##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765386_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13740944
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.714316570972127	33.0	33.0	33.0	27.0	33.0
2	31.594709504674498	33.0	33.0	33.0	27.0	33.0
3	31.659130115077975	33.0	33.0	33.0	27.0	33.0
4	35.368822695151074	37.0	37.0	37.0	33.0	37.0
5	35.44554748203617	37.0	37.0	37.0	33.0	37.0
6	35.42863779955729	37.0	37.0	37.0	33.0	37.0
7	35.37151450438922	37.0	37.0	37.0	33.0	37.0
8	35.355798844679086	37.0	37.0	37.0	33.0	37.0
9	35.33888363128472	37.0	37.0	37.0	33.0	37.0
10-11	35.314502446120144	37.0	37.0	37.0	33.0	37.0
12-13	35.27717575299047	37.0	37.0	37.0	33.0	37.0
14-15	36.775523646701416	38.5	37.0	40.0	33.0	40.0
16-17	36.791084149677054	37.0	37.0	40.0	33.0	40.0
18-19	36.760557134939205	38.5	37.0	40.0	33.0	40.0
20-21	36.85427278504301	40.0	37.0	40.0	33.0	40.0
22-23	36.85897300796801	40.0	37.0	40.0	33.0	40.0
24-25	36.847998470847415	38.5	37.0	40.0	33.0	40.0
26-27	36.80241313842775	37.0	37.0	40.0	33.0	40.0
28-29	36.749826685852156	37.0	37.0	40.0	33.0	40.0
30-31	36.66296220259686	37.0	37.0	40.0	33.0	40.0
32-33	36.530060853169914	37.0	37.0	40.0	33.0	40.0
34-35	36.43587998757582	37.0	37.0	40.0	33.0	40.0
36-37	36.363886862503776	37.0	37.0	40.0	33.0	40.0
38-39	36.251349252278445	37.0	37.0	40.0	33.0	40.0
40-41	36.13533917320382	37.0	37.0	40.0	30.0	40.0
42-43	35.984241512082434	37.0	37.0	40.0	27.0	40.0
44-45	35.801110753380556	37.0	37.0	40.0	27.0	40.0
46-47	35.60940794897353	37.0	37.0	40.0	27.0	40.0
48-49	35.35971855354334	37.0	33.0	40.0	27.0	40.0
50-51	35.26050895775428	37.0	33.0	40.0	27.0	40.0
52-53	35.107592717065145	37.0	33.0	40.0	27.0	40.0
54-55	34.942338059161	37.0	33.0	40.0	27.0	40.0
56-57	34.76091871853928	37.0	33.0	40.0	27.0	40.0
58-59	34.57203071346481	37.0	33.0	40.0	27.0	40.0
60-61	34.35842861305599	37.0	33.0	37.0	27.0	40.0
62-63	34.11074894126634	37.0	33.0	37.0	27.0	40.0
64-65	33.92326200441542	37.0	33.0	37.0	27.0	40.0
66-67	33.73303155154406	37.0	33.0	37.0	27.0	40.0
68-69	33.52292473501093	37.0	33.0	37.0	27.0	40.0
70-71	33.308056164117986	37.0	33.0	37.0	27.0	38.5
72-73	33.0668418414339	37.0	33.0	37.0	24.5	37.0
74-75	32.83409043803687	37.0	33.0	37.0	22.0	37.0
76-77	32.63494564128928	37.0	33.0	37.0	22.0	37.0
78-79	32.47744579993922	35.0	33.0	37.0	22.0	37.0
80-81	32.33057495904212	33.0	33.0	37.0	22.0	37.0
82-83	32.205471254376704	33.0	33.0	37.0	22.0	37.0
84-85	32.059109657968186	33.0	33.0	37.0	22.0	37.0
86-87	31.938735104371286	33.0	33.0	37.0	22.0	37.0
88-89	31.82663047022097	33.0	33.0	37.0	22.0	37.0
90-91	31.701467380989254	33.0	33.0	37.0	22.0	37.0
92-93	31.582226264803932	33.0	33.0	37.0	22.0	37.0
94-95	31.48811348041299	33.0	33.0	37.0	22.0	37.0
96-97	31.41515310010724	33.0	33.0	37.0	22.0	37.0
98-99	31.308497873217444	33.0	33.0	37.0	18.5	37.0
100	31.232064623798774	33.0	33.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	15.0
7	159.0
8	889.0
9	1906.0
10	3248.0
11	6488.0
12	14257.0
13	27443.0
14	38605.0
15	42305.0
16	47979.0
17	55015.0
18	65132.0
19	75626.0
20	86985.0
21	103246.0
22	117311.0
23	116401.0
24	117235.0
25	127909.0
26	146733.0
27	173954.0
28	213356.0
29	268434.0
30	340790.0
31	438513.0
32	573868.0
33	752605.0
34	988123.0
35	1354302.0
36	2191043.0
37	3599331.0
38	1650102.0
39	1634.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.8201154901555	19.757197426470615	12.769259089447042	24.65342799392684
2	15.4134619030599	21.82606042040618	37.6698905895324	25.09058708700152
3	18.202709368963347	27.586803693093447	29.348692644395285	24.861794293547916
4	12.81544560116282	18.09455529837405	37.36749661800337	31.72250248245976
5	13.271511656565183	38.47302501905973	33.817701751267926	14.437761573107155
6	30.095465056840343	37.621796581079145	18.21246051217442	14.070277849906091
7	25.853645863049874	32.55402248928458	23.25113907748987	18.341192570175675
8	26.469855346182914	33.259432539714886	20.85904723867589	19.411664875426318
9	27.073882260199884	14.842466427342982	20.24804118261453	37.83561012984261
10-11	24.91550434962838	26.27332226956168	27.085220636951874	21.725952743858066
12-13	26.077345923249524	24.215665241048942	26.854293999014917	22.85269483668662
14-15	24.314988839194747	24.337607372535686	24.91589005820852	26.431513730061052
16-17	23.330669629405048	26.721730536916038	26.341180264759384	23.606419568919527
18-19	23.618318509613374	26.550013489120605	27.878657300139594	21.953010701126427
20-21	24.634844862829667	25.771872411008562	26.736854837160262	22.856427889001512
22-23	24.0167101890764	25.81629308572096	25.86887222744553	24.29812449775712
24-25	23.60889806833116	26.421731745711625	26.083067968347034	23.88630221761018
26-27	24.069067243022747	25.83442702853258	25.988086564328384	24.108419164116288
28-29	23.546644007944238	25.805950404127138	26.808213801438896	23.83919178648973
30-31	24.42021599100977	25.698219878831935	26.525707788211633	23.355856341946666
32-33	23.27475194043972	26.208192325990744	26.251026314371124	24.26602941919841
34-35	23.45498243850967	26.23512078981234	26.957101459380933	23.352795312297054
36-37	24.183835647107923	25.808219828165353	26.33568095312752	23.6722635715992
38-39	24.410631818529893	25.28059172224757	26.337763209193348	23.971013250029188
40-41	23.737095984043	25.628967325289725	26.70622309413054	23.927713596536744
42-43	24.676453470005598	25.3384136568226	26.187714199449076	23.797418673722724
44-45	24.310218992181625	25.65225879018908	26.222301706725908	23.81522051090339
46-47	23.67402467791095	25.47513134574515	26.895835958282937	23.955008018060965
48-49	24.336615985194605	25.73290304140146	26.474104512872053	23.45637646053188
50-51	23.3414650975113	26.216005402220276	25.882711759698434	24.559817740569986
52-53	23.038252624729257	26.329988457884816	25.305891894738792	25.32586702264713
54-55	23.419476043393786	26.12344920155322	25.309995902414723	25.147078852638273
56-57	24.106052962502986	25.129006456855326	25.352606488327677	25.412334092314016
58-59	23.500351127071315	25.205894101705734	26.444104704578685	24.84965006664427
60-61	23.711419785661473	25.606687622930497	25.80753263795184	24.87435995345619
62-63	22.965179441993218	25.502982419936522	25.85588664438541	25.675951493684856
64-65	22.573560620394005	25.556107376283133	26.140959711713034	25.729372291609824
66-67	23.852586077197895	25.196271594861336	25.665859678720242	25.28528264922053
68-69	23.718613475192406	24.78800416322367	25.710922916205643	25.782459445378286
70-71	22.96384454122181	25.091905423042242	25.948185210879913	25.99606482485603
72-73	23.98416601844144	24.72080461809299	26.01744067033312	25.27758869313245
74-75	23.936322833435668	24.748596942214824	26.160200671951046	25.15487955239847
76-77	23.15008458949984	25.09644731378756	26.475334400542653	25.278133696169945
78-79	23.305003423912044	25.171826964386295	26.34076050657741	25.182409105124254
80-81	22.79849510281806	25.16453331226682	26.648994715044054	25.387976869871064
82-83	23.080066132417958	25.049483600656608	26.203616993219374	25.666833273706064
84-85	23.237784195825924	25.234615991604148	25.70046566044846	25.827134152121474
86-87	22.841218855854	24.69441708033056	26.30095983996069	26.16340422385475
88-89	23.15861192541418	24.144090270216655	26.469240594553924	26.22805720981524
90-91	23.356148185959224	24.479635672649895	26.24101041352707	25.923205727863806
92-93	23.015140374344927	24.60221589369422	26.36793488344709	26.014708848513763
94-95	22.19038082066394	24.751070676376212	26.85755569312943	26.200992809830414
96-97	22.930290081860207	24.556534137676117	26.201106898011766	26.31206888245191
98-99	22.842725817526567	24.592380219763424	26.09560949836198	26.469284464348032
100	22.856518016439548	24.60337924456967	26.014411987210206	26.525690751780573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	582.0
1	537.0
2	669.5
3	832.0
4	680.0
5	573.0
6	769.5
7	1492.5
8	2316.5
9	2177.0
10	1397.0
11	1060.0
12	1153.5
13	1329.5
14	1554.5
15	1910.5
16	2349.5
17	2875.0
18	3567.5
19	4461.0
20	5583.5
21	6999.0
22	8813.0
23	11324.0
24	14687.0
25	19400.0
26	26230.0
27	34495.5
28	46201.5
29	65296.5
30	85975.0
31	105500.5
32	130490.5
33	156247.5
34	183441.0
35	207251.0
36	230343.5
37	266064.0
38	285827.0
39	294741.5
40	300470.5
41	302232.5
42	313709.0
43	344809.5
44	395245.0
45	451776.0
46	517702.5
47	586380.0
48	682544.0
49	753139.5
50	827793.5
51	815524.5
52	665892.0
53	582134.0
54	545239.5
55	498623.5
56	465333.0
57	414436.5
58	368135.5
59	341747.5
60	291777.5
61	230266.5
62	180628.0
63	141346.5
64	105205.5
65	80443.0
66	63745.0
67	47466.0
68	35791.5
69	28794.5
70	24785.0
71	21897.0
72	18933.0
73	19312.5
74	15667.5
75	11481.0
76	8960.0
77	6653.0
78	5097.5
79	3287.5
80	2290.5
81	1761.5
82	1463.5
83	1281.5
84	989.0
85	653.5
86	457.0
87	263.5
88	133.0
89	91.0
90	65.5
91	44.5
92	33.0
93	24.0
94	24.0
95	22.5
96	9.5
97	3.0
98	4.5
99	4.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017880867573581552
2	0.0061931698433528295
3	0.006680763708810691
4	0.003929861005182759
5	0.047267494867892626
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.820698199483238E-4
18-19	0.0017538824115723055
20-21	5.239814673577012E-4
22-23	0.005127013107687507
24-25	0.007736004163906061
26-27	0.006018509354233596
28-29	0.023200734971338215
30-31	0.021239443228936818
32-33	0.01349616154465079
34-35	0.014071085654668268
36-37	0.013987394170298633
38-39	0.021919891384463833
40-41	0.031151425986453332
42-43	0.018776002580317624
44-45	0.017589766758382832
46-47	0.018812390182217465
48-49	0.013823649961749352
50-51	0.010246748694995044
52-53	0.028094867426866742
54-55	0.02608991056218554
56-57	0.01384912128307924
58-59	0.016942067444565674
60-61	0.0181210257461205
62-63	0.021388632396726164
64-65	0.011487565919779601
66-67	0.016017822356309726
68-69	0.008081686381954544
70-71	0.00928611600483926
72-73	0.0109635844524219
74-75	0.003700619113213765
76-77	0.007706894082386188
78-79	0.0059166240689140425
80-81	0.0024561631282392243
82-83	2.03770570639106E-4
84-85	0.0025653259339387456
86-87	0.002328806521589783
88-89	0.0011280156588950512
90-91	8.332760835063443E-4
92-93	0.001218984663644652
94-95	0.003256690370035712
96-97	0.0014700591167535505
98-99	0.0018848777784117308
100	0.0021759785936104536
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3740944E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.35660495002969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.67422152188378	21.150728179406826
2	15.108661903407302	9.172953614505982
3	6.0527558275252975	5.512233525455266
4	2.917979858635039	3.543198472829094
5	1.6368645244484221	2.4844824862699486
6	0.9565257853495821	1.7422125234224497
7	0.6674200501562102	1.4182424758824752
8	0.4648945077377985	1.1290095131867897
9	0.3294712694081001	0.9001466251025847
>10	1.8268862516292055	10.172741267250924
>50	0.15618945229132464	3.301504057018118
>100	0.1491607140409297	9.689257478349449
>500	0.029337670421763395	6.260495194189517
>1k	0.02746080320508263	15.893304271750544
>5k	0.0015671210102099838	3.2140841300786436
>10k+	6.02738850080763E-4	4.415406185301523
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	89366	0.6503628862762267	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	81146	0.5905416687528892	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	36416	0.2650181821569173	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	31961	0.23259682886415953	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	31784	0.2313087077569052	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	31001	0.22561040929939022	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	29302	0.21324590217382447	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	27401	0.19941133593150512	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	26405	0.19216292563305695	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	26230	0.19088935956656253	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	19799	0.14408762600298786	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	16778	0.12210223693510433	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13983	0.10176156747309356	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	13963	0.1016160170654942	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13837	0.10069904949761821	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	7.277520379968072E-6	0.0
12-13	1.0916280569952108E-5	0.0	0.0	1.819380094992018E-5	0.0
14-15	2.1832561139904217E-5	1.0916280569952108E-5	0.0	5.094264265977651E-5	1.4555040759936144E-5
16-17	2.5471321329888252E-5	2.1832561139904217E-5	0.0	9.460776493958494E-5	2.1832561139904217E-5
18-19	2.9110081519872288E-5	2.1832561139904217E-5	0.0	1.1644032607948916E-4	2.9110081519872288E-5
20-21	2.9110081519872288E-5	2.1832561139904217E-5	0.0	2.2560313177901023E-4	2.9110081519872288E-5
22-23	3.274884170985633E-5	2.9110081519872288E-5	0.0	7.677784000866316E-4	4.00263620898244E-5
24-25	3.638760189984036E-5	3.274884170985633E-5	0.0	0.002532577092228889	5.0942642659776505E-5
26-27	5.458140284976054E-5	3.638760189984036E-5	0.0	0.004923242537048401	5.0942642659776505E-5
28-29	5.8220163039744576E-5	3.638760189984036E-5	0.0	0.017214974458814473	5.0942642659776505E-5
30-31	5.8220163039744576E-5	3.638760189984036E-5	0.0	0.06529755160926352	5.0942642659776505E-5
32-33	5.8220163039744576E-5	4.3665122279808433E-5	0.0	0.15188912785031364	5.0942642659776505E-5
34-35	6.185892322972862E-5	5.458140284976054E-5	0.0	0.25935991006149217	5.0942642659776505E-5
36-37	6.549768341971265E-5	5.8220163039744576E-5	0.0	0.43472995741777276	5.0942642659776505E-5
38-39	6.549768341971265E-5	5.8220163039744576E-5	0.0	0.725510561719777	5.0942642659776505E-5
40-41	8.00527241796488E-5	6.549768341971265E-5	0.0	1.117110294605669	5.0942642659776505E-5
42-43	8.00527241796488E-5	6.549768341971265E-5	0.0	1.4277112256625164	5.0942642659776505E-5
44-45	9.460776493958494E-5	7.277520379968072E-5	0.0	1.7591622526079722	5.0942642659776505E-5
46-47	1.0916280569952108E-4	7.641396398966476E-5	0.0	2.1286128522174312	5.0942642659776505E-5
48-49	1.1644032607948915E-4	8.00527241796488E-5	0.0	2.528705451386746	5.0942642659776505E-5
50-51	1.1644032607948915E-4	8.00527241796488E-5	0.0	2.9589888438523584	5.0942642659776505E-5
52-53	1.2735660664944126E-4	1.0188528531955301E-4	0.0	3.3828898509447383	5.822016303974458E-5
54-55	1.309953668394253E-4	1.0552404550953704E-4	0.0	3.7795292666937588	6.549768341971265E-5
56-57	1.309953668394253E-4	1.0916280569952108E-4	0.0	4.219298906974659	6.549768341971265E-5
58-59	1.3827288721939338E-4	1.0916280569952108E-4	0.0	4.7121216708255265	6.549768341971265E-5
60-61	1.3827288721939338E-4	1.0916280569952108E-4	0.0	5.212967900895309	6.549768341971265E-5
62-63	1.3827288721939338E-4	1.1280156588950512E-4	0.0	5.721804848342297	7.277520379968072E-5
64-65	1.3827288721939338E-4	1.1644032607948915E-4	0.0	6.234848202568907	7.277520379968072E-5
66-67	1.3827288721939338E-4	1.1644032607948915E-4	0.0	6.773817723149152	7.277520379968072E-5
68-69	1.4555040759936145E-4	1.1644032607948915E-4	0.0	7.412864065234528	7.277520379968072E-5
70-71	1.5282792797932952E-4	1.2007908626947319E-4	0.0	8.159741426789893	7.277520379968072E-5
72-73	1.5282792797932952E-4	1.2735660664944126E-4	0.0	8.83489154748029	8.00527241796488E-5
74-75	1.601054483592976E-4	1.309953668394253E-4	0.0	9.483227644330695	8.00527241796488E-5
76-77	1.7102172892924968E-4	1.309953668394253E-4	0.0	10.181298315457802	8.00527241796488E-5
78-79	1.8921552987916988E-4	1.309953668394253E-4	0.0	10.888898899522477	8.00527241796488E-5
80-81	1.8921552987916988E-4	1.419116474093774E-4	0.0	11.670755662784158	8.733024455961687E-5
82-83	1.8921552987916988E-4	1.4555040759936145E-4	0.0	12.536857001964348	1.0188528531955301E-4
84-85	1.8921552987916988E-4	1.4555040759936145E-4	0.0	13.349308460903414	1.0188528531955301E-4
86-87	1.8921552987916988E-4	1.4555040759936145E-4	0.0	14.156705681938591	1.0188528531955301E-4
88	1.8921552987916988E-4	1.601054483592976E-4	0.0	14.782492381891666	1.0916280569952108E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAAG	6755	0.0	22.956911	1
GTACAAG	5415	0.0	22.649935	1
GTGATCG	5050	0.0	22.608091	8
TATTCGC	1115	0.0	22.33315	9
GTACTAG	6640	0.0	21.514458	1
CGTTAAC	2210	0.0	21.263393	1
AATACGG	3370	0.0	20.775562	5
TGATCGC	5690	0.0	20.39547	9
TAATACG	3875	0.0	20.372164	4
GTACATA	3865	0.0	18.845474	1
TAGGCAT	9290	0.0	18.714682	5
CTATTGA	7515	0.0	18.318405	9
ATAAGGT	6820	0.0	18.121193	3
GGTCGCC	8025	0.0	17.916138	94
AGGGCTA	8235	0.0	17.802763	5
TAAGGTG	6735	0.0	17.791075	4
GGGCTAT	8740	0.0	17.739914	6
TAGTACT	8145	0.0	17.711155	4
CATGGGG	35960	0.0	17.601412	4
ACGTTAA	1710	0.0	17.584614	8
>>END_MODULE