##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765393_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6904944
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.393871405763754	34.0	31.0	34.0	31.0	34.0
2	32.43216252007257	34.0	31.0	34.0	31.0	34.0
3	32.46557234931956	34.0	31.0	34.0	31.0	34.0
4	35.91336410548732	37.0	35.0	37.0	35.0	37.0
5	35.922749409698326	37.0	37.0	37.0	35.0	37.0
6	36.02605683695624	37.0	37.0	37.0	35.0	37.0
7	35.97999158284267	37.0	37.0	37.0	35.0	37.0
8	36.01373117580678	37.0	37.0	37.0	35.0	37.0
9	37.73833415593233	39.0	38.0	39.0	35.0	39.0
10-11	37.67240892902245	39.0	38.0	39.0	35.0	39.0
12-13	37.65390667904041	39.0	38.0	39.0	35.0	39.0
14-15	39.057016175656166	41.0	39.0	41.0	36.0	41.0
16-17	39.003120155645	41.0	39.0	41.0	36.0	41.0
18-19	38.993033976814296	41.0	39.0	41.0	36.0	41.0
20-21	38.9679285595944	41.0	39.0	41.0	36.0	41.0
22-23	38.88842972513608	41.0	39.0	41.0	35.0	41.0
24-25	38.81841771634933	41.0	39.0	41.0	35.0	41.0
26-27	38.68781376069089	40.5	38.5	41.0	35.0	41.0
28-29	38.53463235907489	40.0	38.0	41.0	35.0	41.0
30-31	38.30229673115379	40.0	38.0	41.0	34.0	41.0
32-33	38.01749833163021	40.0	38.0	41.0	34.0	41.0
34-35	37.881469784548585	40.0	38.0	41.0	33.5	41.0
36-37	37.73917340966125	40.0	37.5	41.0	33.0	41.0
38-39	37.533051245600255	40.0	37.0	41.0	33.0	41.0
40-41	37.27737610906041	40.0	37.0	41.0	33.0	41.0
42-43	37.00161297470335	40.0	36.5	41.0	32.0	41.0
44-45	36.614280434424956	40.0	36.0	41.0	31.0	41.0
46-47	36.18026974585167	39.0	35.0	41.0	30.0	41.0
48-49	35.805633398330244	39.0	35.0	41.0	28.5	41.0
50-51	34.54309672895248	38.0	34.0	40.0	26.0	40.5
52-53	34.484573082707115	38.0	34.5	39.5	26.0	40.0
54-55	35.29166000477339	39.0	35.0	40.5	27.5	41.0
56-57	35.279702268403625	39.0	35.0	41.0	28.0	41.0
58-59	35.06291303738307	38.5	35.0	41.0	27.0	41.0
60-61	34.69599919999351	38.0	35.0	40.0	26.0	41.0
62-63	34.28665482297902	37.0	35.0	40.0	26.0	41.0
64-65	33.99197256342701	36.5	34.5	39.5	25.5	41.0
66-67	33.64716165692292	36.0	34.0	39.0	25.0	41.0
68-69	33.383670888569114	36.0	34.0	39.0	23.5	41.0
70-71	33.00188386755924	35.0	34.0	38.5	23.5	40.5
72-73	32.59519845490419	35.0	34.0	37.0	22.5	39.0
74-75	32.203437421071044	35.0	34.0	37.0	20.0	39.0
76-77	31.82139869635438	35.0	33.5	36.0	20.0	38.5
78-79	31.510721810343426	35.0	33.0	36.0	18.0	37.0
80-81	31.221443070356543	35.0	33.0	35.0	15.5	37.0
82-83	30.993379453910126	35.0	33.0	35.0	9.5	36.5
84-85	30.753154623701512	35.0	33.0	35.0	6.0	36.0
86-87	30.510471916933724	35.0	33.0	35.0	2.0	36.0
88-89	30.28903992559534	35.0	32.5	35.0	2.0	35.5
90-91	30.063752291111992	35.0	32.0	35.0	2.0	35.0
92-93	29.785878422764906	35.0	32.0	35.0	2.0	35.0
94-95	29.5016682249704	35.0	31.5	35.0	2.0	35.0
96-97	29.223914632761684	35.0	31.0	35.0	2.0	35.0
98-99	28.913652087547703	35.0	31.0	35.0	2.0	35.0
100	28.347358501386832	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4290116241161499
1101	2	0.4987339682771861
1101	3	0.49579029390476137
1101	4	0.4492415684898745
1101	5	0.46379918009380106
1101	6	0.43823954122903075
1101	7	0.4789715346969672
1101	8	0.38167084137436547
1101	9	0.4771809551152373
1101	10-11	0.4511756005209264
1101	12-13	0.5360953038930134
1101	14-15	0.6466835896033132
1101	16-17	0.6737493535907646
1101	18-19	0.6037712427426243
1101	20-21	0.6175673931610461
1101	22-23	0.6782404960909858
1101	24-25	0.6463652513085307
1101	26-27	0.7085650534087478
1101	28-29	0.7065485767219855
1101	30-31	0.6914163661665071
1101	32-33	0.6498177610841154
1101	34-35	0.7886690738231295
1101	36-37	0.7588819433206098
1101	38-39	0.7824583096496625
1101	40-41	0.7619652535673893
1101	42-43	0.7045560134631401
1101	44-45	0.7033117913710782
1101	46-47	0.7376606274659494
1101	48-49	0.7393407275982113
1101	50-51	0.6968958190890291
1101	52-53	0.7261868848266531
1101	54-55	0.8074174648189185
1101	56-57	0.8278480526082888
1101	58-59	0.8750431115869048
1101	60-61	0.8682496923698437
1101	62-63	0.872400192177679
1101	64-65	0.815131666565172
1101	66-67	0.7912495893157327
1101	68-69	0.7760284763645586
1101	70-71	0.750944542412519
1101	72-73	0.6871972129524764
1101	74-75	0.6591411577190271
1101	76-77	0.6145662792325446
1101	78-79	0.6515947328948464
1101	80-81	0.6956789629399509
1101	82-83	0.6720994185145557
1101	84-85	0.6759122287719848
1101	86-87	0.6766706377561214
1101	88-89	0.7473475975329826
1101	90-91	0.8142254351246763
1101	92-93	0.7855940725575756
1101	94-95	0.7360794767138259
1101	96-97	0.6203122716011578
1101	98-99	0.7484532054072197
1101	100	0.8716644752620013
1102	1	0.43185404728189525
1102	2	0.5506052372275505
1102	3	0.5519171800799754
1102	4	0.4606973440397226
1102	5	0.4273975122800664
1102	6	0.42988654738015697
1102	7	0.46376566789503926
1102	8	0.4167158597703633
1102	9	0.43889010551512087
1102	10-11	0.4910781833873088
1102	12-13	0.4927219771818798
1102	14-15	0.5984845575294173
1102	16-17	0.6186455456467428
1102	18-19	0.6358356411814867
1102	20-21	0.6102585762901498
1102	22-23	0.6088969329599436
1102	24-25	0.6632898220228753
1102	26-27	0.5943934049763158
1102	28-29	0.6082134366326315
1102	30-31	0.5712265112035055
1102	32-33	0.6481153032067013
1102	34-35	0.6738180457444969
1102	36-37	0.6636651980048498
1102	38-39	0.6711292953704557
1102	40-41	0.6886000583132486
1102	42-43	0.6857329775138297
1102	44-45	0.6551477832168331
1102	46-47	0.6354059731879076
1102	48-49	0.674556181670674
1102	50-51	0.6107348668772659
1102	52-53	0.693733003522297
1102	54-55	0.6859682256608295
1102	56-57	0.7231900400849156
1102	58-59	0.7339551589291133
1102	60-61	0.6880185007421673
1102	62-63	0.6570208092822654
1102	64-65	0.7612802802298191
1102	66-67	0.6772129728626695
1102	68-69	0.6794495959568962
1102	70-71	0.6617877791459463
1102	72-73	0.6904664637655245
1102	74-75	0.6261087487780586
1102	76-77	0.6085343585165752
1102	78-79	0.5769155076914636
1102	80-81	0.5531790821478708
1102	82-83	0.5469112704401731
1102	84-85	0.5566673866771126
1102	86-87	0.557516309284928
1102	88-89	0.5268739222283472
1102	90-91	0.5542165061350381
1102	92-93	0.5962094006283216
1102	94-95	0.5960188220365907
1102	96-97	0.6368587527732714
1102	98-99	0.6027302800575427
1102	100	0.6595338773952548
1103	1	0.4171474667120876
1103	2	0.46629521478526925
1103	3	0.4939565340613399
1103	4	0.3887199956959364
1103	5	0.4031346059301768
1103	6	0.38095041574769084
1103	7	0.3598739829496793
1103	8	0.3821307586877367
1103	9	0.4224258228005269
1103	10-11	0.44434405241720043
1103	12-13	0.4636795024227567
1103	14-15	0.6170189228907077
1103	16-17	0.6214564913606822
1103	18-19	0.5913980976818252
1103	20-21	0.6350043943222445
1103	22-23	0.6086304094523598
1103	24-25	0.5805958840827614
1103	26-27	0.6196185668342054
1103	28-29	0.5482462034552427
1103	30-31	0.6295446996814036
1103	32-33	0.6853086772247252
1103	34-35	0.604852647804492
1103	36-37	0.5621379361375674
1103	38-39	0.5620556773950867
1103	40-41	0.6120009149509471
1103	42-43	0.6149445593997385
1103	44-45	0.5960182703688659
1103	46-47	0.5749036233297105
1103	48-49	0.6014863587824664
1103	50-51	0.5878111114127691
1103	52-53	0.5791234470235267
1103	54-55	0.682937107682136
1103	56-57	0.703343120586041
1103	58-59	0.7484517765549015
1103	60-61	0.6615432299565285
1103	62-63	0.6931058310225566
1103	64-65	0.7742569601333855
1103	66-67	0.7950866391755511
1103	68-69	0.7912057477058951
1103	70-71	0.7654046464759645
1103	72-73	0.8012653642726519
1103	74-75	0.8431112125141951
1103	76-77	0.7930746729618043
1103	78-79	0.8329526219239405
1103	80-81	0.8199409101865243
1103	82-83	0.7860481902198408
1103	84-85	0.7876623345615457
1103	86-87	0.8201951967401833
1103	88-89	0.7995482443719268
1103	90-91	0.7735869144698704
1103	92-93	0.7636872063218725
1103	94-95	0.7336834650954174
1103	96-97	0.8065596573623353
1103	98-99	0.7550125235095884
1103	100	0.7142859238852566
1104	1	0.3973899611076277
1104	2	0.4952244613304302
1104	3	0.4848240811726754
1104	4	0.42108336990845885
1104	5	0.42160371150219333
1104	6	0.40813797852348443
1104	7	0.3694191334598358
1104	8	0.3720001238955106
1104	9	0.38999567043788375
1104	10-11	0.41966767981323727
1104	12-13	0.42590770381342935
1104	14-15	0.5463240972483163
1104	16-17	0.5362637831769916
1104	18-19	0.5326551763099658
1104	20-21	0.5088546995748331
1104	22-23	0.5411209015706859
1104	24-25	0.5468318417771982
1104	26-27	0.5087240592346518
1104	28-29	0.556660639789385
1104	30-31	0.5330512102481606
1104	32-33	0.5870964220524613
1104	34-35	0.5847781262735339
1104	36-37	0.5768280474444794
1104	38-39	0.5511670312728967
1104	40-41	0.5591921480730875
1104	42-43	0.5690261437801496
1104	44-45	0.5982609633392499
1104	46-47	0.4805961014965803
1104	48-49	0.476396777985407
1104	50-51	0.45628150639441856
1104	52-53	0.5196863357718797
1104	54-55	0.5218925843385094
1104	56-57	0.46945104884571265
1104	58-59	0.45034377318062724
1104	60-61	0.46630187554911373
1104	62-63	0.4598174020288752
1104	64-65	0.5003013588629202
1104	66-67	0.49158156224769556
1104	68-69	0.40273089777068094
1104	70-71	0.3645993048701044
1104	72-73	0.39901317236280676
1104	74-75	0.3192137212149646
1104	76-77	0.33456866835130583
1104	78-79	0.35359810384987966
1104	80-81	0.4392446041749416
1104	82-83	0.40000842067282605
1104	84-85	0.3621779088415167
1104	86-87	0.347048147619045
1104	88-89	0.3983063640469098
1104	90-91	0.38257091794429954
1104	92-93	0.4722452488515039
1104	94-95	0.4232404700974044
1104	96-97	0.4294686647338324
1104	98-99	0.34344818346867356
1104	100	0.33999055596390093
1105	1	0.3067560562753684
1105	2	0.3342115470275999
1105	3	0.36005507554139626
1105	4	0.30940032431603015
1105	5	0.3266206246239918
1105	6	0.3279718879255782
1105	7	0.3356662064306306
1105	8	0.3595536793195393
1105	9	0.3747539201768717
1105	10-11	0.41739168238031255
1105	12-13	0.4314409825672314
1105	14-15	0.5070582644585215
1105	16-17	0.49988482317806415
1105	18-19	0.5018043723379364
1105	20-21	0.43757571476671586
1105	22-23	0.5048331287311143
1105	24-25	0.5279757784023289
1105	26-27	0.44072472018433473
1105	28-29	0.4740171015054031
1105	30-31	0.5132426472079175
1105	32-33	0.48902843164870546
1105	34-35	0.5179803352667562
1105	36-37	0.5708211588310661
1105	38-39	0.5124471553964369
1105	40-41	0.526441677122854
1105	42-43	0.48456678877941783
1105	44-45	0.6057942411497379
1105	46-47	0.5212251561152712
1105	48-49	0.6946878845017537
1105	50-51	0.5999127200525365
1105	52-53	0.6527378344013997
1105	54-55	0.6867796951593164
1105	56-57	0.7001573657156399
1105	58-59	0.7067817831539855
1105	60-61	0.656416241541038
1105	62-63	0.6809552380234223
1105	64-65	0.6303327413954705
1105	66-67	0.6161561400852165
1105	68-69	0.6175568529273505
1105	70-71	0.6260829388188469
1105	72-73	0.610189531787789
1105	74-75	0.6176126686007066
1105	76-77	0.5396973407700294
1105	78-79	0.603137700406446
1105	80-81	0.6005952385074096
1105	82-83	0.5667823326973398
1105	84-85	0.5790064178675287
1105	86-87	0.576850951718523
1105	88-89	0.5941325050367219
1105	90-91	0.6199438939693103
1105	92-93	0.6697348480126699
1105	94-95	0.5934941286677322
1105	96-97	0.5602539528281341
1105	98-99	0.5751379459911057
1105	100	0.6663324195590192
1106	1	0.22229697332956988
1106	2	0.1899484325896168
1106	3	0.2308604588044858
1106	4	0.253803661094544
1106	5	0.24967147896833097
1106	6	0.27218176726754706
1106	7	0.24387213314615508
1106	8	0.2286875585573256
1106	9	0.28927293328542447
1106	10-11	0.22726850412857402
1106	12-13	0.26683408677846643
1106	14-15	0.3402254166422978
1106	16-17	0.3070735084719445
1106	18-19	0.35161005557197456
1106	20-21	0.3324209707219552
1106	22-23	0.34083581523074
1106	24-25	0.36609314941662063
1106	26-27	0.3547182786318075
1106	28-29	0.34676630234108075
1106	30-31	0.32852812919615104
1106	32-33	0.2770679599351027
1106	34-35	0.28228849493410735
1106	36-37	0.2848283991507472
1106	38-39	0.35558305831888504
1106	40-41	0.3779987555395721
1106	42-43	0.38400230804618474
1106	44-45	0.37539722999853353
1106	46-47	0.3819303858592775
1106	48-49	0.3192735228398007
1106	50-51	0.27542487917857983
1106	52-53	0.31997456704129945
1106	54-55	0.36767697323219295
1106	56-57	0.39331225572604467
1106	58-59	0.41315788944432796
1106	60-61	0.36827090982520616
1106	62-63	0.3351387207878034
1106	64-65	0.34181943856696506
1106	66-67	0.34611355730398685
1106	68-69	0.3432460989035775
1106	70-71	0.30783701886434756
1106	72-73	0.23531706517875506
1106	74-75	0.302330051043846
1106	76-77	0.23948807119088755
1106	78-79	0.2060001170496868
1106	80-81	0.20633661328330533
1106	82-83	0.21946325271033373
1106	84-85	0.19759386469682028
1106	86-87	0.17927814328895053
1106	88-89	0.21410751612255297
1106	90-91	0.20116549547914886
1106	92-93	0.2307318309239541
1106	94-95	0.23699172983884864
1106	96-97	0.29062816888261267
1106	98-99	0.2724752909405197
1106	100	0.31987099605986913
1107	1	0.22060759291419174
1107	2	0.2551202007787623
1107	3	0.3148766559569154
1107	4	0.25381672331425165
1107	5	0.29130948131953005
1107	6	0.24405445416613958
1107	7	0.35950325606164313
1107	8	0.307509346339792
1107	9	0.3216672381576302
1107	10-11	0.3258468992171828
1107	12-13	0.2835384887465082
1107	14-15	0.3255848453878798
1107	16-17	0.3845477105880235
1107	18-19	0.3552239363574543
1107	20-21	0.4154662095428705
1107	22-23	0.3709093990684309
1107	24-25	0.39357823671579695
1107	26-27	0.4596100799119043
1107	28-29	0.428474840678696
1107	30-31	0.40604804908120684
1107	32-33	0.3885387658740669
1107	34-35	0.4563469265969218
1107	36-37	0.46750136471002435
1107	38-39	0.47873148867548565
1107	40-41	0.41958450901194766
1107	42-43	0.3665555365914841
1107	44-45	0.3673226011832753
1107	46-47	0.3565504929694825
1107	48-49	0.32654700217972277
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2316	100	-0.5628039254547375
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19035.0
3	2794.0
4	2759.0
5	3163.0
6	3746.0
7	4372.0
8	5462.0
9	6560.0
10	8787.0
11	13039.0
12	21504.0
13	31099.0
14	31853.0
15	37766.0
16	49783.0
17	68853.0
18	68660.0
19	40768.0
20	35206.0
21	32565.0
22	33503.0
23	38366.0
24	43074.0
25	45693.0
26	50235.0
27	57149.0
28	66960.0
29	79383.0
30	97669.0
31	121871.0
32	158401.0
33	217124.0
34	321523.0
35	527192.0
36	974521.0
37	1777695.0
38	1521562.0
39	284928.0
40	321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.414596318307	17.39236121176982	13.211784316567618	23.981258153355558
2	14.923760629143642	21.79291414629187	36.41544974620764	26.86787547835685
3	19.35969711722242	27.11257193582157	29.245811078203666	24.281919868752347
4	13.53526524115731	18.154509922513657	33.890438460835675	34.41978637549336
5	13.850876323813106	36.18838005404072	33.09144120885236	16.86930241329382
6	31.494810940495004	32.99481391441748	18.226706607170808	17.283668537916714
7	25.621257586030634	32.11195586140614	21.88347572564508	20.383310826918148
8	27.94026098650858	30.572129258639112	21.32026080085216	20.16734895400014
9	28.148031409678453	14.48056065036743	19.44477700396619	37.92663093598792
10-11	24.935334815825154	25.6363118713601	25.791801512048764	23.63655180076599
12-13	27.408591042543506	23.579565250717728	26.083425372336965	22.928418334401798
14-15	26.183884278338255	22.827491583214478	24.974221599659266	26.014402538788005
16-17	23.33913541700663	26.02961963715219	27.39135417006632	23.23989077577485
18-19	24.747459910106254	24.931286333047314	28.81652183317785	21.504731923668587
20-21	24.474443064093858	24.534516250489876	28.176798747134114	22.814241938282148
22-23	23.141650075465545	25.71972253283375	25.80828792150887	25.330339470191838
24-25	23.209537264953507	26.62844151350595	25.219510223810065	24.942510997730476
26-27	24.27457147859067	24.615492103577473	25.771618586507827	25.338317831324026
28-29	23.520373532235464	23.96944331211587	28.503219994117295	24.00696316153137
30-31	25.282661031958327	24.232258647167626	27.423234576387046	23.061845744487
32-33	21.92615407839198	25.354083327043554	27.91730290239612	24.80245969216835
34-35	21.762452385139632	25.997218802634702	28.25927141331602	23.981057398909638
36-37	23.851286840395762	25.874097577184102	26.533127113605964	23.741488468814172
38-39	24.708612710879724	24.271223680177602	26.37194516365969	24.648218445282986
40-41	23.371287880852655	25.715277540182306	26.41218693695914	24.5012476420059
42-43	25.456837456821795	24.892923566878057	26.073972383059807	23.57626659324034
44-45	25.166914128129232	24.337214286061293	26.688793059255232	23.807078526554246
46-47	23.622653014609917	24.821268789161167	28.395566496601255	23.160511699627666
48-49	24.405087263881406	25.659880382905314	27.343219793236933	22.591812559976347
50-51	22.996177632163764	26.220050106217197	26.22726307940983	24.55650918220921
52-53	22.55804938350742	26.684479562327173	24.601040783980114	26.156430270185293
54-55	23.5028354634035	26.11737550232091	24.424591167848565	25.955197866427028
56-57	24.223629502938007	24.370723840585377	24.96118917822412	26.44445747825249
58-59	23.982120061828393	24.144334026471114	26.96207833433451	24.911467577365983
60-61	24.181316739833054	24.879738349623167	26.098108451743897	24.84083645879988
62-63	21.784440243885108	25.365948030695346	26.89423262945121	25.955379095968347
64-65	21.828035392358682	25.357665749080553	26.823785769112213	25.99051308944855
66-67	23.89418543770559	25.040681680404603	25.503980881676373	25.561152000213433
68-69	24.5160712301397	23.986390809236834	25.456191820089728	26.041346140533733
70-71	23.16221207906462	24.729463096693465	25.876270862379226	26.23205396186269
72-73	24.834409541677175	24.08451943248839	25.74404655062516	25.33702447520928
74-75	25.007313144797365	23.35568872701946	26.212589056401182	25.424409071782
76-77	23.522072743135876	24.520005588238238	27.4291536299829	24.528768038642987
78-79	23.901341322109577	24.356449076993066	27.165077593818904	24.577132007078454
80-81	22.986249124922566	24.584634502862514	26.67070747428117	25.758408897933755
82-83	23.003326456785103	25.2853136639254	25.04130968694121	26.670050192348288
84-85	23.471002454160487	25.032814141500452	24.913751125509638	26.582432278829423
86-87	23.868110907429436	23.54756618453405	25.583682457010415	27.000640451026097
88-89	24.018016192261694	22.935393791223127	26.818234866638583	26.2283551498766
90-91	24.636890829765274	23.59558956441407	26.065905558394142	25.701614047426514
92-93	23.23536258736323	24.06519592977113	26.468885128401954	26.23055635446368
94-95	22.800905192107688	24.227987540563625	26.850098468965474	26.121008798363217
96-97	24.546540276176756	23.786586908819984	25.568704153494124	26.09816866150913
98-99	24.95980762334799	23.108566240206745	25.448652493861356	26.48297364258391
100	24.5711124616453	23.563787866313408	25.971300922258393	25.8937987497829
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2719.0
1	3345.5
2	2601.5
3	1111.5
4	877.5
5	898.0
6	1187.5
7	1934.5
8	2893.0
9	3078.0
10	2511.0
11	2164.5
12	2337.0
13	2607.0
14	2884.0
15	3267.5
16	3784.0
17	4361.0
18	4925.0
19	5636.0
20	6491.5
21	7555.0
22	9037.5
23	10640.0
24	12646.0
25	15442.5
26	19443.5
27	24231.0
28	28681.5
29	34762.0
30	42111.5
31	49314.0
32	57535.5
33	65418.5
34	73196.5
35	79759.5
36	85473.5
37	93165.0
38	97977.0
39	101380.0
40	105791.0
41	109428.0
42	115782.5
43	130863.0
44	149984.0
45	166791.0
46	193333.0
47	232151.0
48	336552.0
49	471787.5
50	627722.5
51	593416.0
52	388241.5
53	308196.0
54	269891.5
55	233158.0
56	211497.5
57	191211.0
58	181634.0
59	168486.5
60	144260.5
61	120328.5
62	100067.5
63	79741.5
64	59718.0
65	48263.5
66	37956.5
67	27696.0
68	21705.5
69	18402.5
70	16138.0
71	14050.0
72	11976.0
73	12095.0
74	10043.0
75	8006.5
76	6298.0
77	4143.5
78	2900.5
79	1938.5
80	1352.0
81	1023.0
82	787.0
83	588.5
84	405.5
85	260.5
86	179.0
87	138.0
88	114.0
89	86.5
90	62.5
91	54.0
92	46.5
93	39.5
94	32.5
95	28.5
96	25.5
97	16.0
98	13.5
99	12.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08608324701836828
2	0.2808712134377918
3	0.2844338781024147
4	0.29322468075048835
5	0.31840953380650155
6	0.16931346582970117
7	0.18588130475786627
8	0.15189116667709399
9	0.20198570763209664
10-11	0.2232820425480641
12-13	0.239828157911201
14-15	0.16764799251087337
16-17	0.15740895219425385
18-19	0.17420126796104357
20-21	0.20539630734152226
22-23	0.16990724327380496
24-25	0.19418694778697698
26-27	0.192767674871802
28-29	0.22096486227839068
30-31	0.2553387833413276
32-33	0.22395547306393795
34-35	0.15107291239436554
36-37	0.14530168528521015
38-39	0.11247013733927458
40-41	0.10328831052069357
42-43	0.11926237200475485
44-45	0.13407060216563668
46-47	0.20087056462731628
48-49	0.20574388438197327
50-51	0.21117477563902037
52-53	0.1385239329964153
54-55	0.08282471226414001
56-57	0.12092060413523992
58-59	0.14091352514951605
60-61	0.11740862778901609
62-63	0.1385384153731008
64-65	0.11386044550107866
66-67	0.11737966303564519
68-69	0.10290452753852891
70-71	0.12872660516870232
72-73	0.15352043405420812
74-75	0.19180459682221898
76-77	0.17226787067353477
78-79	0.19709066431241154
80-81	0.1011304363945602
82-83	0.14148557902859168
84-85	0.17314405446300507
86-87	0.18719920103624302
88-89	0.16784350459612707
90-91	0.1714423752024636
92-93	0.1213405930591182
94-95	0.35999567845879704
96-97	0.35894570614910126
98-99	0.40576722997319026
100	0.43878704881603675
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6904944.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.24513947212964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.07562370094287	28.298618704141848
2	11.91242578820857	8.635350682900269
3	3.8282123808288193	4.162622750162221
4	1.7745077329280001	2.5726912109739173
5	1.0061136708983227	1.8233365163263027
6	0.635624055038756	1.382296951603219
7	0.4225620181074496	1.0721073497350357
8	0.3066016383024632	0.8890255314124977
9	0.24342754556491167	0.7940758806322575
>10	1.4902800013841198	10.2519845357032
>50	0.14861923803927476	3.771061818817704
>100	0.1260382028695883	9.241908196802129
>500	0.017093578457786047	4.274651109818599
>1k	0.0107387804081011	7.320427708497025
>5k	7.641828754818759E-4	1.9703567770246306
>10k+	0.0013674851455991463	13.53948427544886
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	116470	1.6867624125554097	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	102224	1.4804464742943608	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	55911	0.809724162860698	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	47400	0.6864646548907566	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	44341	0.6421630646099374	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	42618	0.6172099295808916	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	38563	0.5584838921213553	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	37220	0.5390340602327839	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	37132	0.5377596110844636	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	36864	0.5338783341327605	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	34917	0.5056811467261718	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	30169	0.4369188222236125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22688	0.32857616223969377	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	22289	0.3227976939421956	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	18548	0.2686191227618935	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	16591	0.24027711158845028	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15690	0.2272284901948517	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	15641	0.22651885373726421	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	15235	0.22063900880296786	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	15216	0.2203638436459441	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	14520	0.21028410947286466	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	13700	0.1984085605907883	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	13012	0.1884446854311925	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	12578	0.1821593339497033	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	12555	0.18182623928593775	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	11819	0.17116721004543992	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11240	0.16278191394455915	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	11074	0.16037783941477296	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	10583	0.1532669924622126	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	10547	0.15274562690153606	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	10434	0.15110911833607918	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	10414	0.15081947080237001	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	10366	0.15012431672146798	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	10202	0.1477492069450527	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	9538	0.13813290882590792	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	9250	0.13396198434049575	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	8736	0.1265180427241698	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	8528	0.12350570837359434	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	7698	0.11148533572466338	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	7547	0.10929849684515906	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	7515	0.10883506079122439	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	7504	0.10867575464768432	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	7489	0.10845851899740244	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	7361	0.10660477478166368	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	7091	0.10269453307658975	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.30341390169131E-4	0.0	0.0	0.0	0.0
2	2.7516515702372097E-4	0.0	0.0	0.0	0.0
3	3.0412991039463895E-4	0.0	0.0	0.0	0.0
4	3.6205941713647496E-4	0.0	0.0	0.0	0.0
5	0.0014047905384895229	0.0	0.0	4.3447130056377E-5	0.0
6	0.0032006052474864385	0.0	0.0	4.3447130056377E-5	1.4482376685458998E-5
7	0.0035626646646229137	0.0	0.0	7.241188342729499E-5	2.8964753370917997E-5
8	0.0038957593283884704	0.0	0.0	1.01376636798213E-4	2.8964753370917997E-5
9	0.004228853992154028	0.0	0.0	1.1585901348367199E-4	8.6894260112754E-5
10-11	0.006900852490621213	0.0	0.0	2.244768386246145E-4	9.413544845548349E-5
12-13	0.013707569532786942	0.0	0.0	2.679239686809915E-4	1.01376636798213E-4
14-15	0.021028410947286465	0.0	0.0	3.9826535885012244E-4	1.30341390169131E-4
16-17	0.023512138548842685	0.0	0.0	6.299833858174665E-4	1.4482376685458998E-4
18-19	0.02477210532047762	0.0	0.0	8.617014127848103E-4	1.4482376685458998E-4
20-21	0.03061574431306032	0.0	0.0	0.0015134083636304653	1.4482376685458998E-4
22-23	0.032766377250850985	0.0	0.0	0.003823347444961176	1.59306143540049E-4
24-25	0.0459018929045623	1.4482376685458998E-5	0.0	0.009420786033891078	1.73788520225508E-4
26-27	0.08489569213016065	2.8964753370917997E-5	0.0	0.0202029154762153	1.8102970856823748E-4
28-29	0.09478715540632915	2.8964753370917997E-5	0.0	0.058907067168104475	1.8827089691096698E-4
30-31	0.0968364117073216	2.8964753370917997E-5	0.0	0.15173186053355392	1.8827089691096698E-4
32-33	0.09835706125929479	2.8964753370917997E-5	0.0	0.2910668066243549	2.02753273596426E-4
34-35	0.09953737495915971	2.8964753370917997E-5	0.0	0.47435576595552403	2.2447683862461448E-4
36-37	0.10158663126015215	2.8964753370917997E-5	0.0	0.739723595151532	2.3171802696734397E-4
38-39	0.10367933469120097	2.8964753370917997E-5	0.0	1.112463765093533	2.389592153100735E-4
40-41	0.10584445000567709	2.8964753370917997E-5	0.0	1.6320552346260881	2.60682780338262E-4
42-43	0.11086259352718864	2.8964753370917997E-5	0.0	2.005193669926939	2.60682780338262E-4
44-45	0.11667726776640042	2.8964753370917997E-5	0.0	2.3915762387066426	2.679239686809915E-4
46-47	0.11927685438144031	2.8964753370917997E-5	0.0	2.8435060443647338	2.7516515702372097E-4
48-49	0.12073233323832894	2.8964753370917997E-5	0.0	3.242191392138734	2.7516515702372097E-4
50-51	0.12312192539142967	2.8964753370917997E-5	0.0	3.668921862364126	2.7516515702372097E-4
52-53	0.12409224462935543	2.8964753370917997E-5	0.0	4.1999906154199085	2.8240634536645046E-4
54-55	0.1267642431278226	2.8964753370917997E-5	0.0	4.663028403995746	2.8964753370917996E-4
56-57	0.13401991384723758	2.8964753370917997E-5	0.0	5.142083701185701	2.8964753370917996E-4
58-59	0.13661950046227744	4.3447130056377E-5	0.0	5.635838610711398	2.8964753370917996E-4
60-61	0.13845152111298803	5.792950674183599E-5	0.0	6.065921751139474	2.8964753370917996E-4
62-63	0.13966804075456657	5.792950674183599E-5	0.0	6.505056376995961	2.8964753370917996E-4
64-65	0.1405804304857505	5.792950674183599E-5	0.0	6.995386204435547	2.8964753370917996E-4
66-67	0.14199246221258277	5.792950674183599E-5	0.0	7.502660412597119	2.9688872205190945E-4
68-69	0.14346966463449956	5.792950674183599E-5	0.0	8.099623979571739	3.0412991039463895E-4
70-71	0.14543202667537927	5.792950674183599E-5	0.0	8.826298953329673	3.0412991039463895E-4
72-73	0.15026189929997985	5.792950674183599E-5	0.0	9.405282649649294	3.0412991039463895E-4
74-75	0.15510625430126587	5.792950674183599E-5	0.0	9.961529014572747	3.113710987373685E-4
76-77	0.1567572452434082	7.241188342729499E-5	0.0	10.566537541796139	3.18612287080098E-4
78-79	0.15769859972796302	7.241188342729499E-5	0.0	11.142646486343699	3.18612287080098E-4
80-81	0.15941476136518992	7.241188342729499E-5	0.0	11.793035540910976	3.18612287080098E-4
82-83	0.1604430101098575	7.241188342729499E-5	0.0	12.562230772617418	3.18612287080098E-4
84-85	0.16401291596282316	7.241188342729499E-5	0.0	13.261729566525087	3.18612287080098E-4
86-87	0.17048653834122335	8.6894260112754E-5	0.0	13.944349150405854	3.18612287080098E-4
88	0.17193477600976922	8.6894260112754E-5	0.0	14.49659837936412	3.18612287080098E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	7270	0.0	27.716715	1
ATCTACA	8325	0.0	24.01523	2
TCTACAC	9710	0.0	22.57363	3
TACACTC	9630	0.0	22.471033	5
CTACACT	11270	0.0	19.994593	4
AGTACTC	2850	0.0	19.312235	5
AGTACTT	28480	0.0	18.52344	12-13
CACTCTT	11855	0.0	18.12149	7
ACTCTTT	14395	0.0	17.111925	8
GAGTACT	27915	0.0	16.86962	12-13
ACACTCT	13190	0.0	16.215961	6
CATGGGG	13540	0.0	16.086563	4
ATACCGA	765	0.0	15.976735	6
TACTTTT	33330	0.0	15.971543	14-15
TATACCG	865	0.0	15.771513	5
CGTTAAC	510	3.6379788E-12	15.693186	1
TCTAACG	640	0.0	15.435524	2
CATGGGT	7475	0.0	15.356052	4
AAGTACT	3805	0.0	14.960003	4
GTACTTT	31795	0.0	14.947185	14-15
>>END_MODULE