FastQCFastQC Report
Fri 27 May 2016
ERR765392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13101640
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171130.13061723570484304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA245400.041.7135241
GGTATCA135750.035.247581
ATCAACG289850.033.2899863
CAACGCA292450.032.9298025
TATCAAC334250.030.5114942
AACGCAG341050.029.3386736
TCAACGC359350.026.982214
ACGCAGA360400.026.5388017
CGCAGAG364150.026.123678
GCAGAGT380000.024.1078629
GTACATG488050.021.5804211
AGAGTAC348150.021.2569710-11
TACATGG487850.020.7990532
AGTACAT377500.019.48300612-13
CGTTAAC18250.019.297161
ACATGGG520550.018.8971653
CAGAGTA364650.018.8206310-11
GTACTAG53700.018.2754041
CATGGGG341700.018.2485434
GAGTACA374400.018.23936712-13
Produced by FastQC (version 0.11.5)