FastQCFastQC Report
Fri 27 May 2016
ERR765383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6833944
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT430190.629490086544461No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA395760.5791092230196794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313450.45866632796522766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211090.30888459138676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161140.2357935622533635No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG159170.2329108930362906No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG137730.2015380869377917No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG127710.18687598259511637No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT127620.18674428704712828No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC122050.17859379591053132No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC114000.1668143607849289No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA113730.16641927414096458No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC100200.14662104342675328No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC89690.13124192998947606No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC88420.12938355947897728No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA87850.12854948767505264No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC79390.11617010616417109No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT73960.10822447476888895No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT72270.1057515250344457No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA70730.10349806787998263No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC70650.10338100517065987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4500.051.6698652
TCACGCA6450.036.769724
CTATTGA38300.025.1341449
TAACGCA5850.024.6425534
AGGGCTA40000.024.2977545
CCGAGTA7300.023.5696078
GTATCAA959650.023.485681
AGTACTT527800.022.91814212-13
GGGCTAT44800.022.8355676
ATTTAGG44650.022.4041731
GGCTATT45600.021.8230827
CGAGTAC8000.021.5082119
GTACTAG38700.021.2801251
ATCACGC12400.020.6262783
TAGGCAT48600.020.5722625
TCAACGA9300.020.501273
CTGTGCG43600.020.1589499
TATTCGC6000.020.1518389
GAGTACT511400.019.97463812-13
TACTTTT614700.019.9012514-15
Produced by FastQC (version 0.11.5)