FastQCFastQC Report
Fri 27 May 2016
ERR765383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6833944
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA419840.6143450985258293No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT380140.5562527290244111No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG162600.23792995669850384No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG151190.2212338877813456No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT125450.1835689610567485No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC122970.179940017067743No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG121930.1784182018465472No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC109430.16012715351486637No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA106290.15553244217394815No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC106030.15515198836864919No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC89710.13127119566680676No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA89040.13029079547622865No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80010.11707734216142246No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT79120.11577501952020677No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT77060.1127606547551458No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC76860.11246799798183889No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA76340.11170709037124098No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC72700.10638073709705552No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA69460.10163969736948386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA17250.042.3205761
TACATGA24300.032.529972
GTACAAA38650.029.1143361
GTACAAG37500.028.8912051
ACGTTAA8550.026.102068
GTACTAG38500.025.4837151
CTATTGA41000.024.7224439
TAGGCAT50800.024.3463295
TACAAGA23450.024.1912332
GTGATCG28900.024.1319898
AGGGCTA41550.023.94745
TATTCGC6150.023.4371789
CGTTAAC10800.023.2493611
GTATAAG39350.022.92441
TAGTACT45250.022.8112964
GGGCTAT45450.022.6081186
ATAAGGT32550.022.5708073
TGATCGC31850.022.1897759
TACATAA18500.021.8669662
ATTTAGG47100.021.817871
Produced by FastQC (version 0.11.5)