FastQCFastQC Report
Fri 27 May 2016
ERR765386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13740944
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA893660.6503628862762267No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT811460.5905416687528892No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG364160.2650181821569173No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA319610.23259682886415953No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG317840.2313087077569052No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC310010.22561040929939022No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT293020.21324590217382447No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG274010.19941133593150512No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC264050.19216292563305695No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC262300.19088935956656253No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC197990.14408762600298786No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT167780.12210223693510433No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA139830.10176156747309356No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG139630.1016160170654942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138370.10069904949761821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG67550.022.9569111
GTACAAG54150.022.6499351
GTGATCG50500.022.6080918
TATTCGC11150.022.333159
GTACTAG66400.021.5144581
CGTTAAC22100.021.2633931
AATACGG33700.020.7755625
TGATCGC56900.020.395479
TAATACG38750.020.3721644
GTACATA38650.018.8454741
TAGGCAT92900.018.7146825
CTATTGA75150.018.3184059
ATAAGGT68200.018.1211933
GGTCGCC80250.017.91613894
AGGGCTA82350.017.8027635
TAAGGTG67350.017.7910754
GGGCTAT87400.017.7399146
TAGTACT81450.017.7111554
CATGGGG359600.017.6014124
ACGTTAA17100.017.5846148
Produced by FastQC (version 0.11.5)