##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765379_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8028772 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17821641964674 33.0 31.0 34.0 31.0 34.0 2 32.20108180927295 34.0 31.0 34.0 30.0 34.0 3 32.21421520003308 34.0 31.0 34.0 30.0 34.0 4 35.698750942236245 37.0 35.0 37.0 35.0 37.0 5 35.73222692087906 37.0 35.0 37.0 35.0 37.0 6 35.827192751270054 37.0 37.0 37.0 35.0 37.0 7 35.78466495249834 37.0 36.0 37.0 35.0 37.0 8 35.81553555138943 37.0 36.0 37.0 35.0 37.0 9 37.49399858409231 39.0 38.0 39.0 35.0 39.0 10-11 37.4259128917847 39.0 38.0 39.0 35.0 39.0 12-13 37.400949672004636 39.0 38.0 39.0 35.0 39.0 14-15 38.75653213218659 41.0 38.5 41.0 35.0 41.0 16-17 38.726158869625394 40.0 38.0 41.0 35.0 41.0 18-19 38.7305828338381 40.0 38.5 41.0 35.0 41.0 20-21 38.71363771445995 40.5 39.0 41.0 35.0 41.0 22-23 38.59636573314076 40.0 38.5 41.0 34.0 41.0 24-25 38.50364601211742 40.0 38.0 41.0 34.0 41.0 26-27 38.37376101849698 40.0 38.0 41.0 34.0 41.0 28-29 38.227925453606105 40.0 38.0 41.0 34.0 41.0 30-31 37.987317487655645 40.0 38.0 41.0 33.5 41.0 32-33 37.7262684131521 40.0 37.0 41.0 33.0 41.0 34-35 37.550694925699716 40.0 37.0 41.0 33.0 41.0 36-37 37.358970089074646 40.0 37.0 41.0 32.5 41.0 38-39 37.14347654909119 40.0 36.0 41.0 32.0 41.0 40-41 36.86945936439595 40.0 36.0 41.0 31.0 41.0 42-43 36.57111884607011 40.0 35.5 41.0 30.5 41.0 44-45 36.17558519534494 39.0 35.0 41.0 30.0 41.0 46-47 35.68185234304823 39.0 35.0 41.0 28.5 41.0 48-49 35.21309840408969 39.0 35.0 41.0 26.0 41.0 50-51 33.90992825552899 38.0 33.5 40.0 23.5 40.5 52-53 33.81451067485787 37.5 33.5 39.5 23.0 40.0 54-55 34.58377725759307 38.0 35.0 40.0 24.0 41.0 56-57 34.58022453496002 38.0 35.0 41.0 24.0 41.0 58-59 34.346293368400545 38.0 35.0 40.0 23.0 41.0 60-61 34.004092468935475 37.0 34.5 40.0 22.5 41.0 62-63 33.58084860299931 36.5 34.0 40.0 21.0 41.0 64-65 33.24839103663673 36.0 34.0 39.0 20.0 41.0 66-67 32.95208090353045 35.5 34.0 39.0 20.0 41.0 68-69 32.70202989946657 35.0 34.0 39.0 18.5 41.0 70-71 32.3614106241901 35.0 34.0 37.5 17.0 40.5 72-73 32.00466036400087 35.0 33.5 37.0 13.0 39.0 74-75 31.62965743702773 35.0 33.0 36.5 7.5 39.0 76-77 31.297593766518716 35.0 33.0 36.0 4.5 38.5 78-79 31.02270745762864 35.0 33.0 36.0 2.0 37.0 80-81 30.74191046899825 35.0 33.0 35.0 2.0 37.0 82-83 30.497039896013987 35.0 33.0 35.0 2.0 36.5 84-85 30.265285151951005 35.0 32.0 35.0 2.0 36.0 86-87 30.02574889410236 35.0 32.0 35.0 2.0 36.0 88-89 29.83107211165045 35.0 32.0 35.0 2.0 36.0 90-91 29.610075550781612 35.0 32.0 35.0 2.0 35.0 92-93 29.36210487730876 35.0 31.0 35.0 2.0 35.0 94-95 29.08202474799384 35.0 31.0 35.0 2.0 35.0 96-97 28.817540403439033 35.0 31.0 35.0 2.0 35.0 98-99 28.523402084403443 35.0 31.0 35.0 2.0 35.0 100 28.017391077988016 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4913717205690489 1101 2 0.5922188434290945 1101 3 0.5895873992423901 1101 4 0.45290390411261683 1101 5 0.49406214057974296 1101 6 0.46917659583754556 1101 7 0.5387847907715582 1101 8 0.4984742656790502 1101 9 0.6523351766047156 1101 10-11 0.6219485857075426 1101 12-13 0.5816493503582407 1101 14-15 0.8376701208919926 1101 16-17 0.8351559640338238 1101 18-19 0.8322107505312104 1101 20-21 0.8272304063801457 1101 22-23 0.7567153514807643 1101 24-25 0.8098990452604298 1101 26-27 0.8361681234902463 1101 28-29 0.8073879736208127 1101 30-31 0.8271132188242731 1101 32-33 0.892666914529066 1101 34-35 1.0074621849981682 1101 36-37 1.0259167667656754 1101 38-39 0.9409919970741143 1101 40-41 1.0421526804234915 1101 42-43 1.0094421930022222 1101 44-45 1.0828580512915522 1101 46-47 1.2486790464792676 1101 48-49 1.3684944492925197 1101 50-51 1.3250338288636243 1101 52-53 1.3669690301972395 1101 54-55 1.48570586886634 1101 56-57 1.5342347815208157 1101 58-59 1.528618517226377 1101 60-61 1.5383778916687376 1101 62-63 1.5625241934284162 1101 64-65 1.540368619914716 1101 66-67 1.5141984092475056 1101 68-69 1.558119089095932 1101 70-71 1.5139878636188087 1101 72-73 1.4757493230719163 1101 74-75 1.4702641809310144 1101 76-77 1.3684280781955103 1101 78-79 1.449300633814108 1101 80-81 1.498337228196302 1101 82-83 1.4710257837665068 1101 84-85 1.450111557260957 1101 86-87 1.4704758100021422 1101 88-89 1.5242468572861085 1101 90-91 1.5215505172035755 1101 92-93 1.5313146232063133 1101 94-95 1.4367635153666782 1101 96-97 1.4630565845870613 1101 98-99 1.4767473180861792 1101 100 1.4923907867775767 1102 1 0.4430514834946777 1102 2 0.5341830424507492 1102 3 0.598645330662201 1102 4 0.4169008266051861 1102 5 0.4754089973155473 1102 6 0.4111348057756672 1102 7 0.4937191150259821 1102 8 0.5029693191756976 1102 9 0.5424621983255165 1102 10-11 0.5998559714012828 1102 12-13 0.6163444223925296 1102 14-15 0.8258815173920055 1102 16-17 0.8064245932272271 1102 18-19 0.734787054631461 1102 20-21 0.7590159894115871 1102 22-23 0.7832343392737968 1102 24-25 0.786969942657187 1102 26-27 0.783456527030026 1102 28-29 0.8120285022157674 1102 30-31 0.8580467056440781 1102 32-33 0.8892600852885977 1102 34-35 0.9042480435015321 1102 36-37 0.8687448068251271 1102 38-39 0.9085789473537105 1102 40-41 0.899522954216657 1102 42-43 0.8808279204457747 1102 44-45 1.0426946089426394 1102 46-47 1.078660787552515 1102 48-49 1.071466914416149 1102 50-51 1.0572302215426888 1102 52-53 1.0464681768824704 1102 54-55 1.168955789303574 1102 56-57 1.2083836154319982 1102 58-59 1.2792805896696606 1102 60-61 1.2890592795545857 1102 62-63 1.2479578866901093 1102 64-65 1.2716601813349087 1102 66-67 1.2683933020630036 1102 68-69 1.184755395516632 1102 70-71 1.1177739755563323 1102 72-73 1.0665007910131408 1102 74-75 1.0651763637251044 1102 76-77 1.1040616603610083 1102 78-79 1.1278675964996587 1102 80-81 1.1719341071319 1102 82-83 1.1653953192949302 1102 84-85 1.2104236248105593 1102 86-87 1.1945134504847132 1102 88-89 1.0968899811505963 1102 90-91 1.1044133639497957 1102 92-93 1.1282914842135554 1102 94-95 1.0949251519161614 1102 96-97 1.1480995873735402 1102 98-99 1.2092843496137817 1102 100 1.1947088602631126 1103 1 0.4128434125253193 1103 2 0.4502486108372281 1103 3 0.5083945274670398 1103 4 0.43680376089548645 1103 5 0.44782476272219895 1103 6 0.45756803181586037 1103 7 0.45873149224542686 1103 8 0.4397334886808082 1103 9 0.5114185869852363 1103 10-11 0.5306790293180654 1103 12-13 0.5596256379527418 1103 14-15 0.6112924265064592 1103 16-17 0.6830008181880203 1103 18-19 0.6877612300366565 1103 20-21 0.6634418601246352 1103 22-23 0.6902905223442417 1103 24-25 0.7113613102665042 1103 26-27 0.6716323101438562 1103 28-29 0.671598557185284 1103 30-31 0.7234756850594763 1103 32-33 0.788823800734896 1103 34-35 0.8186893201117513 1103 36-37 0.810792856756791 1103 38-39 0.8529725630537044 1103 40-41 0.9044535814223877 1103 42-43 0.900823785069079 1103 44-45 0.8935908153821259 1103 46-47 0.8679611479640101 1103 48-49 0.9830032508008273 1103 50-51 0.8898325090905956 1103 52-53 0.9321902915956954 1103 54-55 1.009873849867411 1103 56-57 1.0246831055178447 1103 58-59 1.0895456215210757 1103 60-61 1.060041748379092 1103 62-63 1.1206413414134104 1103 64-65 1.1518789261093048 1103 66-67 1.103336205879181 1103 68-69 1.063126068664836 1103 70-71 1.008589484329093 1103 72-73 0.9410853394603009 1103 74-75 1.018977764803683 1103 76-77 0.9856752274495157 1103 78-79 0.9625804873338062 1103 80-81 0.9929691239197247 1103 82-83 0.9845360537036001 1103 84-85 0.9430203152602665 1103 86-87 0.93190544325752 1103 88-89 1.0188464838338476 1103 90-91 1.048112580980515 1103 92-93 1.0070418580280176 1103 94-95 1.026275851981314 1103 96-97 1.0710209072063002 1103 98-99 1.1227903557395962 1103 100 1.1223525351142634 1104 1 0.3584936747108145 1104 2 0.3932767211837458 1104 3 0.4451239512052325 1104 4 0.41241293913946464 1104 5 0.43600233750488826 1104 6 0.3987725115627683 1104 7 0.45190196239796165 1104 8 0.4443522998888412 1104 9 0.5470106761146454 1104 10-11 0.5400221672191421 1104 12-13 0.5161753172201031 1104 14-15 0.5885165795175737 1104 16-17 0.5887392395409847 1104 18-19 0.5947533380484131 1104 20-21 0.5779299615762596 1104 22-23 0.6016902324587079 1104 24-25 0.5859655678192937 1104 26-27 0.6188138828768004 1104 28-29 0.5992834571890242 1104 30-31 0.6510059932988881 1104 32-33 0.658511434521948 1104 34-35 0.7125869979991606 1104 36-37 0.7317238453543524 1104 38-39 0.7085313646305593 1104 40-41 0.6514270327288045 1104 42-43 0.6914257221124913 1104 44-45 0.7427862969204782 1104 46-47 0.7699445659413016 1104 48-49 0.8251309465077057 1104 50-51 0.7165069068299417 1104 52-53 0.8203321502476939 1104 54-55 0.846337347269305 1104 56-57 0.8120850791383774 1104 58-59 0.7729967319869431 1104 60-61 0.761509523997475 1104 62-63 0.7616106231658719 1104 64-65 0.7811101594054151 1104 66-67 0.6879253931843081 1104 68-69 0.6580930631598605 1104 70-71 0.6912053183022238 1104 72-73 0.6657577931495737 1104 74-75 0.6264320448905885 1104 76-77 0.6313437485048148 1104 78-79 0.6228469005732968 1104 80-81 0.671384675607765 1104 82-83 0.6139111015774645 1104 84-85 0.6822716600334502 1104 86-87 0.685086910296846 1104 88-89 0.6707752736478341 1104 90-91 0.6342603929147508 1104 92-93 0.6700951982729997 1104 94-95 0.671843548448237 1104 96-97 0.7137205954101198 1104 98-99 0.7574605352346779 1104 100 0.8291112858631742 1105 1 0.2356528926924497 1105 2 0.306847852432945 1105 3 0.35957069008672704 1105 4 0.292904667959732 1105 5 0.3583093215064821 1105 6 0.34435614765025235 1105 7 0.3241326753924483 1105 8 0.3704869073488197 1105 9 0.39579043903393796 1105 10-11 0.4220100754003795 1105 12-13 0.43923435222011875 1105 14-15 0.5102063797597722 1105 16-17 0.47448177347306597 1105 18-19 0.48733626016045406 1105 20-21 0.4893959129540022 1105 22-23 0.3860888058838725 1105 24-25 0.438992358733735 1105 26-27 0.44516356905180743 1105 28-29 0.4345003450796625 1105 30-31 0.4264190727576178 1105 32-33 0.4478093242757595 1105 34-35 0.41144898953924525 1105 36-37 0.41589891183934213 1105 38-39 0.38460079277366077 1105 40-41 0.45900400713045286 1105 42-43 0.40717022523656965 1105 44-45 0.3880616642884078 1105 46-47 0.3822607256061872 1105 48-49 0.33925304495959097 1105 50-51 0.2551888898281689 1105 52-53 0.2979937311033538 1105 54-55 0.403866238568348 1105 56-57 0.44058960760238364 1105 58-59 0.41158568134847684 1105 60-61 0.44059591273182264 1105 62-63 0.551215436876042 1105 64-65 0.4514287442308387 1105 66-67 0.4599775810462674 1105 68-69 0.4609455143869141 1105 70-71 0.4652315976854311 1105 72-73 0.4499933531292939 1105 74-75 0.46032624350918994 1105 76-77 0.4838445180950721 1105 78-79 0.519284211101116 1105 80-81 0.4415558889813447 1105 82-83 0.46627007162229006 1105 84-85 0.4522247403073685 1105 86-87 0.4267857600656342 1105 88-89 0.48096374690065247 1105 90-91 0.5109494650041704 1105 92-93 0.5196637586268587 1105 94-95 0.5161245572541446 1105 96-97 0.43889428617108805 1105 98-99 0.4451258616206921 1105 100 0.5995113695929248 1106 1 0.23365652229230705 1106 2 0.33752570066096865 1106 3 0.355076279671799 1106 4 0.35156524337170936 1106 5 0.3537625306441896 1106 6 0.3003604171898857 1106 7 0.30405849097798665 1106 8 0.329614324709695 1106 9 0.3202369269944185 1106 10-11 0.41228477772530425 1106 12-13 0.41333107386331136 1106 14-15 0.4633230842366558 1106 16-17 0.48293523769482505 1106 18-19 0.4662232561149793 1106 20-21 0.4405980005975749 1106 22-23 0.47097532773233297 1106 24-25 0.4846504655757613 1106 26-27 0.47423038730833156 1106 28-29 0.4870387416278916 1106 30-31 0.44919343530437317 1106 32-33 0.4288124773783366 1106 34-35 0.44832605240308254 1106 36-37 0.5055534115718103 1106 38-39 0.5653461830196207 1106 40-41 0.6186423067312319 1106 42-43 0.48239585053987355 1106 44-45 0.41293336127466773 1106 46-47 0.38741065098425054 1106 48-49 0.39593151645354396 1106 50-51 0.387443799905391 1106 52-53 0.39285754872607725 1106 54-55 0.39298417639310657 1106 56-57 0.37799435904212686 1106 58-59 0.3613949693421219 1106 60-61 0.3941319354537498 1106 62-63 0.3897722737975826 1106 64-65 0.34213947742719597 1106 66-67 0.3092802134271082 1106 68-69 0.30335643418175096 1106 70-71 0.3234230077961584 1106 72-73 0.33629354602247474 1106 74-75 0.37199185384222133 1106 76-77 0.37464274053340674 1106 78-79 0.31380452156303207 1106 80-81 0.3674407711699281 1106 82-83 0.34527408181246955 1106 84-85 0.33882772837761976 1106 86-87 0.40156948633634215 1106 88-89 0.39912398337462207 1106 90-91 0.39527881529080133 1106 92-93 0.4470624874461393 1106 94-95 0.4310453005640369 1106 96-97 0.39712196190555105 1106 98-99 0.49135756829070587 1106 100 0.5431833882423049 1107 1 0.20656799792748615 1107 2 0.2287976444523423 1107 3 0.26042592545629617 1107 4 0.19742981140128535 1107 5 0.2774334999270778 1107 6 0.1778051042258113 1107 7 0.26966179390081635 1107 8 0.20059229962891578 1107 9 0.3078666840881752 1107 10-11 0.36701620588026174 1107 12-13 0.312286293607805 1107 14-15 0.3037495937582477 1107 16-17 0.3803948666000494 1107 18-19 0.41697942310768354 1107 20-21 0.37198813708381806 1107 22-23 0.32639938987351513 1107 24-25 0.34268158756248823 1107 26-27 0.3517477571779821 1107 28-29 0.3734078673884511 1107 30-31 0.38475539553552807 1107 32-33 0.36278048154954234 1107 34-35 0.33182859344866245 1107 36-37 0.3830449159358622 1107 38-39 0.3647123240076624 1107 40-41 0.320514856413304 1107 42-43 0.32750049154100225 1107 44-45 0.3077544491909592 1107 46-47 0.24154380312780432 1107 48-49 0.2577494400189764 1107 50-51 0.1706642039834918 1107 52-53 0.2688348299309098 1107 54-55 0.33440910663865253 1107 56-57 0.39673238422863477 1107 58-59 0.3055957948042547 1107 60-61 0.325540429582027 1107 62-63 0.3799794865593853 1107 64-65 0.3758931534572696 1107 66-67 0.3276867023454102 1107 68-69 0.34058557961046887 1107 70-71 0.3736279541598009 1107 72-73 0.2749937627067425 1107 74-75 0.2828577955666738 1107 76-77 0.26229894606037973 1107 78-79 0.27753700832676387 1107 80-81 0.30751842140151453 1107 82-83 0.24665525708366687 1107 84-85 0.30890031598965706 1107 86-87 0.26291476547412884 1107 88-89 0.21862868656283396 1107 90-91 0.19160880371831368 1107 92-93 0.27367403967984316 1107 94-95 0.20317791775705274 1107 96-97 0.21421116258848727 1107 98-99 0.15309201112757265 1107 100 0.21454369043198085 1108 1 0.13640940087388742 1108 2 0.12804039220552 1108 3 0.1391057475133337 1108 4 0.19445061572861277 1108 5 0.27100863370308304 1108 6 0.22618589691417412 1108 7 0.27894790085225196 1108 8 0.26571754052762486 1108 9 0.35154682610516375 1108 10-11 0.35093293236137413 1108 12-13 0.31815956297909764 1108 14-15 0.34885391729375925 1108 16-17 0.37519335893821193 1108 18-19 0.39617537477297304 1108 20-21 0.3528598037186228 1108 22-23 0.3632405091583166 1108 24-25 0.37630323285439005 1108 26-27 0.3483351765642979 1108 28-29 0.365050261003681 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-0.89254209594149 2316 86-87 -0.8699871320925325 2316 88-89 -0.8136185255634985 2316 90-91 -0.7725557775067351 2316 92-93 -0.7160865654513557 2316 94-95 -0.6320758739437515 2316 96-97 -0.7175826110656587 2316 98-99 -0.8528971697643009 2316 100 -0.9503284345848719 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22376.0 3 3973.0 4 3846.0 5 4529.0 6 5518.0 7 6361.0 8 7904.0 9 9705.0 10 12631.0 11 18606.0 12 28200.0 13 40050.0 14 43174.0 15 53024.0 16 71846.0 17 99706.0 18 99226.0 19 56253.0 20 47004.0 21 42159.0 22 43655.0 23 49912.0 24 56229.0 25 60693.0 26 66943.0 27 77220.0 28 88977.0 29 104772.0 30 126984.0 31 156396.0 32 199903.0 33 265463.0 34 383262.0 35 618123.0 36 1136297.0 37 1970833.0 38 1617512.0 39 329141.0 40 366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.868701275559545 18.174675330424325 13.290731135172301 23.66589225884383 2 15.648800088011882 21.64790996784566 37.190208178104044 25.513081766038415 3 18.65334279382484 26.990307163821747 29.470297911711107 24.8860521306423 4 13.31549832037236 17.651336832073117 35.33199166493613 33.70117318261839 5 13.93199185729324 36.55758751198431 32.99968841428357 16.510732216438882 6 30.865940112949254 35.255694857416536 17.816958851350005 16.06140617828421 7 25.959942239304716 32.37793587345338 21.92608666798727 19.73603521925463 8 27.38893625130136 30.966554044447946 20.92115679796392 20.723352906286774 9 27.695059896737895 14.304061675186553 19.80522061862155 38.195657809454 10-11 25.19569917381861 25.386237366204522 26.132801101142505 23.285262358834363 12-13 27.07120123061667 23.571425985679838 26.49215579239548 22.865216991308017 14-15 25.16664384977776 23.123109225746557 25.432438256407153 26.27780866806853 16-17 22.977131973337546 26.272123586833352 26.5436957852586 24.207048654570503 18-19 23.780327804025887 25.085205607119303 29.0087083957033 22.125758193151505 20-21 24.722612117770687 24.35263894280619 28.084274072425604 22.840474866997525 22-23 23.766903052405077 24.889304905166256 27.044702953399526 24.299089089029145 24-25 23.307426308279187 25.675947391901165 26.839283676715116 24.177342623104533 26-27 23.823936029456075 24.94739674884772 27.05509502021724 24.17357220147897 28-29 23.178495147969038 24.49394116873469 28.740263684369317 23.587299998926955 30-31 24.190263047095232 24.513100551968126 28.025916625463683 23.270719775472962 32-33 22.62261183337464 25.328404409269623 27.769004460484464 24.27997929687127 34-35 22.73732881312303 25.680229667191345 28.167018802573292 23.41542271711233 36-37 23.48991782567914 25.704283646267946 27.201045342732094 23.60475318532082 38-39 23.884565476311796 24.824391164843217 27.07850560572684 24.212537753118152 40-41 23.327883353477816 25.454915004248292 27.157778163381717 24.05942347889217 42-43 24.59992404137763 25.149832558228812 26.67028261618248 23.579960784211078 44-45 24.214021233465452 25.148646289124237 26.890823248882413 23.746509228527895 46-47 23.564695630188698 25.244487024876562 27.467114484644384 23.72370286029036 48-49 24.089182512773608 25.877955818188152 26.667066540854012 23.365795128184228 50-51 23.363023492595865 26.2674185595001 25.670221500192152 24.699336447711882 52-53 23.194552154867594 26.33625781289794 24.986522472448456 25.482667559786016 54-55 23.77603273994999 25.884543146627692 24.95209191323709 25.38733220018523 56-57 24.14105359173448 25.001237095720548 25.256091278593583 25.60161803395139 58-59 23.789521316122087 24.89785479960401 26.566044045677508 24.7465798385964 60-61 23.717319704847732 25.31580872707839 26.048036073584495 24.91883549448938 62-63 22.693103718683656 25.396457913268904 26.347182696219257 25.563255671828184 64-65 22.62297448914419 25.29333877702575 26.432910823752476 25.650775910077584 66-67 23.786614424696126 25.069849593420162 25.831847424886323 25.311688556997385 68-69 23.824564098031395 24.45179319354268 25.929558306699608 25.79408440172632 70-71 23.108155135391193 24.81125059385111 26.09366345354686 25.986930817210833 72-73 24.19312326885787 24.456490757782618 26.056616494599744 25.29376947875977 74-75 24.30911223643678 24.194750739921002 26.212456180821448 25.283680842820765 76-77 23.434668920953573 24.89421486633828 26.654615266828625 25.016500945879518 78-79 23.547182135239645 24.78870598696309 26.53824361758104 25.125868260216222 80-81 23.14645244165943 24.74316037653522 26.36082488878428 25.74956229302107 82-83 23.37114386487059 24.98796329167197 25.614876093148563 26.026016750308877 84-85 23.580427428536783 24.855768022221557 25.369315015139748 26.194489534101912 86-87 23.581185118587094 24.13251999928633 25.918179253643615 26.368115628482965 88-89 23.886688238275312 23.56922693072395 26.343793224435696 26.200291606565045 90-91 24.261111826858798 24.033932471337422 25.820727768781538 25.884227933022235 92-93 23.75618996892766 24.193100349373704 26.01396547257213 26.036744209126507 94-95 23.382619923485276 24.22022263043906 26.40222889141634 25.994928554659325 96-97 24.386134850273464 24.016704388329472 25.55359110042461 26.043569660972455 98-99 24.477841061435036 23.67335155387541 25.468143089456508 26.380664295233046 100 24.66091183707764 23.665390501530396 25.698569127973276 25.97512853341869 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1862.0 1 2242.5 2 1833.0 3 1003.0 4 846.0 5 838.5 6 1088.5 7 1848.0 8 2818.5 9 2993.5 10 2496.0 11 2291.0 12 2552.0 13 2947.5 14 3410.5 15 3953.0 16 4660.5 17 5384.0 18 6295.0 19 7414.5 20 8672.0 21 10187.5 22 12115.5 23 14654.5 24 17526.0 25 21046.5 26 26016.0 27 31619.5 28 37402.0 29 46013.0 30 56241.5 31 65479.0 32 75661.0 33 86373.5 34 97725.5 35 106263.0 36 113087.5 37 123339.5 38 130820.0 39 138496.5 40 143327.5 41 145653.0 42 153411.0 43 172359.0 44 198633.5 45 224678.0 46 257694.0 47 293826.0 48 376977.5 49 457806.0 50 516367.0 51 492378.5 52 399645.5 53 370582.0 54 346403.5 55 310100.0 56 283909.0 57 254606.5 58 231954.0 59 213137.5 60 184178.0 61 153096.5 62 125242.5 63 99823.0 64 75053.5 65 57842.0 66 44393.0 67 32235.5 68 24587.0 69 19876.0 70 16651.0 71 14133.0 72 11987.0 73 11841.5 74 9827.5 75 7739.5 76 5979.5 77 3941.0 78 2751.0 79 1946.0 80 1461.0 81 1133.0 82 835.0 83 595.0 84 409.0 85 286.0 86 202.5 87 129.5 88 95.5 89 79.5 90 66.0 91 59.5 92 53.5 93 46.5 94 38.0 95 32.5 96 32.0 97 21.0 98 18.0 99 14.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1159704124117611 2 0.37181277535344137 3 0.3810919029709649 4 0.37705641659770633 5 0.3058251000277502 6 0.13715671587136863 7 0.15255134907305876 8 0.1273420144450484 9 0.20841294285103623 10-11 0.20837557723646905 12-13 0.22206384737292328 14-15 0.15007899090919508 16-17 0.13489609619005247 18-19 0.15478705834466341 20-21 0.15419543611401593 22-23 0.16266497541591665 24-25 0.17624737631109713 26-27 0.16917904755546675 28-29 0.17673935690289871 30-31 0.21947316476293013 32-33 0.21700080659906645 34-35 0.13153941848143152 36-37 0.09077353298860648 38-39 0.08410377078835966 40-41 0.08374879744997117 42-43 0.07451949065187055 44-45 0.09716928068202708 46-47 0.14356491876964497 48-49 0.22318481580993954 50-51 0.17850799599241327 52-53 0.11135575901271078 54-55 0.05995312857308689 56-57 0.07409601368677551 58-59 0.11133084860299931 60-61 0.08652008053037252 62-63 0.09566842849691086 64-65 0.10562636477907206 66-67 0.1014538711524004 68-69 0.09093545065173106 70-71 0.12626463922502718 72-73 0.149798748799941 74-75 0.1941205952790788 76-77 0.1942389197252083 78-79 0.15511712127334043 80-81 0.09246744084898662 82-83 0.1319193022295315 84-85 0.161326040893925 86-87 0.17264159450536146 88-89 0.1598563267209481 90-91 0.1820888673884375 92-93 0.10131063629655941 94-95 0.3400955961883087 96-97 0.34129129585445944 98-99 0.3818329876598812 100 0.3842679802091777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8028772.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.35410930788938 #Duplication Level Percentage of deduplicated Percentage of total 1 75.39564733887384 27.409416046964985 2 13.368671608646176 9.720122979240008 3 4.314392415629493 4.705376804247706 4 1.9546728914154814 2.842415678227464 5 1.086174406730251 1.9743451554851723 6 0.7016546014535388 1.5304816846575302 7 0.4636308828826411 1.1798421456380155 8 0.3622085207043458 1.053421452314776 9 0.2889006554259014 0.9452453405826691 >10 1.7019459514813156 11.885635517710531 >50 0.17831749757431134 4.5352993045616765 >100 0.14755319078240703 11.151268362272804 >500 0.022123491180340748 5.525011801238079 >1k 0.012867923562042902 8.406099943094665 >5k 6.193118289255338E-4 1.5556414451283351 >10k+ 6.193118289255338E-4 5.580376338635579 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 57487 0.7160123615417152 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 54448 0.6781609939851324 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32810 0.4086552713167094 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 31835 0.3965114465823665 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 29678 0.36964556970854323 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 29550 0.368051303487009 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 24156 0.30086792849516714 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 22829 0.284339871651605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21991 0.27390240998249793 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 20121 0.25061117690227097 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 19579 0.24386045587046187 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 17496 0.21791626415596307 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 17211 0.21436653077207823 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 15655 0.19498623201655246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15595 0.1942389197252083 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 13558 0.16886766743407336 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 11421 0.1422508946573648 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 10722 0.13354470646320507 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 9741 0.12132615049972773 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 8960 0.11159863550739765 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 8878 0.11057730870922726 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8394 0.10454898955905088 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 8047 0.10022703347411036 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2455204855736345E-4 0.0 0.0 0.0 0.0 2 2.864697116819359E-4 0.0 0.0 1.2455204855736345E-5 0.0 3 2.864697116819359E-4 0.0 0.0 2.491040971147269E-5 0.0 4 3.487457359606176E-4 0.0 0.0 2.491040971147269E-5 0.0 5 0.0016440870409571975 0.0 0.0 3.736561456720903E-5 0.0 6 0.0028771523216750954 0.0 0.0 3.736561456720903E-5 1.2455204855736345E-5 7 0.0032757188770586587 0.0 0.0 3.736561456720903E-5 1.2455204855736345E-5 8 0.003699195842153694 0.0 0.0 7.473122913441806E-5 1.2455204855736345E-5 9 0.00398566555383563 0.0 0.0 8.71864339901544E-5 1.2455204855736345E-5 10-11 0.007037190743491035 0.0 0.0 1.4323485584096795E-4 1.2455204855736345E-5 12-13 0.01529499156284423 0.0 0.0 1.9305567526391334E-4 2.491040971147269E-5 14-15 0.024960230530895633 0.0 0.0 2.6155930197046325E-4 3.736561456720903E-5 16-17 0.028416549878362472 0.0 0.0 4.4215977237864023E-4 3.736561456720903E-5 18-19 0.030160278558165558 0.0 0.0 6.352154476425536E-4 3.736561456720903E-5 20-21 0.03822502370225484 0.0 0.0 8.967747496130168E-4 3.736561456720903E-5 22-23 0.04092780315594963 0.0 0.0 0.0023166681031669602 4.359321699507721E-5 24-25 0.05975384529539511 0.0 0.0 0.005972270728325577 4.982081942294538E-5 26-27 0.11717856728276752 0.0 0.0 0.011085132321605348 5.604842185081355E-5 28-29 0.12879304581074166 0.0 0.0 0.03209083531080469 7.473122913441806E-5 30-31 0.13090420303378897 0.0 0.0 0.09719419109173856 7.473122913441806E-5 32-33 0.1322057719412134 0.0 0.0 0.1938216703625411 7.473122913441806E-5 34-35 0.13315859511267725 0.0 0.0 0.31649921058911623 7.473122913441806E-5 36-37 0.13493968940704754 0.0 0.0 0.5128243771276604 7.473122913441806E-5 38-39 0.13696366019610473 0.0 0.0 0.7921198908126922 8.095883156228624E-5 40-41 0.13868247846619633 0.0 0.0 1.1624069035713058 8.71864339901544E-5 42-43 0.141958197343255 0.0 0.0 1.4502466877873728 8.71864339901544E-5 44-45 0.14662267156172826 0.0 0.0 1.7395686413812723 9.341403641802258E-5 46-47 0.14916353335229846 0.0 0.0 2.0765815743677862 9.964163884589076E-5 48-49 0.15089480682724582 0.0 0.0 2.397122498932589 1.120968437016271E-4 50-51 0.1532612957498357 0.0 0.0 2.7565485730570005 1.120968437016271E-4 52-53 0.15436980898199626 0.0 0.0 3.155750343888206 1.120968437016271E-4 54-55 0.1571161816526861 0.0 0.0 3.509640079454243 1.120968437016271E-4 56-57 0.16219790523382654 0.0 0.0 3.887119973017044 1.120968437016271E-4 58-59 0.16415337239617717 0.0 0.0 4.275143197490227 1.120968437016271E-4 60-61 0.16536775486961144 0.0 0.0 4.637184864634342 1.120968437016271E-4 62-63 0.16609638435367202 0.0 0.0 5.01873761018497 1.120968437016271E-4 64-65 0.16678764822316539 0.0 0.0 5.423438353959983 1.120968437016271E-4 66-67 0.1676283745509276 0.0 0.0 5.8307546907547 1.1832444612949527E-4 68-69 0.1684877836859734 0.0 0.0 6.298372901858466 1.3077965098523162E-4 70-71 0.16959006931570605 0.0 0.0 6.830316018439682 1.3700725341309977E-4 72-73 0.17250458725194837 0.0 0.0 7.299640592608682 1.6191766312457248E-4 74-75 0.17497694541581205 0.0 0.0 7.755090068568395 1.6191766312457248E-4 76-77 0.17576162332172343 0.0 0.0 8.255577565286448 1.6191766312457248E-4 78-79 0.17639683876936596 0.0 0.0 8.770232857527901 1.6191766312457248E-4 80-81 0.17741816556753637 0.0 0.0 9.329589879996593 1.6191766312457248E-4 82-83 0.17800356019575597 0.0 0.0 9.94974698496856 1.6191766312457248E-4 84-85 0.1803202282989229 1.2455204855736345E-5 0.0 10.540354116420295 1.6191766312457248E-4 86-87 0.18446781151588312 1.8682807283604516E-5 0.0 11.133900676217982 1.6814526555244063E-4 88 0.1854704555067699 2.491040971147269E-5 0.0 11.603729686183641 1.743728679803088E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 8025 0.0 27.820454 1 ATCTACA 9395 0.0 24.459064 2 TCTACAC 10890 0.0 23.871197 3 TACACTC 10605 0.0 22.848642 5 CTACACT 13145 0.0 21.059036 4 AGTACTT 37870 0.0 20.548174 12-13 GAGTACT 36440 0.0 20.283907 12-13 CACTCTT 12740 0.0 18.604649 7 TACTTTT 43760 0.0 17.89171 14-15 CGTTAAC 820 0.0 17.806507 1 GTACTTT 41795 0.0 17.743698 14-15 ACTCTTT 15625 0.0 17.48377 8 CATGGGG 18050 0.0 17.240347 4 CATGGGT 8240 0.0 17.083063 4 ACACTCT 14205 0.0 17.081158 6 GTATAAG 2555 0.0 16.775728 1 GTACTAG 2315 0.0 16.683752 1 GTATATA 1570 0.0 16.50038 1 ACTTTTT 45710 0.0 16.491512 16-17 GTATTAG 2975 0.0 16.307266 1 >>END_MODULE