##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765380_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8165547 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.331047264806635 34.0 31.0 34.0 31.0 34.0 2 32.360847227993425 34.0 31.0 34.0 31.0 34.0 3 32.38923895729214 34.0 31.0 34.0 31.0 34.0 4 35.84080821529776 37.0 35.0 37.0 35.0 37.0 5 35.85753948878134 37.0 37.0 37.0 35.0 37.0 6 35.95429687686569 37.0 37.0 37.0 35.0 37.0 7 35.908610531541854 37.0 37.0 37.0 35.0 37.0 8 35.93891199205638 37.0 37.0 37.0 35.0 37.0 9 37.653060352233595 39.0 38.0 39.0 35.0 39.0 10-11 37.58547626999147 39.0 38.0 39.0 35.0 39.0 12-13 37.55862020021439 39.0 38.0 39.0 35.0 39.0 14-15 38.94634235771345 41.0 39.0 41.0 36.0 41.0 16-17 38.905744648827564 41.0 39.0 41.0 36.0 41.0 18-19 38.910438700554906 41.0 39.0 41.0 35.5 41.0 20-21 38.88648696774386 41.0 39.0 41.0 35.0 41.0 22-23 38.796871538428476 41.0 39.0 41.0 35.0 41.0 24-25 38.717359290198196 41.0 39.0 41.0 35.0 41.0 26-27 38.58699956047035 40.5 38.0 41.0 35.0 41.0 28-29 38.44045579555173 40.0 38.0 41.0 34.0 41.0 30-31 38.201750537961516 40.0 38.0 41.0 34.0 41.0 32-33 37.94206095439779 40.0 38.0 41.0 34.0 41.0 34-35 37.78489205928274 40.0 37.5 41.0 33.0 41.0 36-37 37.62064782677756 40.0 37.0 41.0 33.0 41.0 38-39 37.41052461029249 40.0 37.0 41.0 33.0 41.0 40-41 37.150457525993055 40.0 37.0 41.0 32.0 41.0 42-43 36.85876316675417 40.0 36.0 41.0 31.0 41.0 44-45 36.45389714859274 40.0 36.0 41.0 30.5 41.0 46-47 35.980390535992264 39.5 35.0 41.0 29.5 41.0 48-49 35.55194899986492 39.0 35.0 41.0 27.5 41.0 50-51 34.262978769211664 38.0 34.0 40.0 24.5 40.5 52-53 34.17626026768323 38.0 34.0 39.5 24.0 40.0 54-55 34.973652163168 39.0 35.0 40.5 26.0 41.0 56-57 34.968443387809785 39.0 35.0 41.0 26.0 41.0 58-59 34.74882252223887 38.5 35.0 41.0 26.0 41.0 60-61 34.40809237886941 38.0 35.0 40.5 25.0 41.0 62-63 34.04205309209536 37.0 35.0 40.0 23.0 41.0 64-65 33.74027294191069 36.5 34.0 40.0 22.5 41.0 66-67 33.4242865787191 36.0 34.0 39.0 22.0 41.0 68-69 33.17334484756502 35.5 34.0 39.0 21.5 41.0 70-71 32.83306501083149 35.0 34.0 38.5 20.5 41.0 72-73 32.464500296183466 35.0 34.0 37.0 19.5 39.5 74-75 32.08962957411181 35.0 34.0 37.0 17.5 39.0 76-77 31.71862534132741 35.0 34.0 36.0 12.0 39.0 78-79 31.37963323216436 35.0 33.0 36.0 7.0 37.5 80-81 31.05550375253489 35.0 33.0 35.5 2.0 37.0 82-83 30.766865404118057 35.0 33.0 35.0 2.0 37.0 84-85 30.48364726821118 35.0 33.0 35.0 2.0 36.0 86-87 30.215652729694654 35.0 33.0 35.0 2.0 36.0 88-89 29.95803202161472 35.0 32.0 35.0 2.0 36.0 90-91 29.691822176762926 35.0 32.0 35.0 2.0 35.5 92-93 29.384053205498667 35.0 32.0 35.0 2.0 35.0 94-95 29.033696150423236 35.0 31.0 35.0 2.0 35.0 96-97 28.70242416092884 35.0 31.0 35.0 2.0 35.0 98-99 28.37247020928298 35.0 30.5 35.0 2.0 35.0 100 27.81446019476711 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40224981487597233 1101 2 0.5377730023073113 1101 3 0.5102072396028134 1101 4 0.4643953790223492 1101 5 0.48795627867488633 1101 6 0.44036617576634995 1101 7 0.43274143848739755 1101 8 0.39105718955237734 1101 9 0.46227568244007955 1101 10-11 0.4161869494243291 1101 12-13 0.4334905726813716 1101 14-15 0.6086071952880161 1101 16-17 0.6263760578387476 1101 18-19 0.5704806881370175 1101 20-21 0.6091669837619165 1101 22-23 0.6615613385376875 1101 24-25 0.5570459186010552 1101 26-27 0.664954044084773 1101 28-29 0.691447024274602 1101 30-31 0.6672397374475096 1101 32-33 0.6829964518725404 1101 34-35 0.753577253651514 1101 36-37 0.7605386384678425 1101 38-39 0.800229250005998 1101 40-41 0.80350246485947 1101 42-43 0.723682931447982 1101 44-45 0.824382842250813 1101 46-47 0.8631215916052852 1101 48-49 0.8632788110051095 1101 50-51 0.8362185730467928 1101 52-53 0.8445071099249262 1101 54-55 0.9534706872212766 1101 56-57 1.0619499933517034 1101 58-59 1.0088930024432017 1101 60-61 1.0869491389174186 1101 62-63 1.0552951240830382 1101 64-65 1.0215986529033145 1101 66-67 1.018376777656897 1101 68-69 0.9961049443028642 1101 70-71 0.915726514223536 1101 72-73 1.008269774332419 1101 74-75 0.9505021810928369 1101 76-77 0.9183442936893833 1101 78-79 0.9261022034302293 1101 80-81 0.9196962859447382 1101 82-83 0.8256592314768803 1101 84-85 0.8578277789836797 1101 86-87 0.8345528101684216 1101 88-89 0.8447952084712007 1101 90-91 0.8252146799405118 1101 92-93 0.8553611559323784 1101 94-95 0.7738159169275747 1101 96-97 0.7431217534041572 1101 98-99 0.8171156690238917 1101 100 0.7745330534153538 1102 1 0.46612788280877737 1102 2 0.5632789421028832 1102 3 0.5789222966138681 1102 4 0.45939210789210705 1102 5 0.4530379160711817 1102 6 0.42721720663306684 1102 7 0.4863899040470443 1102 8 0.44024165495920187 1102 9 0.40012753637159193 1102 10-11 0.4995606593394726 1102 12-13 0.5162254449944115 1102 14-15 0.7028193883596643 1102 16-17 0.728128686103382 1102 18-19 0.6575443207616445 1102 20-21 0.6468780117014461 1102 22-23 0.6911525008033976 1102 24-25 0.6915381659596704 1102 26-27 0.711005031072709 1102 28-29 0.6579514176036838 1102 30-31 0.6608633489779052 1102 32-33 0.7168044825961744 1102 34-35 0.7094785250459097 1102 36-37 0.7073575668164409 1102 38-39 0.7214572885463895 1102 40-41 0.754214284218591 1102 42-43 0.7391348129339832 1102 44-45 0.755426900578712 1102 46-47 0.7483842260744993 1102 48-49 0.6962314370700113 1102 50-51 0.687077875468745 1102 52-53 0.7141558259448075 1102 54-55 0.8386368424029982 1102 56-57 0.7612943876494285 1102 58-59 0.8618662866531395 1102 60-61 0.8187041096991479 1102 62-63 0.8265303358584575 1102 64-65 0.82597744254943 1102 66-67 0.7979971731467188 1102 68-69 0.8185252883499317 1102 70-71 0.746701942010283 1102 72-73 0.7464507721771696 1102 74-75 0.7056843007022664 1102 76-77 0.6989836792938426 1102 78-79 0.63605331351609 1102 80-81 0.6721267439155127 1102 82-83 0.6271113219370186 1102 84-85 0.6839489720195395 1102 86-87 0.706837943318039 1102 88-89 0.6105458380709123 1102 90-91 0.6314269137264183 1102 92-93 0.6646030805436567 1102 94-95 0.6479036208449926 1102 96-97 0.6798048090073898 1102 98-99 0.7574579428033559 1102 100 0.7921091193739969 1103 1 0.504290533469586 1103 2 0.5526645099653109 1103 3 0.5490521710605378 1103 4 0.4271498007932095 1103 5 0.493240077792251 1103 6 0.48568605263765363 1103 7 0.4748082068514208 1103 8 0.464501393880731 1103 9 0.5455941461989724 1103 10-11 0.5432665946961421 1103 12-13 0.5197253407059534 1103 14-15 0.6797287050445178 1103 16-17 0.6390747222971527 1103 18-19 0.6411788579965005 1103 20-21 0.6442466324817531 1103 22-23 0.6740748157357075 1103 24-25 0.6602315465371973 1103 26-27 0.5967075821096515 1103 28-29 0.6307083086054774 1103 30-31 0.6603495932887782 1103 32-33 0.648605855879886 1103 34-35 0.6236333356522579 1103 36-37 0.5642082243600157 1103 38-39 0.5885415106860634 1103 40-41 0.5751521102931463 1103 42-43 0.5427256459646941 1103 44-45 0.534295690741196 1103 46-47 0.5538193582383073 1103 48-49 0.5857325532196498 1103 50-51 0.563814458336239 1103 52-53 0.5590888611211753 1103 54-55 0.5938304906957868 1103 56-57 0.6177463115014064 1103 58-59 0.6086513491112626 1103 60-61 0.581632416024128 1103 62-63 0.4883246679400486 1103 64-65 0.4929709628153276 1103 66-67 0.49112956353511805 1103 68-69 0.5101900229794367 1103 70-71 0.4927189677588686 1103 72-73 0.4443547403737611 1103 74-75 0.431234612750508 1103 76-77 0.4113020297135854 1103 78-79 0.442413815308381 1103 80-81 0.42443687867614344 1103 82-83 0.3352871492297922 1103 84-85 0.396722563024305 1103 86-87 0.4400877350873671 1103 88-89 0.39912121064939043 1103 90-91 0.445412106704385 1103 92-93 0.4431283281837288 1103 94-95 0.4235513103362898 1103 96-97 0.5080991262697729 1103 98-99 0.539692701800071 1103 100 0.668663024318878 1104 1 0.38661200455313605 1104 2 0.4834644254557574 1104 3 0.4988920117635942 1104 4 0.457014639708909 1104 5 0.432069072510572 1104 6 0.4239360494184723 1104 7 0.39350899695848085 1104 8 0.3990297534637506 1104 9 0.4512749074300615 1104 10-11 0.5059097058650011 1104 12-13 0.5116954919087675 1104 14-15 0.6227044427135553 1104 16-17 0.6195317480509317 1104 18-19 0.6182096419396359 1104 20-21 0.6050793914562362 1104 22-23 0.6062932750891576 1104 24-25 0.6251450822815698 1104 26-27 0.5943607317140547 1104 28-29 0.5646969941589788 1104 30-31 0.633061023135987 1104 32-33 0.685196307870271 1104 34-35 0.6688033690654436 1104 36-37 0.6889335477297891 1104 38-39 0.7415591068558953 1104 40-41 0.7263221222884582 1104 42-43 0.6928376844176682 1104 44-45 0.6904344541035741 1104 46-47 0.7245805997274672 1104 48-49 0.7389075750895131 1104 50-51 0.691522578168275 1104 52-53 0.6893477888947714 1104 54-55 0.7839845537030925 1104 56-57 0.7733238962495221 1104 58-59 0.7459505570965348 1104 60-61 0.7916558807344316 1104 62-63 0.785396740685897 1104 64-65 0.7806938306303266 1104 66-67 0.6981709363184407 1104 68-69 0.6797606865463166 1104 70-71 0.6981617391379444 1104 72-73 0.6761305477939601 1104 74-75 0.6510091926751329 1104 76-77 0.6280073937937978 1104 78-79 0.6172574485790783 1104 80-81 0.672227640280525 1104 82-83 0.6710634315718096 1104 84-85 0.6323504333823529 1104 86-87 0.5923976743373451 1104 88-89 0.6044187176913844 1104 90-91 0.5435278151575353 1104 92-93 0.5491129587356163 1104 94-95 0.5387566169047382 1104 96-97 0.4979053461153917 1104 98-99 0.48795879493599514 1104 100 0.5631361249231794 1105 1 0.34871265520480677 1105 2 0.3750904704202469 1105 3 0.4009698662365082 1105 4 0.38726901741067365 1105 5 0.38003396547327384 1105 6 0.3589565187350843 1105 7 0.35386376009321197 1105 8 0.38283645885185535 1105 9 0.39641984687343523 1105 10-11 0.47176092898286726 1105 12-13 0.43979041564183063 1105 14-15 0.5674338883563053 1105 16-17 0.5773890932036139 1105 18-19 0.5658231085024283 1105 20-21 0.5166134199433046 1105 22-23 0.519425637525508 1105 24-25 0.5453282776628896 1105 26-27 0.5271307258326416 1105 28-29 0.5773057007511824 1105 30-31 0.6172314744604677 1105 32-33 0.6278272773757578 1105 34-35 0.5559664101306225 1105 36-37 0.5545590172789474 1105 38-39 0.6118821841815745 1105 40-41 0.5814558697428822 1105 42-43 0.5869090895745046 1105 44-45 0.6422329311260597 1105 46-47 0.6002323802036287 1105 48-49 0.6726324010804205 1105 50-51 0.6939305245482359 1105 52-53 0.632620565572438 1105 54-55 0.660326886812939 1105 56-57 0.6707776727579073 1105 58-59 0.6814517401497611 1105 60-61 0.6124945955518584 1105 62-63 0.6511396332382944 1105 64-65 0.646807190627193 1105 66-67 0.6546104016125938 1105 68-69 0.6439135860179945 1105 70-71 0.7047573497142281 1105 72-73 0.6622093941057869 1105 74-75 0.6755763629041454 1105 76-77 0.6635578375595763 1105 78-79 0.6702525884250896 1105 80-81 0.7113381782706156 1105 82-83 0.6437259830613229 1105 84-85 0.5887202526177582 1105 86-87 0.6218453374113544 1105 88-89 0.6844055164170264 1105 90-91 0.6782024793470214 1105 92-93 0.6906864568093063 1105 94-95 0.6545044924771872 1105 96-97 0.7101410419374368 1105 98-99 0.7219563223895307 1105 100 0.7973424065347281 1106 1 0.2643510931779929 1106 2 0.28488515814569837 1106 3 0.297053033207078 1106 4 0.3178311530370195 1106 5 0.29425684637644167 1106 6 0.30883201966624085 1106 7 0.29029635958825395 1106 8 0.32947118970541567 1106 9 0.34478098741475094 1106 10-11 0.3112318241397105 1106 12-13 0.37660649596335105 1106 14-15 0.5099394049485682 1106 16-17 0.44624668405556633 1106 18-19 0.44008983682170566 1106 20-21 0.44276293503811814 1106 22-23 0.46804696661624945 1106 24-25 0.46751901245749394 1106 26-27 0.44044230678056806 1106 28-29 0.4554167186402509 1106 30-31 0.4826040412808439 1106 32-33 0.4957166098182597 1106 34-35 0.469679180934655 1106 36-37 0.4574624118032986 1106 38-39 0.4957975175051459 1106 40-41 0.5241669470304728 1106 42-43 0.5740296548552095 1106 44-45 0.566616345706997 1106 46-47 0.49110451071624794 1106 48-49 0.47241232004015643 1106 50-51 0.4426070129405275 1106 52-53 0.47252689451654817 1106 54-55 0.5059754524795252 1106 56-57 0.5322615538419981 1106 58-59 0.5765511113768014 1106 60-61 0.5759922245988278 1106 62-63 0.5209733306190074 1106 64-65 0.5358434085589963 1106 66-67 0.5149954047446883 1106 68-69 0.50814743398373 1106 70-71 0.5111525270104806 1106 72-73 0.459723996242424 1106 74-75 0.5480021378370665 1106 76-77 0.5361697250361495 1106 78-79 0.5189482106911711 1106 80-81 0.5182646705957978 1106 82-83 0.5033353112741388 1106 84-85 0.4865335547050442 1106 86-87 0.5294418278312705 1106 88-89 0.4729884290961657 1106 90-91 0.4278820703860262 1106 92-93 0.5023075193111772 1106 94-95 0.40890981664132653 1106 96-97 0.4731469602668845 1106 98-99 0.5275717991844964 1106 100 0.5048727679421212 1107 1 0.2607883438700114 1107 2 0.3144809540888929 1107 3 0.33347534174065885 1107 4 0.3361883534806438 1107 5 0.34323507297120415 1107 6 0.3218435845234495 1107 7 0.3500392126694152 1107 8 0.3520984301944665 1107 9 0.3961033780710608 1107 10-11 0.36899263857505815 1107 12-13 0.36940692774973627 1107 14-15 0.42974796917477676 1107 16-17 0.47283781911243494 1107 18-19 0.46869929781662734 1107 20-21 0.44286151454635814 1107 22-23 0.4441225530210531 1107 24-25 0.4563346942421447 1107 26-27 0.4667061419232681 1107 28-29 0.49948665914438095 1107 30-31 0.5105334540007931 1107 32-33 0.44906733967871304 1107 34-35 0.45283636240158387 1107 36-37 0.474548319480121 1107 38-39 0.509274064685151 1107 40-41 0.5762159140943055 1107 42-43 0.5176390864482059 1107 44-45 0.4901012137981695 1107 46-47 0.46057728833679334 1107 48-49 0.49847035831242437 1107 50-51 0.511392868547702 1107 52-53 0.5716469164673299 1107 54-55 0.5722682074894934 1107 56-57 0.5139451672582993 1107 58-59 0.4449524180316189 1107 60-61 0.4916852251696895 1107 62-63 0.4670595339429937 1107 64-65 0.4979437861362044 1107 66-67 0.5041415010542991 1107 68-69 0.5393257678274139 1107 70-71 0.4724724246492542 1107 72-73 0.46839469895742525 1107 74-75 0.38827149901361224 1107 76-77 0.4269295352545335 1107 78-79 0.5108113024335843 1107 80-81 0.5494465011092089 1107 82-83 0.4905529568549518 1107 84-85 0.4525156260418193 1107 86-87 0.4542359288016051 1107 88-89 0.5419469171979721 1107 90-91 0.4914528964196805 1107 92-93 0.5125900621850832 1107 94-95 0.47428869500868487 1107 96-97 0.42930621358953047 1107 98-99 0.5028545219583194 1107 100 0.584723930409556 1108 1 0.16587437446464293 1108 2 0.20987970187443494 1108 3 0.3025402965728077 1108 4 0.2251612291893892 1108 5 0.21586351125915826 1108 6 0.23357109621923655 1108 7 0.2890204910776575 1108 8 0.2781543759807832 1108 9 0.3476745728311741 1108 10-11 0.30579471735102715 1108 12-13 0.36356798698350445 1108 14-15 0.3675032159099487 1108 16-17 0.36809426336983364 1108 18-19 0.3574029019851821 1108 20-21 0.4149035324456065 1108 22-23 0.29799328916198675 1108 24-25 0.3716588207111755 1108 26-27 0.33652558685462 1108 28-29 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5751.0 8 7324.0 9 8909.0 10 11690.0 11 17318.0 12 27240.0 13 39593.0 14 42706.0 15 52394.0 16 69379.0 17 97342.0 18 98717.0 19 55121.0 20 44346.0 21 39080.0 22 39625.0 23 45236.0 24 50386.0 25 55066.0 26 60672.0 27 71213.0 28 84637.0 29 101295.0 30 123802.0 31 152779.0 32 192036.0 33 254352.0 34 365939.0 35 587660.0 36 1081669.0 37 2004601.0 38 1823509.0 39 414344.0 40 690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7060555437271 18.14296201423509 13.678425131335686 23.47255731070213 2 15.008277637360884 21.86190346358945 37.491188123432586 25.638630775617077 3 18.559238339380784 27.324444525232682 29.94742445927591 24.16889267611062 4 13.077035419138364 18.224546275161575 35.32985323165138 33.36856507404868 5 13.38957428703208 36.75531806408191 33.50847865365728 16.346628995228738 6 30.365826939303066 35.02024261698358 18.21501852271219 16.398911921001165 7 25.113401429404007 32.87969080703046 22.2031348731634 19.803772890402136 8 27.30094673347086 31.185415494038317 21.25385794471985 20.25977982777097 9 27.607074973250967 14.48000360294337 19.868453297889666 38.044468125916 10-11 24.651697091018487 25.885055222398712 26.343522462525836 23.11972522405696 12-13 26.909962078570647 23.951476775119417 26.717242894795962 22.421318251513974 14-15 25.223510453713953 23.313459058576914 25.796700681473805 25.66632980623533 16-17 22.515881981314696 26.618319051208044 27.35114439255956 23.514654574917696 18-19 23.542863795701866 25.486255956265502 29.528453253824242 21.44242699420839 20-21 24.146977649349438 24.860781291312698 28.72523677228216 22.26700428705571 22-23 23.09125685584023 25.614554460322232 27.123644373504845 24.170544310332698 24-25 22.760973982851564 26.422882594224856 26.804713586569463 24.011429836354115 26-27 23.487927661494133 25.251155428164125 27.087938189509053 24.172978720832692 28-29 22.783966948539362 24.744040813546093 29.22876856674516 23.24322367116939 30-31 24.051397267990733 24.771937101008486 28.428224455503415 22.748441175497362 32-33 21.839087020190853 25.759847346543342 28.461035827496733 23.940029805769072 34-35 21.758178949221126 26.208654504823016 28.823796361995047 23.209370183960814 36-37 23.09780392413697 25.977278226802948 27.63979764003312 23.285120209026967 38-39 23.54015629944434 24.93625453899437 27.494226218930795 24.029362942630495 40-41 22.746829206278218 25.693674913666463 27.64875609704369 23.91073978301163 42-43 24.243271951504664 25.263629442629554 27.22947627485219 23.263622331013597 44-45 23.949005007917727 25.095343214110223 27.61856754446969 23.337084233502363 46-47 22.96847214129128 25.313205043824194 28.5487731182894 23.169549696595123 48-49 23.45908664431004 26.068642773470085 27.744082758090883 22.72818782412899 50-51 22.524324402936152 26.529260741509926 26.584132129713705 24.36228272584022 52-53 22.226814324136686 26.779041259872184 25.477057729384384 25.517086686606742 54-55 22.878523715697582 26.246272460457696 25.462452686240006 25.41275113760471 56-57 23.513047572570304 25.08330533600473 25.82911125443563 25.574535836989337 58-59 23.171799133099505 24.965890459225715 27.39680154606208 24.465508861612705 60-61 23.165804737383304 25.478524170098417 26.661708251329124 24.693962841189155 62-63 21.703362856950314 25.639821275044245 27.16525141635916 25.49156445164628 64-65 21.773114113970152 25.744434906995984 27.111801360273645 25.370649618760215 66-67 23.318252921773546 25.526727248249536 26.113558711469604 25.041461118507318 68-69 23.56818143368566 24.79759899199149 26.168544014679775 25.46567555964308 70-71 22.69882201347602 25.429286228260395 26.321194339817914 25.550697418445672 72-73 24.002878985466715 25.067969912933748 26.235276354148574 24.69387474745096 74-75 24.22403408224661 24.77426922901126 26.413642960367547 24.588053728374582 76-77 23.29506608531561 25.593247848180372 27.021322952730447 24.09036311377357 78-79 23.413750724065746 25.6438578824216 26.904482345066803 24.037909048445847 80-81 22.916373087439236 25.656680161248474 26.574063273604885 24.8528834777074 82-83 23.11620795200796 25.98262600408825 25.522070702161955 25.37909534174183 84-85 23.49882331493829 25.933407587438012 25.29302420758238 25.27474489004132 86-87 23.635782159986864 24.927729418796737 25.90914062961555 25.527347791600853 88-89 23.990999139991697 24.318551834591347 26.50532581347947 25.185123211937487 90-91 24.43010121358528 25.057647892383976 25.77605597941121 24.736194914619535 92-93 23.886189572220772 25.022076958031832 26.10546029813764 24.986273171609756 94-95 23.579913083922836 25.22419804947697 26.362095337270564 24.83379352932963 96-97 24.766646776917213 24.93107676351391 25.42479692696918 24.877479532599697 98-99 25.08473825921797 24.484699276482896 25.17785411725933 25.252708347039803 100 25.027742804123687 24.75394983599116 25.51288667244424 24.705420687440917 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3717.0 1 4285.5 2 3156.0 3 1303.0 4 1029.0 5 1046.5 6 1383.0 7 2287.5 8 3475.5 9 3650.0 10 2914.0 11 2644.0 12 2928.5 13 3315.5 14 3809.5 15 4458.0 16 5189.0 17 5995.5 18 6881.5 19 8024.0 20 9603.5 21 11514.0 22 13885.0 23 16837.5 24 20644.5 25 25209.0 26 31217.0 27 38538.0 28 45653.5 29 54679.5 30 63924.0 31 71788.0 32 80859.5 33 90566.5 34 100054.5 35 107291.0 36 115195.0 37 124670.5 38 132069.0 39 141551.0 40 150099.0 41 157896.0 42 167942.0 43 186597.0 44 213141.0 45 240385.0 46 274694.0 47 316273.0 48 417819.0 49 528789.0 50 627115.5 51 586164.0 52 428539.0 53 360764.5 54 321466.0 55 280920.5 56 249335.5 57 217212.0 58 194657.0 59 176402.5 60 148165.5 61 120987.0 62 96785.5 63 74276.0 64 55040.5 65 43376.5 66 33765.0 67 24968.0 68 19262.0 69 15408.0 70 12860.5 71 10861.0 72 9196.5 73 9175.0 74 7539.0 75 5744.5 76 4545.0 77 3223.0 78 2373.0 79 1626.0 80 1105.5 81 881.0 82 729.5 83 584.0 84 420.0 85 270.5 86 192.5 87 120.5 88 79.5 89 69.0 90 61.0 91 53.5 92 47.0 93 45.0 94 33.0 95 23.5 96 20.5 97 13.0 98 10.0 99 8.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08883666948460403 2 0.28322658604500106 3 0.2883823949577413 4 0.29653861523300273 5 0.3233218791098747 6 0.1706315572000259 7 0.18733588821422495 8 0.15389048645485723 9 0.20395449318949482 10-11 0.22533701661382882 12-13 0.24195562158909867 14-15 0.16903950219134126 16-17 0.1590891583870621 18-19 0.17587921544019036 20-21 0.20754886353602522 22-23 0.17159291349373165 24-25 0.19593298526112213 26-27 0.19426132750200323 28-29 0.22352452321932628 30-31 0.2575271442317337 32-33 0.22615141398365596 34-35 0.1524943766780107 36-37 0.14644456764500896 38-39 0.11364823446610496 40-41 0.10434083595379465 42-43 0.12094719435207463 44-45 0.13544714150809492 46-47 0.2014868079260336 48-49 0.206740589454693 50-51 0.21312105606642154 52-53 0.1402233065341489 54-55 0.083858435938217 56-57 0.12255149593774918 58-59 0.14291755347192295 60-61 0.11898161874519858 62-63 0.14026616955361348 64-65 0.1150443442429515 66-67 0.11895100230272387 68-69 0.10376524683527019 70-71 0.12999741474759743 72-73 0.15522536334675435 74-75 0.19410212200113477 76-77 0.1737789274864256 78-79 0.19903749252805722 80-81 0.10192213699829296 82-83 0.14321759460817507 84-85 0.17461169472173757 86-87 0.1891667514742123 88-89 0.170000858485047 90-91 0.17294003696261867 92-93 0.12228207124397178 94-95 0.3659644601886438 96-97 0.36575014509132087 98-99 0.4132240007925984 100 0.4459223613555834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8165547.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.82333906665349 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33655730613026 28.84613610997966 2 12.273921221010923 9.039335255973576 3 3.635085666617144 4.015679761145257 4 1.5536265984185202 2.2883887606614666 5 0.8364001425130166 1.5399523021577055 6 0.5368201015961449 1.186050517132213 7 0.3867756807662325 0.9969660424913495 8 0.2865224040094643 0.8440569306426546 9 0.22566014820548072 0.7478604136081524 >10 1.544614571334383 11.280698585793317 >50 0.1929576094276497 5.002874392982083 >100 0.15971116047696374 12.00377150043167 >500 0.019049149563655783 4.7510415706898055 >1k 0.01072754080920483 6.893695735208774 >5k 7.352208652078096E-4 1.9891605534805037 >10k+ 8.354782559179653E-4 8.574331567621883 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 88101 1.0789356793855942 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 80064 0.9805099401179126 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 43491 0.5326158798669581 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 39211 0.4802005303502631 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 37711 0.4618306648654401 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 34685 0.4247725228940572 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 34580 0.4234866323101195 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33274 0.40749260276133364 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 29211 0.35773476045144315 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 28612 0.35039906083450384 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 28448 0.34839062220816314 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 26333 0.3224891118745627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22734 0.2784136812879774 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 21974 0.26910628277566706 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 16763 0.20528937008139195 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 16476 0.20177460248529586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16190 0.1982720814661896 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 15445 0.1891483816087275 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 14199 0.17388914667933453 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 12734 0.1559479113891574 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 12130 0.14855097888726868 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 11989 0.1468242115316953 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 10996 0.13466336058074246 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 10777 0.13198136021995832 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 10630 0.13018111340244568 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 9895 0.1211798793148824 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 9842 0.12053081073441865 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9832 0.12040834496451984 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 9689 0.1186570844549667 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8819 0.10800256247376937 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.32684962807758E-4 0.0 1.2246576989882001E-5 0.0 1.2246576989882001E-5 2 4.2863019464587E-4 0.0 1.2246576989882001E-5 0.0 1.2246576989882001E-5 3 4.2863019464587E-4 0.0 1.2246576989882001E-5 0.0 1.2246576989882001E-5 4 4.8986307959528E-4 0.0 1.2246576989882001E-5 1.2246576989882001E-5 1.2246576989882001E-5 5 0.0020574249343001763 0.0 1.2246576989882001E-5 3.6739730969646E-5 1.2246576989882001E-5 6 0.003588247058035426 0.0 1.2246576989882001E-5 4.8986307959528004E-5 1.2246576989882001E-5 7 0.003967890944721768 0.0 1.2246576989882001E-5 6.123288494941E-5 1.2246576989882001E-5 8 0.004359781408397993 0.0 1.2246576989882001E-5 7.3479461939292E-5 1.2246576989882001E-5 9 0.004690438987124806 0.0 1.2246576989882001E-5 1.10219192908938E-4 1.2246576989882001E-5 10-11 0.007574507868242018 0.0 1.2246576989882001E-5 1.77575366353289E-4 1.8369865484823003E-5 12-13 0.01486734446571675 0.0 1.2246576989882001E-5 2.20438385817876E-4 3.6739730969646E-5 14-15 0.02322563326131121 1.2246576989882001E-5 1.2246576989882001E-5 3.1228771324199104E-4 4.2863019464587005E-5 16-17 0.02648934602911477 1.2246576989882001E-5 1.2246576989882001E-5 4.2863019464587E-4 4.8986307959528004E-5 18-19 0.028454921635990827 1.2246576989882001E-5 1.2246576989882001E-5 5.878356955143361E-4 7.3479461939292E-5 20-21 0.036788717277605534 1.2246576989882001E-5 1.2246576989882001E-5 0.001120561794574203 7.3479461939292E-5 22-23 0.04000956702594449 2.4493153979764002E-5 1.2246576989882001E-5 0.0035025210191062524 7.3479461939292E-5 24-25 0.05941426826641252 3.0616442474705E-5 1.2246576989882001E-5 0.009319645089300202 7.3479461939292E-5 26-27 0.12171260541394226 3.6739730969646E-5 1.2246576989882001E-5 0.019741482107689785 7.3479461939292E-5 28-29 0.1348470592355907 3.6739730969646E-5 1.2246576989882001E-5 0.0637556798093257 7.3479461939292E-5 30-31 0.13688611430440606 3.6739730969646E-5 1.2246576989882001E-5 0.1866378333258017 7.3479461939292E-5 32-33 0.13830059394673744 3.6739730969646E-5 1.2246576989882001E-5 0.3691975565139727 9.797261591905601E-5 34-35 0.13965996399261432 4.8986307959528004E-5 1.2246576989882001E-5 0.6052564512824432 9.797261591905601E-5 36-37 0.14203579992865145 6.123288494941E-5 1.2246576989882001E-5 0.9492383057742488 9.797261591905601E-5 38-39 0.14449736190361773 6.123288494941E-5 1.2246576989882001E-5 1.4270874933424547 1.10219192908938E-4 40-41 0.1469283074361093 7.3479461939292E-5 1.2246576989882001E-5 2.0588087975000327 1.2246576989882E-4 42-43 0.1515758834037695 7.3479461939292E-5 1.2246576989882001E-5 2.5226417777033188 1.34712346888702E-4 44-45 0.15704398002975184 7.3479461939292E-5 1.2246576989882001E-5 3.0019911709527847 1.34712346888702E-4 46-47 0.15994641877635385 7.3479461939292E-5 1.2246576989882001E-5 3.5412079558172893 1.34712346888702E-4 48-49 0.16185688478677546 7.3479461939292E-5 1.2246576989882001E-5 4.030458706563076 1.4083563538364301E-4 50-51 0.16452663857056973 7.3479461939292E-5 1.2246576989882001E-5 4.556467558143992 1.46958923878584E-4 52-53 0.16561046063417428 7.3479461939292E-5 1.2246576989882001E-5 5.1443338701008035 1.59205500868466E-4 54-55 0.16835981716840281 7.3479461939292E-5 1.2246576989882001E-5 5.662670241197558 1.59205500868466E-4 56-57 0.1745872015677578 7.3479461939292E-5 1.2246576989882001E-5 6.207893972075601 1.59205500868466E-4 58-59 0.17703039367723927 7.3479461939292E-5 1.2246576989882001E-5 6.755591511505598 1.65328789363407E-4 60-61 0.178199941779773 7.3479461939292E-5 1.2246576989882001E-5 7.258472702441122 1.71452077858348E-4 62-63 0.1789898459956204 7.3479461939292E-5 1.2246576989882001E-5 7.777623470907706 1.71452077858348E-4 64-65 0.17978587349996272 7.3479461939292E-5 1.2246576989882001E-5 8.330054312344291 1.71452077858348E-4 66-67 0.18087581885206222 7.3479461939292E-5 2.4493153979764002E-5 8.880740016559821 1.77575366353289E-4 68-69 0.18188003816523252 7.3479461939292E-5 2.4493153979764002E-5 9.509552758682302 2.0206852033305303E-4 70-71 0.18317205203766507 7.3479461939292E-5 4.2863019464587005E-5 10.232364102490623 2.20438385817876E-4 72-73 0.18622757299664064 7.3479461939292E-5 6.123288494941E-5 10.832770909285074 2.32684962807758E-4 74-75 0.18911776516625278 7.3479461939292E-5 6.123288494941E-5 11.412278932446288 2.32684962807758E-4 76-77 0.190134231056413 8.5726038929174E-5 6.123288494941E-5 12.03810350978324 2.32684962807758E-4 78-79 0.1908506558103211 8.5726038929174E-5 6.123288494941E-5 12.645913372368074 2.4493153979764E-4 80-81 0.1921365463942587 8.5726038929174E-5 6.123288494941E-5 13.30871648892597 2.4493153979764E-4 82-83 0.19262640947385398 8.5726038929174E-5 6.123288494941E-5 14.052463356098496 2.5717811678752203E-4 84-85 0.194702204273639 8.5726038929174E-5 6.123288494941E-5 14.73599380421177 2.6330140528246304E-4 86-87 0.1986945883723405 8.5726038929174E-5 6.123288494941E-5 15.406402045080384 2.69424693777404E-4 88 0.199680437820026 8.5726038929174E-5 6.123288494941E-5 15.929967704551819 2.69424693777404E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 8585 0.0 27.861876 1 ATCTACA 9945 0.0 24.076252 2 TCTACAC 11530 0.0 23.252129 3 TACACTC 11465 0.0 22.606712 5 CTACACT 13635 0.0 21.21784 4 AGTACTT 37545 0.0 20.935558 12-13 GAGTACT 36200 0.0 20.551468 12-13 GTATATA 1575 0.0 19.133146 1 CACTCTT 14070 0.0 18.742489 7 GTACTAG 2575 0.0 18.468477 1 ACACTCT 14710 0.0 18.118776 6 GTACTTT 42130 0.0 17.754732 14-15 TACTTTT 44890 0.0 17.667917 14-15 GTATAAG 2720 0.0 17.483946 1 TATTCGC 485 0.0 17.445625 9 ACTCTTT 16500 0.0 17.349693 8 CATGGGG 17235 0.0 16.94968 4 CGTTAAC 860 0.0 16.94412 9 AGTACTC 2630 0.0 16.63363 5 GTATTAG 2385 0.0 16.38614 1 >>END_MODULE