##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765383_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6833944 Sequences flagged as poor quality 0 Sequence length 99 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.87711225026134 33.0 33.0 33.0 27.0 33.0 2 30.77382328564589 33.0 33.0 33.0 27.0 33.0 3 30.774022292251736 33.0 33.0 33.0 27.0 33.0 4 34.15675443053089 37.0 37.0 37.0 27.0 37.0 5 34.46712132847445 37.0 37.0 37.0 27.0 37.0 6 34.261578965235884 37.0 37.0 37.0 27.0 37.0 7 34.18201831914338 37.0 37.0 37.0 27.0 37.0 8 34.037003961402085 37.0 37.0 37.0 27.0 37.0 9 33.768144280959866 37.0 37.0 37.0 27.0 37.0 10-11 33.90777368090812 37.0 37.0 37.0 27.0 37.0 12-13 33.83328433771187 37.0 37.0 37.0 27.0 37.0 14-15 35.163024601899 37.0 37.0 40.0 27.0 40.0 16-17 35.31619208468784 37.0 37.0 40.0 27.0 40.0 18-19 35.38659754894099 37.0 37.0 40.0 27.0 40.0 20-21 35.46025069857171 37.0 37.0 40.0 27.0 40.0 22-23 35.5579938904972 37.0 37.0 40.0 27.0 40.0 24-25 35.708127107860406 37.0 37.0 40.0 27.0 40.0 26-27 35.70370169846285 37.0 33.0 40.0 27.0 40.0 28-29 35.722756873629635 37.0 33.0 40.0 27.0 40.0 30-31 35.62570903419753 37.0 33.0 40.0 27.0 40.0 32-33 35.490225922249294 37.0 33.0 40.0 27.0 40.0 34-35 35.31832050423591 37.0 33.0 40.0 27.0 40.0 36-37 35.0857476297728 37.0 33.0 40.0 27.0 40.0 38-39 34.74040597932907 37.0 33.0 40.0 24.5 40.0 40-41 34.79632090927289 37.0 33.0 40.0 27.0 40.0 42-43 34.679718622218736 37.0 33.0 40.0 27.0 40.0 44-45 34.57185930993874 37.0 33.0 40.0 22.0 40.0 46-47 34.46900172140714 37.0 33.0 40.0 22.0 40.0 48-49 34.32600479313263 37.0 33.0 40.0 22.0 40.0 50-51 34.13620890074604 37.0 33.0 40.0 22.0 40.0 52-53 33.98747846046148 37.0 33.0 40.0 22.0 40.0 54-55 33.78072771740594 37.0 33.0 38.5 22.0 40.0 56-57 33.55279791581552 37.0 33.0 37.0 22.0 40.0 58-59 33.321791779388306 37.0 33.0 37.0 22.0 40.0 60-61 33.09408637237882 37.0 33.0 37.0 22.0 40.0 62-63 32.82967112402443 37.0 33.0 37.0 22.0 40.0 64-65 32.61709343828395 37.0 33.0 37.0 22.0 40.0 66-67 32.39952427178215 37.0 33.0 37.0 22.0 40.0 68-69 32.18277842194785 35.0 33.0 37.0 22.0 38.5 70-71 31.920821856310205 33.0 30.0 37.0 22.0 37.0 72-73 31.562891574762684 33.0 27.0 37.0 22.0 37.0 74-75 31.30154161345191 33.0 27.0 37.0 22.0 37.0 76-77 31.0940548386115 33.0 27.0 37.0 15.0 37.0 78-79 30.832862692465728 33.0 27.0 37.0 15.0 37.0 80-81 30.62082027596363 33.0 27.0 37.0 15.0 37.0 82-83 30.47481205582018 33.0 27.0 37.0 15.0 37.0 84-85 30.302010376438552 33.0 27.0 37.0 15.0 37.0 86-87 30.001904244459716 33.0 27.0 37.0 15.0 37.0 88-89 29.82604605773767 33.0 27.0 37.0 15.0 37.0 90-91 29.64824916622085 33.0 27.0 37.0 15.0 37.0 92-93 29.345268852071364 33.0 27.0 37.0 15.0 37.0 94-95 29.18966807454085 33.0 27.0 37.0 15.0 37.0 96-97 28.988407206731573 33.0 27.0 37.0 15.0 37.0 98-99 27.925780998498084 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.494401283491289 1101 2 0.5395005044490055 1101 3 0.5391544194693907 1101 4 0.6309304410776093 1101 5 0.49395588046346006 1101 6 0.6470389469085944 1101 7 0.5405106087361915 1101 8 0.5376837851650151 1101 9 0.5799827002285767 1101 10-11 0.48271103162352347 1101 12-13 0.6450755531345749 1101 14-15 0.8046384671617659 1101 16-17 0.6866987116020837 1101 18-19 0.6818389031099699 1101 20-21 0.6328198708772987 1101 22-23 0.650342948084905 1101 24-25 0.5604465534099035 1101 26-27 0.6315261811192272 1101 28-29 0.5840458767084513 1101 30-31 0.7906688362192327 1101 32-33 0.8611813584116632 1101 34-35 0.9202291250562311 1101 36-37 1.0203938399827877 1101 38-39 1.0020902094172897 1101 40-41 0.7913492813750551 1101 42-43 0.7273977383862231 1101 44-45 0.636250173811554 1101 46-47 0.6765190161002224 1101 48-49 0.603160132117651 1101 50-51 0.5544774570747109 1101 52-53 0.639595762919825 1101 54-55 0.44477119313870617 1101 56-57 0.6111117563953954 1101 58-59 0.5165691031610962 1101 60-61 0.3275153199299581 1101 62-63 0.5016899347062491 1101 64-65 0.5760413477768367 1101 66-67 0.553753367527122 1101 68-69 0.6355649571203159 1101 70-71 0.6684220247376906 1101 72-73 0.44976956899526854 1101 74-75 0.47734412618182276 1101 76-77 0.49045341774950657 1101 78-79 0.5653772906820365 1101 80-81 0.5332362660864867 1101 82-83 0.5874103455147122 1101 84-85 0.6156558784005561 1101 86-87 0.5521396264880813 1101 88-89 0.3656073486932492 1101 90-91 0.30483961183929864 1101 92-93 0.3921350945645905 1101 94-95 0.5211694530393025 1101 96-97 0.35802093110999067 1101 98-99 0.3103345486201263 1102 1 0.39620270310411243 1102 2 0.47063228560515213 1102 3 0.3825114949931958 1102 4 0.5123210341246391 1102 5 0.31412569533546275 1102 6 0.5167908885183934 1102 7 0.446682051111452 1102 8 0.4891020394208496 1102 9 0.39510221739600127 1102 10-11 0.40101437435178866 1102 12-13 0.4777592536477968 1102 14-15 0.6486357547885504 1102 16-17 0.4095603526037408 1102 18-19 0.38373211025726306 1102 20-21 0.43612273463647 1102 22-23 0.45530941132358294 1102 24-25 0.300216497514306 1102 26-27 0.4237780478591162 1102 28-29 0.35434261343153395 1102 30-31 0.5720894565625514 1102 32-33 0.674536524009028 1102 34-35 0.7868384145973053 1102 36-37 0.8727521628095403 1102 38-39 0.8894889391872951 1102 40-41 0.6162884582503025 1102 42-43 0.397248904938742 1102 44-45 0.4133728646698316 1102 46-47 0.38900844754308395 1102 48-49 0.23379344825569603 1102 50-51 0.31864676104635237 1102 52-53 0.397206561957276 1102 54-55 0.2534326599661796 1102 56-57 0.3347503586465663 1102 58-59 0.310160496711255 1102 60-61 0.33698264481716933 1102 62-63 0.3837650668094881 1102 64-65 0.3928221836185557 1102 66-67 0.38791314566625346 1102 68-69 0.44232948817982276 1102 70-71 0.5194366558055457 1102 72-73 0.3816755347685117 1102 74-75 0.42006705852778836 1102 76-77 0.47377815343889296 1102 78-79 0.4886788692570647 1102 80-81 0.3580679476210271 1102 82-83 0.36463874673571794 1102 84-85 0.5299574988234035 1102 86-87 0.4322677442744123 1102 88-89 0.323221658187407 1102 90-91 0.3298316835620909 1102 92-93 0.39935761130817937 1102 94-95 0.3557074481797997 1102 96-97 0.23014530678914724 1102 98-99 0.25504564894203696 1103 1 0.23221120999884803 1103 2 0.29319975116138863 1103 3 0.18947546266376492 1103 4 0.3159162189132445 1103 5 0.24000558969797936 1103 6 0.3664375764176526 1103 7 0.20790070429256247 1103 8 0.20289563147162681 1103 9 0.20332912698307126 1103 10-11 0.2762416993180423 1103 12-13 0.2950391807297237 1103 14-15 0.38705243287605384 1103 16-17 0.22237407309400226 1103 18-19 0.23486482736722536 1103 20-21 0.2628844027590276 1103 22-23 0.3092246138704269 1103 24-25 0.2227144298466115 1103 26-27 0.342341485203022 1103 28-29 0.26479272259991404 1103 30-31 0.48723800288691876 1103 32-33 0.5654176608083716 1103 34-35 0.5815800392367905 1103 36-37 0.7228261675976313 1103 38-39 0.6690323861140257 1103 40-41 0.5057619846032182 1103 42-43 0.47225443975617765 1103 44-45 0.2702645533032424 1103 46-47 0.2459217363850854 1103 48-49 0.21153446634281892 1103 50-51 0.164646969325986 1103 52-53 0.2771938582401958 1103 54-55 0.1638555092559315 1103 56-57 0.23321581676457015 1103 58-59 0.305500058173962 1103 60-61 0.16586288462252696 1103 62-63 0.18340137140917534 1103 64-65 0.30842422103557965 1103 66-67 0.30243828334799616 1103 68-69 0.40712794544156594 1103 70-71 0.4094612895783989 1103 72-73 0.10488846515939443 1103 74-75 0.21714208157500892 1103 76-77 0.23826030264605436 1103 78-79 0.298847947249989 1103 80-81 0.2199354680472645 1103 82-83 0.3119124109844371 1103 84-85 0.3677161937735427 1103 86-87 0.33256961190982537 1103 88-89 0.24834166514176914 1103 90-91 0.23424256133273502 1103 92-93 0.2519267942131691 1103 94-95 0.3447144949929566 1103 96-97 0.29569365097786005 1103 98-99 0.17805915408539263 1104 1 0.22046219663790723 1104 2 0.21714725945562563 1104 3 0.18152475796741285 1104 4 0.2609077927717607 1104 5 0.2185016162669271 1104 6 0.2763432565526074 1104 7 0.23785035859967252 1104 8 0.29808375436379464 1104 9 0.27987628708813617 1104 10-11 0.2317292425543087 1104 12-13 0.2916412850467438 1104 14-15 0.4272143156698931 1104 16-17 0.32047331364025666 1104 18-19 0.3395358884106159 1104 20-21 0.23906911039856027 1104 22-23 0.15753244792355048 1104 24-25 0.13969073992346637 1104 26-27 0.2645755240166068 1104 28-29 0.19462541195870386 1104 30-31 0.3971086240356243 1104 32-33 0.4334742059634209 1104 34-35 0.5564688048332087 1104 36-37 0.6414695002938515 1104 38-39 0.569865739802836 1104 40-41 0.40184956029073504 1104 42-43 0.34537622964734993 1104 44-45 0.19303356575930763 1104 46-47 0.21239274518227802 1104 48-49 0.11354055664553897 1104 50-51 0.07540289283270596 1104 52-53 0.16210221070184616 1104 54-55 -0.048959425062662376 1104 56-57 0.12330400620351867 1104 58-59 0.15591790411757955 1104 60-61 0.0018441833627633741 1104 62-63 0.15214569291897817 1104 64-65 0.15463757211869478 1104 66-67 0.11182425854662625 1104 68-69 0.20672489953847872 1104 70-71 0.19833938066015477 1104 72-73 0.04630398044987416 1104 74-75 0.013289763977827107 1104 76-77 0.13381313785806626 1104 78-79 0.1116214515279168 1104 80-81 0.03906723985391025 1104 82-83 0.17004981853944656 1104 84-85 0.1719417874472029 1104 86-87 0.05218792484771839 1104 88-89 0.006293808478350371 1104 90-91 -0.1117290375681037 1104 92-93 0.061234019597897316 1104 94-95 0.06818344859114944 1104 96-97 -0.013839014227631452 1104 98-99 -0.05503245131502155 1105 1 0.0578621055163957 1105 2 0.08228031155169191 1105 3 0.025906453339988644 1105 4 -0.08431414699675344 1105 5 -0.05208561011863111 1105 6 0.1421493762728261 1105 7 0.05937502410512252 1105 8 0.11646717861157896 1105 9 -0.007002737364118161 1105 10-11 0.033550561150164526 1105 12-13 0.09389211196499048 1105 14-15 0.14504963961578454 1105 16-17 -0.014653411654883541 1105 18-19 0.028749689716775606 1105 20-21 0.0336504795133834 1105 22-23 0.06696747709714401 1105 24-25 -0.10868535999341589 1105 26-27 0.043309197930739174 1105 28-29 0.046316121501902785 1105 30-31 0.18422074981413772 1105 32-33 0.2986716102609961 1105 34-35 0.3482007778545224 1105 36-37 0.4270942487345195 1105 38-39 0.31077640385439054 1105 40-41 0.20539089132974908 1105 42-43 0.04372092684175044 1105 44-45 0.05984288323407583 1105 46-47 -0.02015785530708314 1105 48-49 -0.13541773437938787 1105 50-51 -0.07801141174158488 1105 52-53 -0.004245725393374755 1105 54-55 -0.06261183214261479 1105 56-57 0.10623394370056616 1105 58-59 -0.03433747589604508 1105 60-61 -0.03769217929686164 1105 62-63 -0.027339334427665563 1105 64-65 0.04181471103544965 1105 66-67 -0.03357346845445619 1105 68-69 0.14769439975795962 1105 70-71 0.14809070984004435 1105 72-73 -0.006968311399095484 1105 74-75 0.10643991618022497 1105 76-77 0.011602813106570409 1105 78-79 -0.0022178900357516795 1105 80-81 -0.09094770563521948 1105 82-83 0.028963163397939695 1105 84-85 -0.005036054543520407 1105 86-87 -0.08373159452201051 1105 88-89 -0.12494979829566688 1105 90-91 -0.13957461316387665 1105 92-93 -0.15948909053600246 1105 94-95 -0.15388584249897974 1105 96-97 -0.2757708717961833 1105 98-99 -0.16498763313081 1106 1 0.15082537158106746 1106 2 0.13735672160777668 1106 3 0.01486577932057287 1106 4 -0.025371712877074515 1106 5 0.04097599094774296 1106 6 0.07504586353550735 1106 7 0.05755641687420621 1106 8 0.15418738821443156 1106 9 0.10133165714809422 1106 10-11 0.08218776317026766 1106 12-13 0.0459439633175549 1106 14-15 0.18315704555823942 1106 16-17 0.011092826863297489 1106 18-19 0.1791088158996743 1106 20-21 0.059855513295559604 1106 22-23 0.06421003759746213 1106 24-25 0.08452234410897574 1106 26-27 0.21879135502288705 1106 28-29 0.11313702554712535 1106 30-31 0.23784536998520167 1106 32-33 0.252358535300246 1106 34-35 0.3120226741315051 1106 36-37 0.37115032380609136 1106 38-39 0.34713790943918355 1106 40-41 0.16156208744571643 1106 42-43 0.04073905958720303 1106 44-45 0.02025559363787721 1106 46-47 0.03563220095364983 1106 48-49 -0.04027933313178522 1106 50-51 -0.09373971293105399 1106 52-53 -0.0029365930050602174 1106 54-55 -0.126918709419094 1106 56-57 -0.15626217552692623 1106 58-59 -0.12009125518677877 1106 60-61 -0.1996678586207139 1106 62-63 -0.06757961002868029 1106 64-65 0.05326421818256222 1106 66-67 -0.008189492885854577 1106 68-69 -0.011453907605876168 1106 70-71 0.036974260292303995 1106 72-73 -0.20108157297838858 1106 74-75 0.04192202913604248 1106 76-77 0.00634125480701897 1106 78-79 -0.024636962681469754 1106 80-81 -0.045522688008386325 1106 82-83 -0.03368942363630012 1106 84-85 0.01477309472739563 1106 86-87 -0.08601557205767563 1106 88-89 -0.20365966559554138 1106 90-91 -0.2037081100667848 1106 92-93 -0.2068985308378366 1106 94-95 -0.027209159429766316 1106 96-97 -0.2524704848659347 1106 98-99 -0.23713916518886435 1107 1 0.04282094784596424 1107 2 0.07708354544881857 1107 3 0.055279202819811246 1107 4 -0.006093095901661627 1107 5 0.01968043449221568 1107 6 0.114515820145904 1107 7 0.02979845562116168 1107 8 0.06909787797376055 1107 9 -0.0028529168248780934 1107 10-11 0.012991141537199269 1107 12-13 0.001723191636330057 1107 14-15 0.04608907709613419 1107 16-17 -0.043210844781043534 1107 18-19 0.20741070319829902 1107 20-21 0.06691069687992268 1107 22-23 -0.028613336429849312 1107 24-25 0.06788526272679007 1107 26-27 0.060091736122551254 1107 28-29 0.07513546805412119 1107 30-31 0.2550887780039446 1107 32-33 0.20391729991663254 1107 34-35 0.2186302904160513 1107 36-37 0.3121365611223439 1107 38-39 0.3464595243385773 1107 40-41 0.16196036739999187 1107 42-43 0.07136699195891083 1107 44-45 0.07154576743881336 1107 46-47 -0.08177663033425375 1107 48-49 -0.17606816996116237 1107 50-51 -0.1604141541855384 1107 52-53 -0.03159311203718573 1107 54-55 -0.061888842983186976 1107 56-57 0.018656607126068536 1107 58-59 -0.02989163016980001 1107 60-61 -0.09175376282620107 1107 62-63 -0.02164255382135849 1107 64-65 0.043768972968095454 1107 66-67 0.1166538886059314 1107 68-69 0.1389346286275739 1107 70-71 0.05808140774076875 1107 72-73 -0.1821437716125054 1107 74-75 0.023429911784731416 1107 76-77 -0.058093928094855585 1107 78-79 -0.05688594221987131 1107 80-81 -0.04587313466355525 1107 82-83 -0.020851481244008596 1107 84-85 0.0047919511880074595 1107 86-87 -0.2509309980632395 1107 88-89 -0.2491847330343191 1107 90-91 -0.2009338860285972 1107 92-93 -0.2679187166510282 1107 94-95 -0.1888706870348642 1107 96-97 -0.2595348702571343 1107 98-99 -0.24529357569268484 1108 1 0.04288786703974168 1108 2 0.04588287426682314 1108 3 -0.044010994751577215 1108 4 0.01822568947923031 1108 5 -0.038090572651455545 1108 6 0.07643082814502833 1108 7 0.07328126891528086 1108 8 0.0326174225476521 1108 9 0.05242102337654586 1108 10-11 -0.04466797741521589 1108 12-13 -0.012837689168812005 1108 14-15 0.02833257705265879 1108 16-17 -0.0626301203678068 1108 18-19 0.17426481192818954 1108 20-21 0.1718230351886092 1108 22-23 0.007662735165418155 1108 24-25 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-1.8525105776845407E-4 2215 94-95 0.15582452066198726 2215 96-97 0.21685814867512931 2215 98-99 0.2939976550701573 2216 1 -0.389365392913426 2216 2 -0.4144774875657973 2216 3 -0.27575452979538895 2216 4 -0.4049153082650747 2216 5 -0.40778722986860316 2216 6 -0.3633028585252376 2216 7 -0.5722559702880545 2216 8 -0.4884336556763529 2216 9 -0.4692333870243175 2216 10-11 -0.5456705050402206 2216 12-13 -0.5397379632740353 2216 14-15 -0.6438667426931417 2216 16-17 -0.6696991849798621 2216 18-19 -0.614436294085472 2216 20-21 -0.3646823201831779 2216 22-23 -0.36535965197352027 2216 24-25 -0.4099789992242435 2216 26-27 -0.5248473703463503 2216 28-29 -0.5264955586796063 2216 30-31 -0.37666001761268575 2216 32-33 -0.2751269481420451 2216 34-35 -0.33390954472661605 2216 36-37 -0.24711029868700507 2216 38-39 -8.585912517235744 2216 40-41 -2.0859075109124134 2216 42-43 -0.7959784423519238 2216 44-45 -0.756670266348145 2216 46-47 -0.5545502214107358 2216 48-49 -0.4191010422907624 2216 50-51 -0.4739479007984926 2216 52-53 -0.459372004146104 2216 54-55 -0.45326172145862387 2216 56-57 -0.40284773333096524 2216 58-59 -0.4999807069469995 2216 60-61 -0.49095288534806514 2216 62-63 -0.5577900536081017 2216 64-65 -0.6560747297380196 2216 66-67 -0.5599188498260759 2216 68-69 -0.5124484813839203 2216 70-71 -0.32720559236232205 2216 72-73 -0.3614145774649522 2216 74-75 -0.3207812998577104 2216 76-77 -0.5065959541464906 2216 78-79 -0.4450813713103692 2216 80-81 -0.4268231359974557 2216 82-83 -0.48995843430805053 2216 84-85 -0.4039273332071964 2216 86-87 -0.35007091007828706 2216 88-89 -0.26832974352605277 2216 90-91 -0.2944884629488982 2216 92-93 -0.2551285143554587 2216 94-95 -0.10796191320862647 2216 96-97 -0.01638261353895132 2216 98-99 -0.05077656249098794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 238.0 8 1324.0 9 2461.0 10 3904.0 11 8361.0 12 19725.0 13 39593.0 14 56558.0 15 58889.0 16 58920.0 17 60663.0 18 60406.0 19 62166.0 20 63952.0 21 67586.0 22 72756.0 23 78579.0 24 86951.0 25 92391.0 26 97762.0 27 105741.0 28 125258.0 29 153357.0 30 191695.0 31 245211.0 32 323987.0 33 427828.0 34 578138.0 35 813780.0 36 1186565.0 37 1296185.0 38 392752.0 39 260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.96927220493352 20.042089903295945 12.544719790609902 23.443918101160634 2 15.241209313419557 21.801312447915105 38.39712780380447 24.560350434860865 3 17.531470723468843 29.562695160045504 28.733119543238058 24.172714573247596 4 11.980682308880832 18.789650425822984 37.32877198419712 31.900895281099068 5 12.90803646856691 40.05583577994123 32.48481659257455 14.551311158917299 6 28.83198925832579 39.089857920989694 18.19728110151327 13.880871719171243 7 25.903393905906675 34.368316403995614 22.06927058884127 17.659019101256444 8 26.82235909454336 34.02834439380832 19.542843780985038 19.60645273066329 9 27.137462642362888 16.74822035416152 19.572270419541045 36.54204658393454 10-11 24.717849522281576 27.785047048979926 25.30207309339178 22.195030335346715 12-13 25.553017811335476 26.331485910495626 25.27800054273274 22.837495735436153 14-15 24.468874835430693 26.216934867503877 23.187484113766576 26.126706183298854 16-17 23.12600509516486 28.172325440851747 24.667102238389916 24.034567225593474 18-19 23.804767788556653 27.795369701595448 26.323452460248433 22.07641004959947 20-21 26.194698112832064 26.579432023440635 24.585027328289492 22.64084253543781 22-23 25.218354972628532 26.84332510333349 23.971695102430647 23.966624821607326 24-25 23.780334470731564 27.789701635449248 24.26042105122989 24.1695428425893 26-27 24.368387138360685 27.131361646885928 24.342727894436976 24.157523320316407 28-29 23.538225213396778 27.075448057323825 25.35507475152029 24.031251977759105 30-31 24.30254593898488 27.415917329911306 24.807596924418903 23.47393980668491 32-33 23.45408555369099 28.075959439756364 24.061521969058884 24.408433037493758 34-35 23.647237963904885 28.157093473402767 25.24275879345807 22.95290976923428 36-37 24.06462544594559 27.646698639956035 24.635519207203288 23.653156706895086 38-39 24.30113051361852 27.194400259099222 24.585984454046788 23.91848477323547 40-41 23.562546351062878 27.842143279000766 25.197893795996208 23.397416573940152 42-43 24.37851385913637 27.45667330243697 24.824224275915373 23.340588562511293 44-45 23.672645767798016 27.99720659048565 24.740199379786997 23.589948261929333 46-47 22.94184067745296 27.918543921863552 25.623365545092426 23.516249855591063 48-49 23.75037606439393 27.873914827414247 25.285969900800943 23.08973920739088 50-51 22.73250699655753 28.308996192728408 24.752321022128445 24.206175788585618 52-53 22.589560361692612 28.137880364349943 24.216743980709765 25.05581529324768 54-55 23.159379122802292 27.87105805959194 24.367078512788513 24.60248430481725 56-57 23.86925847664255 26.975517608034576 24.11040880467943 25.04481511064344 58-59 23.213632070560724 27.084581545047893 25.462370299081343 24.239416085310044 60-61 23.551730329147308 27.598947077458053 24.744308948978265 24.105013644416367 62-63 22.78194338447734 27.614888544870574 24.519294401025117 25.083873669626968 64-65 22.55604127194284 27.6776469396941 24.74620611505128 25.020105673311782 66-67 24.087947933975542 27.254334902748617 24.373489278353095 24.28422788492275 68-69 23.98860936238359 26.925405824938125 24.434133691393647 24.65185112128464 70-71 23.088896688198172 27.422920179922606 24.724490862772615 24.763692269106606 72-73 24.189231150591866 27.272454564956476 24.632299465804056 23.906014818647602 74-75 24.65385874390149 27.072153984222346 24.71362014023442 23.560367131641744 76-77 23.866436560131675 27.39493755867775 25.043759555254834 23.69486632593574 78-79 24.04194451116623 27.367628028572415 24.90413463929668 23.686292820964674 80-81 23.63205639378959 26.985039678405325 25.62494659013887 23.757957337666213 82-83 24.108657687779658 26.9130289121742 24.938064468265324 24.040248931780813 84-85 24.337937869563163 27.162622643864502 24.261627269764773 24.23781221680756 86-87 24.04079547622866 26.457701438583637 24.925423737742072 24.576079347445635 88-89 24.47244226759833 25.641979214345334 25.13938510470674 24.746193413349594 90-91 24.69487796508731 26.02357656420451 24.99077028433963 24.290775186368556 92-93 24.306454662197993 26.240835453143895 25.020273797970834 24.43243608668728 94-95 23.29473288045673 26.615070302010086 25.595717494904846 24.494479322628337 96-97 24.261511794088204 26.524101036013725 24.64663757035586 24.567749599542214 98-99 23.943583445554033 26.936135357000836 24.458119455501887 24.66216174194325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1932.0 1 1525.5 2 1081.5 3 955.5 4 806.5 5 703.0 6 610.5 7 549.5 8 572.0 9 581.0 10 527.0 11 509.0 12 553.5 13 607.5 14 640.0 15 813.0 16 1010.5 17 1185.0 18 1607.0 19 2171.5 20 2839.5 21 3895.0 22 5475.5 23 7636.5 24 10478.5 25 14785.5 26 20825.5 27 27035.0 28 35905.5 29 50245.0 30 62436.5 31 76410.5 32 94195.5 33 109483.0 34 122664.0 35 134922.5 36 140808.5 37 152280.0 38 166084.0 39 154680.5 40 142625.0 41 147208.0 42 154089.0 43 170059.0 44 196821.5 45 231613.0 46 267942.5 47 300259.0 48 322627.0 49 348087.0 50 382428.0 51 345736.5 52 286066.5 53 272027.5 54 248336.5 55 235014.5 56 216840.0 57 189572.5 58 181784.0 59 163384.5 60 130135.0 61 94684.5 62 74182.0 63 60880.0 64 45799.5 65 37702.5 66 30863.5 67 23532.5 68 18142.0 69 15058.5 70 13568.0 71 12841.5 72 13221.0 73 11907.5 74 8445.0 75 6373.5 76 5471.0 77 4210.5 78 2713.5 79 2042.5 80 1852.5 81 1536.0 82 1382.5 83 1151.0 84 780.0 85 616.0 86 413.5 87 227.0 88 143.5 89 128.0 90 106.5 91 58.5 92 41.5 93 46.0 94 40.0 95 27.0 96 14.5 97 8.5 98 7.5 99 5.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.4632838665344638E-5 2 0.0021802929611363514 3 0.006584777399405088 4 1.4632838665344638E-5 5 0.004872735275559765 6 0.0 7 7.023762559365427E-4 8 0.0 9 0.0 10-11 2.9265677330689276E-5 12-13 1.3901196732077407E-4 14-15 0.002882669217072894 16-17 2.0485974131482495E-4 18-19 0.0 20-21 0.0 22-23 4.389851599603392E-5 24-25 0.0010608808032374862 26-27 0.0021876093804690236 28-29 0.0019973824778195433 30-31 0.002611961701764018 32-33 8.779703199206784E-4 34-35 0.0 36-37 1.536448059861187E-4 38-39 0.06040435801054267 40-41 0.0033362872156985773 42-43 3.292388699702544E-4 44-45 2.194925799801696E-5 46-47 7.682240299305935E-4 48-49 1.170627093227571E-4 50-51 6.950598366038704E-4 52-53 2.8534035397422046E-4 54-55 0.0 56-57 4.7556725662370073E-4 58-59 7.316419332672319E-6 60-61 1.902269026494803E-4 62-63 8.048061265939552E-5 64-65 7.609076105979212E-4 66-67 0.0012072091898909327 68-69 0.0012584241252196389 70-71 8.048061265939552E-5 72-73 0.001785206317172046 74-75 0.0014779167051998084 76-77 1.170627093227571E-4 78-79 1.536448059861187E-4 80-81 0.0 82-83 3.43871708635599E-4 84-85 4.536179986256838E-4 86-87 0.0 88-89 0.0 90-91 5.706807079484409E-4 92-93 0.0 94-95 0.0 96-97 0.0029631498297322894 98-99 0.004558129244254854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6833944.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.84491320538197 #Duplication Level Percentage of deduplicated Percentage of total 1 75.8531872052227 21.879786012862393 2 12.807068217401202 7.388375422926874 3 4.506753830461813 3.8999076923308125 4 2.0790139786144604 2.398759110636399 5 1.0866059549744442 1.567152722984452 6 0.6505290455715524 1.1258672314254843 7 0.4343196663514107 0.8769539155507831 8 0.30173829535662555 0.696289195224142 9 0.2289923971559548 0.5944739238590285 >10 1.5118867445335649 8.709120514526093 >50 0.2208001462270934 4.486792141495897 >100 0.2391171893996207 14.918079602452003 >500 0.04592152857663686 9.385846552949095 >1k 0.03230556123953257 17.079173226637206 >5k 0.0012425215861358605 2.2966247148676313 >10k+ 5.177173275566085E-4 2.6967980192717405 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 41984 0.6143450985258293 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 38014 0.5562527290244111 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 16260 0.23792995669850384 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 15119 0.2212338877813456 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 12545 0.1835689610567485 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 12297 0.179940017067743 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 12193 0.1784182018465472 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 10943 0.16012715351486637 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 10629 0.15553244217394815 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 10603 0.15515198836864919 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 8971 0.13127119566680676 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 8904 0.13029079547622865 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 8001 0.11707734216142246 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7912 0.11577501952020677 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 7706 0.1127606547551458 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 7686 0.11246799798183889 No Hit GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA 7634 0.11170709037124098 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 7270 0.10638073709705552 No Hit GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA 6946 0.10163969736948386 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4632838665344638E-5 2 0.0 0.0 0.0 0.0 1.4632838665344638E-5 3 1.4632838665344638E-5 0.0 0.0 0.0 1.4632838665344638E-5 4 1.4632838665344638E-5 0.0 0.0 0.0 1.4632838665344638E-5 5 1.4632838665344638E-5 0.0 0.0 1.4632838665344638E-5 1.4632838665344638E-5 6 1.4632838665344638E-5 0.0 0.0 1.4632838665344638E-5 1.4632838665344638E-5 7 1.4632838665344638E-5 0.0 0.0 2.9265677330689276E-5 1.4632838665344638E-5 8 2.9265677330689276E-5 0.0 0.0 2.9265677330689276E-5 1.4632838665344638E-5 9 2.9265677330689276E-5 0.0 0.0 2.9265677330689276E-5 1.4632838665344638E-5 10-11 2.9265677330689276E-5 0.0 0.0 5.853135466137855E-5 1.4632838665344638E-5 12-13 5.853135466137855E-5 0.0 0.0 5.853135466137855E-5 2.9265677330689276E-5 14-15 8.779703199206784E-5 0.0 0.0 5.853135466137855E-5 2.9265677330689276E-5 16-17 9.511345132474015E-5 0.0 0.0 8.779703199206782E-5 2.9265677330689276E-5 18-19 1.170627093227571E-4 0.0 0.0 1.2437912865542945E-4 2.9265677330689276E-5 20-21 1.3169554798810176E-4 0.0 0.0 4.316687406276668E-4 3.65820966633616E-5 22-23 1.536448059861187E-4 0.0 0.0 0.0024217347991145376 4.389851599603392E-5 24-25 2.1217616064749727E-4 0.0 0.0 0.007565177589983178 4.389851599603392E-5 26-27 2.7070751530887583E-4 0.0 0.0 0.012035509802245966 4.389851599603392E-5 28-29 3.146060313049097E-4 0.0 0.0 0.028804742912730923 4.389851599603392E-5 30-31 3.804538052989606E-4 0.0 0.0 0.08035623353074009 4.389851599603392E-5 32-33 4.0971948262964985E-4 0.0 0.0 0.16536570975705978 4.389851599603392E-5 34-35 4.3166874062766685E-4 0.0 0.0 0.2725585694000419 5.853135466137855E-5 36-37 4.536179986256838E-4 0.0 0.0 0.44149469179144574 5.853135466137855E-5 38-39 4.682508372910284E-4 0.0 0.0 0.7110169471684287 5.853135466137855E-5 40-41 4.8288367595637305E-4 0.0 0.0 1.0546106318693862 5.853135466137855E-5 42-43 5.414150306177516E-4 0.0 0.0 1.3456943750197543 5.853135466137855E-5 44-45 5.853135466137856E-4 0.0 0.0 1.6715018443229854 6.584777399405088E-5 46-47 5.853135466137856E-4 0.0 0.0 2.010427653489698 7.31641933267232E-5 48-49 5.999463852791302E-4 0.0 0.0 2.378706937019092 7.31641933267232E-5 50-51 6.145792239444748E-4 0.0 0.0 2.811326812160006 7.31641933267232E-5 52-53 6.145792239444748E-4 0.0 0.0 3.2341792674918026 7.31641933267232E-5 54-55 6.145792239444748E-4 0.0 0.0 3.611677239380364 7.31641933267232E-5 56-57 6.145792239444748E-4 0.0 0.0 4.041019358660241 7.31641933267232E-5 58-59 6.145792239444748E-4 0.0 0.0 4.5093579929832615 7.31641933267232E-5 60-61 6.292120626098194E-4 0.0 0.0 4.97804781543425 7.31641933267232E-5 62-63 6.292120626098194E-4 0.0 0.0 5.44255264602695 8.779703199206784E-5 64-65 6.438449012751641E-4 0.0 0.0 5.929211594358982 8.779703199206784E-5 66-67 6.584777399405087E-4 0.0 0.0 6.4438412137998204 8.779703199206784E-5 68-69 6.584777399405087E-4 0.0 0.0 6.992243424880274 8.779703199206784E-5 70-71 6.731105786058533E-4 0.0 0.0 7.64211705568556 8.779703199206784E-5 72-73 6.804269979385257E-4 0.0 0.0 8.244243733925828 9.511345132474015E-5 74-75 6.950598366038704E-4 0.0 0.0 8.822723744882897 1.0242987065741246E-4 76-77 7.023762559365427E-4 0.0 0.0 9.43516657438223 1.0242987065741246E-4 78-79 7.023762559365427E-4 0.0 0.0 10.047777681526217 1.0242987065741246E-4 80-81 7.023762559365427E-4 7.316419332672319E-6 0.0 10.719015256782907 1.0242987065741246E-4 82-83 7.023762559365427E-4 1.4632838665344638E-5 0.0 11.452888697946603 1.3169554798810176E-4 84-85 7.243255139345597E-4 1.4632838665344638E-5 0.0 12.143946453175502 1.3169554798810176E-4 86-87 7.316419332672319E-4 2.1949257998016955E-5 0.0 12.830877747900772 1.3169554798810176E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATA 1725 0.0 42.320576 1 TACATGA 2430 0.0 32.52997 2 GTACAAA 3865 0.0 29.114336 1 GTACAAG 3750 0.0 28.891205 1 ACGTTAA 855 0.0 26.10206 8 GTACTAG 3850 0.0 25.483715 1 CTATTGA 4100 0.0 24.722443 9 TAGGCAT 5080 0.0 24.346329 5 TACAAGA 2345 0.0 24.191233 2 GTGATCG 2890 0.0 24.131989 8 AGGGCTA 4155 0.0 23.9474 5 TATTCGC 615 0.0 23.437178 9 CGTTAAC 1080 0.0 23.249361 1 GTATAAG 3935 0.0 22.9244 1 TAGTACT 4525 0.0 22.811296 4 GGGCTAT 4545 0.0 22.608118 6 ATAAGGT 3255 0.0 22.570807 3 TGATCGC 3185 0.0 22.189775 9 TACATAA 1850 0.0 21.866966 2 ATTTAGG 4710 0.0 21.81787 1 >>END_MODULE