434	26.859846922825504	26.210465882749105	23.606892095901046
52-53	23.010620005242373	27.07260202982097	25.723573297929313	24.193204667007347
54-55	23.395327597574855	26.641666367533805	25.789380384601728	24.173625650289612
56-57	23.651859996329698	25.94376059181896	25.779582142155395	24.624797269695947
58-59	23.31706684127375	25.824017684974383	26.862029567066543	23.99688590668533
60-61	23.604607741003733	26.225288428311917	26.215259565658467	23.954844265025887
62-63	22.48642938508678	26.336545675811962	26.44836275671438	24.728662182386874
64-65	22.46030050884062	26.06936656442123	26.407744737347276	25.062588189390866
66-67	23.30771837729863	26.264888113577967	25.820299494275883	24.607094014847522
68-69	23.510510892029952	25.530738879008602	26.104074677661686	24.85467555129976
70-71	22.820457820981094	25.737123646761102	26.26090473830502	25.181513793952785
72-73	23.521281294494997	25.586368154881807	26.30762919117896	24.58472135944424
74-75	23.589232381657855	25.420129586444684	26.47399727726621	24.516640754631254
76-77	23.005904061593828	25.79967571675496	26.60138877490241	24.593031446748796
78-79	23.140264470434474	25.723656265316126	26.590046801272383	24.54603246297702
80-81	22.914351831830793	25.643454411087756	26.67043382911882	24.771759927962627
82-83	23.011040660529144	25.81793010342967	26.25590084221002	24.91512839383116
84-85	23.125797220002017	25.735527799162977	25.877786492026335	25.260888488808668
86-87	22.774166804371582	25.42549617856244	26.26887972842204	25.53145728864394
88-89	22.973504841364008	24.899123734520913	26.722846487838602	25.404524936276474
90-91	23.284856371022226	25.113313658087016	26.44759615708035	25.154233813810407
92-93	22.789119552428048	25.360201924789656	26.451449747548367	25.399228775233933
94-95	22.13208661673656	25.525728521621293	26.76991444912367	25.57227041251847
96-97	22.7926595238514	25.41526515128582	26.248702514131438	25.543372810731345
98-99	22.609232147173113	25.29172699014627	26.400195854101188	25.69884500857943
100	22.518208019687638	25.445108684005145	26.306875345543222	25.729807950763988
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2182.0
1	1791.0
2	1516.0
3	1589.5
4	1308.0
5	1216.0
6	1562.5
7	2793.0
8	4606.0
9	4502.0
10	2828.0
11	1984.0
12	1992.5
13	2189.5
14	2534.0
15	3194.5
16	3881.5
17	4481.0
18	5455.5
19	6780.5
20	8456.0
21	10568.5
22	13206.5
23	16855.0
24	21673.5
25	27647.5
26	36557.5
27	46995.0
28	57122.0
29	71783.5
30	87293.0
31	100397.5
32	117711.5
33	137177.5
34	158486.0
35	175767.0
36	194835.5
37	221301.0
38	232986.5
39	235939.0
40	245099.5
41	253186.5
42	259846.5
43	279633.5
44	306234.0
45	332887.0
46	371693.5
47	413765.5
48	468328.5
49	517878.0
50	569322.5
51	568516.0
52	502452.5
53	460218.0
54	416841.5
55	363455.0
56	325212.5
57	290757.0
58	266207.0
59	244351.5
60	206321.0
61	162182.5
62	125380.0
63	96466.5
64	70858.5
65	54417.5
66	42031.0
67	30335.5
68	22706.0
69	18194.5
70	15852.5
71	14278.0
72	12539.5
73	12991.0
74	10372.0
75	7397.5
76	5525.5
77	3849.5
78	2827.5
79	1670.5
80	1084.5
81	785.5
82	535.5
83	345.0
84	230.0
85	165.0
86	130.5
87	91.5
88	56.5
89	36.0
90	26.5
91	21.5
92	15.5
93	8.5
94	8.0
95	6.5
96	5.5
97	6.0
98	4.0
99	7.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008178195872692303
2	0.04908839543796978
3	0.04814660554898759
4	0.04647444799099879
5	0.07806284967553416
6	0.01794205839520156
7	0.015904717002709474
8	0.01985446847588989
9	0.0060063130674884715
10-11	0.017144420019839093
12-13	0.03350561681567769
14-15	0.009187256468030366
16-17	0.005655544384347145
18-19	0.002436160580173324
20-21	0.008298322134042073
22-23	0.011950160479075062
24-25	0.0
26-27	0.0
28-29	1.922020181596311E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	9.610100907981555E-6
38-39	0.0
40-41	0.0
42-43	0.0
44-45	3.795989858652714E-4
46-47	0.0042668848031438104
48-49	0.0041515635922480315
50-51	0.0011243818062338419
52-53	0.0011580171594117773
54-55	0.006174489833378148
56-57	0.0055402231734513665
58-59	0.013506996826168075
60-61	0.007202770630532174
62-63	0.0031809434005418942
64-65	0.0019268252320503016
66-67	0.001379049480295353
68-69	3.459636326873359E-4
70-71	3.363535317793544E-4
72-73	5.189454490310039E-4
74-75	0.0
76-77	0.0
78-79	1.7298181634366796E-4
80-81	7.688080726385244E-5
82-83	0.0
84-85	2.5947272451550197E-4
86-87	1.2973636225775098E-4
88-89	2.3544747224554807E-4
90-91	8.456888799023768E-4
92-93	5.910212058408656E-4
94-95	3.699888849572898E-4
96-97	1.633717154356864E-4
98-99	1.2493131180376022E-4
100	9.610100907981555E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0405718E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.0908705036605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79128129633736	28.488650106616316
2	13.276993822780772	10.114645047628855
3	4.777946360488385	5.459884082723971
4	2.199808139877424	3.351704279558769
5	1.2093329014005358	2.303227147153192
6	0.7468705724517435	1.7069370154952486
7	0.493242012925881	1.3151612348927197
8	0.3532327549358562	1.0763954500730353
9	0.2750445762854847	0.942901860422211
>10	1.548336633304197	11.156709432438422
>50	0.15720017813865	4.164794695430778
>100	0.13493993186432213	10.717226608622589
>500	0.02135724436393788	5.639305577080497
>1k	0.013571720117726465	9.36371406493706
>5k	4.8470423741038446E-4	1.1410014317980621
>10k+	3.571504907234412E-4	3.0577419651279847
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	50376	0.48411844334047877	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	39542	0.38000261010340664	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	36313	0.3489715942715342	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	28532	0.2741953991065297	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	23414	0.22501090265948012	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19808	0.1903568787852986	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	18405	0.1768739072114005	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	16494	0.15850900437624776	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	15073	0.14485305098600598	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	14615	0.1404516247701504	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	14557	0.13989423891748748	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	14146	0.13594448744430707	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11926	0.11461006342858801	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	11470	0.11022785741454844	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.610100907981554E-4	0.0	0.0	4.805050453990777E-5	0.0
2	0.0012589232189455836	0.0	0.0	7.688080726385243E-5	0.0
3	0.0013069737234854913	0.0	0.0	7.688080726385243E-5	0.0
4	0.0015856666498169564	0.0	0.0	8.649090817183398E-5	0.0
5	0.005756450443880951	0.0	0.0	3.555737335953175E-4	0.0
6	0.01310817763848684	0.0	0.0	9.513999898901739E-4	9.610100907981553E-6
7	0.015116688728254984	0.0	0.0	0.0011147717053258603	9.610100907981553E-6
8	0.01831685233061284	0.0	0.0	0.0012877535216695283	2.8830302723944663E-5
9	0.020575226043988506	0.0	0.0	0.0014126848334732884	3.844040363192621E-5
10-11	0.04525396517568514	0.0	0.0	0.0028109545155846046	3.844040363192621E-5
12-13	0.07506930324269791	0.0	0.0	0.004795440353082795	3.844040363192621E-5
14-15	0.10759949481621547	0.0	0.0	0.006799146392396949	5.766060544788933E-5
16-17	0.12073650275742626	0.0	0.0	0.007678470625477262	5.766060544788933E-5
18-19	0.12685333198535653	0.0	0.0	0.007957163551808727	6.727070635587087E-5
20-21	0.1511380569798259	0.0	0.0	0.009547635252079674	6.727070635587087E-5
22-23	0.15849458922488577	0.0	0.0	0.011671467552743597	8.16858577178432E-5
24-25	0.19393664137352173	0.0	0.0	0.018931898788723662	9.610100907981553E-5
26-27	0.2903019282283068	0.0	0.0	0.03810885515060085	1.057111099877971E-4
28-29	0.3119775108262592	0.0	0.0	0.06259058721368387	1.1051616044178788E-4
30-31	0.3202421976071233	0.0	0.0	0.1089977644983268	1.249313118037602E-4
32-33	0.3273873076322076	0.0	0.0	0.16654785378577433	1.249313118037602E-4
34-35	0.3332206388833524	0.0	0.0	0.2345969783151917	1.249313118037602E-4
36-37	0.3516047619203211	0.0	4.805050453990777E-6	0.3420186862646095	1.249313118037602E-4
38-39	0.3704645849522349	0.0	9.610100907981553E-6	0.5125547319271961	1.2973636225775096E-4
40-41	0.3833997807743781	9.610100907981553E-6	9.610100907981553E-6	0.7870672643636892	1.3454141271174175E-4
42-43	0.4109663552289232	9.610100907981553E-6	9.610100907981553E-6	1.0041306135722685	1.3934646316573254E-4
44-45	0.44005613067738336	9.610100907981553E-6	9.610100907981553E-6	1.2209969557122342	1.5376161452770485E-4
46-47	0.4488782033109104	9.610100907981553E-6	9.610100907981553E-6	1.4758183913882732	1.5376161452770485E-4
48-49	0.45503347294247254	9.610100907981553E-6	9.610100907981553E-6	1.7331000128967555	1.633717154356864E-4
50-51	0.46624846070208703	9.610100907981553E-6	9.610100907981553E-6	2.024569568385382	1.633717154356864E-4
52-53	0.47032314348707127	9.610100907981553E-6	9.610100907981553E-6	2.3551282093172237	1.7298181634366796E-4
54-55	0.4833111948642083	1.9220201815963107E-5	9.610100907981553E-6	2.6518400748511537	1.8739696770564028E-4
56-57	0.5099215642784092	1.9220201815963107E-5	9.610100907981553E-6	2.9786315562270667	1.9220201815963107E-4
58-59	0.5177489914679603	1.9220201815963107E-5	9.610100907981553E-6	3.326570064650993	2.0181211906761265E-4
60-61	0.5238177701913506	1.9220201815963107E-5	9.610100907981553E-6	3.6570374096242086	2.0181211906761265E-4
62-63	0.5294781196261518	2.4025252269953883E-5	9.610100907981553E-6	3.9970764151017737	2.0181211906761265E-4
64-65	0.5337450044292955	2.8830302723944663E-5	9.610100907981553E-6	4.360650557702986	2.0181211906761265E-4
66-67	0.540755573041668	3.844040363192621E-5	9.610100907981553E-6	4.745107449577242	2.114222199755942E-4
68-69	0.5471414850950218	3.844040363192621E-5	9.610100907981553E-6	5.172449416753366	2.114222199755942E-4
70-71	0.5546133385509775	3.844040363192621E-5	9.610100907981553E-6	5.640312374407994	2.2103232088357575E-4
72-73	0.5768222817493228	3.844040363192621E-5	9.610100907981553E-6	6.089661472663395	2.498626236075204E-4
74-75	0.5981326805127719	3.844040363192621E-5	9.610100907981553E-6	6.531654038673737	2.594727245155019E-4
76-77	0.6042687299425181	3.844040363192621E-5	9.610100907981553E-6	6.991694374189268	2.690828254234835E-4
78-79	0.6082521167688766	3.844040363192621E-5	9.610100907981553E-6	7.460883525769198	2.690828254234835E-4
80-81	0.6177036510118763	3.844040363192621E-5	9.610100907981553E-6	8.001052882655479	2.690828254234835E-4
82-83	0.6211921176414736	3.844040363192621E-5	9.610100907981553E-6	8.606037565115642	2.786929263314651E-4
84-85	0.6350402730498751	3.844040363192621E-5	9.610100907981553E-6	9.182408172122289	2.786929263314651E-4
86-87	0.6607376828778178	3.844040363192621E-5	9.610100907981553E-6	9.759773424572913	2.786929263314651E-4
88	0.6662106353449132	3.844040363192621E-5	9.610100907981553E-6	10.206494160229981	2.883030272394466E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	10380	0.0	26.844051	1
TCTACAC	13610	0.0	23.48145	3
TACACTC	13080	0.0	21.626188	5
ATCTACA	13495	0.0	20.99591	2
CTACACT	16165	0.0	20.555973	4
GTATAAG	4340	0.0	19.521198	1
GTACTAG	3970	0.0	19.2065	1
GTGATCG	2570	0.0	19.047434	8
TCTAACG	1845	0.0	18.367384	2
CATGGGG	22680	0.0	18.365776	4
GTATATA	4800	0.0	18.238764	1
TGATCGC	2765	0.0	17.87513	9
CTATTGA	5040	0.0	17.745087	9
GTTATAT	3600	0.0	17.650417	1
ACGCCTA	2010	0.0	17.543602	6
CTAACGC	1980	0.0	17.352856	3
AGTACTC	4450	0.0	17.34591	5
GTATAAT	4310	0.0	17.254543	1
CACTCTT	15910	0.0	16.892656	7
GGCTATT	5605	0.0	16.795248	7
>>END_MODULE